• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.8 no.1
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• pp.95-104
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• 1998

This Study was conducted at nine Histological Laboratories in the hospitals located in Seoul from August 20 to October 10, 1996. The main purpose of this study was to assess the TWA & STEL exposure to formaldehyde and to evaluate factors affecting formaldehyde concentration in Histological Laboratory. With evaluated factors, proper improvement strategy to minimize exposure was suggested. The results are summarized as follows ; 1. The TWA concentrations of the personal samples ranged from 0.02 to 3.86 ppm and that of one laboratory out of nine was exceeding 0.75 ppm, USA OSHA standard. The TWA concentrations of the area samples ranged from 0.08 ppm to 1.49 ppm and those of three laboratories out of nine were exceeding OSHA standard. 2. When measurement was conducted on the working tables. around Formaldehyde vessel, fixation container and storage cabinet, the geometric means of TWA concentrations were 0.62 ppm, 0.64 ppm. 0.58 ppm and 0.37 ppm respectively. 3. When overnight fixed specimens were examined, the STEL concentrations of personal samples ranged from 0.59 ppm to 5.01 ppm, those of three laboratories out of nine were exceeding 2 ppm, OSHA standard and the STEL concentrations of area samples ranged from 0.31 ppm to 3.08 ppm, those of four laboratories from nine were exceeding OSHA standard. 4. Factors affecting Formaldehyde exposure level were sanitation state of laboratory, volume of the room and opening state of fixation container & formaldehyde vessel(p<0.05).

• The korean journal of orthodontics
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• v.48 no.6
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• pp.405-411
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• 2018

Objective: This study was performed to investigate the rate of tooth movement and histological characteristics of extraction sockets those were subjected to corticotomy. Methods: A split-mouth randomized controlled trial experiment was designed. Thirty-two adult, male Wistar rats were divided into 2 groups: healing extraction socket (H) and recent extraction socket (R); these groups were randomly classified into 4 subgroups (0/7/21/60 days). The first maxillary molar was extracted on 1 side and 2 months were allowed for complete bone healing; then, the corresponding molar was extracted on the other side and surgical intervention was performed at the mid-alveolar point of the first maxillary molar. Ten grams of continuous force was applied. The outcomes measured were rate of tooth movement, percentage of periodontal space and histological evaluation. The rate of tooth movement was calculated as the measured distance divided by the duration of molar movement. Histomorphometric evaluations were performed on the second and third maxillary molars. The Wilcoxon signed rank test was used to compare differences between the two groups. Results: There were no significant differences in the rates of tooth movement between H and R groups at any of the 4 time points. The histological appearance and percentage of periodontal space between the R and H groups also demonstrated no significant differences. Conclusions: The rates of orthodontic tooth movement into recent and healed socket sites did not differ between the groups. Histological analysis of tooth movement revealed regional acceleration during every time period.

• Biomedical Science Letters
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• v.13 no.2
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• pp.111-117
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• 2007

Temporary occlusion of the parent artery or feeding artery is an useful method in microsurgery for cerebrovascular diseases. The advantages of the temporary clipping for intracranial aneurysm surgery have already been proven by many experimental and clinical reports. Currently, there are two methods of temporary clipping: 1) intermittent clipping, 2) continuous clipping. In many previous studies, the intermittent, repeated clipping technique was reported to reduce ischemic damage to the brain, but it is still debated. On the other hand, a comparison of the histological changes on the arterial wall between each clipping method has not been sufficiently reported yet. So the authors performed experimental temporary clipping on the common carotid and femoral arteries of about 25 rats using the Sugita temporary mini-clip. The specimens were divided into two major groups and seven subgroups: Group I (I-1, I-2, I-3, I-4, intermittent clippings for 5 minutes were done once, twice, three times, and few times), and Group C (C-10, C-15, C-20, continuous clippings for 10, 15, 20 minutes, respectively). The reperfusion time after the temporary clipping was the same as the clipping duration. Under light microscope, the histological findings by Hematoxylin-Eosin staining were examined in all specimens, which were obtained at each time interval after temporary clipping. Then the histological changes of the arterial walls by two different methods were compared with the normal specimen. The results suggest that intermittent temporary clipping is less damaging on the arterial wall than single continuous clipping.

