• The KIPS Transactions:PartD
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• v.8D no.2
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• pp.132-144
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• 2001

The broadcast environment is asymmetric communication aspect that is typically much greater communication capacity available from server to clients than in the opposite direction. In addition, most of mobile computing systems only allow the generation of read-only transactions from mobile clients for retrieving different types of information such as stock data, traffic information and news updates. Since previous concurrency control protocols, however, do not consider such a particular characteristics, the performance degradation occurs when those schemes are applied to the broadcast environment having quite a high data contention. In this paper, we propose OCC/2VTS (Optimistic Concurrency Control based on 2-Version and TimeStamp) that is most appropriate for broadcast environment. OCC/2VTS lets each client process and commit query transactions for itself by using two version data in cache. If the values of appropriate data items are not changed twice by invalidation report after a query transaction starts, the query transaction is committed safely independent of commitment of update transactions. OCC/2VTS decreases the number of informing server for the purpose of commitment. Due to broadcasting the validation reports including updated recent values, it reduces the opportunity of requesting a recent data values of server as well. As a result, OCC/2VTS makes full use of the asymmetric bandwidth. It also improves transaction throughput by increasing the query transaction commit ratio as much as possible.

• KIPS Transactions on Computer and Communication Systems
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• v.6 no.9
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• pp.385-396
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• 2017

Many IoT platforms have been proposed for various IoT devices, from low-end to high-end performance. We previously proposed a new IoT platform called MinT that supports the operation of the sensing devices and network communication. In the proposed platform, the things can flexibly connect to each other and efficiently share their information. Most IoT platforms, including the MinT, support thread pooling to quickly process requests. However, using a thread pool with a fixed thread count can cause network delay and inefficient energy consumption. In this paper, we propose an enhanced method to manage the thread pool efficiently by adjusting the number of threads every cycle to regulate the device's performance. In particular, we aim to improve the performance of the Interaction Thread Pool Group, which is responsible for analyzing, processing, and re-transmitting the received packets. The experiment shows that the improved method increases the average throughput by approximately 25% compared to the existing platforms. Finally, using the proposed method, the MinT can reduce the transmission delay and energy consumption of devices in the IoT environment.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.166-176
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• 2020

Objective: An experiment was conducted to determine the effects of L-arginine (L-Arg) and N-carbamoylglutamic acid (NCG) on the growth, metabolism, immunity and community of cecal bacterial flora of weanling and young rabbits. Methods: Eighteen normal-grade male weanling Japanese White rabbits (JWR) were selected and randomly divided into 6 groups with or without L-Arg and NCG supplementation. The whole feeding process was divided into weanling stage (day 37 to 65) and young stage (day 66 to 85). The effects of L-Arg and NCG on the growth, metabolism, immunity and development of the ileum and jejunum were compared via nutrient metabolism experiments and histological assessment. The different communities of cecal bacterial flora affected by L-Arg and NCG were assessed using high-throughput sequencing technology and bioinformatics analysis. Results: The addition of L-Arg and NCG enhanced the growth of weanling and young rabbit by increasing the nitrogen metabolism, protein efficiency ratio, and biological value, as well as feed intake and daily weight gain. Both L-Arg and NCG increased the concentration of immunoglobulin A (IgA), IgM, and IgG. NCG was superior to L-Arg in promoting intestinal villus development by increasing villus height, villus height/crypt depth index, and reducing the crypt depth. The effects of L-Arg and NCG on the cecal bacterial flora were mainly concentrated in different genera, including Parabacteroides, Roseburia, dgA-11_gut_group, Alistipes, Bacteroides, and Ruminococcaceae_UCG-005. These bacteria function mainly in amino acid transport and metabolism, energy production and conversion, lipid transport and metabolism, recombination and repair, cell cycle control, cell division, and cell motility. Conclusion: L-Arg and NCG can promote the growth and immunity of weanling and young JWR, as well as effecting the jejunum and ileum villi. L-Arg and NCG have different effects in the promotion of nutrient utilization, relieving inflammation and enhancing adaptability through regulating microbial community.

• Journal of Life Science
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• v.31 no.4
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• pp.410-417
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• 2021

Next-generation sequencing (NGS) is a high-throughput technique for sequencing large numbers of DNA fragments that are prepared from a genome. This sequencing technique has been used to elucidate whole genome sequences of living organisms and to analyze complementary DNA (cDNA) or chromatin immunoprecipitated DNA (ChIPed DNA) at the genome level. After NGS, the use of proper tools is important for processing and analyzing data with reasonable parameters. However, handling large-scale sequencing data and programing for data analysis can be difficult. The Galaxy platform, a public web service system, provides many different tools for NGS data analysis, and it allows researchers to analyze their data on a web browser with no deep knowledge about bioinformatics and/or programing. In this study, we explain the procedure for preparing chromatin immunoprecipitation-sequencing (ChIP-seq) libraries and steps for analyzing ChIP-seq data using the Galaxy platform. The data analysis steps include the NGS data upload to Galaxy, quality check of the NGS data, premapping processes, read mapping, the post-mapping process, peak-calling and visualization by window view, heatmaps, average profile, and correlation analysis. Analysis of our histone H3K4me1 ChIP-seq data in K562 cells shows that it correlates with public data. Thus, NGS data analysis using the Galaxy platform can provide an easy approach to bioinformatics.

