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Differentiation of indigenous balloon flower (Platycodon grandiflorum DC.) germ lines in South Korea by using RAPD analyses (RAPD분석 기술을 이용한 토종도라지의 기원 분석)

  • Kim, Tae-Won;Lee, Soo-Jin;Kim, Man-Bae;Park, Chun-Geon;Shin, Yong-Wook;Cho, Young-Son;Lee, Shin-Woo
    • Journal of Plant Biotechnology
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    • v.41 no.1
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    • pp.19-25
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    • 2014
  • The total production volume has been sharply increased from year 2008 in Gyeongnam province, South Korea by the policy of preservation and promotion of indigenous balloon flower germ lines. In an attempt to assist the Gyeongnam province's policy, in this study, we tried to establish a technique to differentiate the indigenous balloon flower germ lines with those collected within South Korea and China. Our preliminary results indicated that RAPD analyses with five different primers exhibited high frequency of polymorphic DNA bands up to 76.9% and phylogenetic tree indicated that some of the indigenous lines can be easily differentiated with others. However, it was suggested that more advanced techniques such as single nucleotide polymorphic markers need to be developed in particular, by using extra-chromosomal DNA.

Biomass of Primary Producer in the Ch$\check{o}$nsu Bay -Relationships between Phytoplankton Carbon, Cell Number and chlorophyll- (천수만 일차생산자의 생물량 -식물플랑크톤 탄소량과 세포개체수 및 클로로필과의 관계-)

  • Shim, Jae Hyung;Shin, Yoon Keun
    • 한국해양학회지
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    • v.24 no.4
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    • pp.194-205
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    • 1989
  • In order to study the biomass of primary producer, phytoplankton is collected monthly September 1985 to August 1986 in Ch$\check{o}$nsu Bay. Phytoplankton carbon contents which are calculated from phytoplankton volume were ranged from $26.7{\mu}gC/l$ to $960.7{\mu}gC/l$, and average carbon contents of each month lie in the range of $58.6-684.7{\mu}gC/l$(annual mean $208.5{\mu}gC/l$). For net plankton analysis with the carbon contents, cell numbers, and chlorophyll concentrations show a close correlation, while for nanoplankton the correlation was low, indicating that nano-fraction includes a significant portion of picoplankton. Also, the multiple regression analysis with carbon content, cell number, and chlorophyll concentration to size fraction well illustrate the prime importance of the net-fraction in phytoplankton group. C/Chl-a ratios ranged from 9.1 to 100.5, average rations of net- and nanoplankton are 111 and 6.4, respectively. The greater net plankton faction is, the higher C/Chl-a ratio is, however in case of high nanoplankton portion C/Chl-a ratio show low level. These results indicate that the difference of C/Chl-a ratio per phytoplankton cell size be main factor for the variation of C/Chl-a ratio in Ch$\check{o}$nsu Bay. As C/Chl-a ratio fluctuates greatly in coastal ecosystem, that use of a direct conversion of convert chlorophyll to organic carbon may lead erronous estimation.

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Effects of IgY Supplementation on Hanwoo Calves Fed withHigh Quality Roughage (양질 조사료 급여에 따른 한우 송아지의 IgY 처리효과)

  • Hong, Byung-Cheon;Shin, Jong-Suh;Park, Byung-Ki;Kim, Byong-wan;Sung, Kyung-Il;Ahn, Jung-Sang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.2
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    • pp.159-166
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    • 2016
  • This study was conducted to examine the effect of IgY supplementation on growth performance, blood metabolism, and disease occurrence in the weaned calves at 3 or 4 month of age. Average daily gain (ADG) and feed intakes were not affected by IgY supplementation, regardless of weaning months. White blood cell (WBC) count of the control group was higher in the final stage than in the initial stage (p<0.05), while that of the IgY supplemented group was lower in the final stage compared to the initial stage (p<0.05). regardless of treatments. Hematocrit (HCT) and red blood cell (RBC) counts were lower in the final stage than in the initial stage (p<0.05), but mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) counts were higher in the final stage than in the initial stage, regardless of treatments. In the control group, concentrations of albumin and globulin were higher in the final stage compared to the initial stage in the weaned calves at 3 month of age (p<0.05), and concentration of blood urea nitrogen (BUN) was increased in the IgY supplemented group. In the control group, concentrations of aspartate aminotransferase (AST) and gamma (${\gamma}$)-glutamyl transferase (GGT) were higher in the final stage compared to the initial stage (p<0.05). In contrast, concentrations of aspartate aminotransferase (AST) and gamma(${\gamma}$)-glutamyl transferase (GGT) were lower in the final stage compared to the initial stage for the IgY supplemented group. A diarrhea calf was only found in the control group. Manure score was higher in the control group compared to IgY supplemented group (p<0.05). Thus, these results indicates that IgY supplementation had positive effects on some blood metabolites, fecal condition score and diarrhea without negative effect on growth performance of the weaned Hanwoo calves.

