• 식물분류학회지
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• 제50권3호
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• pp.327-332
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• 2020

Microsatellite markers were developed as a tool for phylogeographic studies of Hosta capitata. We also assessed cross-amplification in species closely related to Hosta capitata. We produced 28 polymorphic microsatellite markers by mapping 300 bp paired-end reads obtained from Illumina MiSeq data of H. capitata. In H. capitata, the number of alleles per locus ranged from 1 to 13. Observed and expected heterozygosity ranged from 0.000 to 0.844 and 0.000 to 0.832, respectively. Additionally, 13 loci were successfully transferable to the related species of H. minor and H. venusta. These markers will provide a powerful genetic tool not only for elucidating the phylogeographic patterns of H. capitata populations but also for studying the genetic delimitation of H. capitata from its related species.

• 한국자원식물학회지
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• 제32권1호
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• pp.53-62
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• 2019

본 연구는 우리나라에 자생하고 있는 비비추속 식물 6종(일월비비추, 주걱비비추, 다도해비비추, 좀비비추, 한라비비추, 흑산도비비추)의 정단을 이용하여 대량증식과 품종개발 등을 위한 기내증식체계를 확립하고자 하였다. 정단은 0.5, 1.0, 2.0, 4.0 mg/L BA와 0.1, 0.5, 1.0, 2.0 mg/L TDZ를 0.1 mg/L NAA와 각각 혼용한 조건과 PGRs을 무첨가한 조건(control)의 MS배지에 배양하였다. 배양 8주 후에 embryogenic callus, somatic embryo, crown bud, 그리고 신초와 뿌리의 분화 및 생육, 생체중 등에 대하여 조사하였다. 6종의 비비추 식물 모두에서 control에 비하여 PGRs 처리구의 embryogenic callus와 somatic embryo 형성율, 다신초 분화율이 높았다. 분화된 신초의 개수는 일월비비추는 2.0 mg/L TDZ에서 5.4개, 주걱비비추와 다도해비비추는 1.0 mg/L TDZ에서 각각 3.3개, 5.8개, 좀비비추는 0.5mg/L BA에서 11.1개, 흑산도비비추는 0.5 mg/L TDZ에서 9.8개, 한라비비추는 1.0 mg/L BA, 0.1 mg/L TDZ에서 8.1개로 가장 많았다. Somatic embryo 형성에서는 다도해비비추와 흑산도비비추가 처리한 PGRs에 대해 효과적이었고, 일월비비추, 주걱비비추, 좀비비추, 한라비비추에서는 상대적으로 효과가 적었다. 4종의 자생 비비추(일월비비추, 주걱비비추, 다도해비비추, 흑산도비비추)에서 조사된 crown bud도 control에 비하여 PGRs 처리구에서 더많이 형성되었다. 주걱비비추는 cytokinin의 종류 및 농도와 상관없이 callus와 신초 분화에 큰 효과가 나타나지 않았지만, crown bud의 형성에는 TDZ에서 약간 증가하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.80-80
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• 2018

Hosta is a genus of the family Asparagaceae and distributed in East Asia. There are six Hosta species (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) native to Korea and among them, four species (H. minor, H. jonesii, H. venusta and H. yingeri) are endemic to the Korea peninsula. Hosta is generally propagated by seed, crown division or tissue culture. However, tissue culture is a more efficient method to mass proliferation, a new cultivar development and disease-free plantlet production in a limit time. Hence, we conducted this study to evaluate the influence of various plant growth regulators (PGRs) treatments on the induction of callus and somatic embryo of the six Hosta species. Leaf, petiole and root were used to select optimum tissue culture explants. Petiole explants thus only were used for callus induction and somatic embryogenesis with TDZ (0.1, 0.5 or 1.0mg/L) and NAA (0.1 or 0.5 mg/L) combinations. After 12 weeks of culture, the highest rate of somatic embryogenesis was achieved on modificated MS medium containing 1.0 mg/L TDZ and 0.1 mg/L NAA in H. capitata and H. minor (15.5%, respectively), 0.1 or 0.5 mg/L TDZ and 0.1 mg/L NAA in H. jonesii (22.2%), 1.0 mg/L TDZ and 0.5 mg/L NAA in H. yingeri (26.7%), and 0.1 mg/L TDZ and 0.5 mg/L NAA in H. venusta (53.3%). H. clausa showed very low effect on somatic embryogenesis by PGRs; 2.2%. There was interspecies difference to PGRs respond for callus and somatic embryo induction. Regenerated multiple shoots and plantlet of H. minor, H. jonesii, H. venusta and H. yingeri were obtained via somatic embryogenesis.

