• Title/Summary/Keyword: High magnification

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Applications of the Scanning Electron Microscope (주사형(走査型) 전자현미경(電子顯微鏡)의 응용분야(應用分野))

  • Kim, Yong-Nak
    • Applied Microscopy
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    • v.2 no.1
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    • pp.39-46
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    • 1972
  • There are many kinds of microscopes suitable for general studies; optical microscopes(OM), conventional transmission electron microscopes (TEM), and scanning electron microscopes(SEM). The optical microscopes and the conventional transmission electron microscopes are very familiar. The images of these microscopes are directly formed on an image plane with one or more image forming lenses. On the other hand, the image of the scanning electron microscope is formed on a fluorescent screen of a cathode ray tube using a scanning system similar to television technique. In this paper, the features and some applications of the scanning electron microscope will be discussed briefly. The recently available scanning electron microscope, combining a resolution of about $200{\AA}$ with great depth of field, is favorable when compared to the replica technique. It avoids the problem of specimen damage and the introduction of artifacts. In addition, it permits the examination of many samples that can not be replicated, and provides a broader range of information. The scanning electron microscope has found application in diverse fields of study including biology, chemistry, materials science, semiconductor technology, and many others. In scanning electron microscopy, the secondary electron method. the backscattererd electron method, and the electromotive force method are most widely used, and the transmitted electron method will become more useful. Change-over of magnification can be easily done by controlling the scanning width of the electron probe. It is possible. to continuously vary the magnification over the range from 100 times to 1.00,000 times without readjustment of focusing. Conclusion: With the development of a scanning. electron microscope, it is now possible to observe almost all-information produced through interactions between substances and electrons in the form of image. When the probe is properly focused on the specimen, changing magnification of specimen orientation does not require any change in focus. This is quite different from the conventional transmission electron microscope. It is worthwhile to note that the typical probe currents of $10^{-10}$ to $10^{-12}\;{\AA}$ are for below the $10^{-5}$ to $10^{-7}\;{\AA}$ of a conventional. transmission microscope. This reduces specimen contamination and specimen damage due to heatings. Outstanding features of the scanning electron microscope include the 'stereoscopic observation of a bulky or fiber specimen in high resolution' and 'observation of potential distribution and electromotive force in semiconductor devices'.

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Preparation of Cellulose Nanofibril/Regenerated Silk Fibroin Composite Fibers

  • Lee, Ji Hye;Bae, Chang Hyun;Park, Byung-Dae;Um, In Chul
    • International Journal of Industrial Entomology and Biomaterials
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    • v.26 no.2
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    • pp.81-88
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    • 2013
  • Wet-spun silk fibers have attracted the attention of many researchers because of 1) the unique properties of silk as a biomaterial, including good biocompatibility and cyto-compatability and 2) the various methods available to control the structure and properties of the fiber. Cellulose nanofibrils (CNFs) have typically been used as a reinforcing material for natural and synthetic polymers. In this study, CNF-embedded silk fibroin (SF) nanocomposite fibers were prepared for the first time. The effects of CNF content on the rheology of the dope solution and the characteristics of wet-spun CNF/SF composite fibers were also examined. A 5% SF formic acid solution that contained no CNFs showed nearly Newtonian fluid behavior, with slight shear thinning. However, after the addition of 1% CNFs, the viscosity of the dope solution increased significantly, and apparent shear thinning was observed. The maximum draw ratio of the CNF/SF composite fibers decreased as the CNF content increased. Interestingly, the crystallinity index for the silk in the CNF/SF fibers was sequentially reduced as the CNF content was increased. This phenomenon may be due to the fact that the CNFs prevent ${\beta}$-sheet crystallization of the SF by elimination of formic acid from the dope solution during the coagulation process. The CNF/SF composite fibers displayed a relatively smooth surface with stripes, at low magnification (${\times}500$). However, a rugged nanoscale surface was observed at high magnification (${\times}10,000$), and the surface roughness increased with the CNF content.

