• Journal of Ginseng Research
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• v.44 no.3
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• pp.519-526
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• 2020

Background: Bisphenol A (BPA), known as an endocrine disruptor, is widely used in the world. BPA is reported to cause inflammation-related diseases. Korean Red Ginseng (KRG) has been used safely in human for a long time for the treatment of diverse diseases. KRG has been reported of its mitigating effect on menopausal symptoms and suppress adipose inflammation. Here, we investigate the protective effect of orally administered KRG on the impacts of BPA in the liver and uterus of menopausal mice model. Methods: The transcriptome analysis for the effects of BPA on mice liver was evaluated by Gene Expression Omnibus (GEO) database-based data (GSE26728). In vivo assay to evaluate the protective effect of KRG on BPA impact in ovariectomized (OVX) mice were designed and analyzed by RNA sequencing. Results: We first demonstrated that BPA induced 12 kinds of gene set in the liver of normal mice. The administration of BPA and KRG did not change body, liver, and uterine weight in OVX mice. KRG downregulated BPA-induced inflammatory response and chemotaxis-related gene expression. Several gene set enrichment analysis (GSEA)-derived inflammatory response genes increased by BPA were inhibited by KRG in OVX mice. Conclusion: Our data suggest that BPA has commonly influenced inflammatory response effects on both normal and OVX mice. KRG protects against BPA impact of inflammatory response and chemotaxis in OVX mouse models. Our comparative analysis will provide new insight into the efficacy of KRG on endocrine disrupting chemicals and OVX mouse.

• Korean Journal of Acupuncture
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• v.24 no.2
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• pp.141-161
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• 2007

Objectives & Methods : The purpose of this study is to investigate the anti-oxidative effects of electroacupuncture at Hyunjong (GB39) in rats. The author performed several experimental items including change of body weight, relative liver weight, level of albumin, total bilirubin, LDL-Cholesterol, LDH, GOT and GPT in blood serum, level of SOD, glutathione, catalase, NO and MDA in liver, histological change of liver. Results : 1. In the GB39-EA group, body weights were decreased in the early stages, but increased later on gradually. 2. In the GB39-EA group, the level of LDL-cholesterol was significantly decreased in comparison with that of the holder group, sham-EA group and GB39-NR group. 3. In the GB39-EAgroup, liver SOD, glutathione, catalase activity were increased, and NO density was decreased in comparison with those of the holder group, sham-EA group and GB39-NR group. MDA activity was significantly decreased in comparison with that of the holder group. 4. In the GB39-EA group, the density of liver tissue was more similarly maintained to the normal group in comparison with that of the holder group and sham-EA group. 5. The results of the GB39-EA group showed similar tendency with those of the GB39-EA group, but the effects were lower than those of the GB39-EA group. Conclusions : These results suppose that electroacupuncture at GB39 has an anti oxidative effect in human.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.8
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• pp.3721-3725
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• 2016

The liver fluke Opisthorchis viverrini is a serious health problem in Thailand. Infection is associated with cholangiocarcinoma (CCA), endemic among human populations in northeast and north Thailand where raw fish containing fluke metacercariae are frequently consumed. Recently, Thailand public health authorities have been organized to reduce morbidity and mortality particularly in the northeast through O. viverrini and CCA screening projects. Health modification is one of activities included in this campaign, but systemic guidelines of modifying and developing health behavior among liver fluke and CCA prevention in communities towards health belief and social support theory are still various and unclear. Here we review the guidelines for modifying and developing health behavior among populations in rural communities to strengthen understanding regarding perceived susceptibility, severity, benefits, and barriers to liver fluke and CCA prevention. This model may be useful for public health officers and related organizations to further health behavior change in endemic areas.