• Journal of Plant Biology
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• 제37권3호
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• pp.277-284
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• 1994

I investigated levels of genetic diversity, population genetic structure, and gene flow in Hostacapitata, a herbaceous perennial native to South Korea and southwestern Japan. Starch gel electrophoresis was conducted on leaves collected from 310 plants in 19 Korean populations. Twenty-two of 25 putative loci examined were polymorphic in at least one populatin and the mean number of alleles per locus was 1.65. In addition, mean expected heterozygosity within populations (Hep=0.153) was higher than average values for species with similar life history traits. Significant differences in allele frequency were detected between populations at all loci (P<0.01), and slightly over 30% of the genetic variation was found among populatins (GST=0.308). Indirect estimates of the number of migrants per generation (Nm) (0.506, calculated from GST; 0.852, calculated from the mean frequency of ten private alleles) indicate that gene flow is restricted among the isolated Korean populations of H. capitata. Factors contributing to the high levels of genetic differentiation among populations of H. capitata include small and discrete populations, human disturbance, and low frequencies of pollinator foraging behavior.

• 한국약용작물학회지
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• 제12권5호
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• pp.397-400
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• 2004

The chromosome numbers and karyotypes were investigated in four Korean native species of the genus Hosta. The chromosome complements were diploid of 2n=60 in H. japonica var. lancifolia Nakai and H. capitata Nakai, aneuploid of 2n=59 in H. minor (Bak.) Nakai, and modified triploid of 2n=92 in H. longipes (Fr. et Sav.) Matsumura. All the species carried four sets of distinctly large chromosomes of which the chromosome types were telocentrics or subtelocentrics with $4.4{\sim}7.2\;{\mu}m$ in length. The other chromosomes were meta-, submeta, subtelo-, or telocentric types and showed gradual length degradation in the range of $1.0{\sim}3.0\;{\mu}m$. The satellites appeared vestigially in a pair or a triplet set of chromosomes which depends on the species. New chromosome number and karyotype in H. longipes were the first report in this species. The structural rearrangement was suggested to explain the modified triploid composition of 2n=92.

• 환경생물
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• 제38권1호
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• pp.197-205
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• 2020

본 연구는 경관식재용 지피식물의 혼식 설계를 위하여 생활형이 다른 9종의 식물의 연중 생장 특성을 파악하고 식물들 간의 피복 특성을 분석하여 생활형에 따른 적정 식재조합을 도출하고자 본 연구를 실시하였다. 단립형으로 초장 20 cm 이상 생장하는 둥굴레, 맥문동, 일월비비추는 4월 식재 후 60일 이내 생육이 증가한 후 형태를 유지하였다가 9월 이후 지상부가 퇴화하며 휴면기에 들어가는 경향을 보였고, 분지형으로 20 cm 이하로 생장하는 바위취, 패랭이꽃, 애기기린초는 4월 식재 후 9월까지 지속적으로 생장하다가 9월 이후 생장이 감소하는 특성을 나타냈다. 포복형으로 지면에 붙어서 생장하는 리시마키아는 식재 후 3개월 경과부터 급속한 생장을 보였으며 돌나물은 8월까지 서서히 생장하다가 온도가 하강하는 9월부터 지상부가 퇴화하는 특성을 보였다. 꽃잔디는 초반 생장은 좋았으나 개화기가 지나고 난 후에는 전반적인 생육발달이 더뎌져 식재 후 7개월 동안 11.0%의 낮은 생장 특성을 보였다. 이와 같이 각기 다르게 생장하는 9종의 식물들을 혼식하였을 때 피복하는 특성을 조사한 결과 각 식물의 생장 특성이 피복에 영향을 미치는 것을 알 수 있었는데, 초장이 10~20 cm 자라며 로제트형으로 자라는 형태와 포복형 식물들의 조합이 높은 피복률을 나타냈다. 식물별로는 포복형인 돌나물이 맥문동, 일월비비추, 패랭이꽃, 애기기린초, 꽃잔디 등이 가장 많은 식물들과 혼식하였을 때 생장이 증가하는 종이었다. 로제트형 중에서는 일월비비추가 애기기린초, 바위취, 리시마키아, 돌나물과 동반생장이 가능한 종이었고, 지표면에서 분지가 밀생하여 자라는 패랭이꽃은 잎이 밀생하지 않는 맥문동, 바위취, 리시마키아, 돌나물과 혼식이 유리한 경향을 나타났다. 이 결과는 식물의 생장형 분류 중 근경이 분지하는 종보다 땅위로 뻗는 줄기로 번식하거나 무근경으로 영양 생장하는 종이 다른 종들과 혼식하기에 좋은 유형이라고 사료되었다.

