• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2555-2559
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• 2015

Purpose: To investigate effects of the TESTIN (TES) gene on proliferation and migration of highly metastatic nasopharyngeal carcinoma cell line 5-8F and the related mechanisms. Materials and Methods: The target gene of human nasopharyngeal carcinoma cell line 5-8F was amplified by PCR and cloned into the empty plasmid pEGFP-N1 to construct a eukaryotic expression vector pEGFP-N1-TES. This was then transfected into 5-8F cells. MTT assays, flow cytometry and scratch wound tests were used to detect the proliferation and migration of transfected 5-8F cells. Results: A cell model with stable and high expression of TES gene was successfully established. MTT assays showed that the OD value of 5-8F/TES cells was markedly lower than that of 5-8F/GFP cells and 5-8F cells (p<0.05). Flow cytometry showed that the apoptosis rate of 5-8F/TES cells was prominently increased compared with 5-8F/GFP cells and 5-8F cells (p<0.05). In vitro scratch wound assays showed that, the width of the wound area of 5-8F/TES cells narrowed slightly, while the width of the wound area of 5-8F/ GFP cells and 5-8F cells narrowed sharply, suggesting that the TES overexpression could inhibit the migration ability. Conclusions: TES gene expression remarkably inhibits the proliferation of human nasopharyngeal carcinoma cell line 5-8F and reduces its migration in vitro. Thus, it may be a potential tumor suppressor gene for nasopharyngeal carcinoma.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.8
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• pp.884-890
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• 2003

In order to reduce the specific absorption rate(SAR) in a human head exposed to electromagnetic fields radiated by a cellular phone, we have analyzed an electromagnetic wave absorber attached to the handset. A manufactured electromagnetic wave absorber was composed of Mn - Zn, which had complex relative permittivity of 7.30-j0.05 and permeability of 2.20-i1.55. The SAR value from the electromagnetic wave absorber attachment was calculated by using the nonuniform finite difference time domain(FDTD) algorithm and measured by phantom model at 835 MHz. The SAR reduction due to the electromagnetic wave absorber are about 18 % at 835 MHz. The V.S.W.R and radiation pattern of antenna are good agreement with the normal antenna. The gain reduction due to the electromagnetic wave absorber are only 0.3 dB at 835 MHz. But the sensitivity of cellular phone generally improves about 1 dB.

• Journal of Astronomy and Space Sciences
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• v.32 no.1
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• pp.81-89
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• 2015

In this paper, we describe the development of a bioreactor for a cell-culture experiment on the International Space Station (ISS). The bioreactor is an experimental device for culturing mouse muscle cells in a microgravity environment. The purpose of the experiment was to assess the impact of microgravity on the muscles to address the possibility of long-term human residence in space. After investigation of previously developed bioreactors, and analysis of the requirements for microgravity cell culture experiments, a bioreactor design is herein proposed that is able to automatically culture 32 samples simultaneously. This reactor design is capable of automatic control of temperature, humidity, and culture-medium injection rate; and satisfies the interface requirements of the ISS. Since bioreactors are vulnerable to cell contamination, the medium-circulation modules were designed to be a completely replaceable, in order to reuse the bioreactor after each experiment. The bioreactor control system is designed to circulate culture media to 32 culture chambers at a maximum speed of 1 ml/min, to maintain the temperature of the reactor at $36{\pm}1^{\circ}C$, and to keep the relative humidity of the reactor above 70%. Because bubbles in the culture media negatively affect cell culture, a de-bubbler unit was provided to eliminate such bubbles. A working model of the reactor was built according to the new design, to verify its performance, and was used to perform a cell culture experiment that confirmed the feasibility of this device.

• Fire Science and Engineering
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• v.5 no.3
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• pp.5-14
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• 1991

Now a days, according that the occurrence of industrial disaster is on the increase, the necessity of protective goods is increasing. Specially estimate of safety helmet for protect of head is very important. On this, in this paper, amplification ratio and natural frequencies of fire safety helmet and general safety helmet are estimated by falling impect test and frequency analysis. Also. trend of damping is estimated by using these test results. And we know that the fire safety helmet is more safe than general safety helmet for protect of head.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.15 no.1
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• pp.31-36
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• 2004

