• Title/Summary/Keyword: Histochemisty

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Ultrastructure and Histochemisty of Deteminate Vascular Cambium in Rhizome of Botrycnium tematum (고사리삼 지하경에서 유한 유관속 형성층의 미세구조 및 조직화학)

  • 소웅영
    • Journal of Plant Biology
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    • v.38 no.1
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    • pp.115-119
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    • 1995
  • In order to clarify the relationship between the activity and age of vacular cambium in the rhizome of Botrychium ternatum, the cambia of the 6th and 10th internodes from its shoot apex were compared ultrastructurally and histochemically. The cambium in the 6th internode had two to four radially arranged cells in each row, and the cells are radially flattened and had thiner tangential walls than radial. The cambial cells were ultrastructurally characterlized by the presence of a large vacuole, few storage materials such as starch grains and lipid droplets, and various cell organelles such as mitochondria, endoplasmic reticulums and dictyosomes in cytoplasm. On the other hand, the cells of cambium in the 10th internode showed irregular arangement and somewhat enlargement in radial plane. The cells were filled with numerous storage materials and small vacuoles and had few cell organelles except for mitochondria. In addition, the activity of acid phosphatase appeared in the cambium of the 6th internode but of the 10th. From the above results, it is clear that the vascular cambium of the 6th internode was active and that of the 10th was dormant. Thus we reached a conclusion that the rhizome of '||'&'||'trychium ternatum has determinate cambium.

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Histochemical Study on Glycosaminoglycans of Esophageal Mucous Cells in Agramus agramus, Inimicus japonicus, Epinephelus chlorostigma and Helico-lenus dactylopterus

  • Jo, Un-Bock;Jung, Kwun-Soon;Hong, Mal-Sook;Jo, Gi-Jin
    • Journal of Life Science
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    • v.10 no.2
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    • pp.1-8
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    • 2000
  • This experiment was performed to study the structure of esophageal mucousa and the histochemical properties of glycosaminoglycans of esophageal mucous cells in four teleostean species, i.e., Agramus agramus, Inimicus japonicus, Epinephelus chlorostigma and Helicolenus dactylopterus. To observe the structure of eosphageal mucosa, hematoxylin-eosin(H-E) staining was used. The glycosaminoglycans was stained with PAS(periodic acid schiff), alcain blue(AB) pH 2.5, AB pH 1.0, aldehyde fuchsin(AF) pH 1.7, AF pH 1.0, AB pH 2.5-PAS, AB pH 1.0-PAS and AF pH 1.7-alcian blue pH 2.5. As for the amount and histochemical composition of glycosaminglycans in Agramus agramus, most of mucous secreting columnar cell and mucous cells contain large and moderate amount of neutral glycosaminoglycans. A few of mucous cells having small amount of neutral glycosamino-glycans and minimal amount of sulfated glycosamino-glycans. In Inimicus japonicus, the esophageal mucous cells were composed of most of medium sized and large mucous cells with moderate amount of neutral glycosaminoglycanonly only, a few of medium sized and large mucous cells and most of small mucous cells with considerable amount of neutral glycosaminoglycans and minimal to small amount of nonsulfated glycosaminoglycans, and a feq of small mucous cells with small amount of neutral glycosaminoglycans and minimal amount of sulfated glycosaminoglycans. In Epinephelus chlorostigma, most of medium sized and large mucous cells were mixed small amount of neutral glycosaminoglycans with sulfated glycosaminoglycans, a few of which were contained with moderate or considerable amount of neutral glycosaminoglycans with sulfated glycosaminoglycans, while most of small mucous cells containing considerable amount of neutral glycosaminoglycans and small to moderate or considerable to minimal amount of nonsulfated glycosaminoglycans(sialomucin) a few of which containing a mixture of considerable amount of neutral glycosaminoglycans and considerable amount of nonsulfated glycosaminoglycans(sialomucin)only. In Helicolenus dactylopterus, most of medium sized and large mucous cells, mixing with moderate to considerable amount of neutral glycosaminoglycans and small to moderate amount of sulfated glycosaminoglycans, while most of small mucous cells with considerable amount of nonsulfated glycosaminoglycans (sialomucin), a few of which having a mixture of considerable amount of neutral glycosaminoglycans and considerable or small amount of nonsulfated glycosaminoglycans(sialomucin).

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