• Proceedings of the Korean Information Science Society Conference
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• 2005.07a
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• pp.580-582
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• 2005

거리가 상당히 먼 곳에서 고가의 장비를 사용하기 위해서는 사용할 연구 인력이 직접 와야 하는 많은 시간적 비용적 문제가 발생한다. 특히 본원에 장비되어 있는 초고전압 투과전자현미경(High Voltage Electron Microscopy - 이하 HVEM)의 경우 고가의 장비로 지역마다 기기를 구비할 수 없어 사용자는 직접 장비가 있는 연구실까지 와서 사용해야 한다. HVEM은 1천만 배율의 성능을 가진 국내 유일은 물론 전 세계적으로도 손꼽히는 고성능의 투과전자현미경으로 NT(Nano Technology), BT(Bio Technology) 연구에 있어서 핵심적인 역할을 하는 청단 연구기기이다. 따라서 본 논문에서는 그리드 컴퓨터 기술을 이용하여 HVEM을 원격제어 하는 시스템을 구축하였다.

• Applied Microscopy
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• v.36 no.spc1
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• pp.1-7
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• 2006

The three-dimensional structure of an inorganic crystal, $SmZn_{0.67}Sb_2$ (space group $P4/nmm,\;a=4.30(3){\AA}\;and\;c= 10.27(1){\AA}$), was refined by electron crystallography utilizing high voltage electron microscopy (HVEM). Effects of instrumental resolution, image quality, beam damage and specimen tilting on the structure refinement have been evaluated. The instrumental resolution and image quality were the most important factors on the final results in the structure refinement, while the beam damage and specimen tilting effects could be experimentally minimized or controlled. The average phase errors $({\Phi}_{res})$ for the [001], [100] and [110] HVEM images of $SmZn_{0.67}Sb_2$ were $10.1^{\circ},\;9.6^{\circ}\;and\;6.8^{\circ}$, respectively. The atomic coordinates of $SmZn_{0.67}Sb_2$ were consistent within $0.0013{\AA}{\sim}0.0088{\AA}$, compared to the X-ray crystallography data for the same sample.

• Proceedings of the Korean Information Science Society Conference
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• 2005.07a
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• pp.583-585
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• 2005

바이오 및 재료 분야 등 NT(Nano Technology), BT(Bio Technology)에 관련된 연구를 보다 더 수월하게 하기 위해 본원에 설치되어 있는 초고전압 투과전자현미경(High Voltage Electron Microscopy - 이하 HVEM)을 이용한다. HVEM을 통해 획득한 이미지의 정보는 매우 방대하여 하나의 시료를 관찰하는데 있어서 수백 메가 이상의 용량을 차지하고, 연구를 수행하는데 있어서 데이터를 여러 형태로 관찰 분석하기 때문에 수월한 지원을 위해 체계적으로 관리할 수 있는 데이터베이스 시스템이 필요하다. 그러나 일반적인 범용 데이터베이스로는 이러한 대규모의 데이터를 저장할 수 없다. 따라서 본 논문에서는 이러한 용량 데이터를 체계적으로 관리할 수 있도록, 데이터 그리드와 연구 데이터의 정보를 갖는 metadata 테이블을 통해 서로 먼 거리에 있는 연구원들이 데이터를 접근하고 대규모 저장 공간을 갖는 데이터베이스 시스템을 제안한다.

• Applied Microscopy
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• v.42 no.4
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• pp.179-185
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• 2012

Alzheimer's disease (AD) is a progressive neurodegenerative disorder. Neuropathological hallmarks of AD are amyloid plaques, dystrophic neurite, and alteration of subcellular organelles. However, the morpho-functional study of this degenerative process and ultimate neuronal death remains poorly elucidated. In this study, immunohistochemical and ultrastructural analyses were performed to clarify the abnormal morphological alterations caused by the progression of AD in APP/PSEN1 transgenic mice, express human amyloid precursor protein, as a model for AD. In transgenic AD mice brain, the accumulation of Amyloid ${\beta}$ plaques and well-developed dystrophic neurites containing anti-LC3 antibody-positive autophagosomes were detected in the hippocampus and cortex regions. We also found severe disruption of mitochondrial cristae using high-voltage electron microscopy and three-dimensional electron tomography (3D tomography). These results provide morpho-functional evidence on the alteration of subcellular organelles in AD and may help in the investigation of the pathogenesis of AD.

• Applied Microscopy
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• v.31 no.1
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• pp.1-8
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• 2001

The morphological features of neuronal dendritic spines are changed their shapes, sizes and density in response to physiological or pathological conditions . Therefore, exact analysis of spines warrants understanding of neuronal function. The size of the spine is at the borderline of resolution with light microscopy. High voltage electron microscopy Provide excellent resolution of the spines with proper stain techniques thanks to its higher resolution and penetration power. We evaluated more effective staining method for observing dendritic spines after labeling Purkinje cells with anti-calbindin 28 kD immunohistochemistry or Golgi staining methods. 4 fm thickness sections were observed with high voltage electron microscopy and some morphometric analyses were performed. Both Golgi staining and immunohistochemistry revealed the detail structures of the Purkinje cell such as soma, dendrites, and dendritic spines. High voltage electron micrographs with Golgi staining provide more precise morphology and are easy to measure. Average density of spine is $24.5{\pm}3.6/10{\mu}m$ and its length is $1.12{\pm}0.22{\mu}m$. For quantitative analysis of the spines, high voltage electron, micrographs with Golgi staining are more effective. This preliminary result is expected to be useful for further study of spine plasticity in various conditions.

• 한국전자현미경학회:학술대회논문집
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• 2004.11a
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• pp.19-22
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• 2004

• Applied Microscopy
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• v.43 no.3
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• pp.117-121
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• 2013

Mitochondrion is an important intracellular organelle controlling energy production essential for cell survival. In addition, it is closely related to cellular apoptosis and necrosis. Linear, branched, circular, and ball-shaped mitochondria have been reported. Recent research suggests that mitochondrial morphology may reflect functional status of the cell. In this study, we investigated the density and ratio of the each morphological categories of mitochondria in a few normal cultured cells; astrocyte, HeLa and COS7 cells, of which metabolic activities are different, with high voltage electron microscopy. The absolute number and relative number per unit area of mitochondria was largest in astrocyte. But, the proportion of different mitochondrial shape was similar among cells. These results shows the numerical profiles but not morphological profiles of mitochondria are related to the metabolic activity of each cell line.

• Journal of the Korea Convergence Society
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• v.8 no.8
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• pp.197-202
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• 2017

Three-dimensional melanin granules of mummy's hair analyzed using high voltage electron microscopy at 1250kV. The melanin granules in the cortex of mummy's hair located in close proximity to the outer of cortex in the adjacent place of the cuticles. A lot of melanin granule performed in this area. It is a distinguished difference from modern human. While it rotated up to 60 degree counter-clockwise and taken a photo per one degree using high voltage electron microscopy. The melanin granule displayed with long elliptical and variable at the size. The size of granule is measured from $0.3{\sim}0.6{\mu}m$ in minimum diameter and $0.5{\sim}1{\mu}m$ in maximum diameter. Conclusionally, high voltage electron microscope has higher resolution and penetration power than conventional transmission electron microscope that could be load biological thick specimen.

• 한국전자현미경학회:학술대회논문집
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• 2005.05a
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• pp.26-26
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• 2005

• 한국전자현미경학회:학술대회논문집
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• 2002.11a
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• pp.13-14
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• 2002