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Studies on the Germination Characteristics of Sesame (Sesamum indicum L.) (참깨의 발아특성(發芽特性)에 관(關)한 연구(硏究))

  • Kim, Choong Soo
    • Korean Journal of Agricultural Science
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    • v.10 no.1
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    • pp.28-60
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    • 1983
  • This study was carried out to define the effects of external factors including temperature, moisture, oxygen and light quality on the germination of sesame seeds and to investigate the change of major chemical constituents of seeds during germination. The results obtained are summarized as follows: 1. The average germination ratio was from 95.8% to 97.2% when it was tested every $5^{\circ}C$ intervals from $20^{\circ}C$ to $35^{\circ}C$ and no significant difference in germination ratio was found within $20^{\circ}C$ to $35^{\circ}C$. But the germination ratio dropped rapidly to 32.2% when seeds were germinated at $15^{\circ}C$ and the coefficient of variation become greater(77%) 2. The days required for germination ranged from 1.16 to 1. 64 at the temperatures of $35^{\circ}C$ to $25^{\circ}C$ and they were 3.07 and 10.4 at the temperatures of $20^{\circ}C$ and $15^{\circ}C$, respectively. 3. Considering the germination ratio and days needed, $15^{\circ}C$ was assumed to be the minimum temperature for germination practically and this temperature is recommended for testing low temperature tolerance of seed germination of sesame cultivars. 4. The varieties shown the highest low temperature tolerance were Shirogoma and Turkey. The next varieties shown some degree of low temperature germination were Suweon #29, Naebok and IS 58. The varieties with 70 to 80% of germination ratio were Maepo, Suweon #14, Kimpo, Moondeok, and Haenam. Among the 90 varieties tested, the varieties with comparatively high degree of low temperature tolerance were about 10%, and 70% of the low temperature tolerant varieties were domestic varieties. 5. At $12^{\circ}C$ the Shirogoma was the only variety which showed over 50% of germination ratio, 71.4% of the varieties showed less than 20% of germination ratio. When the temperature was raised to $27^{\circ}C$ 18 days after placement at $12^{\circ}C$ all the varieties showed over 90% of germination ratio within 2days. 6. The amounts of water imbibition needed for seed germination were 0.48 to 0.62 times of the seed dry weight at $25^{\circ}C$ and were significantly different among sesame cultivars. About 63% of water required for germination was imbibed in 2 hours after placement of seeds under the germination condition. 7. Under saturated moisture condition the average germination ratio was 0.42%. In the soil of which water potential was -0.4bar 64.8% of the seeds germinated and the most adequate soil water potential for sesame seed germination was about -0.4 to -5.5 bar. The germination ratio decreased as the soil water potential declined below -5.5 bar. 8. Six out of 10 varieties were not influenced by 5% of oxygen in air germination chamber, while varieties such as Yecheon, PI 158073, IS 103 and Euisangcheon showed 64 to 91% of germination under the 5% oxygen content. Under anaerobic condition, cotyledones were not emerged but only hypocotyl was emerged and elongated. The germination ratio of IS 103 decreased significantly under anaerobic condition. 9. When the seeds were dried for 24 hours after 12 hours imbibition of water, the seeds of Cheongsong did not lose their germination ability and 27.5% was germinated but Suweon #9 and Early Russian failed to germinate. However, the germination ratio of IS 103 decreased when the seed were dried 24 hours after 4 hours imbibition of water and the germination ability of IS 103 was maintained even though the seeds were dried for 24 hours after 24 hours imbibition of water. 10. During germination, sugar content of sesame seed increased rapidly and activity of ${\alpha}$-amylase increased gradually while starch content decreased significantly. The rates of increase in sugar content and enzyme activity and decrease in starch content were significantly lower at $15^{\circ}C$ compared with those at $25^{\circ}C$. 11. During germination of sesame seeds, lipid content in the seeds dropped rapidly and the activity of alkaline lipase increased significantly at early stage of germination. The rate of decrease in lipid content and increase in emzyme activity was lower at $15^{\circ}C$ than at $25^{\circ}C$. 12. Four out of 6 varieties were not affected in germination by light wave length. But Suweon #8 was inhibited in germination by 600-650nm. and IS 103 by 600 to 650nm and 500 to 550nm of light wave length. Suweon #8 showed high germination ratio under 650 to 760 nm and 500 to 560nm, and IS 103 under 400 to 470nm and complete darkness. 13. The germination ratios increased significantly in the seeds of which 1000 grain weight is heavier. When the seeds were placed at soil 4cm deep, Cheongsong and Early Russian failed to emerge their cotyledones, but Suweon #9 and IS 103 showed 32.5 and 50% cotyledone emergence, respectively. The extracts from sesame plant and soil where the sesame was cultivated previously did not affect in the-germination of sesame seeds. 14. The covering by black or transparent polyethylene films increased germination ratio compared with uncovered seeds. The covering was effective in shortening the days needed for germination and in improving the early seedling growth, number of capsules per plant and grain yield. Difference was not so seizable between the two polyethylene films but the transparent film appeared somewhat more effective than the black one. 15. Simcheon, Cheongsong. Suweon #9. PI 158073 and IS 103 showed lower rate of water absorbtion by seed during germination and Suweon #8, Suweon #26, Orotall and Euisangcheon showed high increase in seed weight after water absorbtion by seed.

