• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.12
• /
• pp.1827-1834
• /
• 2014

Colon cancer is the third highest cause of death in Korea. Known dietary causes of colon cancer include a diet rich in fat and red meat as well as inadequate intake of dietary fiber, fruits, and vegetables. Therefore, recent research has focused on the anticancer effects of natural products. Opuntia humifusa is a type of prickly pear that is known to contain biologically active compounds that can be used in the treatment of diabetes mellitus, arteriosclerosis, and hyperglycemia. The aim of this study was to determine whether or not O. humifusa extract affects proliferation, cell death, and DNA fragmentation in human carcinoma HT-29 cells. O. humifusa is rich in carbohydrates, minerals (Mg, K, and Ca), and total phenolics. HT-29 cells were treated with extracts of O. humifusa at concentrations of 0, 0.25, 0.5, 1, and 2 mg/mL for 24 or 48 hours. O. humifusa extracts inhibited HT-29 cell growth in a dose-dependent manner. Hoechst 33342/PI double staining and Comet assay were performed to observe changes in nuclei of cancer cells undergoing cell death. The results of both tests showed that O. humifusa extract induced cell shrinkage, DNA fragmentation, and chromatin condensation dose-dependently in HT-29 cells. The results of this study suggest that O. humifusa extract inhibits the growth of HT-29 via induction of DNA fragmentation and chromatin condensation.

• Journal of Ginseng Research
• /
• v.29 no.1
• /
• pp.37-43
• /
• 2005

We performed in vitro and in vivo studies to know whether the inhibitory effects of ginsenosides on $5-HT_{3A}$ receptor channel acctivity are coupled to anti-nausea and anti-vomiting action. In vitro study, we investigated the effect of compound K (CK) and M4, which are ginsenoside metabolites, on human $5-HT_{3A}$ receptor channel activity expressed in Xenopus oocytes using two-electrode voltage clamp technique. Treatment of CK or M4 themselves had no effect in both oocytes injected with $H_2O\;and\;5-HT_{3A}$ receptor cRNA. In oocytes injected with $5- HT_{3A}$ receptor cRNA, M4 treatment inhibited more potently 5-HT-induced inward peak current $(I_{5-HT})$ than CK with dose-dependent and reversible manner. The half-inhibitory concentrations $(IC_{50})$ of CK and M4 were $36.9\;\pm\;10.1\;and\;7.3\;\pm\;2.2\;{\mu}M$, respectively. The inhibition of $I_{5-HT}$ by M4 was non-competitive and voltage-independent. These results indicate that M4 might regulate $5-HT_{3A}$ receptors. In vivo experiments, injection of cisplatin (7.5 mg/kg, i.v.) induced both nausea and vomiting with 1 h latency. These episodes reached to peak after 2 h and persisted for 4 h. Pre-treatment of GTS (500 mg/kg, p.o.) significantly reduced cisplatin-induced nausea and vomiting by $51\;\pm\;8.4\;and\;48.8\;\pm\;6.4\%$ during 4 h compared to GIS­untreated group, respectively. These results show the possibility that in vitro inhibition of $5-HT_{3A}$ receptor channel activity by ginsenosides might be coupled to in vivo anti-emetic activity.

• The Korean Journal of Physiology and Pharmacology
• /
• v.7 no.6
• /
• pp.295-301
• /
• 2003

Striatum plays a crucial role in the movement control and habitual learning. It receives an information from wide area of cerebral cortex as well as an extensive serotonergic (5-hydroxytryptamine, 5-HT) input from raphe nuclei. In the present study, the effects of 5-HT to modulate synaptic transmission were studied in the rat corticostriatal brain slice using in vitro extracellular recording technique. Synaptic responses were evoked by stimulation of cortical glutamatergic inputs on the corpus callosum and recorded in the dorsal striatum. 5-HT reversibly inhibited coticostriatal glutamatergic synaptic transmission in a dose-dependent fashion (5, 10, 50, and $10{\mu}M$), maximally reducing in the corticostriatal population spike (PS) amplitude to $40.1{\pm}5.0$% at a concentration of $50{\mu}M$ 5-HT. PSs mediated by non-NMDA glutamate receptors, which were isolated by bath application of the NMDA receptor antagonist, d,l-2-amino-5-phospohonovaleric acid (AP-V), were decreased by application of $50{\mu}M$ 5-HT. However, PSs mediated by NMDA receptors, that were activated by application of zero $Mg^{2+}$ aCSF, were not significantly affected by $50{\mu}M$ 5-HT. To test whether the corticostriatal synaptic inhibitions by 5-HT might involve a change in the probability of neurotransmitter release from presynaptic nerve terminals, we measured the paired-pulse ratio (PPR) evoked by 2 identical pulses (50 ms interpulse interval), and found that PPR was increased ($33.4{\pm}5.2$%) by 5-HT, reflecting decreased neurotransmitter releasing probability. These results suggest that 5-HT may decrease neurotransmitter release probability of glutamatergic corticostriatal synapse and may be able to selectively decrease non-NMDA glutamate receptor-mediated synaptic transmission.

