• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1104-1110
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• 2006

Recombinant antibody-toxin is a bifunctional protein that binds and kills a target cell expressing a specific antigen on the surface of the cell, and its structure is chimeric, in which a toxin is fused to an antigen-binding domain such as scFv or Fab. Divalent antibody-toxin molecules showed higher cytotoxicities against cancer cell lines than monovalent molecules. However, the yields of the divalent molecules were very low. In this study, we introduced the CH2, CH3, or CH2-CH3 (=Fc) domain of antibody in the middle of the Fab-toxin between the hinge region of human IgG1 and the toxin domain to increase the yield. The covalently bonded dimer could be formed by three disulfide bridges from cysteine residues in the hinge region. The molecule with the CH3 domain showed about 3-fold higher dimerization yield than previously constructed Fab-toxin molecules, while maintaining the cytotoxic activity comparable to that of scFv-toxin. However, the introduction of CH2 or Fc domain to the same position showed little effect on the dimerization yield. We also observed that the introduction of the CH3 region made it possible to form noncovalently associated dimer molecules.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.04a
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• pp.259-266
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• 2000

This study was conducted to investigate the seismic behavior assessment of circular reinforcement concrete bridge piers particularly with regard to assessing the displacement ductility curvature ductility response modification factor(R) and plastic hinge region etc, The experimental variables of bridge piers test consisted of transverse steel details amount and spacing different axial load levels etc. The test results indicated that reinforcement concrete bridge piers with confinement steel by the code specification exhibited suffcient ductile behavior and seismic performance. Also it is found that current seismic design code specification of confinement steel requirements may be revised.

• Proceedings of the Korea Concrete Institute Conference
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• 2000.10a
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• pp.583-588
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• 2000

While a great progress has been achieved in predicting the ultimate shear strength in the RC members, it is a fact that a method in order to estimate the ductility of RC members still has to be looked for. This study theoretically predict the ductility of RC beams which fail in shear after flexural yielding by considering the deterioration of concrete strength in plastic hinge region.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.11a
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• pp.617-620
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• 2003

This paper presents the confinement effect of transverse reinforcement and cross-tie in hollow rectangular sectional columns. 20 analytical models with different amounts of transverse reinforcement and cross-tie in a plastic hinge region were analyzed by 3D nonlinear FEM. The analytical results show that the higher ductility can be achieved by the resonable combination of transverse re-bar and cross-tie providing sufficient lateral confinement.

• Proceedings of the Korean Biophysical Society Conference
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• 1997.07a
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• pp.9-10
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• 1997

Calmodulin: very large conformation change of helix uncoiling, hinge-bending and domain rotation. Calmodulin (CaM) is the principal Ca$\^$2＋/ -dependent regulator of a variety of important eukaryotic cellular processes. In many of these processes, calmodulin activates a plethora of target enzymes, and the calmodulin-binding domains in several targets have been shown to residue in a region of about 18-residue peptide segment.(omitted)

• Journal of Microbiology and Biotechnology
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• v.2 no.1
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• pp.56-65
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• 1992

The complete nucleotide sequence of the Bacillus circulans F-2 RSDA gene, coding for raw starch digesting a-amylase (RSDA), has been determined. The RSDA structure gene consists of an open reading frame of 2508 bp. Six bp upstream of the translational start codon of the RSDA is a typical gram-positive Shine-Dalgarno sequence and the RSDA encodes a preprotein of 836 amino acids with an Mr of 96, 727. The gene was expressed from its own regulatory region in E. coli and two putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. Confirmation of the nucleotide sequence was obtained and the signal peptide cleavage site was identified by comparing the predicted amino acid sequence with that derived by N-terminal analysis of the purified RSDA. The deduced N-terminal region of the RSDA conforms to the general pattern for the signal peptides of secreted prokaryotic proteins. The complete amino acid sequence was deduced and homology with other enzymes was compared. The results suggested that the Thr-Ser-rich hinge region and the non-catalytic domain are necessary for efficient adsorption onto raw substrates, and the catalytic domain (60 kDa) is necessary for the hydrolysis of substrates, as suggested in previous studies (8, 9).

