• Title/Summary/Keyword: Host range

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Characterization of Peanut stunt virus Isolated from Black Locust Tree (Robinia pseudo-acacia L.)

  • Bang, Ju-Hee;Choi, Jang-Kyung;Lee, Sang-Yong
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.125-130
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    • 2006
  • An isolate of Peanut stunt virus (PSV) isolated from black locust tree (Robinia pseudo-acacia L.) showing severe mosaic and malformation symptoms, was designated as PSV-Rp. PSV-Rp was characterized by the tests of host range, physical properties, RNA and coat protein composition and RT-PCR analysis. Nucleotide sequences of the cucumoviruses CP genes were also used for identification and differentiation of PSV-Rp. Six plant species were used in the host range test of PSV-Rp. PSV-Rp could be differentiated from each Cucumovirus strain used as a control by symptoms of the plants. The physical properties of PSV-Rp virus were TIP $65^{\circ}C$, DEP $10^{-3}$, and LIP $2{\sim}3$ days. In dsRNA analysis, PSV-Rp consisted of four dsRNAs, but satellite RNA was not detected. Analysis of the coat proteins by SDS-PAGE showed one major protein band of about 31 kDa. RT-PCR using a part of Cucumovirus RNA3 specific primer amplified ${\sim}950bp$ DNA fragments from the crude sap of virus-infected black locust leaves. RFLP analysis of the RT-PCR product could differential PSV-RP from CMV The nucleotide sequence identity between the PSV-Rp CP and the TAV-P CP genes and the PS-V-RP CP and CMV-Y CP genes were 61.6% and 40.5%, respectively. On the other hand, the nucleotide sequence identity of the PSV-Rp CP gene was $70.9%{\sim}73.4%$ in comparison with those of PSV subgroup I (PSV-ER and PSV-J) and 67.3% with that of PSV subgroup II(PSV-W). Especially, the nucleotide sequence identity of PSV-Rp CP gene and that of PSV-Mi that was proposed recently as the type member of a novel PSV subgroup III was 92.4%.

Characterization of Recombinant Baculovirus Ewpressing Polyhedrin Gene of Bombyx mori and Autographa californica Nuclear Polyhedrois Virus (두 종류의 다각체 단백질 유전자를 발현하는 유전자 재조합 핵다각체병 바이러스의 특성)

  • 김우진;우수동
    • Journal of Sericultural and Entomological Science
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    • v.38 no.1
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    • pp.36-41
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    • 1996
  • Recombinant baculoviruses having expanded host range were selected by coinfection of Autographa california NPV and Bombyx mori NPV into Sf-9 and BmN-4 insect cell lines. In order to determine the polyhedra morhplogy of RecS-A6, one of a recombinant baculovirus, polyhedra of RecS-A6 produced in insect cells were observed by phase contrast microscope and scanning electron microscope. The results revealed that the recombinant baculovirus had a various polyhedra morphology which was different from its parental viruses, suggesting that the various morhpology of recombinant baculovirus with an expanded host range was due to the genetic recombination of viral genome. To analyze the genomic recombinantion of the recombinant baculoviruses, genomic DNAs of two parent viruses and RecS-A6 were digested with restriction endonuclease and subjected to agarose gel electrophoresis. Southern blot analysis revealed that RecS-A6 has two polyhedrin gene of AcNPV and BmNPV in a viral genome. Polyhedral protein of recombinant baculovirus was analysed by SDS-PAGE. The result showed that molecular weight of polyhedral protein of RecS-A6 containing two polyhedrin gene of AcNPV and BmNPV was as the 31 kDa band of AcNPV and 30 kDa band of BmNPV.

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First Report of Zucchini yellow mosaic virus on Hollyhock (Althaea rosea)

  • Park, Won-Mok;Park, Seung-Kook;Yoon, Ju-Yeon;Ryu, Ki-Hyun;Park, Jang-Kyung
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.121-125
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    • 2002
  • This study was conducted to determine the causal virus that naturally infected hollyhock (Althaea rosea) plant showing mild mosaic symptom in 1999. Flexuous virus particles were found in the cytoplasm of plant tissue from infected hollyhock under transmissible electron microscopy. A virus from the genus Potyvirus under the family Potyviridae was isolated and was maintained on Chenopodium quinoa for three passages. Chlorotic local legions were used to inoculate 20 species of indicator plants. The virus infected all the tested cucurbit plants, but failed to infect Nicotiana benthamiana. Based on the host range test and RT-PCR analysis, the potyvirus was identified as a strain of Zucchini yellow mosaic virus-A (ZYMV-A), one of the major pathogens of cucurbits. Infectivity analysis showed that ZYMV-A induced faster systemic symptom than ZYMV-Cu on squash and other cucurbit plants, suggesting that ZYMV-A was a more severe strain. To better characterize ZYMV-A, Western blot assay was carried rout to the coat protein (CP) of the virus using ZYMV-specific antiserum with ZYMV-Cu and other potyviruses. The CP of the virus reacted strongly with the antiserum against ZYMV, and other tested antisera did not react with the CP of ZYMV-A. Results strongly suggest that the potyvirus infecting hollyhock was a novel strain of ZYMV. This is the first report on ZYMV as the causal virus infecting hollyhock in Korea.

