• Title/Summary/Keyword: Hog1

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Role of MAPK Signaling Pathways in Regulating the Hydrophobin Cryparin in the Chestnut Blight Fungus Cryphonectria parasitica

  • So, Kum-Kang;Kim, Dae-Hyuk
    • Mycobiology
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    • v.45 no.4
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    • pp.362-369
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    • 2017
  • We assessed the regulation of cryparin, a class II hydrophobin, using three representative mitogen-activated protein kinase (MAPK) pathways in Cryphonectria parasitica. Mutation of the CpSlt2 gene, an ortholog of yeast SLT2 in the cell wall integrity (CWI) pathway, resulted in a dramatic decrease in cryparin production. Similarly, a mutant of the CpBck1 gene, a MAP kinase kinase kinase gene in the CWI pathway, showed decreased cryparin production. Additionally, mutation of the cpmk1 gene, an ortholog of yeast HOG1, showed decreased cryparin production. However, mutation of the cpmk2 gene, an ortholog of yeast Kss1/Fus3, showed increased cryparin production. The easy-wet phenotype and accumulation of the cryparin transcript in corresponding mutants were consistent with the cryparin production results. In silico analysis of the promoter region of the cryparin gene revealed the presence of binding motifs related to downstream transcription factors of CWI, HOG1, and pheromone responsive pathways including MADS-box- and Ste12-binding domains. Real-time reverse transcriptase PCR analyses indicated that both CpRlm1, an ortholog of yeast RLM1 in the CWI pathway, and cpst12, an ortholog of yeast STE12 in the mating pathway, showed significantly reduced transcription levels in the mutant strains showing lower cryparin production in C. prasitica. However, the transcription of CpMcm1, an ortholog of yeast MCM1, did not correlate with that of the mutant strains showing downregulation of cryparin. These results indicate that three representative MAPK pathways played a role in regulating cryparin production. However, regulation varied depending on the MAPK pathways: the CWI and HOG1 pathways were stimulatory, whereas the pheromone-responsive MAPK was repressive.

Triangle Method for Fast Face Detection on the Wild

  • Malikovich, Karimov Madjit;Akhmatovich, Tashev Komil;ugli, Islomov Shahboz Zokir;Nizomovich, Mavlonov Obid
    • Journal of Multimedia Information System
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    • v.5 no.1
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    • pp.15-20
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    • 2018
  • There are a lot of problems in the face detection area. One of them is detecting faces by facial features and reducing number of the false negatives and positions. This paper is directed to solve this problem by the proposed triangle method. Also, this paper explans cascades, Haar-like features, AdaBoost, HOG. We propose a scheme using 12-net, 24-net, 48-net to scan images and improve efficiency. Using triangle method for frontal pose, B and B1 methods for other poses in neural networks are proposed.

Effects of dietary protein of hog hair on the nutrients metabolism in poultry (돈모 단백질의 급여가 닭의 영양소 대사에 미치는 영향)

  • Oh, Hyun-Min;Park, Noh-Sung;Jo, Chi-Hyun;Kim, Sung-Bok;Lim, Jae-Sam;Lee, Bong-Duk;Lee, Soo-Kee
    • Korean Journal of Agricultural Science
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    • v.38 no.4
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    • pp.651-657
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    • 2011
  • Two experiments were conducted to investigate the effect of feeding hog hair protein (HHP) on the nutritional value for poultry. In experiment 1, twenty roosters of Hanhyup-3 strain were alloted, and metabolizability of HHAA was measured. In experiment 2, forty roosters of Hanhyup-3 strain were alloted to 0, 3, 7.6 and 15.1% of HHAA treatments, 10 birds per treatment, and measured feed utilization and blood parameters. In experiment 1, no trend was found in excretion of amino acid, high in glysine and glutelin, low in valine, threonine, and methionine. HHAA metabolizability of serine, phenylalanine, alanine, and isoleucine was more higher than that of lysine, cystine, asparagine, and tyrosine. In experiment 2, as the HHAA level increased, feed intake decreased significantly in 15.1% treatment, but water intake increased significantly in 15.1% traetment. Dry matter and nitrogen metabolizability decreased in 7.6 and 15.1% treatments. Although no significant difference was found among three treatments(0, 3, and 5.7%), as the HHAA level increased, dry matter and nitrogen metabolizability decreased. Serum creatinine level was significantly increased in 15.1% treatment. In conclusion, it is considered that proper level to substitute soybean meal by HHAA was 10%.