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Quantitative Expression Analysis of Functional Genes in Four Dog Breeds (개의 네 품종에서 기능 유전자들에 대한 정량적 발현 분석)

  • Gim, Jeong-An;Kim, Sang-Hoon;Lee, Hee-Eun;Jeong, Hoim;Nam, Gyu-Hwi;Kim, Min Kyu;Huh, Jae-Won;Choi, Bong-Hwan;Kim, Heui-Soo
    • Journal of Life Science
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    • v.25 no.8
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    • pp.861-869
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    • 2015
  • One of the domesticated species; the dog has been selectively bred for various aims by human. The dog has many breeds, which are artificially selected for specific behaviors and morphologies. Dogs contribute their life to human as working dogs for guide, rescue, detection or etc. Working dogs requires good personality, such as gentleness, robustness and patience for performing their special duty. Many studies have concentrated on finding genetic marker for selecting the high-quality working dog. In this study, we confirmed quantitative expression patterns of eight genes (ABAT; 4-Aminobutyrate Aminotransferase, PLCB1; Phospholipase C, Beta 1, SLC10A4; Solute Carrier Family 10, Member 4, WNT1; Wingless-Type MMTV Integration Site Family, Member 1, BARX2; BarH-Like Homeobox 2, NEUROD6; Neuronal Differentiation 6, SEPT9; Septin 9 and TBR1; T-Box, Brain, 1) among brains tissues from four dog breeds (Beagle, Sapsaree, Shepherd and Jindo), because these genes were expressed and have functions in brain mostly. Specially, BARX2, SEPT9, SLC10A4, TBR1 and WNT1 genes were highly expressed in Beagle and Jindo, and Sapsaree and German Shepherd were vice versa. The biological significance of total genes was estimated by database for annotation, visualization and integrated discovery (DAVID) to determine a different gene ontology (GO) class. In these analyses, we suppose to these eight genes could provide influential information for brain development, and intelligence of organisms. Taken together, these results could provide clues to discover biomarker related to functional traits in brain, and beneficial for selecting superior working dogs.