• The Korean Journal of Cytopathology
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• v.18 no.1
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• pp.20-28
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• 2007

This study was performed to compare Surepath$^{TM}$ liquid-based smear and a conventional cervicovaginal smear with reference to a histological diagnosis. A hybrid capture test (HCII) was also performed and analyzed. We collected matched cases for cervicovaginal cytology-histology: 207 cases for conventional cytology (CC) and 199 cases for liquid-based cytology (LBC). HCII was performed in 254 patients. When a cytological diagnosis of ASCUS or above (ASCUS+) is classified as positive and a histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for LBC was 91.7% and 75.9%, respectively and the sensitivity and specificity for CC was 62.6% and 96.1%, respectively. When a cytological and histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for LBC was 77.5 and 96.6%, respectively and the sensitivity and specificity for CC was 49.7% and 100%, respectively. When a histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for HCII was 78.9% and 78.1%, respectively. The concordance ratio between the cytological and histological diagnosis was 80.4% (kappa=76.0) for LBC and 56.5% (kappa=55.1) for CC. LBC is more sensitive and less specific then CC, as a cytological cutoff level of ASCUS, but more sensitive and equally specific, as a cytological cutoff level LSIL or HSIL. LBC is more reliable with a high concordance ratio between the cytological and histological diagnosis.

• Archives of Plastic Surgery
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• v.37 no.5
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• pp.535-546
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• 2010

Purpose: The subcutaneous fat tissue is separated into 2 layers by the subcutaneous fascia: the superficial and deep fat layers. The two fat layers have different structures according to the body regions. The purpose of this study is to evaluate the distribution and pattern of the two fat layers in the human body by computed tomography (CT) and histological analysis according to age, sex, anatomical region, and body mass index (BMI). Methods: This study included 200 males and 200 females who underwent 64-channel dynamic CT in our hospital. The patients were divided into 5 groups according to 10 years of their ages separately in either male or female gender. The thickness of the superficial and deep fat layers was measured in the abdominal, pelvic, and femoral regions, and we analyzed the values. Statistical analyses were performed using SPSS software. The $3{\times}3$-cm whole fat layers were harvested from the same sites of 3 cadavers for histological examination, and one cadaver was dissected for gross evaluation. Results: The total thickness of subcutaneous fat tissue was greater in females than in males, and the ratio of the superficial fat layer to the whole fat layer was higher in females. The superficial fat layer became thinner with increasing age in males. As BMI increased, the total fat layer became thicker, and the superficial fat layer became thicker than the deep fat layer. On histological examination, the superficial fat layer had small adipose lobules and showed a densely distributed pattern in the abdominal region, whereas in the femoral region, it had large adipose lobules and showed a sparsely distributed pattern. There were no significant differences in the histological findings of the deep fat layer between the 3 body regions. Conclusion: Significant differences in histological findings of the two fat layers were found in relation to age, sex, anatomical region, and BMI. The superficial fat layer became thinner with increasing age in males, but it was constant in females. As BMI increased, the total thickness of subcutaneous fat tissue became greater, and the superficial fat layer became thicker than the deep fat layer. Our measurements can be used to understand the characteristics of the fat layers in relation to age, sex, anatomical region, and BMI.

• Journal of the Korean Academy of Clinical Electrophysiology
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• v.6 no.1
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• pp.1-15
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• 2008

This study was designed to investigate the effect of treadmill exercise of low-intensity and high-intensity on the functional recovery and histological change in spinal cord injury rats. Sprague-Dawley rats were experimented(n=15) for this research. Spinal cord injury was induced by the NYU drop impacter device after laminectomy. After operation, rats were test at modified Tarlov scale at 3 days, and divided into the control group(n=5), experimental group I(n=5, low-intensity treadmill) and experimental group II(n=5, high-intensity treadmill). The rats were disciplined from 7 day through 21 day. Functional recovery was evaluated by the BBB scales and the Grid Walk test for the progressive locomotor recovery at 3, 7, 14, 21 days. Histopathological studies for the muscle in order to observation the change of damage and size of the organized surface which is visible visually it executed hematoxylin & eosin stain. According to the result of 4 weeks of treadmill exercise, group II showed improvement than group I of motor behavior after spinal cord injury.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.31 no.2
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• pp.150-160
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• 2005

Background: For normalization of displaced anatomical structure by large cyst, two-step procedure (decompression and enucleation afterward) has been recommended. However, the histological transformation after cystotomy for decompression was shown frequently in secondary enucleation. Therefore, analyses about effects and histological changes after decompression have been necessary. Methods: 48 cases diagnosed as large odontogenic cyst in the jaw and treated by decompression and secondary enucleation were retrospectively analyzed in clinical, rediographical, and histological aspects. Results: In dentigerous cyst, decompression was much useful. Impacted permanent teeth were erupted and reduction rate was higher than that of odontogenic keratocyst (OKC) and apical periodontal cyst. In OKC, among the 29 cases, 11 cases showed no-keratosis, proliferation and rete-ridge elongation after decompression. 4 cases showed no-keratosis, only. 7 cases showed orthokeratosis and rete-ridge elongation and 6 cases showed reteridge elongation, only. 1 case had no change. And the recurrence rate for OKCs was 10.3%. For all odontogenic cysts in this study, dysplasia was not found in cystic lining after decompression. Conclusions: This study implied that decompression for large odontogenic cyst was useful treatment modality because it was conservative treatment and recurrence rate was low although long treatment period was required.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.17 no.1
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• pp.75-81
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• 2004