Curvature and Histogram of oriented Gradients based 3D Face Recognition using Linear Discriminant Analysis

  • Lee, Yeunghak
    • Journal of Multimedia Information System
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    • v.2 no.1
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    • pp.171-178
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    • 2015
  • This article describes 3 dimensional (3D) face recognition system using histogram of oriented gradients (HOG) based on face curvature. The surface curvatures in the face contain the most important personal feature information. In this paper, 3D face images are recognized by the face components: cheek, eyes, mouth, and nose. For the proposed approach, the first step uses the face curvatures which present the facial features for 3D face images, after normalization using the singular value decomposition (SVD). Fisherface method is then applied to each component curvature face. The reason for adapting the Fisherface method maintains the surface attribute for the face curvature, even though it can generate reduced image dimension. And histogram of oriented gradients (HOG) descriptor is one of the state-of-art methods which have been shown to significantly outperform the existing feature set for several objects detection and recognition. In the last step, the linear discriminant analysis is explained for each component. The experimental results showed that the proposed approach leads to higher detection accuracy rate than other methods.

Purification and Properties of Protein Methylase I from Hog Pancreas (돼지 췌장내 Protein Methylase I의 분리정제 및 성질)

  • 이향우;장만식
    • YAKHAK HOEJI
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    • v.31 no.3
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    • pp.173-181
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    • 1987
  • Protein methylase I has been partially purified from hog pancreas with a 11% yeild. The final preparation is completely free of any other protein-specific methyltransferases and endogenous substrate proteins. The enzyme has an optimum pH of 7.2 and the approximate molecular weight is above 800 thousands dalton. The Km values for S-adenosyl-L-methionine and histone type II-A are 1.32$\times$10$^{-5}$M. The Ki value for S-adenosyl-L-homocysteine is 1.52$\times$10$^{-6}$M. The effect of enyzme concentration on the activity showed a slight sigmoidal curve suggesting the involvement of certain cofactors. Even though the purified enzyme showed two bands on polyacrylamide gel electrophoresis, the enzyme is highly specific for the arginine residues of protein and specifically, highly specific for histone, suggesting histonespecific protein methylase I.

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The effects of aqueous extracts of plant roots on germination of seeds and growth of seedings (식물근의 추출물질이 종자발아 및 유식물의 생장에 미치는 영향)