Background and Objectives : It has been shown that the epilaryngeal tube in the human airway is responsible for vocal ring, or the singer's formant. In previous study, authors showed that in trained tenors, besides the conventional singer's formant in the region of ,5500Hz, another energy peak was observed in the region of 8,000Hz. This peak was interpreted as the second resonance of the epilarynx tube. Singers in other voice categories who produce vocal ring are assumed to have the same peak, but no measurements have as yet been made. Materials and Methods : Fifteen tenors, fourteen baritones, seven sopranos and five mezzo sopranos attending the music college, department of vocal music who could reliably produce the head and chest registers were chosen for this study. Each subject was asked to produce an/ah/sound for at least three seconds for the head register sound(tenors ; G4, barions ; E4 sopranos ; F5 and mezzosopranos ; C5) and for the chest register sound (tenors ; C3, baritones ; D3, sopranos ; D4 and Mezzosoprano ; A3). The sound data was analyzed using the Fast Fourier Transform (FFT)-based power spectrum, Long term average(LTA) power spectrum using the FFT algorithm of the Computerized Speech Lab (CSL, Kay elemetrics, Model 4300B, USA). Statistical analysis was performed using the Mann-Whitney test of the Statistical Package for Social sciences(SPSS). Results : For head register sounds, a significant increase was seen in the 2,200-3,400Hz region(p<0.05) and the Similar to the head register sounds, there was a significant increase in energy in the four trained singer group compared with the untrained group in the 2,200-3,100Hz region(p<0.05), the 7,800-8,400Hz region(p<0.05) for the chest register sounds. Conclusions : When good vocal production was made for the head and chest registers, an energy peak was observed near 2,500Hz, a frequency already known as the "singer's formant', in all subjects in the study group. Another region of increased energy was observed around 8,000Hz that had not been noticed previously. The authors believe this region to be the second singer's formant.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• v.9 no.2
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• pp.705-709
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• 2005

In this paper, we introduce fuzzy algorithm similar to human's way of thinking and designed collision detection system of vehicles. First, before the model vehicles design, we did simulation collision detection using PID and Fuzzy Controller. As a result, P.O that is Percent Overshoot when make use of PID controller happened from smallest 32% to 45%. But, In case of using fuzzy controller they produced about 10% in 7% in case use 25 rule. We designed model vehicles that introduce Auto Guided Vehicle(AGV) with confirmed result in simulation. We set Polaroid 6500 sensor on the front of model automobile because distinguish existence automobile to the head. And we composed motor drive part to run vehicles and 80C196KC processor for control movement of vehicles influenced on distance data of the front vehicles that receive from supersonic waves sensor. In case of using Fuzzy controller, last value percent error happened about maximum 15% in smallest 5%, and we confirmed that distance with front vehicles kept when state hold time is about maximum 16 seconds in smallest 10 seconds.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.5
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• pp.115-123
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• 2010

Cool-down performance after soaking is important because it affects passenger's thermal comfort. The cooling capacity of HVAC system determines initial cool down performance in most cases, the performance is also affected by location, and shape of panel vent, indoor seat arrangement. Therefore, optimal indoor designs are required in developing a new car. In this paper, initial cool down performance is predicted by CFD(computational fluid dynamics) analysis. Experimental time-averaging temperature data are used as inlet boundary condition. For more reliable analysis, real vehicle model and human FE model are used in grid generation procedure. Thermal and aerodynamic characteristics on re-circulation cool vent mode are investigated using CFX 12.0. Thermal comfort represented by PMV(predicted mean vote) is evaluated using acquired numerical data. Temperature and velocity fields show that flow in passenger's compartment after soaking is considerably unstable at the view point of thermodynamics. Volume-averaged temperature is decreased exponentially during overall cool down process. However, temperature monitored at different 16 spots in CFX-Solver shows local variation in head, chest, knee, foot. The cooling speed at the head and chest nearby panel vent are relatively faster than at the knee and foot. Horizontal temperature contour shows asymmetric distribution because of the location of exhaust vent. By evaluating the passenger's thermal comfort, slowest cooling region is found at the driver's seat.

