• Title/Summary/Keyword: High-temperature requirement A2

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A Study on Improvement of the low temperature flex resistance test method about high waterproof materials (고기능성 투습방수 소재의 저온굴곡 시험방법 개선 연구)

  • Lee, Minhee;Moon, Sunjeong;Ko, Hyeji;Hong, Seongdon
    • Journal of Korean Society for Quality Management
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    • v.46 no.3
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    • pp.425-440
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    • 2018
  • Purpose: This study is aimed at developing of the flex resistance testing process at low temperature with the waterproof fabric to suit the military environment, and is designed to fit for the purpose of the waterproof materials in order to optimize the test method by finding out matters to improve from existing the test method and through previous studies. Methods: The test method, which has been applied to flex resistance of existing water-repellent materials, was improved and consequently, differentiated test results could be obtained according to the test temperature, sample size, and flexing method. Results: The testing of the total of 8 samples revealed that performance of the military requirement could hardly be met just by presenting the materials or 2~3 layers when the quality criteria for high functional water repellent fabrics were applied. PTFE(Polytetrafluoroethylene) is preferred to PU(Polyurethane) to be used in the extremely low-temperature environment, but durability under the low-temperature environment may be varied depending on film thickness or laminating technique even if the materials of waterproof films are identical. Therefore, in addition to the material or texture, the test method capable of reflecting durability under the low-temperature environment shall be suggested, and the newly designed test method proposed in this study was shown to suggest differentiated quality criteria by the material. Conclusion: The water resistance measurement and the test method following flex resistance with expanded range of flex will enable the differentiable test of the samples according to the number of repetition. This study is meaningful in that it suggests a differentiable test method capable of establishing a basis of deciding suitable material when selecting military goods made of water repellent material by properly improving the test method.

Improved recovery of active GST-fusion proteins from insoluble aggregates: solubilization and purification conditions using PKM2 and HtrA2 as model proteins

  • Park, Dae-Wook;Kim, Sang-Soo;Nam, Min-Kyung;Kim, Goo-Young;Kim, Jung-Ho;Rhim, Hyang-Shuk
    • BMB Reports
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    • v.44 no.4
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    • pp.279-284
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    • 2011
  • The glutathione S-transferase (GST) system is useful for increasing protein solubility and purifying soluble GST fusion proteins. However, purifying half of the GST fusion proteins is still difficult, because they are virtually insoluble under non-denaturing conditions. To optimize a simple and rapid purification condition for GST-pyruvate kinase muscle 2 (GST-PKM2) protein, we used 1% sarkosyl for lysis and a 1 : 200 ratio of sarkosyl to Triton X-100 (S-T) for purification. We purified the GST-PKM2 protein with a high yield, approximately 5 mg/L culture, which was 33 times higher than that prepared using a conventional method. Notably, the GST-high-temperature requirement A2 (GST-HtrA2) protein, used as a model protein for functional activity, fully maintained its proteolytic activity, even when purified under our S-T condition. This method may be useful to apply to other biologically important proteins that become highly insoluble in the prokaryotic expression system.

The Fermentation Characteristics of Newly Selected Thermotolerant Yeasts at High Temperature

