• Title/Summary/Keyword: Host range

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Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea

  • Cho, Heejung;Song, Eun-Sung;Lee, Young Kee;Lee, Seungdon;Lee, Seon-Woo;Jo, Ara;Lee, Byoung-Moo;Kim, Jeong-Gu;Hwang, Ingyu
    • The Plant Pathology Journal
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    • v.34 no.1
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    • pp.23-34
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    • 2018
  • The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylogenetic relatedness based on the analysis of phylotype, biovar, and host range. Of the 93 isolates, twenty-two were identified as biovar 2, eight as biovar 3, and sixty-three as biovar 4. Applied to the phylotype scheme, biovar 3 and 4 isolates belonged to phylotype I, and biovar 2 isolates belonged to phylotype IV. This classification was consistent with phylogenetic trees based on 16S rRNA and egl gene sequences, in which biovar 3 and 4 isolates clustered to phylotype I, and biovar 2 isolates clustered to phylotype IV. Korean biovar 2 isolates were distinct from biovar 3 and 4 isolates pathologically as well as genetically - all biovar 2 isolates were nonpathogenic to peppers. Additionally, in host-determining assays, we found uncommon strains among biovar 2 of phylotype IV, which were the tomato-nonpathogenic strains. Since tomatoes are known to be highly susceptible to RSSC, to the best of our knowledge this is the first report of tomato-nonpathogenic potato strains. These results imply the potential prevalence of greater RSSC diversity in terms of host range than would be predicted based on phylogenetic analysis.

Host Range, Occurrence and Developmental Characteristics of Ceroplastes pseudoceriferus (Homoptera : Coccidae) on Persimmon Trees (감나무에 기생하는 뿔밀깍지벌레의 기주범위, 발생소장, 각 태별 생육 특성)

  • 박종대;박인선;김규진
    • Korean journal of applied entomology
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    • v.29 no.4
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    • pp.269-276
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    • 1990
  • This study investigated host range, seasonal occurrence and developmental characteristics of Ceroplastes pseudoceriferus Green on persimmon trees. The host plants covered 34 families and 66 species, including newly recorded species of Chenopodum album var. centrorubrum, Glycine max, Begonia semperflorens, Pharbitis nil, Perilla frutescens var. japonica and Erigeron canadensis. There was one generation of C. pseudoceriferus a year with larval stage expanding from mid-June to mid-October. Average number of eggs laid per female was 1,073$\pm$177.3. Hatching rate was 97.3% and was not affected by temperatures and photoperiods. Stadium varied as 23.4 days in eggs, 128.3 days in larvae and 213.3 days in female adults including overwintering periods at natural condition.

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A Mobile Multicasting Mechanism Based on Mobility Information of Mobile Hosts (호스트의 이동 정보에 근거한 모바일 멀티캐스팅 기법)

  • Baek DeukHwa;Kim Jaesoo
    • Journal of Korea Multimedia Society
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    • v.8 no.2
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    • pp.258-268
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    • 2005
  • The efficient provision of multicast service to moving hosts in mobile computing environments is not so easy task. Bi-directional tunneling scheme causes overhead about encapsulation and triangular routing. On the other hand, remote subscription scheme need freDuent tree reconstruction, which is inefficient for rapid moving hosts. In this paper we propose Mobility Based Mobile Multicast(MBMOM) scheme which is based on host's mobility information. Ultimately MBMOM try to find the strong points of remote subscription scheme and hi-directional tunneling scheme. If host's mobility speed is considered to be high, multicast packets are forwarded using hi-directional tunneling scheme from home agent continuously. If host's mobility speed is considered to be slow, remote subscription scheme is applied for foreign agent and it try to join multicast tree. We developed analytical models to analyze the performance of proposed scheme and simulated our scheme compared with MOM(Mobile Multicast), RBMOM(Range Based MOM), and TBMOM(Timer Based MOM) schemes. Simulation results show that our scheme has shorter transmission delay than above 3 schemes in the aspect of host's mobility speed and multicast group size.