• Korean Journal of Computational Design and Engineering
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• v.12 no.2
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• pp.87-94
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• 2007

The functional behavior of a consumer electronic product is nearly all expressed with human-machine interaction (HMI) tasks. Although physical prototyping and computer aided design (CAD) software can show the appearance of the product, they cannot properly reflect its functional behavior. In this paper, we propose an approach to virtual prototyping (VP) that incorporates HMI functional simulation into virtual reality techniques in order to enables users to capture not only the realistic look of a consumer electronic product but also its functional behavior. We adopt state transition methodology to capture the HMI functional behavior of the product into a state transition chart, which is later used to construct a finite state machine (FSM) for the functional simulation of the product. The FSM plays an important role to control the transition between states of the product. We have developed a VP system based on the proposed approach. The system receives input events such as mouse clicks on buttons and switches of the virtual prototype model, and it reacts to the events based on the FSM by activating associated activities. The system provides the realistic visualization of the product and the vivid simulation of its functional behavior using head-mounted displays (HMD) and stereo speakers. It can easily allow users to perform functional evaluation and usability testing. A case study about the virtual prototyping of an MP3 player is given to show the usefulness of the proposed approach.

• Annual Conference on Human and Language Technology
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• 2014.10a
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• pp.81-86
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• 2014

한국어 의존 파싱은 문장 내 단어의 지배소를 찾음으로써 문장의 구조적 중의성을 해소하는 작업이다. 지배소 후위 원칙은 단어의 지배소는 자기 자신보다 뒤에 위치한다는 원리로, 한국어 구문분석을 위하여 널리 사용되는 원리이다. 본 연구에서는 한국어 지배소 후위 원리를 의존 파싱을 위한 트랜지션 시스템의 제약 조건으로 적용하여 2가지 트랜지션 시스템을 제안한다. 제안 모델은 기존 트랜지션 시스템 중 널리 사용되는 arc-standard와 arc-eager 알고리즘에 지배소 후위 제약을 적용한 포워드(forward) 기반 트랜지션 시스템과, 트랜지션 시스템의 단점인 에러 전파(error propagation)를 완화시키기 위하여 arc-eager 알고리즘의 lazy-reduce 방식을 적용한 백워드(backward) 기반 트랜지션 시스템이다. 실험은 세종 구구조 말뭉치를 의존구조로 변환하여 실험하였고, 실험 결과 백워드 기반 트랜지션 시스템이 포워드 방식보다 우수한 성능을 보였다. 기존 연구와의 비교를 위하여 기존 연구를 조사하였지만 세부 실험 환경이 서로 달라서 직접적인 비교는 어려웠다. 제안하는 시스템의 최고 성능은 UAS 92.85%, LAS 90.82% 이다.

• Annual Conference on Human and Language Technology
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• 1999.10e
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• pp.203-208
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• 1999

한국어는 잉여성과 중의성의 범 언어적인 특징과 함께 다른 언어에 비해 주어의 생략이 두드러지며 어순이 자유롭기 때문에 구문 형식의 지배를 덜 받는다는 개별적인 특성을 지닌다. 이러한 특성으로 인해 기계번역의 패턴을 추출할 때 서로 유사 가능성이 있는 패턴에 대한 고려가 없이는 같은 의미의 서로 다른 여러 개의 패턴을 모두 하나의 패턴으로 처리하는 오류를 범할 위험이 있다. 본 연구에서 사용되는 구 단위 패턴은 동사구, 명사구, 형용사구 그리고 부사구를 중심으로 한국어 패턴, 패턴 대표 카테고리, 한국어 패턴의 중심어 및 제약조건 대역영어패턴 의미코드로 나뉜다. 범 언어적인 특성의 한국어와 영어간 격차를 해소하기 위해 각각의 명사에 의미코드를 사용하여 다중 언어기반 체계를 구축하였으며. 한국어의 개별적인 특성으로 인해 발생하는 문제를 해소하기 위해 중심어 부과 자질을 사용하였다. 중심어 부과 자질에 있어서, 특히 술어기능명사를 중심어로 하는 기능동사 '하-' 구문은 다른 동사 구문의 형식과는 달리 논항의 수와 형태를 동사가 아닌 명사가 수행하게 된다. 이러한 특징에 대한 변별적인 자질 부여는 구문의 형태-통사적 특징 뿐만이 아니라 의미적인 고유의 특성까지도 잘 뒷받침하면서 패턴 추출에 월등한 효율성을 제시할 수 있다. 향후 이에 대한 연구는 전반적인 기능동사 구문뿐만이 아니라 개별적인 특징을 보이는 모든 구문에 대한 연구로 확대되어 패턴 기반 기계번역의 패턴 추출에 기본적인 정보의 역할을 담당해야 할 것이다.