Objective: The study is performed to assure the effect of Sochungyong-tang against the wide-spread syndrome allergic rhinitis. For this purpose, the Sochungyong-tang is administered to the rat, which was induced the allergic rhinitis, then the histological change is observed. And the change of the number of neutrophil and eosinophil is also observed. Methods: The Sprague-Dawley male rats, about 200g of body weight, by Saemtako BIO Korea were divided into normal, control, and experimental group. The rats were adapted under the constant temperature and humidity for a week. The solid feed and the water were provided ad lid during the whole experiment By the Levine & Vaz method, the allergic reaction induced with ovalbumin as antigen by intraperitoneal route. As the Sochungyong-tang is administered, the histological changes of neutrophil, eodinophil, and mucosal findings were observed. Results: 1. Number of neutrophil was significantly decreased in the experimental group as compared with the control group. 2. Number of eosinophil was significantly decreased in the experimental group as compared with the control group. 3. The mucosa and submucosa of the nose in experimental group were appeared as normal histological findings. Conclusion: According to the above results, it is supposed that the Sochungyong -tang has considerable effect on allergic rhinitis.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2385-2390
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• 2015

Background: Invasive ductal (IDC) and lobular (ILC) carcinomas are the common histological types of breast carcinoma which are difficult to distinguish when poorly differentiated. Discoidin domain receptor (DDR1) and Drosophila dishevelled protein (DVL1) were recently suggested to differentiate IDC from ILC. Objectives: To assess the expression of DDR1 and DVL1 and their association with histological type, grading and hormonal status of IDC and ILC. Materials and Methods: This cross sectional study was conducted on IDC and ILC breast tumours. Tumours were immunohistochemically stained for (DDR1) and (DVL1) as well as estrogen receptor (ER), progesterone receptor (PR) and C-erbB2 receptor. Demographic data including age and ethnicity were obtained from patient records. Results: A total of 51 cases (30 IDCs and 21 ILCs) were assessed. DDR1 and DVL1 expression was not significantly associated with histological type (p=0.57 and p=0.66 respectively). There was no association between DDR1 and DVL1 expression and tumour grade (p=0.32 and p=1.00 respectively), ER (p=0.62 and 0.50 respectively), PR (p=0.38 and p=0.63 respectively) and C-erbB2 expression (p=0.19 and p=0.33 respectively) in IDC. There was no association between DDR1 and DVL1 expression and tumour grade (p=0.52 and p=0.33 respectively), ER (p=0.06 and p=0.76 respectively), PR (p=0.61 and p=0.43 respectively) and C-erbB2 expression (p=0.58 and p=0.76 respectively) in ILC. Conclusions: This study revealed that DDR1 and DVL1 are present in both IDC and ILC regardless of the tumour differentiation. More studies are needed to assess the potential of these two proteins in distinguishing IDC from ILC in breast tumours.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.665-678
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• 1994

This study was undertaken to investigate histological changes according to the radiosensitivity in the spermatogenic cells in Korean native pheasants. During spermatogenetic period, testes wete collected from male adult Korean native pheasant and they were used as experimental and control birds. The experimental group was divided into a single-dose whole body irradiation group(400, 600, 800 and 1000 rads) and a split-dose whole body irradiation groups(400/2, 600/2, 800/2 and 1000/2 rads). A Henseky's $^{60}Co$ ${\gamma}$-radiotherapy machine was used for this experiment and the dose rate of $^{60}Co$ ${\gamma}$-ray was 104 rads/min. The experimental birds were sacrificed at 24 and 72 hrs after irradiation and the control pheasants were sacrificed at the same time. General histological changes of seminiferous epithelial cells were observed by hematoxylin-eosin stain with light microscope. The results obtained are summarized as follows ; 1. In the single-dose and the split-dose irradiation groups, the average diameter of the seminiferous tubule was decreased compared with control group. 2. Seminiferous epithelial cells were more severely damaged after 72 hrs than after 24 hrs of single-dose irradiation of 400, 600 and 800 rads but the difference of cell injury was almost not observable with the elapsed time in the group of the single-dose irradiation of 1000 rads. 3. The damage of spermatogenic cells were more severe after 24 hrs than after 72 hrs of the split-dose irradiation of 400 rads but the split-dose irradiation of 600, 800 and 1000 rads were more severe after 72 hrs than after 24 hrs.