AN EXPERIMENTAL STUDY ON THE DIMENSIONAL CHANGES OF RELINED DENTURES AND MECHANICAL PROPERTIES AND SURFACE TEXTURES OF SEVERAL RESINS USED IN DENTURE RELINING (수종 레진으로 의치상 조직면 개조시 의치상의 크기변화와 물리적 성질 및 표면상태 비교 연구)

  • Lee, Chang-Han;Kim, Yung-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.28 no.1
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    • pp.25-41
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    • 1990
  • The purpose of this study was to evaluate and compare the dimensional changes of relined dentures with a light-curing resin, a heat-curing resin, and a direct, hard reline resin. And also to measure the transverse strength, impact strength, surface hardness of the three resins used in relining. The surface textures of three resins also of evaluated by using scanning electron microscope. Through analyses on the data from this study, the following conclusions were obtained. 1. Impact strength of heat-curing resin was highest, and direct, hard reline resin higher, light-curing resin lowest. 2. Transverse strength of heat-curing resin was highest, and direct, hard reline resin and light-curing resin was lower and not signiicantly different. 3. Surface hardness of light-curing resin was lighest, heat-curing resin higher, and direct, hard reline resin was lowest. 4. After storage of the relined dentures for 1 day and 1 week in water at room temperature, linear shrinkage of distance between the reference points in the maxillary base relined with direct, hard reline resin was lowest, and those relined with light-curing resin and heat-curing resin were lower and were not significantly different. 5. After storage for 4 weeks in orator at room tempeature, linear shrinkage of distance between ridge crests of dentures relined with heat-curing resin was highest and that of distance between denture borders was not significantly different. 6. The dimensional changes of relined dentures during storage in water was not significant except those of distance between denture borders relined with light-curing resin at 1 day and 1 week storage in water. 7. At low magnification (x40) of SEM examination, the surface textures of three resins were similar except light-curing resin which had some defects. At high magnification (x200), the surface textures of hard, direct reline resin were smooth with little defects, but those of heat-curing resin and light-curing resin w ere irregular.

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Effects of Caponization on Bone Characteristics and Histological Structure in Chickens

  • Chen, Kuo-Lung;Chang, Ming-Huang;Tsay, Shiow-Min;Hurng, Huaang-Youh;Chiou, Peter Wen-Shyg
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.2
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    • pp.245-251
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    • 2006
  • The aim of this study was to investigate the effects of caponization on the bone characteristics, biomechanical property and histology in Taiwan country chickens fed to market age of 26 wks. Male Taiwan country chickens $D{\times}L_2$ were caponized or sham-operated at 8 wks of age, and selected healthy sham-operated and completely caponized chickens (prominent degenerated comb) were selected at 16 wks old and fed to 26 wks old for the trials. Fifteen intact male chickens (Intact), sham-operated chickens (Sham) and caponized chickens (Capon) were assigned for trial 1, and sixteen Intact and Capon were assigned for trial 2. Results in trial 1 showed that the abdominal fat and relative abdominal fat weights of Capon were significantly heavier than Intact and Sham (p<0.05), while the tibia weight and relative weight were the lightest (p<0.05). The tibia breaking strength, bending moment and stress of Capon were the poorest among groups (p<0.05). The trial 2 produced the similar observation that Capon were significantly lighter than Intact (p<0.05) in the tibia weight, relative tibia weight and their biomechanical properties. On histological determinations, Capon showed a thinner cartilage end and fewer chondrocytes (about 50%) and trabecular, and bigger marrow cavity; while decreased hemopoietic cells number with increased adipocytes than Intact observed by H&E stain and at low magnification. At high magnification, Capon showed a decrease in the chondrocyte size by 33 to 50%, with smaller nucleus located near the cell membrane, and exhibited monocellular form chondrocytes. Capon also showed a less strongly acidic sulfated mucosubstance with weaker dyeing property within cartilage zone, and smaller chondrocytes size by Alcian blue stain.