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Ecological Factors Influencing Severity of Cashew Fusarium Wilt Disease in Tanzania

  • Lilai, Stanslaus A.;Kapinga, Fortunus A.;Nene, Wilson A.;Mbasa, William V.;Tibuhwa, Donatha D.
    • Research in Plant Disease
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    • v.27 no.2
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    • pp.49-60
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    • 2021
  • Cashew (Anacardium occidentale L.) is an important cash crop in Tanzania as a source of income to cashew growers and provides foreign exchange for the country. Despite its significance, the crop is threatened by fast spreading disease known as cashew Fusarium wilt caused by Fusarium oxysporum. Field assessment and laboratory tests were conducted to determine incidences of the disease, severity, ecological factors that influence them and explored the pathogen host specificity in six cashew growing districts. The results revealed significant (P<0.001) variation of disease incidences and severity among the studied districts. The results further revealed that there is both positive and negative correlation between the incidence and severity of the disease versus the evaluated ecological factors. The soil pH, soil temperature, air temperature, and relative humidity depicted positive correlation of disease incidence and severity versus ecological factors at ρ=0.50 and ρ=0.60, ρ=0.20 and ρ=0.94, ρ=0.11 and ρ=0.812, ρ=0.05 and ρ=0.771 respectively while nitrogen, phosphorus, and carbon depicted negative correlations at ρ=-0.22 and ρ=-0.58, ρ=-0.15 and ρ=-0.94, ρ=-0.19 and ρ=-0.12 respectively. In terms of host range, none of the weed species was found to be a carrier of Fusarium pathogen implying that it is host specific or semi selective. The results revealed that the tested ecological parameters favor the growth and development of Fusarium pathogen. Thus, management of the disease requires nutrients replenishment and soil shading as essential components in developing appropriate strategies for the control and prevention of further spread of the disease.

Environmental Factors Affecting Parasitism to Cucumber Powdery Mildew Fungus, Sphaerotheca fusca by Ampelomyces quisqualis 94013 and Its Host Range (Ampelomyces quisqualis 94013의 오이 흰가루병균 기생에 영향을 미치는 환경조건과 기주범위)

  • Lee, Sang-Yeob;Ryu, Jae-Dang;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.11 no.2
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    • pp.167-172
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    • 2005
  • An isolate of Ampelomyces quisqualis 94013 was selected as an effective parasite for biological control against cucumber powdery mildew. Temperature range for the parasitism of A. quisqualis 94013 against cucumber powdery mildew was $12\~30^{\circ}C$, and optimal temperature for that $20\~28^{\circ}C$. In $20\~35\%$ humidity of the greenhouse, parasitic ability of A. quisqualis 94013 against Sphaerotheca fusca was not good. Inoculation tests revealed that A. quisqualis 94013 can parasitize on six species of Sphaerotheca in the 12 crops and Ersiphe cichoracerum in tomato. As host rang of A. quisqualis 94013 was broad and it may be used effectively as a biocontrol agent for powdery mildew of 13 crops.

Isolation and Characterization of Bacteriophages Against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit

  • Yu, Ji-Gang;Lim, Jeong-A;Song, Yu-Rim;Heu, Sunggi;Kim, Gyoung Hee;Koh, Young Jin;Oh, Chang-Sik
    • Journal of Microbiology and Biotechnology
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    • v.26 no.2
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    • pp.385-393
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    • 2016
  • Pseudomonas syringae pv. actinidiae causes bacterial canker disease in kiwifruit. Owing to the prohibition of agricultural antibiotic use in major kiwifruit-cultivating countries, alternative methods need to be developed to manage this disease. Bacteriophages are viruses that specifically infect target bacteria and have recently been reconsidered as potential biological control agents for bacterial pathogens owing to their specificity in terms of host range. In this study, we isolated bacteriophages against P. syringae pv. actinidiae from soils collected from kiwifruit orchards in Korea and selected seven bacteriophages for further characterization based on restriction enzyme digestion patterns of genomic DNA. Among the studied bacteriophages, two belong to the Myoviridae family and three belong to the Podoviridae family, based on morphology observed by transmission electron microscopy. The host range of the selected bacteriophages was confirmed using 18 strains of P. syringae pv. actinidiae, including the Psa2 and Psa3 groups, and some were also effective against other P. syringae pathovars. Lytic activity of the selected bacteriophages was sustained in vitro until 80 h, and their activity remained stable up to 50℃, at pH 11, and under UV-B light. These results indicate that the isolated bacteriophages are specific to P. syringae species and are resistant to various environmental factors, implying their potential use in control of bacterial canker disease in kiwifruits.

