• Journal of The Korean Society of Grassland and Forage Science
• /
• v.40 no.4
• /
• pp.285-290
• /
• 2020

To understand the role of small heat shock protein (sHSPs) in rice plant response to various stresses such as the heat and oxidative stresses, a cDNA encoding a 24.1 kDa mitochondrial small HSP (Oshsp24.1) was isolated from rice by rapid amplification of cDNA ends (RACE) PCR. The deduced amino acid sequence shows very high similarity with other plant small HSPs. DNA gel blot analysis suggests that the rice genome contains more than one copy of Oshsp24.1. High level of expression of Oshsp24.1 transcript was observed in rice seedlings in response to heat, methyl viologen, hydrogen peroxide, ozone, salt and heavy metal stresses. Recombinant OsHSP24.1 protein was produced in E. coli cells for biochemical assay. The protein formed oligomeric complex when incubated with Sulfo-EGS (ethylene glycol bis (succinimidyl succinate)). Our results shows that Oshsp24.1 has an important role in abiotic stress response and have potential for developing stress-tolerant plants.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.19 no.3
• /
• pp.51-56
• /
• 2020

Research into natural refrigerants that use CO₂, instead of chlorofluorocarbons and hydrofluorocarbons, has increased due to the environmental problems caused by ozone depletion. CO₂ refrigerants are more environmentally friendly than conventional refrigerants because they have better latent heat of evaporation and heat transfer efficiency properties. However, they have very low critical temperatures and require high design pressures; therefore, pressure control valves, which reduce the pressure of the CO₂ refrigerant to a safe level and apply it to the refrigerant air conditioning system, are necessary to secure stability against high pressure. In the present study, we evaluated the flow characteristics and valve performance of the pressure control valve using a CO₂ refrigerant by measuring the pressure, velocity, and flow coefficient. In addition, we examined the applied forces caused by the internal pressure from the highly pressurized CO₂ refrigerant and required thrust characteristics.

• Journal of Korean Elementary Science Education
• /
• v.28 no.2
• /
• pp.142-153
• /
• 2009

The purpose of this study was to survey primary students' perception of the global and local environmental issues. The subjects were 853 fifth primary students in Seoul, Busan, Daejeon, Anyang, Gimpo and Cheongju. The results were as follows: First, the perception level of the global environmental issues were global warming (M=3.99), drinking-water pollution (M=3.92), acid rain (M=3.77), yellow dust (M=3.66), ozone depletion (M=3.57), deforestation in tropical areas (M=3.52), desertification (M=3.36), biodiversity (M=3.40) and their perception level of local environmental issues were waste disposal (M=3.87), air pollution (M=3.74), bad smell (M=3.70), noise (M=3.61), river pollution (M=3.57), soil pollution (M=3.44). Second, it was shown that the metropolitan students' perception levels of environmental issues were higher than the non-metropolitan students'. There wasn't a significant difference in the perception levels of two scale environmental issues and characteristics for sex (p>.05). Third, the result of analyzing on the effects between characteristics was that the factors like 'environmental interest' and 'impact on personal life' had a strong influence on the 'behavior will' to solve the global environmental issues, and 'environmental interest' and 'personal knowledge' had a strong influence on the 'behavior will' to solve the local environmental issues. Fourth, the result of analyzing on the characteristics according to the types of the environmental issues showed that 'personal knowledge', 'environmental interest' and 'behavior will' were high in the global environmental issues (p<.01) and 'virtual contamination', 'human responsibility' and 'impact on personal life' were high in the local environmental issues (p<.05).