• Journal of Physiology & Pathology in Korean Medicine
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• v.30 no.6
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• pp.452-457
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• 2016

If human body is exposed to the continuous stress, it becomes allostasis load which is the condition of homeostasis broken. Its evolutional ecologic point of view and the relation with chiljeongsnag which is a theory in Oriental Medicine were investigated. Upon evolutional ecologic point of view by Maynard Smith, people can be divided by Hawks and Doves resulting in different types of allostasis in response of the stress. Hawks people who are active and aggressive get easily anger in the stressful situation to be vulnerable to the inflammatory hepatic diseases by enhancing Th1 immune system. On the other hand, Doves people who are passive and calm get easily depressed with sadness in the stressful situation to be vulnerable to the allergic pulmonary diseases by enhancing Th2 immune system. According to constitution theory of Oriental Medicine, Yangin and Eumin show the different features of responses to the stress generating Chiljeongsang. With excessive stress continuously, Yangin consider the feeling of anger mainly resulting in Qi reversal and liver damage, while Eumin consider the feeling of sadness mainly in consumption of Qi and lung damage. Hawks and Yangin, and Doves and Eumin show the common behaviors in response to the stress demonstrating the similar features including allostasis load and Chiljeongsang. In the clinical practices with the stressful patients, the viewpoint to consider the behaviors and feelings of the subjects to receive the stress simultaneously can be the new approaching method in Psychosomatic Medicine.

• Korean Journal of Veterinary Research
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• v.38 no.3
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• pp.606-613
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• 1998

Cadmium is one of the well-known environmental toxicants and induces cancer in rodents and human, but its carcinogenic mechanism has not been well demonstrated until now. Genotoxic effects of cadmium in Salmonella typhimurium TA98, TA100 and TA1535/pSK1002 or in WB-F344 rat liver epithelial cells were investigated to elucidate the tumor initiating effects of cadmium. TA98, TA100 and TA1535/pSK1002 tester strains were used to detect frameshift mutation, base-pair mutation and SOS repair response, respectively, in Salmonella mutation test. Reverse mutations from histidine to $histidin^+$ of Salmonella typhimurium TA98 and TA100 by $CdCl_2$ were not significantly different from control up to the maximum doses ($100{\mu}M$ and $200{\mu}M$ in TA98 and TA100, respectively) at which non-cytotoxicity was observed. DNA SOS repair responses(${\beta}$-galactosidase activity) generally did not show significant increases compared to control in both of the conditions with or without metabolic activation in Salmonella typhimurium TA1535/pSK1002 by $CdCl_2$. But the activities of ${\beta}$-galactosidase by $400{\mu}M$ of $CdCl_2$ in metabolic activation condition and by 130 and $400{\mu}M$ of $CdCl_2$ in non-metabolic activation condition were more decreased than those of control. DNA single strand breaks for 4hrs were observed only in WB-F344 rat liver epithelial cells treated with $200{\mu}M$ of $CdCl_2$. As a conclusion, $CdCl_2$ did not induce gene mutation in microbials but induce DNA single strand breaks in rat liver epithelial cells.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3131-3135
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• 2015

Objective: To investigate whether the expression of serum soluble neural cell adhesion molecule (sNCAM) is associated with hepatic encephalopathy (HE) in hepatocelular carcinoma (HCC) patients. Materials and Methods: The Oncomine Cancer Microarray database was used to determine the clinical relevance of NCAM expression in different kinds of human cancers. Sera from 75 HCC cases enrolled in this study were assessed for expression of sNCAM by enzyme linked immunosorbent assay (ELISA). Results: Dependent on the Oncomine Cancer Microarray database analysis, NCAM was down regulated in 10 different kinds of cancer, like bladder cancer, brain and central nervous system cancer, while up-regulated in lung cancer, uterine corpus leiomyoma and sarcoma, compared to normal groups. Puzzlingly, NCAM expression demonstrated no significant difference between normal and HCC groups. However, we found by quantitative ELISA that the level of sNCAM in sera from HCC patients with HE ($347.4{\pm}151.9ng/ml$) was significantly more up-regulated than that in HCC patients without HE ($260.3{\pm}104.2ng/ml$), the p-value being 0.008. sNCAM may be an important risk factor of HE in HCC patients, the correlation coefficients was 0.278 (P<0.05) on rank correlation analysis. Conclusions: This study highlights that up-regulated level of serum sNCAM is associated with HE in HCC patients and suggests that the high expression can be used as an indicator.