Estimation of Dynamic Response of Advanced Composite Material Decks for Bridges Application under Various Vehicle Driving Velocities (복합재료 교량 바닥판의 주행속도에 따른 동적응답 평가)

  • 천경식;장석윤
    • Composites Research
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    • v.16 no.6
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    • pp.23-32
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    • 2003
  • Applications of advanced composite material in construction field are tending upwards and development of all composite material bridges is making progress rapidly in home and abroad due to their high strength to weight ratio. This paper formulated the dynamic responses of the laminated composite structures subjected to moving load and analyzed the various dynamic behaviors using the finite element method. The nondimensionalized natural frequencies of a simply supported square-laminated composite plate are considered for verifications. Mode superposition and Newmark direct integration method are applied for moving load analysis. For structural models, dynamic magnification factor calculated for various velocities of the moving load and displacements characteristics of laminated composite structures due to the moving load are investigated theoretically Numerical results are presented to study the effects of lamination scheme, stacking sequence, and fiber angle for laminated composite structures during moving load. The various results on moving load and lamination through numerical analysis will present an important basic data for development and grasp the behavior of all composite material bridges.

Methylene blue chromoendoscopy is more useful in detection of intestinal metaplasia in the stomach than mucosal pit pattern or vessel evaluation and predicts advanced Operative Link on Gastric Intestinal Metaplasia stages

  • Justyna Wasielica-Berger;Pawel Rogalski;Anna Pryczynicz;Agnieszka Swidnicka-Siergiejko;Andrzej Dabrowski
    • Clinical Endoscopy
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    • v.56 no.2
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    • pp.203-213
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    • 2023
  • Background/Aims: Intestinal metaplasia (IM) of the stomach is a precancerous condition that is often not visible during conventional endoscopy. Hence, we evaluated the utility of magnification endoscopy and methylene blue (MB) chromoendoscopy to detect IM. Methods: We estimated the percentage of gastric mucosa surface staining with MB, mucosal pit pattern, and vessel visibility and correlated it with the presence of IM and the percentage of metaplastic cells in histology, similar to the Operative Link on Gastric Intestinal Metaplasia (OLGIM) stage. Results: IM was found in 25 of 33 (75.8%) patients and in 61 of 135 biopsies (45.2%). IM correlated with positive MB staining (p<0.001) and other than dot pit patterns (p=0.015). MB staining indicated IM with better accuracy than the pit pattern or vessel evaluation (71.7% vs. 60.5% and 49.6%, respectively). At a cut-off point of 16.5% for the MB-stained gastric surface, the sensitivity, specificity, and accuracy of chromoendoscopy in the detection of advanced OLGIM stages were 88.9%, 91.7%, and 90.9%, respectively. The percentage of metaplastic cells detected on histology was the strongest predictor of positive MB staining. Conclusions: MB chromoendoscopy can serve as a screening method for detecting advanced OLGIM stages. MB mainly stains IM areas with a high concentration of metaplastic cells.

High performance active tuned mass damper inerter for structures under the ground acceleration

  • Li, Chunxiang;Cao, Liyuan
    • Earthquakes and Structures
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    • v.16 no.2
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    • pp.149-163
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    • 2019
  • By integrating an active tuned mass damper (ATMD) and an inerter, the ATMDI has been proposed to attenuate undesirable oscillations of structures under the ground acceleration. Employing the mode generalized system, the dynamic magnification factors (DMF) of the structure-ATMDI system are formulated. The criterion can then be defined as the minimization of maximum values of the DMF of the controlled structure for optimum searching. By resorting to the defined criterion and the particle swarm optimization (PSO), the effects of varying the crucial parameters on the performance of ATMDI have been scrutinized in order to probe into its superiority. Furthermore, the results of both ATMD and tuned mass dampers inerter (TMDI) are included into consideration for comparing. Results corroborate that the ATMDI outperforms both ATMD and TMDI in terms of the effectiveness and robustness. Especially, the ATMDI may greatly reduce the demand on both the mass ratio and inerter mass ratio, thus being capable of further miniaturizing both the ATMD and TMDI. Likewise the miniaturized ATMDI still keeps nearly the same stroke as the TMDI with a larger mass ratio. Hence, the ATMDI is deemed to be a high performance control device with the miniaturization and suitable for super-tall buildings.

