• Journal of the Korean Society of Environmental Restoration Technology
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• v.20 no.3
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• pp.55-64
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• 2017

The problem of the population number of honeybees that is decreasing not only domestically but also globally, has a great influence on human beings and the entire ecosystem. The habitat of honeybees is recognized to be superior in urban environment rather than rural environment, and predicting for habitat assessment and conservation is necessary. Based on this, we targeted Cheonan City and neighboring administrative areas where the distribution of agricultural areas, urban areas, and forest areas is displayed equally. In order to predict the habitat preferred by honeybees, we apply the Maxent model what based on the presence information of the species. We also selected 10 environmental variables expected to influence honeybees habitat environment through literature survey. As a result of constructing the species distribution model using the Maxent model, 71.7% of the training data were shown on the AUC(Area Under Cover) basis, and it was be confirmed with an area of 20.73% in the whole target area, based on the 50% probability of presence of honeybees. It was confirmed that the contribution of the variable has influence on land covering, distance from the forest, altitude, aspect. Based on this, the possibility of honeybee's habitat characteristics were confirmed to be higher in wetland environment, in agricultural land, close to forest and lower elevation, southeast and west. The prediction of these habitat environments has significance as a lead research that presents the habitat of honeybees with high conservation value of ecosystems in terms of urban space, and it will be useful for future urban park planning and conservation area selection.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.2
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• pp.99-105
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• 2005

We investigated oviposition and colony development of Bombus ignitus and B. terrestris depending on different pollen. In five kinds of pollen blend, the colony development in fresh-freezing pollen blend produced in Korea (Korea-FFP) was the best performance, although egg-laying characteristics is lower than that of pollen blend for oviposition imported from Korppert company (Kopport-FOP). The Kopport-FOP proved that it was suitable to use for oviposition of bumblebees. The Korea-FFDP, freezing dried-fresh pollen blend produced in Korea, is lower rather than the Korea-FDP although it is similar to the KopportFOP in colony development. It is not efficient to use commercial pollen for bumblebee because it is expensive in cost. The dried pollen blend for honeybee feeding imported from China (China-DP) was not suitable for rearing of bumblebee because it did not form colony although the worker emerged. In types of pollen, the oviposition and colony development of B. ignitus were not affected by the fresh-freezing pollen and dried-freezing pollen. This result also indicated that dried pollen, dried in the shade for 5 - 6 days, is possible to use as commercial pollen for bumblebee reproduction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.5
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• pp.689-694
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• 2003

Propolis obtained from honeybee hives has been used in oriental folk medicine as an anti-inflammatory, anti-carcinogenic, or immunomodulatory agent. To prepare granule depending on operational parameters, such as glucose content to total sugar (X$_1$, 0~100%), ethanol concentration (X$_2$, 20~100%) and sprayed ethanol solution content (X$_3$, 6~10%) using propolis, response surface methodology was applied to monitor the changes in yield, fragmentation rate by shaking and organoleptic properties. Yield showed high with decreasing sprayed ethanol solution content and fragmentation rate by shaking decreased with increasing sprayed ethanol solution content. The organoleptic color, flavor, taste, mouth-feel and overall palatability were dependent on the glucose content to total sugar, ethanol concentration and sprayed ethanol solution content. Overall palatability was maximized in glucose content to total sugar 47.94%, ethanol concentration 56.45% and sprayed ethanol solution content 8.04% .