• 한국조경학회지
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• 제21권1호
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• pp.63-82
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• 1993

In the middle areas of South Korea, we collected 308 wild herbaceous species for studying the possibility of landscaping and, growing them at West in Yongin country of Kyunggi province. We observed their vegetative and flowering characteristic. And the result is as follow: 1. 76 species(24.7%) such as Hosta capitata, etc. of them are thought useful for flower-bedding or landscaping for park and ground-covering, while 94 species(30.6%) are regarded useful for ground-covering, potting and indoor-planting. They are Nepatica maxima and Thaliticum minus var. hypoleucum, etc. 46 species(15%) seems to be useful for flower-bedding, ground-covering and potting. 2. 264(85.7%) of all the observed species are for the enjoyment of their flowers, while 36 species(11.4%) for the enjoyment of their leaves, and 9 species(2.9%) for their fruit. They are Viburnum dilatatum, etc. 3. As for the relation of their habits to light conditions, 148 species(48%) grow int he full sun of more than 50,000Lux, 138 species(44.8%) usually in in the half shade of 20,000 to 30,000Lux, 15 species(4.9%) in the shade of some 10,000Lux, and 7 speciese grow either in the full sun or in the shde. 4. some species such as Wasabia koreansa, Allium succuliferum, Asterglehni, Ligulariafisheri, Ligularia stenocephala Epimedium koreanum are considered available as edible plants as well as ornamentals plants, and Houttuynia cordata. Saururus chinensis, Epimedium koreanum need to be further developed not only as ornamental plants but also medicinal plants.

• 한국환경생태학회지
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• 제16권4호
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• pp.387-403
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• 2003

여원재에서 만복대 사이 백두대간에 해당되는 지역에 분포하는 식물상을 조사한 결과 104과 305속 439종 60변종 3품종 총 502종류로 확인되었다. 산림청이 지정한 회귀 및 멸종위기종은 구름송이풀, 구상나무 금마타리, 께묵, 눈향나무(식), 땅나리. 목련(식). 산작약. 뻐꾹나리. 금강제비꽃의 총 10종류와 환경부의 자연환경보전법에 의한 멸종위기 및 보호야생식물종은 보춘화 1종류가 조사되었다. 한국특산식물 종은 개나리(식), 구상나무, 금마타리, 꽃마리, 나비나물, 노각나무, 말채나무, 병꽃나무, 분취, 뻐꾹나리, 일월비비추, 할미밀망 등의 총 24종류가 확인되 었다.

• 한국환경복원기술학회지
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• 제11권4호
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• pp.37-45
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• 2008

The vascular plants of the studied area in Nonsan economical forest growing site appeared to be 341 taxa; 81 families, 218 genera, 290 species, 1 subspecies, 42 varieties and 9 forms. Based on the list of Korean endemic plants, 5 taxa existed; Cephalotaxus koreana, Thalictrum actaefolium, Asperula lasiantha, Populus tomentiglandulosa (Planted species), Paulownia coreana (Planted species). Specific plant species by floral region were total 13 taxa; Cirsium setidens in class III, Potentilla dickinsii in class II, Cephalotaxus koreana, Pinus koraiensis (Planted species), Hosta capitata, Juglans mandshurica, Alnus hirsuta, Quercus variabilis, Ulmus parvifolia, Dictamnus dasycarpus, Gwenia biloba var. parviflora, Vaccinium oldhami, Trigonotis icumae in class I. The naturalized plants in this site were 9 families, 19 genera, 20 species, 1 varieties, 21 taxa and naturalization rate was 6.1% of all 341 taxa vascular plants. The communities were classified into Quercus acutissima community, Pinus densiflora community, Quercus variabilis community, Platycarya strobilacea community, Quercus aliena community of natural forests and Pinus rigida community, Metasequoia glyptostroboides community, Larix leptolepis community of artificial forests.

• 한국환경과학회지
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• 제18권6호
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• pp.633-644
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• 2009

The vascular plants in the Gyeryongsan National Park were listed 227 taxa; 71 families, 148 genera, 196 species, 1 subspecies, 26 varieties and 4 forms, Among them, trees and herbs were 103 taxa (45.4%), 124 taxa (54.6%), respectively. The rare plants were identified 6 taxa such as Paeonia obovata (Legal protection species), Viola albida, Acanthopanax chiisanensis, Lilium distichum, Lilium callosum and Goodyera schlechtendaliana by the Korea Forest Service. Based on the list of Korean endemic plants, 8 taxa were recorded; Cephalotaxus koreana, Pseudostellaria multiflora, Thalictrum uchiyamai, Indigofera koreana, Acanthopanax chiisanensis, Asperula lasiantha, Weigela subsessilis and Carex okamotoi. Based on the list of approved for delivering overseas of plants, 6 taxa were recorded; Viola albida, Acanthopanax chiisanensis, Asperula lasiantha, Carex okamotoi, Lilium distichum and Lilium callosum. Specific plant species by floral region were total 21 taxa (9.3%); Paeonia obovata in class V, 5 taxa (Betula davurica, Acer palmatum, Asperula lasiantha, Lonicera subhispida, Phaenosperma globosa) in class III, 4 taxa (Viola orientalis, Acanthopanax chiisanensis, Ostericum melanotilingia, Lilium distichum) in class II and 11 taxa (Cephalotaxus koreana, Betula schmidtii, Quercus variabilis, Aphananthe aspera, Ilex macropoda, Meliosma oldhamii, Tilia amurensis, Lilium callosum, Erythronium japonicum, Polygonatum inflatum, Hosta capitata) in class I. It is important that relationship between national and local government will be needed for preservation of natural environment of Gyeryongsan National Park. As well, a long time of monitering and systematical management counterplan will be required.