• Bulletin of the Korean Chemical Society
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• v.24 no.9
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• pp.1339-1344
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• 2003

Flow field-flow fractionation (FlFFF) is a technology to separate the molecules by size in an open channel. Molecules with different size have different diffusivities and are located vertically in different positions when passing through an open channel. In this study, hollow fiber membranes instead of conventional rectangular channels have been used as materials for the open channel and this change would decrease the cost of manufacturing. FlFFF is a useful technique to characterize the biopolymeric materials. Retention time, diffusion coefficients and Stokes radius of analysis can be calculated from the related simple equations. Hollow-fiber flow field-flow fractionation (HF-FlFFF) has been used for the characterization and separation of protein mixture in a phosphate buffer solution and has demonstrated the potential to be developed into a disposable FlFFF channel. The important indexes for the analytical separation are selectivity, resolution and plate height. The optimized separation condition for protein mixture of Ovalbumin, Alcohol dehydrogenase, Apoferritin and Thyroglobulin is ${\dot V}_{out}/{\dot V}_{rad}=0.65/0.85\;mL/min$.

• Proceedings of the Membrane Society of Korea Conference
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• 2004.05b
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• pp.192-194
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• 2004

FFF에서 외부장은 시료의 종류에 따라 선택적으로 사용되며, 그 외부장의 종류에 따라 세부기술로 구분되어진다. 원심력(gravitational or centrifugal field)을 외부장으로 사용하는 침강장-흐름분획법(sedimentation FFF)은 지름이 0,05∼1um정도인 콜로이드 입자의 분리에 좋은 방법이다. 온도구배(thermal fradient)를 이용하는 열장-흐름분획법(thermal FFF)은 유기 고분자의 분리 및 고분자의 물성연구에 이용된다.(중략)

• Bulletin of the Korean Chemical Society
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• v.24 no.9
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• pp.1333-1338
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• 2003

Hollow Fiber flow field-flow fractionation (HF-FlFFF) has been tested in polyacrylonitrile (PAN) membrane channel in order to compare it with polysulfone (PSf) membrane channel. It has been experimentally shown that the separation time of 0.05-0.304 ${mu}m$ polystyrene latex (PSL) standards in PAN membrane channel is shorter than that in PSf channel by approximately 65%. The optimized separation condition in PAN membrane is ${\dot V}_{out}/{\dot V}_{rad}=1.4/0.12\;mL/min$, which is equal to the condition in PSf membrane channel. In addition both the resolution ($R_s$) and plate height (H) in PAN membrane channel are better than that in PSf membrane channel. The membrane radius was obtained by back calculation with retention time. It shows that the PSf membrane is expanded by swelling and pressure, but the PAN membrane doesn't expand by swelling and pressure.