Study on the Dissolution of Sandstones in Gyeongsang Basin and the Calculation of Their Dissolution Coefficients under CO2 Injection Condition (이산화탄소 지중 주입에 의한 경상분지 사암의 용해반응 규명 및 용해 반응상수값 계산)

  • Kang, Hyunmin;Baek, Kyoungbae;Wang, Sookyun;Park, Jinyoung;Lee, Minhee
    • Economic and Environmental Geology
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    • v.45 no.6
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    • pp.661-672
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    • 2012
  • Lab scale experiments to investigate the dissolution reaction among supercritical $CO_2$-sandstone-groundwater by using sandstones from Gyeongsang basin were performed. High pressurized cell system (100 bar and $50^{\circ}C$) was designed to create supercritical $CO_2$ in the cell, simulating the sub-surface $CO_2$ storage site. The first-order dissolution coefficient ($k_d$) of the sandstone was calculated by measuring the change of the weight of thin section or the concentration of ions dissolved in groundwater at the reaction time intervals. For 30 days of the supercritical $CO_2$-sandstone-groundwater reaction, physical properties of sandstone cores in Gyeongsang basin were measured to investigate the effect of supercritical $CO_2$ on the sandstone. The weight change of sandstone cores was also measured to calculate the dissolution coefficient and the dissolution time of 1 g per unit area (1 $cm^2$) of each sandstone was quantitatively predicted. For the experiment using thin sections, mass of $Ca^{2+}$ and $Na^+$ dissolved in groundwater increased, suggesting that plagioclase and calcite of the sandstone would be significantly dissolved when it contacts with supercritical $CO_2$ and groundwater at $CO_2$ sequestration sites. 0.66% of the original thin sec-tion mass for the sandstone were dissolved after 30 days reaction. The average porosity for C sandstones was 8.183% and it increased to 8.789% after 30 days of the reaction. The average dry density, seismic velocity, and 1-D compression strength of sandstones decreased and these results were dependent on the porosity increase by the dissolution during the reaction. By using the first-order dissolution coefficient, the average time to dissolve 1 g of B and C sandstones per unit area (1 $cm^2$) was calculated as 1,532 years and 329 years, respectively. From results, it was investigated that the physical property change of sandstones at Gyeongsang basin would rapidly occur when the supercritical $CO_2$ was injected into $CO_2$ sequestration sites.

Effect of Irrigation Period on Quality of Melon(Cucumis melo L.) (관개조건(灌漑條件)이 온실재배(溫室栽培) 메론의 수량(收量) 및 품질(品質)에 미치는 영향(影響))

  • Lee, Keyong-Bo;Kim, Sun-Kwan;Yang, Chang-Hyu;Yoo, Chul-Hyun;Chon, Jang-Hyeob;Lee, Du-Ku;So, Jae-Don
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.4
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    • pp.269-274
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    • 1994
  • An experiment was carried out to investigate the effects of irrigation period on the yield and quality of melon (Cucumis melo L.) cultivated in green house 1992~1993. Four different irrigation period was applied ; from transplanting to harvest(Irrigation I), from initial flowering to 35 days after flowering(Irrigation II), from initial flowering to 20 days after flowering(Irrigation III), from initial flowering to 5 days after flowering(Irrigation IV). The water was applied by drip irrigation when the soil water potential of 15cm depth reach at -0.5bar. The results obtained were as follows ; 1. The amount of water applied during the periods were 170.5mm, 145.0mm,126.9mm and 78.8mm for irrigation period I, II, III and IV, respectively. 2. Average evapotranspiration during the cultivation of melon was 3.31mm/day. Evapotranspiration was the highest at the period from 5days after flowering to 15days after flowering. 3. The total yield was increased with the higer amount of water applied, and the yield was in the order of Irrigation I, II, III and IV. However, the yield with economically high quality was the highest in Irrigation III. 4. The sugar content and quality of Net form were decreased with higher irrigation amount.

