• Journal of Nutrition and Health
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• v.36 no.1
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• pp.18-23
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• 2003

We studied the effects of hot water extract of Inonotus obliquos mushroom on the proliferation and apoptosis of the human colon adenocarcinoma, HT-29 and the human stomach adenocarcinoma, SNU-484 cell. Cells were maintained with Dulbecco's modified Eagle medium/Ham's F-12 nutrient mixture supplemented with 10% fetal bovine serum at 37$^{\circ}C$ in a humidified $CO_2$. For the cell proliferation experiments, cells were seeded in 35 mm dishes, and were treated with the various concentrations of the extract for the different time course. Apoptosis was measured by caspase-3 activity. When we incubated HT-29 cells for 24, 48, 72, and 96 hours after treatments, the cell proliferation was more suppressed with more treatment time. In case of the human stomach cancer cell, SNU484, the extract significantly decreased the cell number. Thus, the treatment of 1.5 mg/$m\ell$ extract decreased almost half of the cell number. Caspase-3 activity in HT-29 was increased by the treatment of mushroom extracts. In SNU484, caspase-3 activity tended to increase in proportion to the amounts of the extracts and the treatment of Inonotus obliquos affected the activity a lot. Therefore, Inonotus obliquos is suggested for the prevention of gastro-intestinal cancer and strongly recommended for the treatment of stomach cancer. (Korean J Nutrition 36(1) : 18~23, 2003)

• Korean Journal of Food Science and Technology
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• v.30 no.2
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• pp.391-396
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• 1998

To inhibit the import of hot sauce, the manufacturing process of the Korean type hot sauce was developed. Chemical analysis of the imported hot sauce showed as follows; moisture 95.66% (w/w), salt 2.79% (w/w), titratable acidity 3.62% (w/w), crude fiber 0.42% (w/w), reducing sugar 0.03% (w/w), capsanthin 0.89% (w/w), capsaicin 4.28 mg% and pH 3.26. Korean type hot sauce prepared with red pepper, salt and vinegar was aged in oak barrel for 12 months at $10^{\circ}C$. Chemical analysis of the Korean type hot sauce showed as follows; moisture 92.05% (w/w), salt 8.82% (w/w), titratable acidity 3.88% (w/w), crude fiber 3.14% (w/w), reducing sugar 1.78% (w/w), capsanthin 0.13% (w/w), capsaicin 2.32 mg% and pH 3.29. Among chemical components, the contents of reducing sugar, salt and crude fiber except capsaicin and capsanthin were more than those of imported one. Chemical components of the Korean type hot sauce were changed slightly during aging for 12 months. The cell counts of yeast were increased from $3.2{\times}10^2\;to\;5.2{\times}10^6$ till the second month and those of lactic acid bacteria were increased from $3.{\times}10^1\;to\;1.5{\times}10^7$ till the third month, those of yeast and lactic acid bacteria decreased from the eighth month showed $6.6{\times}10^4$ and not detectable on the twelfth month, respectively. As the results of sensory evaluation on the Korean type hot sauce were evaluated to be superior to the imported to be superior to the imported one.

• Journal of Institute of Control, Robotics and Systems
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• v.13 no.10
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• pp.940-948
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• 2007

The Advanced Spent Fuel Conditioning Process(ACP), which is the process of the reduction of uranium oxide by lithium metal in a high temperature molten salt bath for spent fuel, was developed at Korea Atomic Energy Research Institute (KAERI). Since the ACP equipment is located in an intense radiation field (hot cell) as well as in a high temperature, it must be remotely operated and maintained. The ACP hot cell is very narrow so the workspace of the wall-mounted mechanical Master-Slave Manipulators(MSMs) is restricted. A Bridge Transported Servo Manipulator(BTSM) system has been developed to overcome the limitation of an access that is a drawback of the mechanical MSMs. The BTSM system consists ot a bridge crane with telescoping tubeset, a slave manipulator, a master manipulator, and a control system. We applied a bilateral position-position control scheme with friction compensation as force-reflecting controller. In this paper, the transmission characteristics on the tendon-and-pulley train is numerically formulated and analyzed. Also, we evaluate the performance of the force-reflecting servo manipulator.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2004.06a
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• pp.278-284
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• 2004

The processes for the smelting of a metal product and the solidification of a molten salt were developed respectively to treat the products from the electrochemical reduction process. The method for the separation of a metal product in a magnesia container from the residual. salt and consequent smelting of it to a metal ingot by the multi step heating in vacuum was proposed. The new concept using a dual vessel and a salt valve was also suggested for the solidification of a molten salt into a regular size and shape which is suitable for the transport and measurement. The results obtained in the study will be applied to the design of the hot cell demonstration system of the Advanced Spent Fuel Conditioning Process of KAERI.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1715-1718
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• 2005

