• 한국작물학회지
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• 제38권1호
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• pp.46-54
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• 1993

최근 육성된 내한성이며 찰쌀보리인 품종들이 확대 보급을 통하여 재배지대의 북상과 수량에 대한 안정성을 제고시키기 위하여 찰과, 찰피, 메과 및 메피성의 isogenic line을 이용한 찰ㆍ메 및 피ㆍ과성인자가 발아 및 출현률에 미치는 영향을 검사한 결과는 다음과 같다. 1.수확 전일 강우시는 피성에 비하여 과성의 발아율이 4-6% 낮았으며, 5일 인공강수처리에서는 과성의 발아율로 현저히 저하되었다. 2. 탈곡기회전속도가 600rpm에서 1000rpm으로 상승할 때 피성의 발아율이 100%에서 90%로 저하되었으나, 과성은 98%에서 76%로 크게 저하되었다. 3. 과성에서 발아율보다 출현률이 피성에 비하여 큰 차이가 있었으며 파종심도가 깊을수록 그 차이가 컸다. 4. 파종심도, 고탈곡회전수가 수확시의 강우순으로 피성보다 과성의 출현율은 크게 저하되었다. 5. 탈곡시 찰성배유유부자에 따른 쌀보리의 배손상정도와는 관련이 없었으며, 6. 탈곡시 배의 손상은 겉보리는 영에 의한 보호로 적었으나 영의 보호가 없는 쌀보리는 컸다. 7. 탈곡시 탈곡시의 회전수를 600, 900rpm으로 탈곡만 하거나 또는 탈곡후 탈망회전속도를 750rpm으로 탈망한 종자의 손상입률 및 발아율은 겉보리는 큰 영향이 없었으며, 쌀보리는 매우 높아 채종시 주의가 필요하다. 8. 수발아율은 찰성인자 도입시에는 메성에 비해 높았으나 과성인자 도입시에는 차이가 없었다. 9. 과맥의 출현률 향상을 위하여는 수확후 강우피해를 방지하고 탈곡시 회전속도를 낮추며 파종심도를 적절히 조절하여 찰성인자 도입시에는 등숙과 정중 휴안성이 낮아 수발아률이 높은 점을 고려한 육종적 조치를 하여야 찰과성 품종의 육성보급시 재배면적의 북상 및 확대를 기하고 수량의 안정성을 제고할 수 있다.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.12-17
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• 2021

Saponarin found in young barley sprouts has a variety of beneficial biological and pharmacological properties, including antioxidant, hypoglycemic, antimicrobial, and hepatoprotective activities. Our previous work demonstrated that saponarin content was correlated with the expression levels of three biosynthetic pathway genes [chalcone synthase (HvCHS1), chalcone isomerase (HvCHI), and UDP-Glc:isovitexin 7-O-glucosyltransferase (HvOGT1)] in young barley seedlings under various abiotic stress conditions. In this study, we investigated the saponarin content and expression levels of three saponarin biosynthetic pathway genes in hulled and hulless domestic barley cultivars. In the early developmental stages, some hulled barley cultivars (Kunalbori1 and Heukdahyang) had much higher saponarin contents than did the hulless barley cultivars. An RNA expression analysis showed that in most barley cultivars, decreased saponarin content correlated with reduced expression of HvCHS1 and HvCHI, but not HvOGT1. Heat map analysis revealed both specific increases in HvCHS1 expression in certain hulled and hulless barley cultivars, as well as general changes that occurred during the different developmental stages of each barley cultivar. In summary, our results provide a molecular genetic basis for the metabolic engineering of barley plants to enhance their saponarin content.

• 한국육종학회지
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• 제43권5호
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• pp.375-382
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• 2011

A chemical, MNU-induced hulless barley mutant line designated as 'Mutant 98 (M98)' was developed from a Korean hulless waxy barley cultivar, 'Chalssalbori'. The objective of the study was to determine the genetic basis of 'M98' and the possibility of using 'M98' as breeding parent to improve lysine level. Compared to 'Chalssalbori', 'M98' had large embryo and higher lysine content in both the embryo and endosperm. Significantly different lysine content in 'M98' and the other high-lysine barley mutant stocks was observed for two years. However, the genotype by year interaction was not significant. 'M98' was higher than the other high-lysine barley mutant stocks in the percentage of lysine of total amino acid composition (0.75%). The trait of shrunken endosperm of 'M98', which was typical in the high-lysine mutants, was inherited by a single recessive gene. Based on seed morphology and lysine content of $F_1$ seeds, 'M98' had a genetically different gene from the other high-lysine mutants for shrunken endosperm. Segregation of $F_2$ for plump/shrunken endosperm did not fit the expected ratio of Mendelian inheritance except for only one cross combination (GSHO1784 (lys1)/M98). The amino acid analysis of $F_5$ and $F_6$ progenies from the cross between 'M98' and 'Chalssalbori' revealed that the attempt to increase the range of lysine content of plump lines did not go beyond the limit of the average high-lysine barley germplasm.

• 한국작물학회지
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• 제51권4호
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• pp.335-339
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• 2006

The level of ${\beta}-glucan$ which is a major soluble dietary fiber found in the grain endosperm cell wall was highly variable among 25 barley genotypes grown at four locations including Suwon, Naju, Jinju, and Jeju. Statistically significant genotypic effects were observed for ${\beta}-glucan$ content at each or across growing sites (P<0.001). On average, 'Chalssalbori' showed the lowest percentage ${\beta}-glucan$ (4.04%) among genotypes in the grain, whereas 'Yonezawa Mochi' was highest in percentage ${\beta}-glucan$ (6.46%) compared to other genotypes. The significant difference between genotypes was approximately 1-2% across environments. The effects of location or interaction between locations and genotypes were not significant on the variation of ${\beta}-glucan$ contents. High ${\beta}-glucan$ content seemed to be greatly associated with such grain traits as waxiness and presence of husk except for 'Chalssalbori'. The waxy genotypes had a mean of 5.37% and values ranging from 5.28 to 5.47%, but normal genotypes had a mean of 4.78% and values ranging from 4.69 to 4.88% over environments. Hulless barley genotypes were also higher than hulled barley genotypes for the average ${\beta}-glucan$ content in both individual and over all environments. The difference between the hulled and hulless gene pools was on average of 0.37% with ranges from 0.19% to 0.56% at four environments. ${\beta}-glucan$ content measured from a mapping population of $F_5$-derived 107 lines derived from the cross between 'Yonezawa Mochi' and 'Neulssalbori' was not significantly associated with other agronomic traits except for 1,000-kernel weight at the '01 Suwon environment. Not too much information on the relationship of ${\beta}-glucan$ content to agronomic traits was available.