• Journal of Korean Society for Atmospheric Environment
• /
• v.18 no.5
• /
• pp.355-362
• /
• 2002

There has been relatively a few studies that focused on evaluation of uncertainty for standard methods by which criteria pollutants are analyzed in ambient air. Especially, uncertainty evaluation has not been made yet for sampling and analysis of airborne NO$_2$. Ambient NO$_2$ has been thought to be a major criteria pollutant worldwide because of the potential of ozone formation as well as of its own toxicity. In this study, we tried to assess uncertainties associated with the every step of sampling and of analytical procedure of Griess-Saltzman method. Quality assurance (QA) and quality control (QC) were also emphasized with the uncertainty characterization. The use of Griess-Saltzman method for ambient NO$_2$ analysis showed very uniform daily concentration distribution with the mean of 10.8 ppb and the standard deviation of 1.08ppb during the sampling period. However, seven daily samples collected at the same sampling time and place exhibited highly different concentration distribution. Therefore, we evaluated uncertainties associated with sampling and analysis through the precise application of ISO Guide. Estimates of expanded uncertainties for a total of 62 samples fell in a relatively broad range of 5.17% to 11.85%. On the other hand. the expanded uncertainties were smaller for the high concentration range of greater than 15ppb.

• Journal of Korean Society of Water and Wastewater
• /
• v.21 no.4
• /
• pp.395-407
• /
• 2007

Disinfection by-products(DBPs) are formed through the reaction between chlorine and natural organic matter(NOM) in water treatment. For reducing the formation of chlorinated DBPs in the drinking water treatment, there is a need to evaluate the behavior of NOM fractions and the occurrence of DBPs for each fraction. Among the six fractions of NOM, the removal of HPOA and HPIN got accomplished through coagulation and sedimentation processes. Advanced water treatment processes were found to be most significant to remove the HPOA and HPON. It was found that HPOA made the most THMFP level than any other fractions and HPIA and HPOA formed higher HAAFP. The fraction of NOM with MW less than 1k Da was 32.5~54.3% in intake raw water. Mostly the organic matter with MW more than 1k Da was removed through coagulation and sedimentation in the drinking water treatment processes. In case of advanced water treatment processes, the organic matter with MW 1k~100k Da decreased by means of ozone oxidation for high molecular weight substances. As the result low molecular organic matter increased. In the BAC and GAC processes, the organic matter with MW less than 100k Da decreased.

• Plant Resources
• /
• v.8 no.1
• /
• pp.52-59
• /
• 2005

A cDNA, PSOD1, encoding cytosolic copper/zinc superoxide dismutase (CuZn-SOD) was cloned and characterized from a full length cDNA library prepared from Populus alba${\times}$Populus glandulosa cultured in vitro. A PSOD1, is 725 nucleotides long and has an open reading frame of 459 bp with 152 amino acid residues (pI 5.43). The deduced amino acid sequence of PSOD1 perfect matched to the previously reported CuZn-SOD (CAC33845.1). Consensus amino acid residues (His-45, -47, -62, -70, -79, -119) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of PSOD1 exhibited the high level of similarity from 100 to $85\%$ among previously registered SOD genes. The expression of PSOD1 in poplar increased at the 1 mM $H_{2}O_2$ and drought stress during 30 min and 60 min, but the ozone treated poplar increased at 30 min in the early time and then decreased at 60 min.

• Proceedings of the SAREK Conference
• /
• 2006.06a
• /
• pp.1225-1231
• /
• 2006

Recently, as the climate of temperature change has happened worldwide, To solve this problem, Kyoto protocol was taken to regulate global warming on Feb. 2005 and each country is making efforts to prevent global warming. In the automotive industry, R-134a refrigerant is widely used most these days because it has zero ODP(Ozone Depletion Potential). But R-134a GWP(Global Warming Potential) is so high. Therefore, replacement refrigerant desperately is needed as a alternative refrigerant. So, R-l52a is considered as one of the alternative refrigerants due to zero ODP and lower GWP against as required on Europe Committee. In this paper, performance of the air conditioning system between R-134a and R-152a is investigated experimentally. In the bench level, cooling capacity, condensing capacity, COP of automotive air conditioning system are ovaluated by means of air velocity entering the condenser and compressor revolution speed with optimized charge refrigerant quantity. Result of this study, R-152a refrigerant shows the possibility as alternative refrigerant of current R-134a in automotive air conditioning system.