During demonstrations of a process conditioning spent nuclear fuels, it may be necessary to transport and handle Spent fuel road cuts from Post Irradiation Examination facility to Slitting device in The hot cell. It may be not easy to transport spent fuel rod cuts because rod cuts are high radioactive materials. For this purpose, we have developed a capsule for transporting and handling high radioactive materials. We have analyzed conditions of a hot cell and requirements of the device, designed and manufactured The prototype of the device, and done some performance tests. From the tests, it has been shown that transportation and handling without scattering nuclear material was smooth but the weight of capsule was heavy. These result will be reflected to a design of the improved transportation and handling device which will be used during demonstrations.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.684-687
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• 2002

The authors have developed two devices which cuts the spend fuel rod to an optimal size and extracts fuel pellet from the pieces of cut fuel rods. These devices are so important to reduce radioactive wastes that some advanced countries developed their own methods and devices. The authors have benchmarked from these methods and devices. For spent fuel rod cutting, the tube cutting method has been chosen. some mechanical properties of the fuel tube and pellet has been carefully considered for an optimal cutting size. For fuel pellet extraction, a mechanically extracting method has been adopted. The existing chemical method have turned out to be inappropriate because it produced large amount of radioactive wastes, in spite of its high fuel recovery characteristics. The developed method has an advantage that it can be applied to other fuel rods that have different shapes and sizes. The two devices are set up and operated in the hot cell where people can not go in, so that the devices have been designed to be controlled remotely and modulated for easy maintenance. And the performance of the devices has been tested by using simulated fuel rod. From the experimental results, the devices are supposed to be useful for reducing radioactive wastes.

• Journal of the Korean Home Economics Association
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• v.39 no.4
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• pp.49-59
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• 2001

Natural red and yellow dyestuff was extracted from safflower (Carthamus tinctorius Linnaeus) by a new process of cellulase extraction compared with the conventional hot water extraction. Dyestuffs were extracted from safflower easily and repeatedly by means of cellulose as safflower cell wall destroyer. It means that new dyestuff extraction by cellulase improves not only yields of dyestuff from safflower successfully but also the rate of repetition of extraction. From the above experiments, the conclusions of this study were summarized as follows. 1. The optimum conditions of dyestuff extraction from safflower by general extraction method were that the solvent was the water of pH 6.0 on yellow dyestuff and 3% $K_2CO_3$ solution on red dyestuff, extraction temperature was $55^{\circ}C$, and extraction time was 30 min. 2. Among various cellulase, the NOVO cellulase was the best cell wall destroyer of safflower and finally produced the largest amount of dyestuff from safflower by cellulase extraction method. 3. The optimum conditions of dyestuff extraction by cellulase extraction method were conducted on 10 unit of cellulase per gram of safflower at $100m{\ell}$ water of pH 5.0 at $50^{\circ}C$ for 30 min.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.5
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• pp.356-361
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• 2004

When SiHa cells were incubated for varying periods of time with extracts of PFF and PFM, the cytotoxicity of the ethanol extracts of PFF was higher than those of the other extracts. These results indicated that the extracts from fruiting bodies of p. ferulae contain antitumor Substances. When A549, SiHa and HeLa cells were incubated with different concentrations of PFF and PFM extracts, the ethanol extracts of PFF showed strong cytotoxicity against A549 tells at concentrations over $10{\mu}g/mL$ and against SiHa and HeLa cells at concentrations over $40{\mu}g/mL$. However, the differences in the cytotoxic effects of the hot water and ethanol extracts of PFM and the hot water extracts of PFF on all 3 cancer cells were not significant. Also, the PFF ethanol extracts induced synergistic effects on the TRAIL-induced apoptosis in A549 cells, which were strongly resistant to TRAIL. These results indicated that ethanol extracts of PFF were the most prominent antitumor agents toward lung cancer cells (A549).

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.11a
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• pp.116-119
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• 2004

In this paper, we have improved a novel (ECB) mode using tilt angle in the unique condition by hot-plate equipment. The new control of tilt angle for nematic liquid crystal (NLC) with negative and positive dielectric anisotropy on the rubbed homeotropic polyimide (PI) using baking method by Hot-plate equipment was investigated. LC tilt angle decreased with increasing baking temperature and time. Especially, the low LC tilt angle of positive type NLC $({\Delta}n>0)$ on the rubbed homeotropic PI surface by increasing temperature and time was measured. The EO characteristics of the novel ECB mode using control of tilt angle on the homeotropic surface than that of conventional OCB cell can be improved. We suggest that the developed the Novel ECB cell using control of tilt angle on the homeotropic surface is a promising technique for the achievement of a fast response time and a high contrast ratio.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.10a
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• pp.519-522
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• 1997

In this study, the collision free path planning method of the articulated manipulator using sequential search is proposed. This method is to find the joint path of the manipulator with many degrees of freedom from the distal joint to the proximal one. To do this, the initial work space of the gantry manipulator, which is a remote maintenance equipment of the radioactive environment, is defined from the condition that the distal joint configuration is determined by the posture of maintenance. Then, 2-dimensional configuration space with the obstacle area is represented and the collision free path of manipulator is searched in the configuration space. And, this method is verified using the graphic simulation in virtual workcell for the spent fuel disassembling processes. The result of this study can be effectively used in implementing the maintenance processes for the hot cell equipment and enhance the reliability of the spent fuel management.