• The Journal of Internal Korean Medicine
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• v.24 no.1
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• pp.21-32
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• 2003

Objective : The aim of this study is to investigate the inhibitory effect of Injinchunggantang on hepatic sclerosis induced by $CCl_4$. Methods : Weight, liver function test and complete blood cell count, gross findings, and findings on liver tissue of the past(Hematoxylin & Eosin stain, Masson-Trichrome stain) were studied. Results : When it comes to the change of rats' body weight, The $CCl_{4^+}$Injinchunggantang group lost far less weight than The $CCl_{4^-}$only group. In the liver function test, which is focused on various areas such as total cholesterol, alkaline phosphotase, albumin, aspartate transaminase, alanine transaminase, The $CCl_{4^+}$Injinchunggantang group was much more closer to normal limit than the $CCl_4$ only group. In the complete blood cell count, including white blood cell, red blood cell, hemoglobin, hematocrite, platelet, The $CCl_{4^+}$Injinchunggantang group significantly closer to normal limit than $CCl_{4^-}$only group. In the gross findings of hepatic fibrosis models, Injinchunggantang showed inhibitory effect on hepatic fibrosis in the order. In the past findings of hepatic fibrosis models in Hematoxylin & Eosin, Masson-Trichrome staining, the liver in $CCl_{4^-}$only group showed atrophy and necrotic change with white nodules, whereas that of $CCl_{4^+}$Injinchunggantang group showed lesser significant change with the well_preserved tone of the tissue. In the extent of the inhibition of the hepatic fibrosis, the Injinchunggantang group showed statistically significant inhibitory effect(p<0.05) in the sclerosis model. Conclusions : These results show that Injinchunggantang have inhibitory effect on hepatic sclerosis induced by $CCl_4$ and further ultimately prevent liver cirrhosis. To obtain more credible results in this experiment, the invention of a new experimental model more similar to human hepatic sclerosis is still needed.

• Journal of Physiology & Pathology in Korean Medicine
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• v.38 no.1
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• pp.10-15
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• 2024

Previous studies have highlighted the pivotal role of the β-adrenergic receptor (β-AR) signaling pathway in stimulating cancer metastasis induced by chronic stress. According to the theory of traditional Korean medicine, chronic stress can induce Qi stagnation. Based on the traditional role of premature citrus unshiu peel in moving Qi, we hypothesized that an ethanol extract of premature citrus unshiu peel (EPCU) can attenuate chronic stress-induced cancer progression. In this study, we investigated the potential role of EPCU on modulating the adrenergic agonists-induced metastatic properties of liver cancer cells. Our findings revealed that adrenergic agonists, including norepinephrine (NE), epinephrine (E), and isoproterenol (ISO), augmented the migratory capacity of Hep3B human hepatocellular carcinoma cells, which was completely abrogated by EPCU treatment in a concentration-dependent manner. Consistently, EPCU inhibited the E-induced invasive property of Hep3B cells in a dose-dependent manner. These results suggest that EPCU efficiently attenuates adrenergic agonists-induced metastatic abilities of liver cancer cells. As a molecular mechanism, EPF suppressed the phosphorylation of major components of β-AR signaling pathway, including Src, signal transducer and activator of transcription 3 (STAT3) and ERK, induced by E treatment. Taken together, our results demonstrate that EPCU impedes the adrenergic agonists-driven metastatic potential of cancer cells by inhibiting β-AR signaling pathway. This study provides basic evidence supporting the probable use of premature citrus unshiu peel to prevent metastasis in liver cancer patients under chronic stress.

