• 대한약침학회지
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• 제11권1호
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• pp.149-162
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• 2008

Objectives : The purpose of this study is to investigate the effects of hot pepper extract and capsaicin on the adipogenesis in 3T3-L1 cells, lipolysis in rat epididymal adipocytes and histological changes in porcine adipose tissue. Methods : Inhibiton of preadipocyte differentiation and/or stimulation of lipolysis play important roles in reducing obesity. 3T3-L1 preadipocytes were differentiated with adipogenic reagents by incubating for 3 days in the absence or presence of hot pepper extract or capsaicin ranging from 0.01 to $1mg/m{\ell}$. The effects of hot pepper extract and capsaicin on adipogenesis were examined by measuring GPDH activity and by Oil Red O staining. Mature adipocytes from rat epididymal fat pad was incubated with hot pepper extract or capsaicin ranging from 0.01 to $1mg/m{\ell}$ for 3 hrs. The effects of hot pepper extract and capsaicin on lipolysis were examined by measuring free glycerol released. Fat tissue from pig skin was injected with hot pepper extract or capsaicinCFP ranging from 0.1 to $10mg/m{\ell}$ to examine the effects of hot pepper extract and capsaicin on histological changes under light microscopy. Results : The following results were obtained from present study on adipogenesis of preadipocytes, lipolysis of adipocytes and histological changes in fat tissue. 1. Hot pepper extract and capsaicin inhibited adipogenic differentiation at the concentration of 0.1 and $0.01mg/m{\ell}$, respectively, indicating that capsaicin was more effective in inhibiting adipogenesis than hot pepper extract. 2. Hot pepper extract and capsaicin decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) at the concentration of 0.1 and $0.01mg/m{\ell}$, respectively, indicating that capsaicin was more effective in inhibiting adipogenic differentiation than hot pepper extract. 3. Hot pepper extract and capsaicin increased glycerol release at the concentration of $0.1mg/m{\ell}$. There was no difference in lipolytic activity between hot pepper extract and capsaicin at the corresponding concentration. 4. Hot pepper extract and capsaicin caused shrinkage of fat cells, resulting in cell death at the concentration of $1.0mg/m{\ell}$, although capsaicin exerted this action over wide area than hot pepper extract. Conclusions : These results suggest that hot pepper extract and capsaicin efficiently inhibited adipogenesis, increased lipolysis of adipocytes and caused to shrink fat cells. Future studies are needed to make use of hot pepper extract pharmacopuncture for the treatment of obesity.

• 대한약침학회지
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• 제11권1호
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• pp.109-118
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• 2008

Objective : The objective of this study was to compare the antioxidant effects of hot pepper extract and capsaicin. Methods : In vitro, antioxidant activities were examined by DPPH radical scavenging activity, total antioxidant capacity(TAC), oxygen radical scavenging capacity(ORAC), inhibition of induced lipid peroxidation using liver mitochonria and total phenolic contents. Results : 1. DPPH free radical scavenging activities at the concentrations of both 1 and $10mg/m{\ell}$ were 1.2 to 1.9 times higher in capsaicin than in hot pepper extract. The concentration of capsaicin required for 50% radical scavenging was lower than that of hot pepper extract(3.9 vs $5.9mg/m{\ell}$), indicating that capsaicin had higher DPPH radical scavenging activity than hot pepper extract. 2. Total antioxidant capacities of capsaicin at the concentrations of 0.1 and 1mg/ml(13.8 and 41.3 nmol Trolox equivalent) were not significantly different from those at the concentrations of 1 and $10mg/m{\ell}$(11.4 and 41.2nmol Trolox equivalent), indicating that capsaicin showed 10 times higher ABTS radical scavenging activity compared to hot pepper extract. 3. ORAC of capsaicin at the concentrations of 1, 5, 10 and 100 mg/ml were 0.04, 0.17, 0.29 and 1.74nmol gallic acid equivalent, respectively. On the other hand, ORAC of hot pepper extract at the concentrations of 1, 5, 10 and $100{\mu}g/m{\ell}$ were 0.15, 0.44, 0.75 and 2.49nmol gallic acid equivalent, respectively, indicating that capsaicin showed higher peroxyl radical scavenging activity than hot pepper extract. 4. Inhibition of lipid peroxidation caused by hot pepper extract at the concentrations of 1 and $10mg/m{\ell}$ were 12.2 and 61.4%, respectively. Inhibition of lipid peroxidation caused by capsaicin at the concentrations of 1 and $10mg/m{\ell}l$ were 64.0 and 96.8%, respectively. Thus capsaicin showed 10 times stronger effect in inhibiton of lipid peroxidation than hot pepper extract. 5. Total phenolic contents of hot pepper extract at the concentrations of 0.1 and $1mg/m{\ell}$ were 1.4 and 20.8nmol gallic acid equivalent, respectively. Total phenolic contents of capsaicin at the concentrations of 0.1 and $1mg/m{\ell}$ were 6.1 and 55.4 nmol gallic acid equivalent, respectively, indicating that capsaicin had 2.7 to 4.3 times higher total phenolic contents than hot pepper extract. Conclusions : In summary, the results of this study demonstrate significant antioxidant activity of hot pepper extract, although the activity was lowered compared to capsaicin, suggesting that hot pepper extract play a role in prevention of oxidative-related diseases.