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Influence of Short-term Application of Abscisic Acid in Nutrient Solution on Growth and Drought Tolerance of Tomato Seedlings (토마토 육묘과정에서 단기간 ABA처리가 묘소질과 건조내성에 미치는 영향)

  • Kim, Il-Seop;Vu, Ngoc-Thang;Vo, Hoang-Tung;Choi, Ki-Young;Kim, Young Shik
    • Journal of Bio-Environment Control
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    • v.24 no.1
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    • pp.13-20
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    • 2015
  • This study was conducted to evaluate influence of short-term application of abscisic acid (ABA) in nutrient solution on growth and drought tolerance of tomato seedlings. The treatments included four ABA concentrations (0.5, 1, 2, $3mg{\cdot}L^{-1}$) and control (non-treatment) were applied to the nutrient solution in a hydroponic system. On the $5^{th}$ and $10^{th}$ day after growing in the nutrient solution containing ABA, seedlings were transferred to -5 bars of PEG-8000 in a growth chamber to induce water stress. Except for stem diameter and fresh and dry weight of root, there were no statistical differences in other growth parameters among control, 0.5 and $1mg{\cdot}L^{-1}$ of ABA treatments. Seedlings growths were strongly inhibited in nutrient solution containing 2 and $3mg{\cdot}L^{-1}$ of ABA. The root growth such as fresh and dry weigh of root, total root surface area, and average root diameter was slightly enhanced in $1mg{\cdot}L^{-1}$ of ABA treatment. The elevation of ABA concentrations in nutrient solution resulted in the decrease in transpiration rate and increase in stomatal diffusive resistance and leaf temperature of tomato seedlings. The initiations of seedling wilting after treating in -5 bars of PEG were delayed from 10 hrs in control to 30 hrs in ABA applied treatments. Additionally, the high percentages of recovered seedlings were observed in 0.5 and $1mg{\cdot}L^{-1}$ of ABA treatments after re-irrigation. Therefore, short-term application of $1mg{\cdot}L^{-1}$ of ABA in the nutrient solution stimulated the root growth and drought tolerance of tomato seedlings by delaying the start time of wilting point and enhancing the recovery after re-irrigation.

Phase Equilibria of Ionic Liquid/Organic Compound/Supercritical CO2 Systems (이온성액체/유기화합물/초임계이산화탄소계의 상평형)

  • Lim, Bang-Hyun;Kim, Jong-Won;Paek, Sang-Min;Son, Bo-Kook;Lee, Yong-Rok;Lee, Chul Soo;Lee, Huen;Ra, Chun-Sup;Shim, Jae-Jin
    • Clean Technology
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    • v.12 no.3
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    • pp.128-137
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    • 2006
  • The volume change of an ionic liquid and the phase separation behavior of room temperature ionic liquid(RTIL)/organic compound mixture in supercritical carbon dioxide were measured in a high pressure view cell. 1-Butyl-3-methylimidazolium hexafluorophosphate ([bmim][$PF_6$]) and 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][$BF_4$]) was used as ionic liquid(IL). and methanol and dimethyl carbonate were used as organic compound. For a fixed amount of [bmim][$PF_6$] the lower critical endpoint (LCEP) pressure, where the liquid phase is split, decreased as increasing the amount of organic compound. The LCEP pressure became higher as the water content of ionic liquid was higher. However, for water contents above a certain value, no LCEP was formed. LCEP appeared 1.0 MPa higher for a mixture with [bmim][$BF_4$] than with [bmim][$PF_6$]. There was almost no difference in the K-point pressures for different types of ionic liquid and for different amounts of organic liquid. When the concentration of ionic liquid([bmim][$PF_6$]) (IL/(IL+MeOH)) in the initial liquid mixture was larger than 5.9 mol% at the LCEP of the mixture, the volume of $L_1$ because larger than the volume of $L_2$. When it was smaller, however, the volume became smaller, too. The volume change of ionic liquid in the presence of carbon dioxide decreased as increasing the temperature, while it increased as increasing the pressure. For temperatures between 313.15 to 343.15K at 300 bar, it was about 123~125 % of the original volume.