  • Sohn, Ho-Yong;Park, Wan;Jin, Ing-Nyol;Seu, Jung-Hwn
    • Journal of Microbiology and Biotechnology
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    • v.4 no.3
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    • pp.222-229
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    • 1994
  • In order to develop a method of economical production and to reduce energy-consumption in fuel alcohol production, we investigated the fermentation characters of two newly selected thermotolerant yeasts. The RA-74-2 showed stable and superior fermentability between 30 and $40^{\circ}C$ in 20% glucose media in comparison to the industrial strains. The optimum concentration of glucose for economical fermentation at $40^{\circ}C$ was 15-18%, and organic nitrogen was necessary for a satisfactory fermentation. The optimum pH was 4.0 and aeration was adversed for high temperature fermentation. Agitation was an important factor at $40^{\circ}C$ and the addition of magnesium ion 0.2% was required in this experiment. When the inoculum was increased, ethanol productivity as well as the speed of fermentation increased. On the other hand RA-912, which can grow at $48^{\circ}C$, showed similar fermentability between 30-$45^{\circ}C$ in 20% glucose media As the concentration of substrate decreased, fermentation ratio increased at $45^{\circ}C$ (45%, 65%, 95% fermentation ratio in 20%, 15%, 10% glucose media, respectively). Also, requirement of organic nitrogen and magnesium ion in RA-912 was similar in RA-74-2. The optimum pH for fermentation was 5.0, and the effects of agitation were enhanced at $37^{\circ}C$ than at $45^{\circ}C$. As the inoculum was increased, fermentation speed became more enhanced but the ethanol productivity was less affected. RA-912 showed fermentability with various substrates. Among the substrates used, inulin was the most promising substrate for the high-temperature fermentation. When 14.5% inulin was used as the substrate, 93% and 55% fermentation ratios were shown at $37^{\circ}C$ and $45^{\circ}C$, respectively.

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Preliminary Result of Uncertainty on Variation of Flowering Date of Kiwifruit: Case Study of Kiwifruit Growing Area of Jeonlanam-do (기후변화에 따른 국내 키위 품종 '해금'의 개화시기 변동과 전망에 대한 불확실성: 전남 키위 주산지역을 중심으로)

  • Kim, Kwang-Hyung;Jeong, Yeo Min;Cho, Youn-Sup;Chung, Uran
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.1
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    • pp.42-54
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    • 2016
  • It is highly anticipated that warming temperature resulting from global climate change will affect the phenological pattern of kiwifruit, which has been commercially grown in Korea since the early 1980s. Here, we present the potential impacts of climate change on the variations of flowering day of a gold kiwifruit cultivar, Haegeum, in the Jeonnam Province, Korea. By running six global climate models (GCM), the results from this study emphasize the uncertainty in climate change scenarios. To predict the flowering day of kiwifruit, we obtained three parameters of the 'Chill-day' model for the simulation of Haegeum: $6.3^{\circ}C$ for the base temperature (Tb), 102.5 for chill requirement (Rc), and 575 for heat requirement (Rh). Two separate validations of the resulting 'Chill-day' model were conducted. First, direct comparisons were made between the observed flowering days collected from 25 kiwifruit orchards for two years (2014-15) and the simulated flowering days from the 'Chill-day' model using weather data from four weather stations near the 25 orchards. The estimation error between the observed and simulated flowering days was 5.2 days. Second, the model was simulated using temperature data extracted, for the 25 orchards, from a high-resolution digital temperature map, resulting in the error of 3.4 days. Using the RCP 4.5 and 8.5 climate change scenarios from six GCMs for the period of 2021-40, the future flowering days were simulated with the 'Chill-day' model. The predicted flowering days of Haegeum in Jeonnam were advanced more than 10 days compared to the present ones from multi-model ensemble, while some individual models resulted in quite different magnitudes of impacts, indicating the multi-model ensemble accounts for uncertainty better than individual climate models. In addition, the current flowering period of Haegeum in Jeonnam Province was predicted to expand northward, reaching over Jeonbuk and Chungnam Provinces. This preliminary result will provide a basis for the local impact assessment of climate change as more phenology models are developed for other fruit trees.

Design and Development of 600 W Proton Exchange Membrane Fuel Cell (600 W급 연료전지(PEMFC)의 설계 및 제작)

  • Kim, Joo-Gon;Chung, Hyun-Youl;Bates, Alex;Thomas, Sobi;Son, Byung-Rak;Park, Sam;Lee, Dong-Ha
    • Journal of the Korean Solar Energy Society
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    • v.34 no.4
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    • pp.17-22
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    • 2014
  • The design of a fuel cells stack is important to get optimal output power. This study focuses on the evaluation of fuel cell system for unmaned aerial vehicles (UAVs). Low temperature proton exchange membrane (LTPEM) fuel cells are the most promising energy source for the robot applications because of their unique advantages such as high energy density, cold startup, and quick response during operation. In this paper, a 600 W open cathode LTPEM fuel cell was tested to evaluate the performance and to determine optimal operating conditions. The open cathode design reduces the overall size of the system to meet the requirement for robotic application. The cruise power requirement of 600 W was supported entirely by the fuel cell while the additional power requirements during takeoff was extended using a battery. A peak of power of 900 W is possible for 10 mins with a lithium polymer (LiPo) battery. The system was evaluated under various load cycles as well as start-stop cycles. The system response from no load to full load meets the robot platform requirement. The total weigh of the stack was 2 kg, while the overall system, including the fuel processing system and battery, was 4 kg.