• 한국식품조리과학회지
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• 제24권6호
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• pp.882-890
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• 2008

The present study investigated the influence of different volumes of red hot pepper juice on the quality characteristics of fresh Pasta noodle. Supplementation with 0% (control), 2.5%, 5%, 7.5%, or 10% red hot pepper juice produced similar gelatinization characteristics of peak viscosity, temperature at peak viscosity, hot paste viscosity and numerical value of breakdown. However, increasing concentrations of red hot pepper juice produced progressively and significantly low cold paste viscosity and setback. The chromaticity of wet and cooked noodles was significantly lower in L value and significantly higher in +a and +b values with increasing volumes of red hot pepper juice. The texture of fresh noodles displayed no significant differences in hardness, adhesiveness and chewiness. The springiness and cohesiveness were lower and higher with the increase of added red hot pepper juice, respectively, but the differences just attained significance. For cooked noodles, adhesiveness, springiness, cohesiveness and chewiness tended to be higher with increasing volumes of red hot pepper juice, but again the differences just attained significance. Cooking characteristics of weight, volume, moisture absorptive power and turbidity decreased with increasing volumes of red hot pepper juice. Sensory characteristics of acceptability including appearance, color, flavor, taste, texture and overall-acceptability improved with increasing red hot pepper juice volume, in particular with 5% and 7.5%. Amylograph characteristics for initial paste temperature positively correlated with the texture characteristics for chewiness (p<0.05). Negatively correlated amylograph parameters included texture for springiness with for peak viscosity (p<0.01), texture for adhesiveness with hot paste viscosity (p<0.01) and breakdown with texture for adhesiveness, cohesiveness and chewiness (p<0.05).

• 한국가금학회지
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• 제25권1호
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• pp.21-30
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• 1998

The effects of dietary levels and feeding period of Korean hot pepper (Capsicum annum) seed on the performance and egg quality were investigated. Rhode Island Red layers of 84 wk of age were fed the experimental diets containing O.O(Control), 0.5, 1.0, 2.0, and 3.0% of HPS, respectively, in individual cages during 10 wk of the feeding period. The dietary hot pepper seed significantly(P<0.05) improved hen-day egg production and daily egg rnass, but reduced egg weight(P<0.05). During the 10 wk of the feeding period, the egg production and daily egg mass improved after 6 or 7 wk of feeding 0.5, 1.0 or 2.0% hot pepper seed diets. When the layers were fed the 3.0% HPS diet, it only took 1 wk to improve the egg production and daily egg mass. In addition, the birds fed 3.0% hot pepper seed diet showed relatively constant egg weight while those of the Control increased gradually as the feeding period passed. The dietary hot pepper seed significantly improved the eggshell thickness, and redness and whole color of egg yolk. The results indicate that dietary hot pepper seed( $\geq$2.0%) may improve the egg production and egg shell thickness, and increase the redness of egg yolk in layers.

• 생물환경조절학회지
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• 제27권4호
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• pp.356-362
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• 2018

고추는 조미채소의 한 종류로 우리나라 채소시장에서 큰 비중을 차지하는 품목이다. 대부분 고추의 이용은 건고추의 분말형태인 고춧가루로 가공하여 이용되고 있으므로, 고추건조에 대한 품질과 생산성 향상이 중요한 부분이다. 따라서 본 연구에서는 고추의 건조방법에 따라 고춧가루의 품질을 좌우할 수 있는 성분의 변화에 대하여 비교하고, 생산비를 고려하여 적정 건조방법을 제시하고자 실험을 수행하였다. 건조방법으로는 관행으로 많이 이용되는 양건과 화건을 이용하였으며, 제습기를 이용한 제습건조방식을 추가 적용하여 건고추 생산성과 품질을 비교하였다. 고추의 건조방식에 따른 건조율은 화건이 85.1%로 제일 높았고, 이에 따라 고춧가루의 수분함량도 13.5%로 가장 낮게 나타났다. 고추의 색택을 좌우하는 ASTA(American Spice Trade Association) 색도는 양건처리에 비해 화건과 제습건조처리가 높은 결과를 보였다. 고춧가루의 신미성분인 capsaicinoid 함량은 양건처리에 비하여 화건과 제습건조처리에서 모두 낮게 나타나 이에 대한 추가적인 적정 건조조건에 대한 연구가 추가되어야 된다. 당함량은 건조시간이 비교적 짧았던 화건과 제습건조처리에서 양건에 비해 높게 나타났으나 두 처리간에는 유의한 차이를 보이지 않았다. 건고추 생산비용은 제습건조처리가 화건처리에 비해 9.9% 효율이 높은 것으로 나타났다. 본 연구를 통해 화건과 제습건조가 당함량의 증진과 건고추의 색 발현 측면에서는 효과적 이었음을 알 수 있었다. 고추의 신미성분인 capsaicinoid 함량 증진을 위해서는 화건과 제습건조처리에서 적정한 건조온도와 건조시간에 대한 연구가 추가되어야 될 것으로 판단되었다.

