• Title/Summary/Keyword: Higher Plants

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Genotoxicity of the Herbicide 2,4-Dichlorophenoxyacetic acid (2,4-D): Higher Plants as Monitoring Systems

  • Enan, Mohamed R.
    • Journal of Forest and Environmental Science
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    • v.25 no.3
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    • pp.147-155
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    • 2009
  • Higher plants provide valuable genetic assay systems for screening and monitoring environmental pollutants. They are now recognized as excellent indicators of mutagenic effects of environmental chemicals and are applicable for the detection of environmental mutagens both indoor and outdoor. 2,4-dichlorophenoxyacetic acid (2,4-D) is a herbicide commonly used in agriculture. The residues of 2,4-D are present in air, water, soil and edible plants. It constitutes a real hazard to the public health because it's wide spread use in agriculture. Genotoxic effects of 2,4-D on plant cells and potential of higher plants as a biomonitoring system for detecting chemical mutagens are evaluated. It is recommended that higher plant systems have been accepted by regulatory authorities as an alternative biomonitoring system for the detection of possible genetic damage resulting from pollution and the use of environmental chemicals.

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Comparison of Trace Element, Metal, and Metalloid Contents in North and South Korean Plants

  • Park, Jeong-Soo;Chung, Ha-Sook;Lee, Eun-Ju
    • Journal of Environmental Science International
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    • v.23 no.6
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    • pp.995-1001
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    • 2014
  • When relations improve between North and South Korea, there will be demand for North Korean edible plants because of the low labor cost and similar environmental conditions. However, there is no reliable information about trace elements, metals, and metalloids in edible plants from North Korea. Selenium (Se) and germanium (Ge) have positive effects on basic human health and are therapeutical in diverse illnesses. Metal and metalloid (Cd, Pb) poisoning, on the other hand, can cause many health problems. Plants collected from North Korea had higher selenium content than those from South Korea. Although none of the collected species exceeded the permissible levels of cadmium and lead, their content in plants was significantly higher in North Korea than in South Korea. The high metal contents in plants collected from North Korea may be associated with the soil physicochemical properties as well as the accumulated amounts of elements in the soil.

Physiological and Biochemical Changes in Lucerne (Medicago sativa) Plants Infected with 'Candidatus Phytoplasma australasia'-Related Strain (16SrII-D Subgroup)

  • Ayvaci, Humeyra;Guldur, M. Ertugrul;Dikilitas, Murat
    • The Plant Pathology Journal
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    • v.38 no.2
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    • pp.146-158
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    • 2022
  • Changes in physiological and biochemical patterns in lucerne plants caused by the presence of 'Candidatus Phytoplasma australasia', which is one of the significant pathogens causing yield losses in lucerne plants, were investigated. Significant differences were evident in total chlorophyll, chlorophyll a, chlorophyll b, and protein amounts between 'Ca. Phytoplasma australasia'-positive and negative lucerne plants. Stress-related metabolites such as phenol, malondialdehyde, and proline accumulations in 'Ca. Phytoplasma australasia'-positive plants were remarkably higher than those of phytoplasma-negative plants. As a response to disease attack, phytoplasma-positive plants exhibited higher antioxidant enzymes (peroxidase and catalase) and nonenzymatic metabolite responses such as jasmonic and salicylic acids. We state that partial disease responses were revealed for the first time to breed resistant lucerne lines infected by 'Ca. Phytoplasma australasia'.