Analyses on Thermal Stability and Structural Integrity of the Improved Disposal Systems for Spent Nuclear Fuels in Korea

  • Lee, Jongyoul;Kim, Hyeona;Kim, Inyoung;Choi, Heuijoo;Cho, Dongkeun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.18 no.spc
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    • pp.21-36
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    • 2020
  • With respect to spent nuclear fuels, disposal containers and bentonite buffer blocks in deep geological disposal systems are the primary engineered barrier elements that are required to isolate radioactive toxicity for a long period of time and delay the leakage of radio nuclides such that they do not affect human and natural environments. Therefore, the thermal stability of the bentonite buffer and structural integrity of the disposal container are essential factors for maintaining the safety of a deep geological disposal system. The most important requirement in the design of such a system involves ensuring that the temperature of the buffer does not exceed 100℃ because of the decay heat emitted from high-level wastes loaded in the disposal container. In addition, the disposal containers should maintain structural integrity under loads, such as hydraulic pressure, at an underground depth of 500 m and swelling pressure of the bentonite buffer. In this study, we analyzed the thermal stability and structural integrity in a deep geological disposal environment of the improved deep geological disposal systems for domestic light-water and heavy-water reactor types of spent nuclear fuels, which were considered to be subject to direct disposal. The results of the thermal stability and structural integrity assessments indicated that the improved disposal systems for each type of spent nuclear fuel satisfied the temperature limit requirement (< 100℃) of the disposal system, and the disposal containers were observed to maintain their integrity with a safety ratio of 2.0 or higher in the environment of deep disposal.

Determination of Energy and Time Requirement for Cooking Pigeon Pea (Cajanus cajan)

  • Akinoso, Rahman;Oladeji, Ojeronke Dewum
    • Journal of Biosystems Engineering
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    • v.42 no.1
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    • pp.56-61
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    • 2017
  • Purpose: High energy requirement and long cooking time are limiting consumption of pigeon pea (Cajanus cajan), a nutritious food. This study was performed to estimate energy and time demand by different methods of cooking pigeon pea. Methods: Pigeon pea (150 g) was soaked in 2.0 L of water at ambient temperature ($29{\pm} 2^{\circ}C$) to determine hydration behavior. Cooking experiments were conducted using aluminum and pressure-cooking pots. Efficiency of cooking was evaluated using four types of cooking appliances (kerosene, liquefied petroleum gas (LPG), electric, and charcoal stoves). Normal (continuous heating until the food was satisfactorily cooked) and control (controlling the energy input to closely match the actual energy required) cooking were conducted. Energy requirement and duration of cooking were determined using standard procedures. Results: Soaking increased moisture content from 11.99 to 30.01% in 90 min, while water absorption rate decreased with soaking duration. In cooking 150 g of pigeon pea using kerosene stove, presoaked normal pressure-pot cooking method consumed the least energy (10 800 kJ) and time (205 min), while unsoaked normal cooking consumed the highest energy (18 450 kJ) and time (336 min). Using LPG stove, unsoaked normal cooking method required the highest energy (52 470 kJ), while presoaked control pressure-pot required the least energy (14 405 kJ). For electric stove, the lowest energy (15 560 kJ) and shortest duration (105 min) were recorded during control cooking of presoaked sample in the pressure-pot. Conclusions: Control cooking was not practicable using charcoal stove. Generally, kerosene stove consumed the least energy, while electric stove was found to have the shortest duration of cooking.