• BMB Reports
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• v.38 no.1
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• pp.49-57
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• 2005

Major Royal Jelly Protein cDNAs of Apis cerana (AcMRJP) were cloned and characterized. The open reading frames (ORFs) of the AcMRJP1 and AcMRJP2 genes were 1302 and 1392 nucleotides, encoding 433 and 463 amino acid residues, respectively. The sequence divergences between AcMRJP1 and AcMRJP2 and their corresponding protein families in A. mellifera were 0.0618 and 0.0934 at the nucleotide level and 0.0912 and 0.1438 at the protein level, respectively. Phylogenetic analysis supports the orthologous similarity between these proteins. The deduced amino acids indicated high essential amino acid contents of AcMRJP1 and AcMRJP2 (47.5 and 44.8%, respectively). The genomic organization of both AcMRJP1 and AcMRJP2 was determined. Both the AcMRJP1 (3663 bp) and AcMRJP2 (3963 bp) genes contained six exons and five introns, where all boundaries conformed to the GT/AG rule. AcMRJP1 and AcMRJP2 cDNAs were cloned into pET17b, and both the recombinant (r) AcMRJP1 (47.9 kDa) and rAcMRJP2 (51.7 kDa) were expressed in the insoluble form. Western blot analysis and N-terminal sequencing of the solubilized proteins revealed successful expression of rAcMRJP1 and rAcMRJP2 in vitro. The yields of the purified rAcMRJP1 and rAcMRJP2 were approximately 20 and 8mg protein per liter of the flask culture, respectively.

• International Journal of Industrial Entomology and Biomaterials
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• v.31 no.2
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• pp.62-69
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• 2015

The Asian cavity-nesting honeybee, Apis cerana (Hymenoptera: Apidae), has been extensively studied for its biogeography and genetic diversity, but the molecules utilized in past studies were mainly ~90 bp long mitochondrial non-coding sequences, located between $tRNA^{Leu}$ and COII. Thus, additional molecular markers may enrich our understanding of the biogeography and genetic diversity of this valuable bee species. In this study, we reviewed the public genome database to find introns of cDNA sequences, with the assumption that these introns may have less evolutionary constraints. The six introns selected were subjected to preliminary tests. Thereafter, two introns, titled White gene and MRJP9 gene, were selected. Sequencing of 552 clones from 184 individual bees showed a total of 222 and 141 sequence types in the White gene and MRJP9 gene introns, respectively. The sequence divergence ranged from 0.6% to 7.9% and from 0.26% to 17.6% in the White gene and the MRJP9 introns, respectively, indicating higher sequence divergence in both introns. Analysis of population genetic diversity for 16 populations originating from Korea, China, Vietnam, and Thailand shows that nucleotide diversity (π) ranges from 0.003117 to 0.025837 and from 0.016541 to 0.052468 in the White gene and MRJP9 introns, respectively. The highest π was found in a Vietnamese population for both intron sequences, whereas the nine Korean populations showed moderate to low sequence divergence. Considering the variability and diversity, these intron sequences can be useful as non-mitochondrial DNA-based molecular markers for future studies of population genetics.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.3
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• pp.279-286
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• 2016

Caffeic acid phenethyl ester (CAPE), derived from honeybee hives, is a bioactive compound with strong antioxidant activity. This study was designed to test the neuroprotective effect of CAPE in 3-nitropropionic acid (3NP)-induced striatal neurotoxicity, a chemical model of Huntington's disease (HD). Initially, to test CAPE's antioxidant activity, a 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) antioxidant assay was employed, and CAPE showed a strong direct radical-scavenging effect. In addition, CAPE provided protection from 3NP-induced neuronal cell death in cultured striatal neurons. Based on these observations, the in vivo therapeutic potential of CAPE in 3NP-induced HD was tested. For this purpose, male C57BL/6 mice were repeatedly given 3NP to induce HD-like pathogenesis, and 30 mg/kg of CAPE or vehicle (5% dimethyl sulfoxide and 95% peanut oil) was administered daily. CAPE did not cause changes in body weight, but it reduced mortality by 29%. In addition, compared to the vehicle-treated group, robustly reduced striatal damage was observed in the CAPE-treated animals, and the 3NP-induced behavioral deficits on the rotarod test were significantly rescued after the CAPE treatment. Furthermore, immunohistochemical data showed that immunoreactivity to glial fibrillary acidic protein (GFAP) and CD45, markers for astrocyte and microglia activation, respectively, were strikingly reduced. Combined, these data unequivocally indicate that CAPE has a strong antioxidant effect and can be used as a potential therapeutic agent against HD.