• Journal of the Korea Society for Simulation
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• v.18 no.2
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• pp.29-38
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• 2009

In the computing environment with heterogeneous resources, a job scheduling model is necessary for effective resource utilization and high-speed data processing. And, the job scheduling model has to cope with a dynamic change in the condition of resources. There have been lots of researches on resource estimation methods and heuristic algorithms about how to distribute and allocate jobs to heterogeneous resources. But, existing researches have a weakness for system compatibility and scalability because they do not support the standard language. Also, they are impossible to process jobs effectively and deal with a variety of computing situations in which the condition of resources is dynamically changed in real-time. In order to solve the problems of existing researches, this paper proposes a semantic computing-based dynamic job scheduling model that defines various knowledge-based rules for job scheduling methods adaptable to changes in resource condition and allocate a job to the best suited resource through inference. This paper also constructs a resource ontology to manage information about heterogeneous resources without difficulty as using the OWL, the standard ontology language established by W3C. Experimental results shows that the proposed scheduling model outperforms existing scheduling models, in terms of throughput, job loss, and turn around time.

• KSBB Journal
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• v.22 no.5
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• pp.297-304
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• 2007

For large and rapid screening of high-yielding mutants of lovastatin produced by filamentous fungal cells of Aspergillus terreus, one of the most important stage is to test as large amounts of mutated strains as possible. For this purpose, we intended to develop a miniaturized cultivation method using $7m{\ell}$ culture tube instead of traditional $250m{\ell}$ flask (working volume $50m{\ell}$). For obtaining large amounts of conidiospores to be used as inoculums for miniaturized cultures, 4 components i.e., glucose, sucrose, yeast extract and $KH_2PO_4$ were intensively investigated, which had been observed to show positive effect on enhancement of spore production through Plackett-Burman design experimet. When optimum concentrations of these components that were determined through application of response surface method (RSM) based on central composite design (CCD) were used, maximum spore numbers amounting to $1.9\times10^{10}$ spores/plate were obtained, resulting in approximately 190 fold increase as compared to the commonly used PDA sporulation medium. Using the miniaturized cultures, intensive strain development programs were carried out for screening of lovastatin high-yielding as well as highly reproducible mutants. It was observed that, for maximum production of lovastatin, the producers should be activated through 'PaB' adaptation process during the early solid culture stage. In addition, they should be proliferated in condensed filamentous forms in miniaturized growth cultures, so that optimum amounts of highly active cells could be transferred to the production culture-tube as reproducible inoculums. Under these highly controlled fermentation conditions, compact-pelleted morphology of optimum size (less than 1 mm in diameter) was successfully induced in the miniaturized production cultures, which proved essential for maximal utilization of the producers' physiology leading to significantly enhanced production of lovastatin. As a result of continuous screening in the miniaturized cultures, lovastatin production levels of the 81% of the daughter cells derived from the high-yielding producers turned out to be in the range of 80%$\sim$120% of the lovastatin production level of the parallel flask cultures. These results demonstrate that the miniaturized cultivation method developed in this study is efficient high throughput system for large and rapid screening of highly stable and productive strains.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.2
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• pp.1200-1206
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• 2015

The major target for drug discovery, G-protein coupled receptor (GPCR) is involved in many physiological activities and related to various diseases and disorders. Among experimental techniques relating to the GPCR drug discovery process, various cell-based screening methods are influenced by cell conditions used in the overall process. Recently, the utilization of frozen cells is suggested in terms of reducing data variation and cost-effectiveness. The aim of this study is to evaluate various conditions in cell freezing such as temperature conditions and storage terms. The stable cell lines for calcium sensing receptor and urotensin receptor were established followed by storing cultured cells at $-80^{\circ}C$ up to 4 weeks. To compare with cell stored at liquid nitrogen, agonist and antagonist responses were recorded based on the luminescence detection by the calcium induced photoprotein activation. Cell signals were reduced as the storage period was increased without the changes in $EC_{50}$ and $IC_{50}$ values $EC_{50}:3.46{\pm}1.36mM$, $IC_{50}:0.49{\pm}0.15{\mu}M$). In case of cells stored in liquid nitrogen, cell responses were decreased comparing to those in live cells, however changes by storage periods and significant variations of $EC_{50}/IC_{50}$ values were not detected. The decrease of cell signals in various frozen cells may be due to the increase of cell damages. From these results, the best way for a long-term cryopreservation is the use of liquid nitrogen condition, and for the purpose of short-term storage within a month, $-80^{\circ}C$ storage condition can be possible to adopt. As a conclusion, the active implementation of frozen cells may contribute to decrease variations of experimental data during the initial cell-based screening process.