Pathogenicity and Host Range of a Potential Mycoherbicide, Isolate BWC98-105, Causing White Root Rot on Trifoliorum repens

  • Hong, Yeon-Kyu;Cho, Jae-Min;Lee, Bong-Choon;Song, Seok-Bo;Park, Sung-Tae
    • The Plant Pathology Journal
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    • v.20 no.1
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    • pp.58-62
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    • 2004
  • White root rot of wild white clover (Trifoliorum repens) caused by isolate BWC98-105 has been first reported in Korea. Typical symptoms on root include water-soaked and dark-brown rot, resulting in complete blight of the whole plant. The fungus grew well at $20-28^{\circ}C$ and produced abundant sclerotia at 10-15 days after full mycelial growth on potato dextrose agar. Sclerotia were brown to dark-brown in color and 1-3 mm in length. When white clover plants were inoculated with mycelial suspension ($10^5$ cfu/ml) of isolate BWC98-105, the plant shoots were killed within 4-6 days and the roots were completely blighted. Sclerotia were also formed on the surface of the root covered with whitish mycelia within 10-15 days in the field. All nine isolates developed high incidences of white root rot disease on white clover seedlings, of which the symptoms were similar to those observed in the fields. Hence, their pathogenicity was confirmed on white clover. The infection rate of the fungal isolates varied from 78.5% to 95.2%, among which BWC98-105 was the most virulent isolate. The weeding efficacy of the fungus was maintained until the following year, leading to a significant reduction of reshooting. The fungus was specifically parasitic to white clover, but not to four lawn species including zoysiagrass (Zoysia japonica) under greenhouse test. The fungus also had no response to some Gramineae species including rice, but caused little damage to five species of Leguminosae.

DETECTION OF WIDE PLANETARY SYSTEM WITH MICROLENSING

  • Ryu, Yoon-Hyun;Park, Myeong-Gu;Chang, Heon-Young;Lee, Ki-Won
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.108.2-108.2
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    • 2012
  • Recent results from microlensing surveys show that a free-floating planet or a wide-separation planet is more numerous than a main-sequence star in the Galaxy. Moreover, the detection efficiency of the planets will be improved in next-generation experiments with a high survey monitoring frequency. However, microlensing events produced by both planets appear similar light curves with a short duration timescale, thus it is difficult to distinguish them. In this paper, we investigated the detectable separation range of a wide-separation planet as the planet bound to its host star. We construct the fractional deviation maps using the magnifications of the planetary lensing and the single-lensing by planet itself for various parameters such as a mass ratio, separation, and source radius. As a result, we found that the pattern of the fractional deviation is related to the ratio of source radius to caustic size, and the ratio satisfying the detection criterion (i.e., ${\geq}5%$ in the fractional deviation) varies with a separation. Hence, we derived a fitting formula as the function of a mass ratio and a source radius to reflect the variation in the calculations of the detectable separation range of a wide-separation planet as the planet bound to its host star. In addition, we estimated the condition that a wide-separation planet can be detected as a single-lensing event under the finite source effect. We found that such a case is possible provided that the source radius is smaller than ~2.5 times of Einstein ring radius of a planet, regardless of a separation or a mass ratio.

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The Aphid-attacking Fungus Pandora neoaphidis; the First Observation and its Host Range in Korea (국내 미기록 진딧물병원성 곰팡이, Pandora neoaphidis에 관한 보고)

  • Yoon, Cheol-Sik;Sung, Gi-Ho;Choi, Byung-Ryul;Yoo, Jai-Ki;Lee, Jeang-Oon
    • The Korean Journal of Mycology
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    • v.26 no.3 s.86
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    • pp.407-410
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    • 1998
  • The aphid-attacking fungus Pandora neoaphidis was collected during May and June, 1998 at various localities: two localities at Kyunggi, three at Kangwon, one at Kyungbuk and one at Chungnam Provinces. Occurrence of this fungus has never been recorded in Korea. The potential host aphids are Acyrthosiphon kondoi, Myzus persicae, Lipaphis erysimi, Sitobion avenae, Aphis rumicis, Dactynotus species and unknown species. Microscopic characters of the fungus are described and illustrated. Pandora neoaphidis studied in this study has an extremely broad range in conidial size and shape even within a single specimen. Such variable size and shape are typical characters of the fungus. During microscopic examination of specimens, the resting-spore phase was not observed.