• Journal of Nutrition and Health
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• 제2권2호
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• pp.99-103
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• 1969

A method of assaying Capsaicin in hot pepper Paste, which is the most Characteristic Korean food, was composed by making of Azodye method. Capsaicin, a hot ingredient of hot pepper. was isolated from the acetone-ether(1:3) extract of hot pepper paste with Thin layer Chromatography and analyzed quantitatively with Spectrophotometry.

• 한국식품영양과학회지
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• 제24권6호
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• pp.837-842
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• 1995

고추가루 첨가 식이가 체액성 면역기능에 미치는 영향을 조사하였다. 실험동무로 흰쥐를 사용하여 대조군과 2%, 5%, 10% 고추가루 첨가 식이군을 설정한 후 27일간 실험식이로 사육하였다. 실험종료 10일 전에 각군의 흰쥐를 면양적혈구로 세차례 면역시킨 후 항체생성 세포수 및 혈청 중 항체 농도 등 체액성 면역기능을 중심으로 조사하였다. 그 결과, 대조군에 비해 고추가루 첨가 식이군의 항체 생성 세포수, 적혈구 응집반응 및 혈청중 항체 농도가 높은 것으로 나타났으며, 또한 이때 고추가루 첨가식이군의 각 장기조직 중 AsA 함량은 대조군에 비해 높은 것으로 나타났다. 이들 결과로 보아, 고추성분은 체액성 면역기능의 활성화에 기여하는 물질을 함유하고 있을 뿐만이 아니라, AsA의 공급효과가 뛰어난 것이 확인되었다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.80.2-81
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• 2003

Since hot peppers (Capsicum annuum L.) are getting reputation as an important source of vitamins, medicine and many other areas, consumption and cultivation is being increased in the world. In spite of this usefulness, so little attention has been given to the hot pepper plants. To date, less than 500 nucleotide sequences including redundancy has been identified in NCBI database. Therefore we started to EST sequencing project for initial characterization of the genome, because of the large genome size of hot pepper (2.7 3.3 ${\times}$ 109 bp), To date, a set of 10,000 non-redundant genes were identified by EST sequencing for microarray-based gene expression studies. At present, cDNA microarrays containing 4,685 unigene clones are used for hybridization labeled targets derived from pathogen infected and uninoculated leaf tissues. Monitoring of gene expression profiles of hot pepper interactions with soybean pustule pathogen (Xag；Xanthomonas axonopodis pv. glycine) will be presented.

• 한국축산식품학회지
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• 제38권3호
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• pp.451-467
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• 2018

Hot pepper has anti-obesity effects by controlling appetite and reducing blood fat level. To reduce the pungency of capsaicin, red or green hot pepper juice was fermented with Bacillus licheniformis SK1230. Fermented hot pepper juice was then added into yogurt at different ratios. The pH of yogurt added with hot pepper juice was decreased from 4.61 to 4.48. Titratable acidity and counts of lactic acid bacteria were increased with increasing amount of pepper juice added. However, the viscosity was decreased significantly compared to the control. On chromaticity test, when more pepper juice was added, $L^*-value$ was decreased whereas $a^*$- and $b^*$- values were increased significantly (p<0.05). The spectrum of antimicrobial activity of yogurt was slightly changed compared to using pepper juice. Total polyphenol contents and antioxidant activity were increased with increasing amount of pepper juice added. Stirred yogurt added with fermented red pepper juice at 3% or green pepper juice at 1% showed high scores in flavor, appearance, texture, and overall acceptance in sensory test. Yogurt added with fermented pepper juice with reduced pungency showed also good palatability during storage at $4^{\circ}C$. Yogurt with added hot pepper juice can be play an important role in functional food relative to anti-obesity.

• 한국식품영양과학회지
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• 제21권1호
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• pp.64-69
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• 1992

Blanching 처리가 고추의 건조율 및 색도에 미치는 효과를 조사하고자 실험실 규모의 열풍 건조기를 제작하였으며 strain gage형의 load cell과 strain amplifier를 이용하여 건조 중의 중량을 연속적으로 기록, 측정하였다. 고추의 건조특성곡선은 대조군에서는 예비, 항율, 감율건조기간이 뚜렷한 반면, blanching 처리군은 감율건조기간만 존재하였으며, 시료의 건조율과 건조상수 값은 blanching 처리 온도와 시간에 비례하여 증가하는 경향이었고, $100^{\circ}C$, 3분 blanching 처리군이 가장 높게 나타났다. Capsanthin 함량 역시 대조군에 비해 처리군이blanching 처리온도와 시간에 비례하였으며 색도 값의 경우 $80^{\circ}C$, 3분 blanching군이 가장 우수하였다. blanching 처리는 전반적으로 고추의 건조율 증진 및 색도 보존에 효과적이며 $80^{\circ}C$, 3분 blanching 처리가 비교적 적합하였다.