  • Chan-Ho Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.1-23
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    • 1968
  • This study aimed at contributing to the improvement of cropping systems after finding out the effects of excrements and components of crop root influence on other crops as well as themselves. The following forage crops suitable for our country were selected for the present study. Aqueous extracts of fresh roots, aqueous extracts of rotting roots and aqueous solutions of excrements of red clover, orchard grass and brome grass were studied for the effects influencing the germination and growth of seedlings of red clover, ladino clover, lespedeza, soybean, orchard grass, Italian ryegrass, brome grass, barley, wheat, sorghum, corn and Hog-millet. In view of the possibility that the organic acid might be closely related to the excrements and components of crop root connected with soil sickness, the acid components of three species of roots were analysed by paper chromatography and gas chromatography method. The following results were obtained: 1. Effects of Aqueous Extracts of Fresh Roots : Aqueous extracts of red clover: The extracts inhibited the growth of seedlings of the ladino clover and lespedeza and also inhibited the development of most crops except that of sorghum among the Graminaceae. Aqueous extracts of orchard grass: The extracts promoted the seedlings growth of red clover and soybean, while it inhibited the germination and growth of orchard grass. There were no noticeable effects influencing other crops while it inhibited the growth of barley and Hog-millet. Aqueous extracts of brome grass: There was no effect on Italian ryegrass but there was an inhibiting effect on the other crops. 2. Effects of Aqueous Extracts of Rotting Roots : Aqueous extracts of red clover: The extracts promoted the seedling growth of red clover. But it reflected the inhibiting effects on other crops except sorghum. Aqueous extracts of orchard grass: The extracts promoted the growth of red clover, ladino clover, soybean and sorghun, while it inhibited the germination and rooting of barley and Hog-millet. Aqueous extracts of brome grass: The extracts gave the promotive effects to the growth of red clover, soybean and sorghum, but caused inhibiting effects on orchard grass, brome grass, barley and Hog-millet. 3. Effects of Aqueous Solutions of Excrements : The aqueous solution of excrements of red clover reflected the inhibition effects to the growth of Graminaceae, while the aqueous solutions of excrements of orchard grass and Italian ryegrass caused the promotive effects on the growth of red clover. 4. Results of Organic Acid Analysis : The oxalic acid, citric acid, tartaric acid, malonic acid, malic acid and succinic acid were included in the roots of red clover as unvolatile organic acid, and in the orchard grass and brome grass there were included the oxalic acid, citric acid, tartaric acid and malic acid. And formic acid was confirmed in the red clover, orchard grass and brome grass as volatile organic acid. In consideration of the results mentioned in above the effects of excrements and components of roots found in this studies may be summarized as follows. 1) The red clover generally gave a disadvantageous effect on the Graminaceae. Such trend was considered chiefly caused by the presence of many organic acids, namely oxalic, citric, tartaric, malonic, malic, succinic and formic acid. 2) The orchard grass generally gave an advantageous effect on the Leguminosae. This may be due to a few kinds of organic acid contained in the root, namely oxalic, citric, tartaric, malic and formic acid. Furthermore a certain of promotive materials for growth was noted. 3) As long as the root of brome grass are not rotten, it gave a disadvantageous effect on the Leguminosae and Graminaceae. This may be due to the fact that several unidentified volatile organic acid were also included besides the confirmed organic acid, namely oxalic, citric, tartaric, malic and formic acid. 5. Effects of Components in Roots to the Soil Sickness : 1) It was considered that the cause of alleged red clover's soil sickness did not result from the toxic components of the roots. 2) It was recognized that the toxic components of roots might be the cause of soil sickness in case the orchard grass and brome grass were put into the long-term single cropping. 6. Effects of Rooted Components to the Companion Crops in the Cropping System : a) In case of aqueous extracts of fresh roots and aqueous excrements (Inter cropping and mixed cropping) : 1) Advantageous combinations : Orchard grass->Red clover, Soybean, Italian ryegrass->Red clover, 2) Disadvantageous combinations : Red clover->Ladino clover, Lespedeza, Orchard grass, Italian ryegrass, Fescue Ky-31, Brome grass, Barley, Wheat, Corn and Hog.millet, Orchard grass->Lespedeza, Orchard grass, Barley and Hog-millet, Brome grass->Red clover, Ladino clover, Lespedeza, Soybean, Orchard grass, Brome grass, Barley, Wheat, Sorghum, Corn and Hog-millet, 3) Harmless combinations : Red clover->Red clover, Soybean and Sorghum, Orchard grass->Ladino clover, Italian ryegrass, Brome grass, Wheat, Sorghum and Corn, Brome grass->Italian ryegrass, b) In case of aquecus extracts of rotting roots(After cropping) : 1) Advantageous combinations : Red clover->Red clover and Sorghum, Orchard grass->Red clover, Ladino clover, Soybean, Sorghum, and Corn, Brome grass->Red clover, Soybean and Sorghum, 2) Disadvantageous combinations : Red clover->Lespedeza, Orchard grass, Italian ryegrass, Brome grass, Barley, Wheat, and Hog-millet Orchard grass->Barley and Hog-millet, Brome grass->Orchard grass, Brome grass, Barley and Hog-millet, 3) Harmless combinations : Red clover->Ladino clover, Soybean and Corn, Orchard grass->Lespedeza, Orchard grass, Italian ryegrass, Brome grass and Wheat Brome gass->Ladino clover, Lespedeza, Italian ryegrass and Wheat.

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Leaky wave antenna analysis design, and implementation (누설파 안테나 해석 설계 및 제작)

  • 홍재표;조웅희;이종익;윤리호;이정형;조영기;엄효준
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.11
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    • pp.88-96
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    • 1996
  • Periodically slotted dielectricalloy filled parallel-plate waveguide as a leaky wave antenna is designed and fabricated at the center frequency of 10.0GHz. The antenna was fed by use of a hog-horn structure. The hog-horn and the two side walls and the lower plate of parallel-plate waveguide were fabricated form duralumin. The upper plate of parallel-plate waveguide with 48 periodic slots was made of copper plate of 1mm thickness. The dielectric material inside the parallel-plate waveguide was chosen to be paraffin. The experimental radiation pattern for the fbricated antenna was compared with the theoretical results for the finite periodic structure.

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Evaluation of Continuously and Intermittently Aerated Hog Manure Compost Stability in a Pilot-scale Bin Composting System (파일럿 규모 빈 퇴비화 시스템에서 연속 및 간헐 통기 돈분 퇴비의 안정도 평가)

  • Hong, Ji-Hyung
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.40 no.5
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    • pp.100-108
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    • 1998
  • Compost stability represents the state of microbiological activity and measurements of respiration either through $CO_2$ evolution or $O_2$ uptake should provide the best indication of this state. Hog manure amended with sawdust was composted in a pilot-scale reactor vessels using continuous and intermittent aeration for 3 weeks. In this study we evaluated the $CO_2$ respiration rate effect of aeration method on the reduction of $CO_2$ evolution, and investigated the stability of fresh and finished compost for plant growth. The intermittently aerated composting is a practical proposition for a very stable compost making. The $CO_2$ respiration rate in the fresh and finished compost during intermittently aerated composting was maintained from 0.3 to 1.4 and was good for use in horticulture, while the continuously aerated composting was 7 to 23 and needed more time for compost curing.

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