• Journal of the Microelectronics and Packaging Society
• /
• v.17 no.2
• /
• pp.63-67
• /
• 2010

The organic solar cells with Glass/ITO/PEDOT:PSS/P3HT:PCBM/Al structure were fabricated using regioregular poly (3-hexylthiophene) (P3HT) polymer:(6,6)- phenyl $C_{61}$-butyric acid methyl ester (PCBM) fullerene polymer as the bulk hetero-junction layer. The P3HT and PCBM as the electron donor and acceptor materials were spin casted on the indium tin oxide (ITO) coated glass substrates. The optimum mixing concentration ratio of photovoltaic layer was found to be P3HT:PCBM = 4:4 in wt%, indicating that the short circuit current density ($J_{SC}$), open circuit voltage ($V_{OC}$), fill factor (FF) and power conversion efficiency (PCE) values were about 4.7 $mA/cm^2$, 0.48 V, 43.1% and 0.97%, respectively. To investigate the effects of the post annealing treatment, as prepared organic solar cells were post annealed at the treatment time range from 5min to 20min at $150^{\circ}C$. $J_{SC}$ and $V_{OC}$ increased with increasing the post annealing time from 5min to 15min, which may be originated from the improvement of the light absorption coefficient of P3HT and improved ohmic contact between photo voltaic layer and Al electrode. The maximum $J_{SC},\;V_{OC}$, FF and PCE values of organic solar cell, which was post annealed for 15min at $150^{\circ}C$, were found to be about 7.8 $mA/cm^2$, 0.55 V, 47% and 2.0%, respectively.

• Animal Bioscience
• /
• v.35 no.6
• /
• pp.892-901
• /
• 2022

Objective: This study was performed to investigate the potential effect of wheat phytase on long-chain inorganic polyphosphate (polyP)-mediated interleukin 8 (IL-8) signaling in an intestinal epithelial cell line, HT-29 cells. Methods: Cell viability and the release of the pro-inflammatory cytokine IL-8 in HT-29 cells exposed to polyP1150 (average of 1,150 phosphate residues) treated with or without wheat phytase were measured by the EZ-CYTOX kit and the IL-8 ELISA kit, respectively. Also, the activation of cellular inflammatory factors NF-κB and MAPK (p38 and ERK 1/2) in HT-29 cells was investigated using ELISA kits. Results: PolyP1150 negatively affected the viability of HT-29 cells in a dose-dependent manner. However, 100 mM polyP1150 dephosphorylated by wheat phytase increased cell viability by 1.4-fold over that of the intact substrate. Moreover, the 24 h exposure of cells to enzyme-treated 50 mM polyP1150 reduced the secretion of IL-8 and the activation of NF-κB by 9% and 19%, respectively, compared to the intact substrate. PolyP1150 (25 and 50 mM) dephosphorylated by the enzyme induced the activation of p38 MAPK via phosphorylation to 2.3 and 1.4-fold, respectively, compared to intact substrate, even though it had little effect on the expression of ERK 1/2 via phosphorylation. Conclusion: Wheat phytase could attenuate polyP1150-induced IL-8 release in HT-29 cells through NF-κB, independent of MAP kinases p38 and ERK. Thus, wheat phytase may alleviate inflammatory responses including hypercytokinemia caused by bacterial polyP infection in animals. Therefore, wheat phytase has the potential as an anti-inflammatory therapeutic supplement in animal husbandry.

• Korean Journal of Acupuncture
• /
• v.34 no.4
• /
• pp.185-190
• /
• 2017

Objectives : The research was conducted for the use of HT1 in the shoulder disease through correct acupuncture. Methods : (1) The contents were compared through reviewing literature. (2) The literature was studied in conjunction with the shoulder pathology and anatomical structures. Results : (1) The location of HT1 is described as 'in the axilla, over the axillary artery' in many literature, and the depth of HT1 is as shallow as 1 cm. The WHO standard also follows this. (2) There were many references to 'in the axilla, between the big muscles' in later generations, but there were mixed opinions about the exact muscle names. Based on the acupunctural review and the musculoskeletal study, the big muscles are considered to be 'Pectoralis major' and 'Latissimus dorsi'. (3) Among the muscles constituting the rotator cuff, applying acupuncture on HT1 is essential for 'Subscapularis m.'. Therefore, it is effective to stimulate 'Subscapularis m.' to a depth of 1.5 to 4 cm. Conclusions : The accurate acupoint of HT1 enables various uses of flank, armpit, shoulder and arm diseases as well as cardiopathy. Especially, it helps to treat the muscles through safe and effective acupuncture in shoulder rotator cuff disorder.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2010.02a
• /
• pp.278-278
• /
• 2010