• Animal Bioscience
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• v.37 no.8
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• pp.1317-1332
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• 2024

Objective: Rare study of the non-coding and regulatory regions of the genome limits our ability to decode the mechanisms of fatty liver hemorrhage syndrome (FLHS) in chickens. Methods: Herein, we constructed the high-fat diet-induced FLHS chicken model to investigate the genome-wide active enhancers and transcriptome by H3K27ac target chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-Seq) profiles of normal and FLHS liver tissues. Concurrently, an integrative analysis combining ChIP-seq with RNA-Seq and a comparative analysis with chicken FLHS, rat non-alcoholic fatty liver disease (NAFLD) and human NAFLD at the transcriptome level revealed the enhancer and super enhancer target genes and conservative genes involved in metabolic processes. Results: In total, 56 and 199 peak-genes were identified in upregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥0.5 and log2(FoldChange) ≥1) (PP) and downregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥0.5 and log2(FoldChange)≤-1) (PN), respectively; then we screened key regulatory targets mainly distributing in lipid metabolism (PCK1, APOA4, APOA1, INHBE) and apoptosis (KIT, NTRK2) together with MAPK and PPAR signaling pathway in FLHS. Intriguingly, PCK1 was also significantly covered in up-regulated super-enhancers (SEs), which further implied the vital role of PCK1 during the development of FLHS. Conclusion: Together, our studies have identified potential therapeutic biomarkers of PCK1 and elucidated novel insights into the pathogenesis of FLHS, especially for the epigenetic perspective.

• The Journal of Dong Guk Oriental Medicine
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• v.7 no.2
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• pp.81-95
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• 1999

Among oriental medicine,s literatures, through Hwang-Je-Nae-Kyung(黃帝內徑) to Chung(淸), I extracted contents related to atrophy syndrome(?證). And studied it,s pathophysiology, therapy and treatment. Then, I concluded that result same below 1. The pathophysiologies of atrophy syndrome are lung heat(肺熱) & decreasing of it,s circulation, making liver and stomach weaken & difficulty it's circulation, injurious to liver and kidney & atrophy of bonemarrow and muscle. 2. The most important point of atrophy syndrome therapy is Yang-Myung(陽明). The Priority of therapy is stomach & liver,s balance. And then we must protect acquired human function & clean humidity & temperature of Yang-Myung(陽明). under the principle of decreasing south organ,s function & protecting north organ,s function, we should Ja-Yeum-Chung-Yeul(滋陰淸熱). so remove temperature of lung & protect liver and kidney & make strong stomach. 3. Among the therapy of atrophy syndrome in literatures Yi-Jin-Tang(二陳湯), Sa-Gun-Ja-Tang(四君子湯), Sa-Mul-Tang-Je(四物湯劑), Ho-Jam-Hwan(虎蠶丸), Dong-Won-Geun-Bo-Hwan(東垣健步丸) and Chung-Jo-Tang(淸燥湯) were many. These make strong spleen & dry humidity organ using Sa-Gun-Ja-Tang(四君子湯)and Yi-Jin-Tang(二陳湯) by Dog-Cheu-Yang-Myung(獨取陽明) method. Sa-Mul-Tang(四物湯), Phellodendri cortex(黃柏), Radix sophorae flavescentis(苦蔘), Carapax Testudinis(龜板) bitter taste make strong Yeum(陰) & decrease Yang(陽) so important human muscle powerful. Ho-Jam-Hwan(虎蠶丸), Dong-Won-Geun-Bo-Hwan(東垣健步丸), Chung-Jo-Tang(淸燥湯) make Chung-Yeul-Jo-Seup(淸熱燥濕), protect liver and kidney & strong muscle and bone. Besides Gum-Gang-Hwan(金剛丸), Yi-Myo-Hwan(二妙丸), Nok-Gak-Geu-Hwan(鹿角膠丸)&Ga-Mi-Sa-Geun-Hwan(加味四斤丸) were used in treatment of atrophy syndrome.