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Geochemical Reactive Experimental and Modeling Studies on Caprock in the Pohang Basin (포항분지 덮개암에 대한 지화학적 반응 실험 및 모델링 연구)

  • Kim, Seon-ok;Wang, Sookyun;Lee, Minhee
    • Economic and Environmental Geology
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    • v.49 no.5
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    • pp.371-380
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    • 2016
  • This study aims to identify the mineraloical and petrographical characteristics of caprock from drilling cores of Pohang basin as a potential $CO_2$ storage site. Experiments and modeling were conducted in order to investigate the geochemical and mineralogical caprock effects of carbon dioxide. A series of autoclave experiments were conducted to simulate the interaction in the $scCO_2$-caprock-brine using a high pressure and temperature cell at $50^{\circ}C$ and 100 bar. Geochemical and mineralogical alterations after 15 days of $scCO_2$-caprock-brine sample reactions were quantitatively examined by XRD, XRF, ICP-OES investigation. Results of mineralogical studies, together with petrographic data of caprock and data on the physicochemical parameters of brine were used for geochemical modeling. Modelling was carried out using the The Geochemist's Workbench 11.0.4 geochemical simulator. Results from XRD analysis for caprock sample showed that major compositional minerals are quartz, plagioclase, and K-feldspar, and muscovite, pyrite, siderite, calcite, kaolinite and montnorillonite were included on a small scale. Results from ICP-OES analysis for brine showed that concentration of $Ca^{2+}$, $Na^+$, $K^+$ and $Mg^{2+}$ increased due to dissolution of plagioclase, K-feldspar and muscovite. Results of modeling for the period of 100 years showed that the recrystallization of kaolinite, dawsonite and beidellite, at the expense of plagioclase and K-feldspar is characteristic. Volumes of newly precipitation minerals and minerals passing into brine were balanced, so the porosity remained nearly unchanged. Experimental and modeling results indicate the interaction between caprock and $scCO_2$ during geologic carbon sequestration can exert significant impacts in brine pH and solubility/stability of minerals.

CORRELATION BETWEEN MONTHLY CUMULATIVE AURORAL ELECTROJET INDICES, DST INDEX AND INTERPLANETARY ELECTRIC FIELD DURING MAGNETIC STORMS (자기폭풍 기간 동안의 월별 누적 오로라 제트전류 지수, Dst 지수 및 행성간 전기장 사이의 상관관계)

  • Park, Yoon-Kyung;Ahn, Byung-Ho;Moon, Ga-Hee
    • Journal of Astronomy and Space Sciences
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    • v.22 no.4
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    • pp.409-418
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    • 2005
  • Magnetospheric substorms occur frequently during magnetic storms, suggesting that the two phenomena are closely associated. We can investigate the relation between magnetospheric substorms and magnetic storms by examining the correlation between AE and Dst indices. For this purpose, we calculated the monthly cumulative AU, $\mid{AL}\mid$ and $\mid{Dst}\mid$ indices. The correlation coefficient between the monthly cumulative $\mid{AL}\mid$ and $\mid{Dst}\mid$ index is found to be 0.60, while that between monthly cumulative AU and $\mid{Dst}\mid$ index is 0.28. This result indicates that substorms seem to contribute to the development of magnetic storms. On the other hand, it has been reported that the interplanetary electric field associated with southward IMF intensifies the magnetospheric convection, which injects charged particles into the inner magnetosphere, thus developing the ring current. To evaluate the contribution of the interplanetary electric field to the development of the storm time ring current belt, we compared the monthly cumulative interplanetary electric field and the monthly cumulative Dst index. The correlation coefficient between the two cumulative indices is 0.83 for southward IMP and 0.39 for northward IMF. It indicates that magnetospheric convection induced by southward IMF is also important in developing magnetic storms. Therefore, both magnetospheric substorm and enhanced magnetospheric convection seem to contribute to the buildup of magnetic storm.