Relationship Between Heat Unit Requirement and Growth and Yield of Mulberry, Morns indica L.

  • Sarkar A.;Rekha M.;Keshavacharyulu K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.1
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    • pp.65-68
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    • 2005
  • Growth and development of a plant over a growing season is closely related to the daily accumulation of heat. Heat unit measured by accumulated growing degree days (GDD) is becoming increasingly popular to estimate the growth of a plant or even in insect. GDD or heat accumulation per day is measured by calculating average daily temperature and then subtracting the base temperature below which growth does not occur. Heat accumulation per day is added for the desired period and accumulated GDD is determined. The present study was conducted in five seasons in an established garden with K-2, S-36 and V-1 mulberry varieties belonging to Morus indica L. grown under completely irrigated condition at the farm of CSRTI, Mysore during 2001 - 2002. Plants were pruned in each season and the growth of the plant measured by total shoot length and fresh leaf yield was recorded at an interval of 5 days starting from 30 days of pruning (DAP) to 70 days when all the plants were pruned. The accumulated GDD for the corresponding days were recorded and used for analysis. Accumulated growing degree days (GDD) have been found to be perfectly correlated with both growth and yield in all the seasons in all the varieties studied. The high $R^2$ values indicated a strong relationship between the accumulated GDD and, growth and yield of mulberry.

Kraft Pulping of Sapwood-A Sawmill Waste

  • Jahant M. Sarwar;Chowdhury D.A. Nasima;Islam M. Khalidul;Mun Sung Phil
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.37 no.5 s.113
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    • pp.41-49
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    • 2005
  • This paper deals the effect of anthraquinone (AQ) on the contribution of sulphidity in kraft pulping of sapwood. The pulping conditions namely- active alkali concentration, pulpingtime, temperature and liquor ratio were varied in low ($15\%$) and high ($30\%$) sulphidity. $0.1\%$ AQ was added in the low and high sulphidity pulping with varying active alkali concentration and cooking time. At optimum conditions, low sulphidity kraft process produced about $44\%$ pulp yield with kappa number of about 23. But in high sulphidity kraft process kappa number was reduced to about 20 at the same yield. An addition of AQ reduced alkali requirement by $2\%$ on oven dried raw material and cooking time by 1 hour to produce pulp yield of about $44\%$ at kappa number 20. AQ is more effective in low sulphidity pulping than the high sulphidity pulping. The breaking length of kraft-AQ pulp was slightly higher than that of kraft pulp.

Thermal Behavior and Leaf Temperature in a High Pressure Sodium Lamp Supplemented Greenhouse (고압나트륨등 보광 온실의 열적 거동 및 엽온 분석)

  • Seungri Yoon;Jin Hyun Kim;Minju Shin;Dongpil Kim;Ji Wong Bang;Ho Jeong Jeong;Tae In Ahn
    • Journal of Bio-Environment Control
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    • v.32 no.1
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    • pp.48-56
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    • 2023
  • High-pressure sodium (HPS) lamps have been widely used as a useful supplemental light source to emit sufficient photosynthetically active radiation and provide a radiant heat, which contribute the heat requirement in greenhouses. The objective of this study to analyze the thermal characteristics of HPS lamp and thermal behavior in supplemented greenhouse, and evaluate the performance of a horizontal leaf temperature of sweet pepper plants using computational fluid dynamics (CFD) simulation. We simulated horizontal leaf temperature on upper canopy according to three growth stage scenarios, which represented 1.0, 1.6, and 2.2 plant height, respectively. We also measured vertical leaf and air temperature accompanied by heat generation of HPS lamps. There was large leaf to air temperature differential due to non-uniformity in temperature. In our numerical calculation, thermal energy of HPS lamps contributed of 50.1% the total heat requirement on Dec. 2022. The CFD model was validated by comparing measured and simulated data at the same operating condition. Mean absolute error and root mean square error were below 0.5, which means the CFD simulation values were highly accurate. Our result about vertical leaf and air temperature can be used in decision making for efficient thermal energy management and crop growth.