Quality Characteristics of Ginger(Zingiber officinale Roscoe) as the Ripening Periods (생강(Zingiber officinale Roscoe)의 숙성기간에 따른 품질특성)

  • Lee, Myung-Hee;Kim, Kyung-Tack;Lee, Kyoung-Hae
    • Journal of Food Hygiene and Safety
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    • v.27 no.4
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    • pp.479-486
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    • 2012
  • This study was conducted to demonstrate quality characteristics of ginger by making aged ginger( AG) with two methods, the frist method was that ginger was aged at constant temperature and humidity chamber for the duration of 10, 20 and 30 days, and the other method was drying the ginger just after steaming it for 3 hours. As the age was being processed, the volume of ginger's appearance decreased rapidly and its color seemed to be darker because of the decrease in moisture. In the case of general components, the content of crude ash was depended on aged periods while the content of crude fat was independent with aged periods, and according to the content of crude protein, there was not any significant differences. The main valuable ingredient which is 6-gingerol showed the decreasing trend as it was exposed to heat with more time, and 6-shogaol which is also one of the main valuable ingredients showed high content at T-II(AG-10days). Free sugar and free amino acid of AG decreased as aged period goes by, and this study found that there were lots of essential amino acid (threonine, glutamic acid, alanine, valine, leucine and tyrosine) in ginger. The amount of unsaturated fatty acid of AG was significantly higher than the amount of saturated fatty acid of AG with the approximate ratio of 60:40, and the amount of free fatty acid of AG did not seem any big differences between AG and none AG. Considering both valuable ingredients and nutritive components, T-I (steamed ginger, SG) and T-II which was aged for 10 days were evaluated excellently.

Dietary Behaviors, Self Perception of Body Image, Hematological Index and Nutrient Intake of Female Athletes in Incheon

  • Cheong, Sun-Hee;Hyuni Sung;Kim, Soon-Ki;Cho, Mi-Hye;Chang, Kyung-Ja
    • Proceedings of the KSCN Conference
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    • 2003.11a
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    • pp.1048-1048
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    • 2003
  • The purpose of this study was to investigate dietary behaviors, body image, hematological index and nutrient intake of female athletes in Incheon. The subjects were 112 female athletes (field and track: n=32, firing: n=27, fencing: n=29, swimming: n=14, badminton: n=10) from middle and high schools in Incheon. This cross-sectional study was conducted by a self-administered questionnaire. Fasting blood samples were obtained and analyzed for hemoglobin (Hb), hematocrit(Hct), ferritin, serum iron, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), unsaturated iron binding capacity (UBC), total iron binding capacity (TIBC) and transferrin saturation (TS). Nutrient intakes collected from 3 day-recalls were analyzed by the Computer Aided Nutritional Analysis Program. Statistical analysis was conducted using SPSS 10.0 program. The results are summarized as follows: Average age of field and track athletes, firing, fencer and swimmer was 14 years and that of badmintoner was 17 years. Most of female athletes had dietary problems such as unbalanced meals, skipping meals, and preference of processed foods. More than 60% of female athletes skipped breakfast. As for perception of body image, most of female athletes perceived themselves fatter compared to normal body image. Especially, field and track athletes were more significantly experienced weight control compared to the other athletes (p<0.05). As for physical burden during exercise, 56.3% of swimmer and 31.3% of field and track athletes answered “very hard”, which showed a significant difference (p<0.001) More than 80% of female athletes had experienced a vertigo during exercise (p<0.01). Also, more than 50% of female athletes except badmintoner had experienced an irregular menstruation (p<0.05). Average serum iron level (p<0.05) and TS (p<0.05) of field and track athletes were significantly lower compared to the other athletes. Serum ferritin of badmintoner, field and track athletes and fencer was significantly lower compared to firing and swimmer (p<0.05). Nutrient intakes of female athletes except vitamin B6, niacin and phosphorus were lower than the Korean RDA. Especially, calcium and iron intakes of female athletes were under the 50% of the Korean RDA. Therefore, proper nutrition education and supplementation are required for female athletes to encourage desirable food habits as well as to improve their nutritional status and exercise performance.