The pH Reduction of the Recycled Aggregate Originated from the Waste Concrete by the scCO2 Treatment (초임계 이산화탄소를 이용한 폐콘크리트 순환골재의 중성화)

  • Chung, Chul-woo;Lee, Minhee;Kim, Seon-ok;Kim, Jihyun
    • Economic and Environmental Geology
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    • v.50 no.4
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    • pp.257-266
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    • 2017
  • Batch experiments were performed to develop the method for the pH reduction of recycled aggregate by using $scCO_2$ (supercritical $CO_2$), maintaining the pH of extraction water below 9.8. Three different aggregate types from a domestic company were used for the $scCO_2$-water-recycled aggregate reaction to investigate the low pH maintenance of aggregate during the reaction. Thirty five gram of recycled aggregate sample was mixed with 70 mL of distilled water in a Teflon beaker, which was fixed in a high pressurized stainless steel cell (150 mL of capacity). The inside of the cell was pressurized to 100 bar and each cell was located in an oven at $50^{\circ}C$ for 50 days and the pH and ion concentrations of water in the cell were measured at a different reaction time interval. The XRD and SEM-EDS analyses for the aggregate before and after the reaction were performed to identify the mineralogical change during the reaction. The extraction experiment for the aggregate was also conducted to investigate the pH change of extracted water by the $scCO_2$ treatment. The pH of the recycled aggregate without the $scCO_2$ treatment maintained over 12, but its pH dramatically decreased to below 7 after 1 hour reaction and maintained below 8 for 50 day reaction. Concentration of $Ca^{2+}$, $Si^{4+}$, $Mg^{2+}$ and $Na^+$ increased in water due to the $scCO_2$-water-recycled aggregate reaction and lots of secondary precipitates such as calcite, amorphous silicate, and hydroxide minerals were found by XRD and SEM-EDS analyses. The pH of extracted water from the recycled aggregates without the $scCO_2$ treatment maintained over 12, but the pH of extracted water with the $scCO_2$ treatment kept below 9 of pH for both of 50 day and 1 day treatment, suggesting that the recycled aggregate with the $scCO_2$ treatment can be reused in real construction sites.

The Neutralization Treatment of Waste Mortar and Recycled Aggregate by Using the scCO2-Water-Aggregate Reaction (초임계이산화탄소-물-골재 반응을 이용한 폐모르타르와 순환골재의 중성화 처리)

  • Kim, Taehyoung;Lee, Jinkyun;Chung, Chul-woo;Kim, Jihyun;Lee, Minhee;Kim, Seon-ok
    • Economic and Environmental Geology
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    • v.51 no.4
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    • pp.359-370
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    • 2018
  • The batch and column experiments were performed to overcome the limitation of the neutralization process using the $scCO_2$-water-recycled aggregate, reducing its treatment time to 3 hour. The waste cement mortar and two kinds of recycled aggregate were used for the experiment. In the extraction batch experiment, three different types of waste mortar were reacted with water and $scCO_2$ for 1 ~ 24 hour and the pH of extracted solution from the treated waste mortar was measured to determine the minimum reaction time maintaining below 9.8 of pH. The continuous column experiment was also performed to identify the pH reduction effect of the neutralization process for the massive recycled aggregate, considering the non-equilibrium reaction in the field. Thirty five gram of waste mortar was mixed with 70 mL of distilled water in a high pressurized stainless steel cell at 100 bar and $50^{\circ}C$ for 1 ~ 24 hour as the neutralization process. The dried waste mortar was mixed with water at 150 rpm for 10 min. and the pH of water was measured for 15 days. The XRD and TG/DTA analyses for the waste mortar before and after the reaction were performed to identify the mineralogical change during the neutralization process. The acryl column (16 cm in diameter, 1 m in length) was packed with 3 hour treated (or untreated) recycled aggregate and 220 liter of distilled water was flushed down into the column. The pH and $Ca^{2+}$ concentration of the effluent from the column were measured at the certain time interval. The pH of extracted water from 3 hour treated waste mortar (10 ~ 13 mm in diameter) maintained below 9.8 (the legal limit). From XRD and TG/DTA analyses, the amount of portlandite in the waste mortar decreased after the neutralization process but the calcite was created as the secondary mineral. From the column experiment, the pH of the effluent from the column packed with 3 hour treated recycled aggregate kept below 9.8 regardless of their sizes, identifying that the recycled aggregate with 3 hour $scCO_2$ treatment can be reused in real construction sites.