Improvement of Drought Tolerance in Transgenic Tobacco Plant (형질전환 담배의 내건성 개선)

  • Park, Yong Mok
    • Journal of Environmental Science International
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    • v.25 no.1
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    • pp.173-179
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    • 2016
  • Leaf water and osmotic potential, chlorophyll content, photosynthetic rate, and electrolyte leakage were measured to evaluate tolerance to water stress in wild-type (WT) and transgenic tobacco plants (TR) expressing copper/zink superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts. Leaf water potential of both WT and TR plants decreased similarly under water stress condition. However, leaf osmotic potential of TR plants more negatively decreased in the process of dehydration, compared with WT plants, suggesting osmotic adjustment. Stomatal conductance (Gs) in WT plants markedly decreased from the Day 4 after withholding water, while that in TR plants retained relatively high values. Relatively low chlorophyll content and photosynthetic rate under water stress were shown in WT plants since $4^{th}$ day after treatment. In particular, damage indicated by electrolyte leakage during water stress was higher in WT plants than in TR plants. On the other hand, SOD and APX activity was remarkably higher in TR plants. These results indicate that transgenic tobacco plants expressing copper/zink superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts improve tolerance to water stress.

Studies on the Root Development of the Rice Plants (Oryza sativa L.) in Accordance with Salt- diminution at the Saline Paddy Field (간척지의 숙답화에 따른 수도근군형성에 관한 연구)

  • 정원일
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.3
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    • pp.299-304
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    • 1983
  • It has been ascertained by a few researchers that soil conditions under which the rice plants were cultivated have some effects upon the root formation of the rice plants. But, much is not known about the root formation of the rice plants cultivated in the saline paddy fields. The goal of the present investigation is to study morphological effects of the soil salinity on the development of the rice root system. The following results were obtained: 1. Under the conditions of higher soil salinity, root systems developed well at surface soil, however, root systems developed well and distributed evenly through surface and sub-soil at the saline fields where soil salinity was lower. 2. The rice plants cultivated in the higher soil salinity form less crown roots than the rice plants which cultivated at the lower soil salinity. 3. As for the formation of the stunted roots, it was found out that relatively rice plant cultivated in higher soil salinity forms more stunted roots than the rice plants cultivated in lower soil salinity. 4. The crown root cultivated in the higher soil salinity forms more lateral roots per unit langth than the root cultivated in lower soil salinity. 5. As for the root hair formation, the crown root cultivated in higher soil salinity bears less haired epidermis and shorter root hairs than the root cultivated in lower soil salinity.

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Analysis of Preference of Environmental Image for the Increase and Promotion of Rose Consumption

  • Jeong, Sun Jin;Gim, Gyung Mee;Kim, Jae Soon;Jang, Hye Sook;Lee, Geun Woo
    • Journal of People, Plants, and Environment
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    • v.22 no.1
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    • pp.53-63
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    • 2019
  • The purpose of this study was to investigate the preference of plant environmental images for flower plant consumption. This study came up with a total of four treatments including one image without plants and three images with flower plants (three roses, rose gift, rose garden). We conducted a survey on 104 men and women through the Google (online) survey and analyzed the data. The preference was higher with statistical significance for environmental images with plants than the image without plants. The preference for environmental images of roses was highest in the order of rose gift > rose garden > three roses. As a result of the cross-tabulation analysis, it was found that there was a significant difference in the preference for environmental images of roses according to general characteristics such as educational background and residence type. In terms of educational background, 2-year college graduates showed higher preference for the three environmental images of roses with statistical significance compared to high school graduates, university graduates and masters or higher. As a result of determining the difference in preference according to residential types, residents of multiplex houses showed higher preference for the "rose garden" environment image than residents of detached houses (p < .05). As a result of examining stress and depression in everyday life, 48.1% (the highest) of the respondents answered that they were "under daily stress" and 48.1% (the highest) of the respondents claimed not to be "under much depression". This study investigated the difference in preference according to demographic characteristics and existence of plants, preference in environmental images using roses, correlation with daily stress and depression, and utility of publicity using photographic images of plants.