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Diurnal Change in Water Statue of Fruit Tissues During the Growth of Kiwifruit(Actinidia deliciosa) (참다래 과실의 생장에 따른 과실조직의 일중 수분상태 변화)

  • Han Sang Heon
    • Journal of Bio-Environment Control
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    • v.10 no.1
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    • pp.1-9
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    • 2001
  • Fruit growth in kiwifruit shows double sigmoid curve, but it does not certainly indicate as years. Therefore, I though the reason to be easy to the effect of water state change in kiwifruit, investigated diurnal change in water status of fruit tissues with an isoipiestic psychrometers against the fruit growth stage of kiwifruit in 1995 and 1996. Diurnal change in the fruit tissue water potential were little, but violent for fruti growth state III in 1996. The potential of two years dropped gradually approach to harvest time. On the other hand, osmotic potential of the tissues indicated to very similar to water potential, dropped rapidly -1.5MPa before dawn, recovered -1 MPa after 3 h on October 14, were -1~-1.7 MPa at the fruit commercial harvest in 1995. It had a tendency to lower in 1996 than in 1995. It was recorded to the minimum air temperature at the first for an autumn in 1995; 13$^{\circ}C$ from the middle night of October 13 to dawn of October 14. Leaves water potential, which is related to water status of xylem, nearly fell below -1 MPa at before dawn from stage II in 1996. However, it fell so low only at commercial in 1995. At the stage II, osmotic potential and ascent of the turgor pressure was high than 1995-fruit. There parameter suggested that three of kiwifruit in 1996 were status of water stress for stage III. The results from this study indicated that difference of fruit growth between 1995-fruit and 1996-fruit was affected by water status of the fruit tissues, which was influenced by weather condition.

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Development and Lab-scale Plant Study of Coagulation Sedimentation Module using Cyclone (선회류를 이용한 응집침전모듈의 개발 및 실증 연구)

  • Moon, Jinyoung;Cho, Young-Gun;Song, Seung-Jun;Kim, Jin-Han
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.5
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    • pp.3336-3344
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    • 2014
  • The purpose of this study is small scale coagulation module is developed and demonstrated through a lab-scale test. Recent as a sewage treatment rate increases, have heightened the interest in the necessity on the nonpoint source and developing a small processing unit has been increased. Coagulation sedimentation module in this study is additional growth of floc through swirling in the outside zone, reduction of microstructure floc number and the internal settling zone through vertical/level flow complex sedimentation method after the coagulation process precipitation method as an effective high separation efficiency can be divided was also assessed. Coagulation sedimentation module can increase the load factor was 4.4 times compared to conventional clarifier base on the same volume and surface area through vertical/level flow. In this study, this process was selected formation and maintenance of swirling and uniform flow distribution in the internal settling zone as an important design factor, to derive its FLUENT was used to characteristics of the flow model. Through the simulation of swirling, influent velocity, dimensions of external basin, hopper depth of bottom cone was determined and through analysis of velocity distribution, flow distribution detailed specifications are derived like as diameter and number of effluent hole. Lab-scale($120{\ell}/hr$) test results, influent of 300~800 NTU to less than 10 NTU without polymer feeding was able to operate in the 20minutes retention time(surface loading rate $37.3m^3/m^2$-day), and through analysis FLUENT the possibility of using design parameters were derived.

Blood Pressure, Salt Threshold, Salt Preference, Urinary Excretions and Nutrition Knowledge About Blood Pressure of Elementary School Children in Rural Area (농촌 국민학교 아동의 혈압, 짠맛에 대한 역치, 최적 염미도, 뇨 중 배설성분 및 혈압에 관한 영양지식)

  • 김은경
    • Journal of Nutrition and Health
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    • v.26 no.5
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    • pp.625-638
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    • 1993
  • The purpose of this study was to observe the relationship of dietary factors to blood pressure in 5th and 6th grade school children. Salt threshold, salt preference and nutrition knowlege about blood pressure were tested. Twenty-four hour urines were collected for the measurements of the volume and concentrations of sodium, potassium, chloride, calcium, phosphorus, creatinine and urea nitrogen. 1) Mean systolic and diastolic blood pressure of elementary school children were 106.8/67.6mmHg in males and 108.7/69.5mmHg in females. Seven children(4%) of total subjects were found to be hypertensive. Their mean blood pressure was 130.0/86.4mmHg. 2) Urinary excretions of creatinine and urea nitrogen during twenty-four hours were 621.1mg and 1524mg, respectively. The mean daily urinary sodium excretion was 2940mg, which is equivalent to 7.37g NaCl. 3) The daily urinary excretions of other minerals were as follows; potassium was 1301mg, chloride, 4991mg, calcium, 55.7mg and phosphorus, 700.03mg. 4) Salt preference of hypertensive children90.473$\pm$0.275) was significantly higher than those of normotensive children(0.473$\pm$0.213%), but salt threshold was lower in hypertensives(0.105$\pm$0.04%) than normotensives(0.081$\pm$0.022%) (p<0.05). INdices for assessing obesity, such as body weight, triceps skinfold, weight for heigth and body surface area, were high in hypertensive children than normotensive children(p<0.05). But there was no significant difference in energy and nutrient intakes between two groups. 5) Various anthropometric measurements had positive correlations with blood pressure, but body muscle mass(%) had a negative correlation with blood pressure. And daily energy and nutrient intakes were not related to blood pressure. 6) Blood pressures-both systolic and diastolic-were significantly correlated with urinary excretions of potassium and chloride. Daily sodium excretion was related to systolic blood pressure, but no related to diastolci blood pressure. There was no relationship of salt threshold to salt preference, and neither threshold nor preference was related to blood pressure. Results of this study suggest that nutrition education program for children including the information about desirable food habits for the prevention of hypertension should be developed.