• International Union of Geodesy and Geophysics Korean Journal of Geophysical Research
• /
• v.23 no.1
• /
• pp.18-33
• /
• 1995

Under the synoptic scale strong westerly winds flowing over the large steep mountains in the eastern coastal region, the strong downslope wind storms such as internal gravity waves should be generated in the lee-side of mountain. Int he daytime as sea breeze circulation induced by meso-scale thermal forcing from sea toward inland confines to the offshore side of coastal sites due to the eastward internal gravity waves. Thus, surface winds near the coastal seas were relatively weaker than those in the open sea or the inland sites. Evidently, two different kinds of atmospheric circulations such as an internal gravity wave circulation with westerly wind and a sea breeze circulation with both easterly wind near the sea surface and westerly in the upper level were apparently produced. Under this situation the atmospheric pollutants at Kangnung city should be trapped by two different circulations in the opposite directions and resulted in the high concentrations of Total Suspended Particles (TSP) and ozone (O3). At night a meso-scale land breeze from land toward the more intensification of westerly winds in the coastal regions. The concentrations of TSP controled by the strong surface winds blowing from the mountain side toward the coastal sea were relatively higher at night than those in the daytime case and the concentrations of O3 due to the downward transport of ozone from the upper atmosphere toward the surface were also much higher at night than during the day. Consequently, the atmospheric pollutant concentrations in the mountainous coastal region under the downslope wind storms were higher than those after and before the occurrences of wind storms.

• Journal of the Architectural Institute of Korea Planning & Design
• /
• v.34 no.2
• /
• pp.3-11
• /
• 2018

Korea has achieved a rapid economic development and with the increase in population and national income and the expansion of social and economic activities, energy consumption has rapidly increased too. Energy consumption per head has constantly increased and currently, power consumption per head is 7.5 times bigger than in 1985. Buildings occupy 25% of total energy consumption and especially, 50% of total energy is consumed for heating and cooling. In this situation, multi-family housing, which has constantly been increased, has an energy saving rate of 1.9%, which is the lowest level and this makes the government's energy policy for sustainable energy system development useless. Besides, energy consumption leads to secondary problems, such as air, water and marine pollution and heat pollution and wastewater/drainage and the increased use of fossil fuel is a fundamental reason for ozone layer destruction and global warming. Therefore, efficient energy consumption plans are required. This study aims to analyze energy performance in each block type of high-rise and diversified multi-family housing that accounts for 60% of all the housing forms, depending on the variations in stories through BIM-based energy simulation. For this study, four representative block types were selected, based on the multi-family floor plan, which is certified for energy performance evaluation and they were applied to the floor plan of a multi-family house that is scheduled to be built. Then BIM modeling was conducted from the fifth story to the 40th story at an intervals of 5 stories and based on the finding, energy characteristics of each block type and energy performance depending on the variations in stories were analyzed. It is considered that this would serve as objective data for block type and block story decision of energy performance-based multi-family housing.

• Journal of Environmental Health Sciences
• /
• v.26 no.4
• /
• pp.141-147
• /
• 2000

Due to a rapid in automobiles since the 1980’s, the concentration of NO, and HC has also increased along with cases of VOCs. These air pollutants have created $O_3$ concentration, which cause a harmful effect to the human health. This issue has become a subject of great public interest. For this paper, to compare the concentration of $O_3$, NO, N $O_2$ between the rural and urban area in the winter, the concentrations of each have been measuredevery hour during Jan.~Feb. 2000, 1998, respectively. To calculate the Photochemical Steady State, $\Phi$= $J_{N O_2}$[N $O_2$]/ $k_1$[NO][ $O_3$], temperature and $J_{ N O_2}$ has been determined. The NO concentration in the rural are showed at below 10 ppb while the NO concentration in the urban area showed maximum value of 90~120 ppb. But the $O_3$ concentration in both areas showed less than 30 ppb. The reason is that the N $O_2$ photodissiciation rate is low due to the temperature being below 2$^{\circ}C$ and less than 60 degrees in the solar zenith angle during the winter time, which makes the $O_3$ concentration in both areas, similar in the concentration level. N $O_2$ photodissociation rate in both ares showed maximum value of 3.0mW/$\textrm{cm}^2$. Values of $\Phi$ determined from the rural area was consistently the unity, approaching 1. But values of $\Phi$ determined from the urban was roughly higher than unity, approaching above 1or 2 for clear sky-high sun(10:00~16:00).