Effects of Cadmium on Growth of Arabidopsis thaliana (카드뮴이 애기장대의 생장에 미치는 영향)

  • Park Jong-Bum
    • Journal of Environmental Science International
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    • v.13 no.12
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    • pp.1103-1108
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    • 2004
  • This experiment was carried out to investigate the effects of cadmium on the growth of Arabidopsis thaliana when they were treated with different concentrations of cadmium. The growth of stem was stimulated in the concentrations up to fifty times higher than the official standard concentration of cadmium of pollutant exhaust notified by the Ministry of Environment, but it decreased in the concentration one hundred fifty times or more higher in proportion to the degree of concentration. The growth of root was similar to that of stem, except that the decrease was gradual in the concentration fifty times or more higher. The growth of leaf was almost the same as that of stem, that is, it was stimulated the increase of leaf surface area in the concentration fifty times higher, but decreased in the concentration one hundred fifty times or more higher in proportion to the degree of concentration. The fresh weights of the plants were increased in accord with the degree of growth of the stem and leaf. Concentration of cadmium accumulated in the plants was increased in proportion to the concentration of cadmium. These results show that the growth of plants was stimulated in the soil polluted by cadmium up to fifty times higher than the official standard concentration, but it was decreased in proportion to the degree of concentration in the plants grown in the presence of cadmium more than one hundred fifty times.

Overexpression of Cotton Glutathione S-Transferase (GST) cDNA and Increase of low Temperature and Salt Tolerance in Plants

  • Kang, Won-Hee;Jong Hwa kim;Lim, Jung-Dae;Yu, Chang-Yeon
    • Journal of Plant Biotechnology
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    • v.4 no.3
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    • pp.117-122
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    • 2002
  • Cotton Glutathione S-Transferase(GST: EC 2.5.1.18) was cloned and Gh-5 cDNA was overexpressed in tobacco (Nicotiana tabacum) plants. The transformation of cotton GST in tobacco plant was confirmed by northern blot analysis. Type I and Type II transcript patterns were identified in Gh-5 transgenic tobacco plants. Type I transcripts was only discussed in this paper. Glutathione and 1-chloro-2,4-dinitrobenzene (CDNB) were used as the substrates, and the activity of GST in the type I transgenic plants was about 2.5-fold higher than the non-expressers and wild type tobacco plants. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type I transgenic plants produced functional GST in the cells. Type I showed higher GST specific activity than Type II in the transgenic plants. Control and transgenic seedlings were grown in the growth chamber and under the light at 15$^{\circ}C$, and the effects of cotton GST in the seedlings was evaluated. The growth rate of Gh-5 overexpressors was better than the control and non-transgenic tobacco plants. Salinity tolerance was also analyzed on the seeds of transgenic plants. Seeds of Gh-5 overexpressors and the wild type tobacco seedlings were germinated and grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings at both 50 and 100 mM NaCl solution. But at 0, 150, and 200 mM NaCl concentration, the difference in growth rate was not detected.

Expression of CAB (Chlorophyll a/b Binding Protein) Gene in Transformed Plants (CAB (Chlorophyll a/b Binding Protein) 유전자의 형질전환 식물체에서 발현)

  • 박성원;김선원;이영기;강신웅;이청호;이종철;최순용
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.1
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    • pp.41-45
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    • 2001
  • Transgenic tobacco plants were produced by the transformation of ginseng CAB gene using Agrobacterium tumefaciens LBA4404. The presence of CAB gene in the second generation of transgenic tobacco plant was confirmed by genomic PCR. The photosynthetic ability of transgenic plants was higher than normal tobacco plants and the maximum photosynthetic point of transgenic and normal tobacco plants was 500 $\mu$mol m$^{-2}$ s$^{-1}$ . The photosynthesis of C7, C11, 1, C14 cell lines was higher than normal plants at all the light intensities investigated. The photosynthesis of C2, C11, C14 cell lines in 90% dark condition was higher than normal plants. The chlorophyll contents of transgenic tobacco plants were almost same as normal plants. The % of dry weight, nicotine content, total sugar and nitrogen contents of harvested transgenic tobacco plant leaves were almost same as normal plants.

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