Wafer Packing Box for Vibration Suppression Material Optimization (진동 억제를 위한 Wafer Packing Box 재료 최적화)

  • Yoon, Jae-Hoon;Hur, Jang-Wook;Yi, Il-Hwan
    • Journal of the Semiconductor & Display Technology
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    • v.21 no.2
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    • pp.51-56
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    • 2022
  • Recently, the demand for semiconductors is expanded to various industries, and the use of high-quality and high-performance chips is increasing. With the trend, the diameter magnification and high integration of the semiconductor wafers are mandatory. As a result, there is a growing demand for the productivity improvement and the surface precision. There have been many studies on the stabilization of the wafer manufacturing processes in order to satisfy those specifications. Many complaints have been appealed by the wafer buyers that there are many unacceptable wafers with surface defects and foreign material adhesion which are caused by the vibrations during transportation. This study intends to derive the material improvement of the packing box of the wafers to suppress the vibrations of the box, and eventually to reduce the surface defects and the foreign material adhesion. The result shows that optimal material can substantially decrease the vibration of the packing box.

Rotational Prism Stitching Interferometer for High-resolution Surface Testing (고해상도 표면 측정을 위한 회전 프리즘 정합 간섭계)

  • In-Ung Song;Woo-Sung Kwon;Hagyong Khim;Yun-Woo Lee;Jong Ung Lee;Ho-Soon Yang
    • Korean Journal of Optics and Photonics
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    • v.34 no.3
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    • pp.117-123
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    • 2023
  • The size of an optical surface can significantly affect the performance of an optical system, and high spatial frequency errors have a greater impact. Therefore, it is crucial to measure the surface figure error with high frequency. To address this, a new method called rotational prism stitching interferometer (RPSI) is proposed in this study. The RPSI is a type of stitching interferometer that enhances spatial resolution, but it differs from conventional stitching interferometers in that it does not require the movement of either the mirror tested or the interferometer itself to obtain sub-aperture interferograms. Instead, the RPSI uses a beam expander and a rotating Dove prism to select particular sub-apertures from the entire aperture. These sub-apertures are then stitched together to obtain a full-aperture result proportional to the square of the beam expander's magnification. The RPSI's effectiveness was demonstrated by measuring a 40 mm diameter spherical mirror using a three-magnification beam expander and comparing the results with those obtained from a commercial interferometer. The RPSI achieved surface testing results with nine times higher sampling density than the interferometer alone, with a small difference of approximately 1 nm RMS.

Evaluation of Validity Thyroid Scintigraphy Using Parallel Hole Collimator (갑상샘 신티그래피 검사 시 평행다공형 조준기 적용의 유효성 평가)

  • Su-Young Park;Ji-Youn Kim;Sung-Min Ahn
    • Journal of the Korean Society of Radiology
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    • v.18 no.1
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    • pp.27-36
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    • 2024
  • In this study, When acquisition thyroid scintigraphy images, a parallel hole collimator was applied, and the difference from the pinhole collimator was quantitatively analyzed under each image acquisition condition. Visual size, resolution, sensitivity, signal to noise ratio (SNR), and contrast to noise ratio (CNR) were evaluated using thyroid phantom and point source. When comparing visual size, it was confirmed that an image similar to the size of the pinhole collimator could be obtained only when a magnification ratio of about 2.00 to 2.09 times when applying a parallel hole collimator. There was no tendency in FWHM(mm) measurement using a point source, and sensitivity was high in the parallel hole collimator. SNR and CNR were high when using a low magnification ratio, matrix size of 128×128, and a parallel hole collimator. In images of similar size to the naked eye, when the matrix size was the same, both SNR and CNR were high in the pinhole collimator. Therefore, when performing a thyroid scintigraphy test, if appropriate conditions are set according to the situation of each hospital and a parallel hole collimator is applied, it can be a good option in terms of equipment utilization and work efficiency.