3.37 eV의 와이드 밴드갭과 60 mV의 높은 엑시톤 결합에너지를 갖는 반도체인 ZnO는 화학 및 열적 안정성, 압전특성 등 다양한 특성을 갖는 물질로써, 수열합성법을 이용하여 길이 $1.5{\mu}m$, 직경 100nm의 n-type ZnO 나노와이어를 성장시켰으며, P3HT는 유기 태양전지에서 가장 많이 사용되는 고분자 도너로써 열처리를 통하여 결정화 됨에 따라, 엑시톤의 확산속도나 전하의 이동도가 증가하여 더 많은 광전류를 생성하는 장점을 가지고 있다. 본 연구에서는 ZnO 필름이 아닌 n-type ZnO 나노와이어와 Poly(3-hexylthiophene) (P3HT)를 사용 하여 ZnO/P3HT 이종접합 태양전지를 제작하였다. 기판으로 글래스, 전극으로 ITO (Indium Tin Oxide), 나노와이어의 씨앗층으로 ZnO:Al를 스퍼터로 100nm 증착 하였다. Znc nitrate hydrate와 hexamethylenetetramine이 혼합된 수용액에서 기판을 담그고 n-type ZnO 나노와이어 성장 시키고, P3HT의 스핀 코팅조건과 열처리 온도를 변화시켜 P3HT의 두께와 결정화도가 ZnO/P3HT 이종접합 태양전지에 미치는 영향을 비교 분석 하였다.

• The Korean Journal of Physiology and Pharmacology
• /
• v.4 no.5
• /
• pp.355-359
• /
• 2000

Cocaine induces immediate early gene expression and behavioral changes by blocking dopamine transporters in the terminals of nigrostriatal neurons in the striatum. The pharmacological role of serotonin 2/1C (5HT2/1C) receptors in cocaine-induced expression of zif268 (NGFI-A, egr1 and Krox-24) mRNA, a member of the zinc finger, was investigated using quantitative in situ hybridization histochemistry in vivo. Behavioral alterations induced by cocaine were also monitored in relation with blockade of the receptors. Systemic injection of ritanserin (1 mg/kg, s.c.), a 5HT2/1C receptor antagonist, did not reverse behavioral alterations and zif268 mRNA gene expression induced by 15 mg/kg cocaine, i.p., in the dorsal and ventral striatum. These data indicate that ritanserin-sensitive 5HT2/1C receptors are not necessary for cocaine-induced behavioral alterations and zif268 mRNA gene expression in the striatum.

• Journal of Nutrition and Health
• /
• v.33 no.6
• /
• pp.660-667
• /
• 2000

Previous studies on the effect of age on intestinal taurine transport in animals have invariably shown a decline in the activity of the transport system with increasing age. In the present study changes in taurine transporter activity were observed during cell differentiation in the human colon carcinoma cell line HT-29 This cell line exhibits various enterocytic characteristics when differentiated and therefore has frequently been used to study the characteristcs and regulation of nutrient and drug absorption in the small intestine at the cellular level. Pre-treatment of the cells with $\beta$-alanine(10mM) reduced the taurine transport activity to 33% of the value for the control cells(p<0.05) which implies that taurine and $\beta$-alanine share a common $\beta$-amino acid transport system for their celluar uptake in the HI-29 was continued until 21 days post seeding. Kinetic studies of the taurine transporter were conducted in the HT-29 cell line with varying taurine concentration(5-60$\mu$M) in the uptake medium Both Vmax and the Michaelis-Menten constant(Km) of taurine transporter were decreased as differentiation of the HT-29 cell line was progressed ; Vmax of the taurine transporter in cells incubated for 4, 14 and 21 days post seeding was 2.79$\pm$3.4m 16.89$\pm$1.74, and 0.85$\pm$0.08 and 0.32$\pm$0.01nmol.mg protein-1 .30min-1 respectively(p<0.001) and Km was 42.3$\pm$3.4, 16.89$\pm$1.74, and 11.2$\pm$3.0$\mu$M respectively (p<0.01) These results indicate that the activity of sodium dependent active taurine transport system in the HT-29 cell line is decreased as confluent cells are differentiated. This phenomenon in cell culture system corresponds well with the earlier observation of lower intestinal taurine transport activity in suckling rats compared to that in adult animals although direct relationship of cell differentiation with in vivo aging process needs further verification.

• Journal of Radiation Protection and Research
• /
• v.22 no.2
• /
• pp.97-101
• /
• 1997

During the period of March 1995 to December 1995, tritium concentrations of tritiated water vapor (HTO) and tritiated hydrogen (HT) in the atmosphere in Taejon were measured to evaluate present background levels of tritium in the atmosphere. Air samples were collected continuously for three weeks with a sampling system for tritium in the atmosphere and were analyzed by a liquid scintillation counting system. The range of the atmospheric HTO concentrations was 3.2-36 mBq $m^{-3}$ with a mean value of 16.2 mBq $m^{-3}$. The atmospheric HTO concentrations were the highest in summer and the lowest in winter. This trend was similar to the variation of atmospheric absolute humidity. The specific activities of tritium in atmospheric water vapor in Taejon ranged from 0.62 Bq $L^{-1}$ to 3.82 Bq $L^{-1}$ with a mean value of 2.04 Bq $L^{-1}$. The atmospheric HT concentrations were in the range of 35.7 mBq $m^{-3}$ to 48.9 mBq $m^{-3}$ with a mean value of 41.1 mBq $m^{-3}$.