• Journal of Ecology and Environment
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• v.38 no.3
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• pp.367-374
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• 2015

The aim of this study was to assess the occurrence and foraging of social and other wasps and bees in Jirisan National Park (JNP, South Korea), in particular in an apiary. Sixty one traps were placed in the southwestern part of JNP to identify social wasps from July to September 2014, and the damage to the apiary caused by wasps or bees was observed once a month from May to December 2014 between 10 a.m. and 5 p.m. In total, 10 species of Vespidae were collected by trapping. Vespa crabro was the most abundant (245 individuals, 28.3%), followed by Vespa velutina (162 individuals, 18.7%). In the apiary, however, V. velutina was the most frequent species. V. velutina visited a maximum of 167 times a day in September, which corresponded to one visit in 2.5 min. Accordingly, these data are in line with the most serious impact of V. velutina on the apiaries in South Korea. V. simillima was the second most frequent species; both Vespa species hawked honeybees. Even though the occurrence of V. mandarinia was low, this species caused serious damage by mass slaughter of honeybees. The occurrence of V. crabro, V. analis and V. ducalis was quite low and their impact on honeybees was negligible. There have been few reports of V. dybowskii foraging for honeybees, but they are considered to be a new pest because their impact on apiaries is considerable. Most Vespa species attacked the apiary from June to October, with a maximum in September. However, V. velutina visited until November to early December. Vespula species are not more serious pests than Vespa species, but many adults were observed stealing honey from beehives. Polistes, Orancistrocerus, and Bombus species had no impact on honeybee colonies in the apiary.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.9-13
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• 2003

Cuticular hydrocarbons of antenna, legs and wings from two species of honeybee worker of Apis mellifera L. and Apis cerana F. can be analyzed directly with gas chromatograph and GC/MS without solvent extraction. The saturated hydrocarbons identified in selected part of both species were nC22, nC23, nC25-nC3O, nC32 and nC34 except nC24. Two saturated hydrocarbons, nC26 (23.0-42.6%) and nC28 (16.8-54.8%), were major compounds in both species and others were minor compounds. A. mellifera can be distinguished from A. cerana F. by having higher proportion of nC30, nC32 and nC34 by having lower proportion of nC25 from three selected part of both species.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.99-103
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• 2000

The green lacewing, Chrysopa pallens (Rambur), was reared on artificial diets supplementedwith natural products such as lyophilized beef liver, silk worm pupae powder and drone honey beepowder. The performance of the green lacewing was improved siginificantly on only the diet to whichadded 1 % of silk worm pupae powder (wt/v). Larval developement was completed in 15.6 f 0.99 days,cocoon weight was 16.4f2.94 mg, and suvival to adult was 89.3% at 27$^{\circ}$C. And another diet made bymixing 4 part of lyophilized beef liver powder, 4 part of lyophilized beef powder, 3 part of lyophilizedchicken egg, 4 part of sucrose, and 50 mg each of vitamin C and Vanderzant's vitamin B mixture waspromising. Larval development of the green lacewing on this diet was completed in 12.7 f 1.45 days at27"C, pupal weight was 18.1 f 2.24mg, suvival to adult was loo%, and 633f70.4 eggs were laid in 20days after beginning of oviposition.ginning of oviposition.

• Korean Journal of Veterinary Service
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• v.38 no.1
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• pp.25-30
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• 2015

Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compounds in some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks of dairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animal drugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for its residue has not been established yet in Korea. Therefore, the purpose of this study was to develop pre-processing method not affecting major component of bee venom for detection of its residue in milks using ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was also analyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with the developed pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, the major components of bee venom, were all detected by UHPLC with the pre-processing method developed in this study. The results of this study suggest that the pre-processing method developed in this study can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venom residues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and 3 days, respectively.