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Probiotic Characteristics of Lactobacillus brevis KT38-3 Isolated from an Artisanal Tulum Cheese

  • Hacioglu, Seda;Kunduhoglu, Buket
    • Food Science of Animal Resources
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    • v.41 no.6
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    • pp.967-982
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    • 2021
  • Probiotics are living microorganisms that, when administered in adequate amounts, provide a health benefit to the host and are considered safe. Most probiotic strains that are beneficial to human health are included in the "Lactic acid bacteria" (LAB) group. The positive effects of probiotic bacteria on the host's health are species-specific and even strain-specific. Therefore, evaluating the probiotic potential of both wild and novel strains is essential. In this study, the probiotic characteristics of Lactobacillus brevis KT38-3 were determined. The strain identification was achieved by 16S rRNA sequencing. API-ZYM test kits were used to determine the enzymatic capacity of the strain. L. brevis KT38-3 was able to survive in conditions with a broad pH range (pH 2-7), range of bile salts (0.3%-1%) and conditions that simulated gastric juice and intestinal juice. The percentage of autoaggregation (59.4%), coaggregation with E. coli O157:H7 (37.4%) and hydrophobicity were determined to be 51.1%, 47.4%, and 52.7%, respectively. L. brevis KT38-3 produced β-galactosidase enzymes and was able ferment lactose. In addition, this strain was capable of producing antimicrobial peptides against the bacteria tested, including methicillin and/or vancomycin-resistant bacteria. The cell-free supernatants of the strain had high antioxidant activities (DPPH: 54.9% and ABTS: 48.7%). Therefore, considering these many essential in vitro probiotic properties, L. brevis KT38-3 has the potential to be used as a probiotic supplement. Supporting these findings with in vivo experiments to evaluate the potential health benefits will be the subject of our future work.

Isolation of bacteriophage-resistant Pseudomonas tolaasii strains and their pathogenic characters (박테리오파지 저항성을 갖는 Pseudomonas tolaasii 변이주 분리 및 이들의 병원특성)

  • Park, Soo-Jin;Han, Ji-Hye;Kim, Young-Kee
    • Journal of Applied Biological Chemistry
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    • v.59 no.4
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    • pp.351-356
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    • 2016
  • Bacterial blotch caused by Pseudomonas tolaasii is one of the major diseases of oyster mushroom, Pleurotus ostreatus. Application of bacteriophages is a very useful tool to decrease the density of pathogens and it has been successful to making disease-free cultivation area, known as phage therapy. Effect of phages on pathogen sterilization is very limited to the specific host strains. Minor variations of the host strains may cause changes in phage sensitivity. The phage-resistant strains of P. tolaasii were isolated and their pathogenic characters were investigated to improve the effectiveness of phage therapy. In the phylogenetic analysis, both phage-resistant strains and the corresponding host strains were identical based on the sequence comparison of 16S rRNA genes. The pathogenic characters, such as hemolytic activity and brown blotch formation, were measured on the phage-resistant strains and no correlation between phage-resistance and pathogenic characters was observed. Nevertheless, pathogenic characters were sometimes changed in the phage-resistant strains depending on the host strains. In order to make the phage therapy successful, the bacteriophages having a wide host range should be isolated.

Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia

  • Raza, Ahmed;Al-Shahwan, Ibrahim M.;Abdalla, Omer A.;Al-Saleh, Mohammed A.;Amer, Mahmoud A.
    • The Plant Pathology Journal
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    • v.33 no.1
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    • pp.43-52
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    • 2017
  • A survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA indicated that 59 of these samples were positive to LTSV. Two isolates of LTSV from each region were selected for molecular studies. RT-PCR confirmed the presence of LTSV in the selected samples using a specific primer pair. Percentage identity and homology tree comparisons revealed that all Saudi isolates were more closely related to each other but also closely related to the Canadian isolate-JQ782213 (97.1-97.6%) and the New Zealand isolate-U31286 (95.8-97.1%). Comparing Saudi isolates of LTSV with ten other sobemoviruses based on the coat protein gene sequences confirmed the distant relationship between them. Eleven out of fourteen plant species used in host range study were positive to LTSV. This is the first time to document that Trifolium alexandrinum, Nicotiana occidentalis, Chenopodium glaucum, and Lathyrus sativus are new host plant species for LTSV and that N. occidentalis being a good propagative host for it.

Host range, Life cycle and Natural enemies of Mulberry scale (Pseudaulacaspis pentagona) on Prunus mume in south Korea (매실나무에 기생하는 뽕나무깍지벌레 (Pseudaulacaspis pentagona)의 기주범위, 생활사 및 천적에 관한 조사)

  • 박종대;김규진
    • Korean journal of applied entomology
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    • v.29 no.2
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    • pp.104-112
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    • 1990
  • Studies were conducted to investigate hosts, life cycle, population dynamics and natural enemies of mulberry scale, Pseudaulacaspis pentagona Ta. & Tozz., from 1987 to 1988 in southern region of Korea. The host plants were 22 families and 74 species. Host plants belonged to genus Prunus in general were severely damaged due to mulberry scale. Mulberry scale was most likely to have three generations a year with first occurrence of larval stage from mid-May to late June, second occurrence from mid-July to mid-August and third occurrence from early September to late October including 3 peaks in early and mid-June, late July and mid-September. In case of each stage occurrence, eggs were peaked on 10th day, larvae on 25th day and adults on 7th day from beginning of occurrence, respectively. Natural enemies were observed as parasitoids of 3 species such as Aphytis diaspidis, Archenomus orientalis and Apterencyrtus mocrophagus and as predators of 3 species such as Chilocorus Kuwanae, Chilocorus rubidus and Hormonia axyridis. Shoot growth was affected by mulberry scale and Lepra to dwindle as much as half of normal growth.

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