Biological and Molecular Characterization of a Korean Isolate of Orthotospovirus chrysanthinecrocaulis (Formerly Chrysanthemum Stem Necrosis Virus) Isolated from Chrysanthemum morifolium

  • Seong Hyeon Yoon;Su Bin Lee;Eseul Baek;Ho-Jong Ju;Ju-Yeon Yoon
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.286-294
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    • 2023
  • Biological and molecular characterization of a Korean isolate of Orthotospovirus chrysanthinecrocaulis (formerly known as chrysanthemum stem necrosis virus, CSNV) isolated from Chrysanthemum morifolium was determined using host range and sequence analysis in this study. Twenty-three species of indicator plants inoculated mechanically CSNV-Kr was investigated for determination of host range. CSNV-Kr induced various local and systemic symptoms in the inoculated plant species. CSNV-Kr could not infect three plant species and induced symptomless in systemic leaves in Nicotiana tabacum cultivars, though the plant samples reacted positively with the antiserum to CSNV by double-antibody sandwich-enzyme-linked immunosorbent assay. The complete genome sequence of CSNV-Kr was determined. The L RNA of CSNV-Kr consists of 8,959 nucleotides (nt) and encodes a putative RNA-dependent RNA polymerase. The M RNA of CSNV-Kr consists of 4,835 nt and encodes the movement protein (NSm) and the glycoprotein precursor (Gn/Gc protein). The S RNA of CNSV-Kr consists of 2,836 nt and encodes NSs protein and N protein. The Gn/Gc and N sequence of CSNV-Kr were compared with those of previously published CSNV isolates originating from different countries at nucleotide and amino acid levels. The Gn/GC sequence of CSNV-Kr shared 98.8-99.5% identity with CSNV isolated from other countries and the N sequence of CSNV-Kr shared 98.8-99.6% identity. No particular region of variability could be found in either grouping of viruses. All of the CSNV isolates did not show any relationship according to geographical origins and isolation hosts, suggesting no distinct segregation of the CSNV isolates.

A Performance Comparison of Ad-hoc Wireless Network Routing Protocols (Ad Hoc 무선망의 Routing Protocol 성능 비교분석)

  • 김동일
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.7 no.2
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    • pp.208-213
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    • 2003
  • Recently, a variety of new routing protocols targeted specifically wireless mobile nodes environment have been developed. This paper presents the results of a detailed packet level simulation comparing two multi-hop wireless ad-hoc network routing protocols that cover a range of design choices between DSDV and DSR. And we suggest a performance comparison of network routing protocols through NS-2 simulator.

The Dependence of Type Ia Supernova Luminosities on the Global and Local Properties of Host Galaxies in the YONSEI Supernova Catalog

  • Kim, Young-Lo;Kang, Yijung;Lee, Young-Wook
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.62.3-63
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    • 2018
  • Trends of Type Ia supernova (SN Ia) luminosities with the properties of host galaxies are important to study the underlying physics for an SN progenitor system and explosion mechanism. In the YONSEI SN catalog, we have a sample of ~600 SN and host data in the wider redshift range, and two independent light-curve models, SALT2 and MLCS2k2. From this catalog, here we present that SNe Ia in low-mass, globally and locally star-forming environments are fainter than those in high-mass, globally and locally passive environments, after light-curve shape and color or extinction corrections. Our results are then compared to previous studies, and show consistent results.

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Whole Genome Enabled Phylogenetic and Secretome Analyses of Two Venturia nashicola Isolates

  • Prokchorchik, Maxim;Won, Kyungho;Lee, Yoonyoung;Segonzac, Cecile;Sohn, Kee Hoon
    • The Plant Pathology Journal
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    • v.36 no.1
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    • pp.98-105
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    • 2020
  • Venturia nashicola is a fungal pathogen causing scab disease in Asian pears. It is particularly important in the Northeast Asia region where Asian pears are intensively grown. Venturia nashicola causes disease in Asian pear but not in European pear. Due to the highly restricted host range of Venturia nashicola, it is hypothesized that the small secreted proteins deployed by the pathogen are responsible for the host determination. Here we report the whole genome based phylogenetic analysis and predicted secretomes for V. nashicola isolates. We believe that our data will provide a valuable information for further validation and functional characterization of host determinants in V. nashicola.