• Computers and Concrete
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• v.17 no.3
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• pp.387-406
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• 2016

Confined transverse reinforcement was arranged in a plastic hinge region to resist the lateral load that increased the lateral confinement effect in the bridge substructure. Columns increased the seismic performance through securing stiffness and ductility. The calculation method of transverse reinforcements at plastic hinges is reported in the AASHTO-LRFD specification. This specification was only proposed for solid reinforced concrete (RC) columns. Therefore, if this specification is applied for another column as composite column besides the solid RC column, the column cannot be properly evaluated. The application of this specification is particularly limited for composite hollow RC columns. The composite hollow RC column consists of transverse, longitudinal reinforcements, cover concrete, core concrete, and an inner tube inserted in the hollow face. It increases the ductility, strength, and stiffness in composite hollow RC columns. This paper proposes a modified equation for economics and rational design through investigation of displacement ductility when applying the existing specifications at the composite hollow RC column. Moreover, a parametric study was performed to evaluate the detailed behavior. Using these results, a calculation method of economic transverse reinforcements is proposed.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.261-264
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• 2004

This paper presents the design and test of elliptical vibration assisted cutting tool post. It is actuated by two piezoelectric actuators which are connected to the moving part through the elastic hinge with its role of imposing the preliminary pressures. These two actuators are located at right angles so that the resulting tool tip moves like a two-dimensional ellipse. Also, the tool post is activated within the region of linear actuation in order to overcome the distorted elliptical motion. For the precise measurement of the displacement of the tool tip, three-dimensional experimental apparatus was designed and the strokes of the tool post in major and minor axes were measured. The results show that the tool post can produce the variety of vibration locus from a circle with a radius of 5 ${\mu}{\textrm}{m}$ to an ellipse with a major axis, a =10 ${\mu}{\textrm}{m}$, and a minor axis, b =2.5 ${\mu}{\textrm}{m}$

• Journal of Korean Society of Steel Construction
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• v.18 no.6
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• pp.687-696
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• 2006

An efficient numerical method is developed to estimate the elasto-plastic post-buckling strength of space-framed structures. The inelastic ultimate strength of beam-columns and frames is evaluated by the parametric study. Applying the improved plastic hinge analysis that evaluate the gradual stiffness decrease effects due to spread of plasticity, elasto-plastic post-buckling behavior of steel frames is investigated considering the various residual stress distributions. Introducing the plastification parameter that represent pread of plasticity in the element and performing parametric study of equivalent element force and member idealization, finite-element solutions for the elasto-plastic analysis of space frames are compared with the results by plastic region analysis, shell elements and experimental results.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.3
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• pp.79-88
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• 2003

For the construction of PC bridge piers the implementation of 1992 seismic provisions, longitudinal steels were practically lap-spliced in the plastic hinge region. Experimental investigation was conducted ductility of evaluate the seismic earthquake-experienced reinforced concrete columns with 2,5 aspect ratio. Six test specimens were mode with test parameters: confinement ratios, lap splices, and retrofitting FRP materials. They were damaged under series of artificial earthquakes that could be compatible in Korean peninsula. Directly after the pseudo-dynamic test, damaged columns were retested under inelastic reversal cyclic loading simultaneously under a constant axial load, P＝0.1f$\_$ck/A$\_$g/. Residual seismic performance of damaged columns was evaluated and compared to that of the corresponding original columns. Test results show that PC bridge piers with lap-spliced longitudinal steels appeared to fail at low ductility. This was due to the debonding of the lap splice, which resulted from insufficient development of the longitudinal steels. The specimens externally wrapped with composite FRP straps in the potential plastic hinge region indicated significant improvement both in flexural strength and displacement ductility.