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The food Choices and Dietary Preferences of College Women (여대생(女大生)의 음식(飮食) 선택(選擇) 및 기호(嗜好) 성향(性向))

  • Kim, Young-Ja
    • Journal of Nutrition and Health
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    • v.9 no.3
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    • pp.35-45
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    • 1976
  • To investigate the food choices and Dietary Preferences of College Women the number of dishes sold and the sales of the midmeal at the Student Cafeteria, Ewha Womans University were recorded for the fiscal year of 1974; and the observations were made on two consecutive days (June 3rd and 4th, 1974)on what a person has chosen which and how many dishes. The relationship between the number of the consecutive days and the number of foods chosen were treated as Chi square analysis. The results of the food choices and dietary preferences of College Women were as follows: 1. Breads and rice cakes, vegetable dishes, milk and milk products, rice dishes, soups, meat, fish, poultry and eggs, Kimchis and one dish meal were chosen highly; and beverages, stews, and soybeans and soybean products were chosen negligibly. 2. One dish meals, vegetable dishes, meat, fish, poultry and eggs, milk and milk products showed the highest sales volume during the year; rice dishes, breads and rice cakes were the second highest in sales amount; fried foods, snacks and Kimchis showed the range of $3{\sim}6%$ of the total sales; and the beverages, stews, soybean and soybean products were the lowest in sales amount. 3. Seasonal differences were noticed on some dishes. Breads add rice cakes, milk and milk products showed the high selections from March to November and the low, during December to February. Stews, Kimchis, beverages were kept low except sudden rise on February. One dish meal and rice dishes showed almost same level during the year but on February the slight increase were noticed; even level were kept on meat group except the increase to $13{\sim}16%$ on July and December. Fried foods were kept on similar level during the year except the increase on June. Snacks and fruits showed low selections on March, April, November and December and higher at summer. Vegetables, soups, soybean and soybean products showed no different fluctuations in selections during the year. 4. Rolls, salads, milk, hamburgers, fried vegetables, rice with mired vegetables(Bi-bim-bab), and Nang-myun were the single dishes chosen most frequently. 5. There is significant relationship at 0.05 level between the consecutive days and the number of foods chosen. It is concluded that about 50% of the population has taken one item of dishes. The popular combinations were one dish meal and soup or Kimchi; noodles and salads or fried vegetables. It is suggested to set up choice menus with the daily variations, cyclical variations and with the invariable ones; and to recommend one dish meal, breads, partial meals for school lunch; and to study to improve one dish meal, vegetable dishes and fried foods, which are new and inexpensive products. It is also suggested that the feeding of the college students should have to lead the people to the goal that the nutrition education is desired.

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An Energy-Efficient Concurrency Control Method for Mobile Transactions with Skewed Data Access Patterns in Wireless Broadcast Environments (무선 브로드캐스트 환경에서 편향된 엑세스 패턴을 가진 모바일 트랜잭션을 위한 효과적인 동시성 제어 기법)

  • Jung, Sung-Won;Park, Sung-Geun;Choi, Keun-Ha
    • Journal of KIISE:Databases
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    • v.33 no.1
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    • pp.69-85
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    • 2006
  • Broadcast has been often used to disseminate the frequently requested data efficiently to a large volume of mobile clients over a single or multiple channels. Conventional concurrency control protocols for mobile transactions are not suitable for the wireless broadcast environments due to the limited bandwidth of the up-link communication channel. In wireless broadcast environments, the server often broadcast different data items with different frequency to incorporate the data access patterns of mobile transactions. The previously proposed concurrency control protocols for mobile transactions in wireless broadcast environments are focused on the mobile transactions with uniform data access patterns. However, these protocols perform poorly when the data access pattern of update mobile transaction are not uniform but skewed. The update mobile transactions with skewed data access patterns will be frequently aborted and restarted due 4o the update conflict of the same data items with a high access frequency. In this paper, we propose an energy-efficient concurrence control protocol for mobile transactions with skewed data access as well as uniform data access patterns. Our protocol use a random back-off technique to avoid the frequent abort and restart of update mobile transactions. We present in-depth experimental analysis of our method by comparing it with existing concurrency control protocols. Our performance analysis show that it significantly decrease the average response time, the amount of upstream and downstream bandwidth usage over existing protocols.