• 한국환경보건학회지
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• 제36권1호
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• pp.52-60
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• 2010

High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin $B_1$ (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/ MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378 samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2, walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid, accurate and cost-effective method for the detection of aflatoxins in large number of samples.

• Child Health Nursing Research
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• 제15권2호
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• pp.127-135
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• 2009

Purpose: This cross-sectional study was designed to identify anger-expression types in late school-age children and to investigate the relation of anger and the anger-expression type to their eating disorders. Method: Two hundred fifty four children in elementary school were recruited from 14 to 25, April, 2008. The data were analyzed by using descriptive statistics, cluster analysis, t-test, ANOVA, and Tukey's multiple comparison test. Result: Three anger-expression types in late school-age children were found; Anger-in/out, Anger-control, and Low anger-expression types. Children frequently using the anger-out/in type among the three types and with a higher trait anger reported higher eating disorders. Particularly trait anger and unhealthy anger expression type were linked to eating disorders. Conclusion: This study suggests that a specific anger management program needs to be developed for late school-age children with high trait anger and frequently using the anger out/in expression type and Anger-control type than low anger-expression types. Further research needs to be done with large samples and discussed in terms of the role of gender in eating disorders.

• 농촌계획
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• 제28권3호
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• pp.35-47
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• 2022

The study is to analyze the role of highschool youth group in a rural regeneration project. The process of youth group becoming subjective in resident participation plan was classified into isolation reduction, self-awareness, perspective-taking, subjectivity practice, and opinion expression. Isolation reduction is a stage in which youth group is gradually drawn into the community from a limited society of home and school, self-awareness is a stage in which they discover their thoughts, and view perspective-taking is a stage in which they objectify themselves and surroundings through communication with other members of the region, social experiment was analyzed as a practice of subjectivity that experiences leadership guiding the local community independently, and expressing opinions is a stage of representing the region and giving responsibility for the specific issue of the implementation plan. The study is also an analysis of how residents committee, local governments, and local research institutes perform both regeneration and community revitalization in rural areas. Therefore, the analysis of the cooperative organization of these institutions was conducted simultaneously. The leadership program was effectively linked to the project of rural regeneration. Since high school students themselves are family members of the local people, the process of accepting the project is unaffected, and the feasibility of the project is also increased, such as forming a positive atmosphere for the project and easing resistance to minority opinions.

• International Journal of Oral Biology
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• 제33권3호
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• pp.77-82
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• 2008

Innate immune response is initiated by the recognition of unique microbial molecular patterns through pattern recognition receptors (PRRs). The purpose of this study is to dissect the expression of various PRRs in gingival epithelial cells of differentiated versus undifferentiated states. Differentiation of immortalized human gingival epithelial HOK-16B cells was induced by culture in the presence of high $Ca^{2+}$ at increased cell density. The expression levels of various PRRs in HOK-16B cells were examined by realtime reverse transcription polymerase chain reaction (RTPCR) and flow cytometry. In addition, the expression of human beta defensins (HBDs) was examined by real time RT-PCR and the amounts of secreted cytokines were measured by enzyme linked immunosorbent assay. In undifferentiated HOK-16B cells, NACHT-LRR-PYDcontaining protein (NALP) 2 was expressed most abundantly, and toll like receptor (TLR) 2, TLR4, nucleotide-binding oligomerization domain (NOD) 1, and NOD2 were expressed in substantial levels. However, TLR3, TLR7, TLR8, TLR9, ICE protease-activating factor (IPAF), and NALP6 were hardly expressed. In differentiated cells, the levels of NOD2, NALP2, and TLR4 were different from those in undifferentiated cells at RNA but not at protein levels. Interestingly, differentiated cells expressed the increased levels of HBD-1 and -3 but secreted reduced amount of IL-8. In conclusion, the repertoire of PRRs expressed by gingival epithelial cells is limited, and undifferentiated and differentiated cells express similar levels of PRRs.

• 한국식품영양학회지
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• 제30권2호
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• pp.297-304
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• 2017

Although adolescents' obesity prevalence increases recently in Korea due to Westernized dietary life, according to social and economic development, and physical activity decline, distorted body image and improper dietary habits are prevalent, because of excessive obsession with weight control, deriving from psychological impacts including appearance supremacy. This study conducted a survey on health oriented awareness and attitude related with dietary habits targeting adolescents, such as high school students and college students, younger than 24, from May 11 to May 29, 2015. In this study, 280 questionnaire copies were collected out of 300 distributed questionnaire copies, and used 269 copies as analysis data, except 11 copies of which responses were inadequate. The results of this study are as follows: First, the high school students showed higher dietary regularity than the college students. Especially, 95.5% of the college students showed very high irregularity of lunch, compared with just 4.5% of the high school students. Second, the adolescents showed distorted perceived body image in comparison with body mass index (BMI). As a result of examining BMI relationship, according to one's own perception on body type, 28.6% of the respondents perceived themselves fat, despite normal BMI, and 40.4% of the respondents perceived themselves normal, despite low BMI. Third, health behavior activities level was higher, as the regularity of breakfast (B=1.093), lunch, (B=0.650), and dinner (B=0.765) was higher. The variable affecting the most ( ${\beta}=0.372$) was the regularity of breakfast. As interest in weight control was higher, health behavior activities was lower. Because over-interest in weight control may be linked with improper dietary habits or weight control, a caution is needed. The results of this study are expected to be utilized as the basic data for the policy and health-oriented program development to improve adolescents' health behavior practice.

• 한국가정과교육학회지
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• 제24권3호
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• pp.191-208
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• 2012

본 연구는 청소년의 윤리적 소비교육의 실효성을 극대화할 수 있는 방안을 탐색해 보고자, 중고등학생 587명을 대상으로 가정 학교 사회에서의 윤리적 소비 교육 경험과 윤리적 소비행동에 대해 살펴보았다. 연구결과 첫째, 청소년들의 윤리적 소비 교육 경험들이 대체적으로 높지 않게 나타났으며, 이 중 학교소비자교육에 대한 교육 경험이 상대적으로 높게 나타났다. 윤리적 소비행동 영역에서는 녹색소비를 중심으로 한 미래세대를 위한 소비자교육이 많이 이루어지고 있었다. 둘째, 학교 프로그램에서는 행사 및 봉사활동에 참여함으로써, 사회 프로그램에서는 벼룩시장 참여를 통해 윤리적 소비를 많이 접하고 있었다. 그리고 인터넷을 통한 윤리적 소비 교육의 접촉 빈도가 높은 경향을 나타내고 있다. 셋째, 청소년 소비자의 윤리적 소비 행동 수준이 전반적으로 높지 않으므로, 윤리적 소비 교육이 지속적으로 행해져야 한다. 특히 동시대 인류를 위한 소비 교육이 더 요구되어진다. 넷째, 청소년 소비자의 윤리적 소비행동에 영향을 미치는 요인으로는 여성인 경우, 가정소비자교육과 학교소비자교육을 많이 받을수록, 학년이 저학년일수록 더욱 윤리적인 소비행동을 하는 것으로 나타났다. 이와 같이 가정 학교 사회에서의 윤리적 소비교육의 중요성이 나타나므로, 서로 연계하여 청소년 스스로 경험을 통해 터득할 수 있도록 실생활과 연관된 교육방법과 교육내용의 개발이 이루어져야 할 것이다.

• 한국식품과학회지
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• 제48권6호
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• pp.555-559
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• 2016

국내에서 유통되는 조리식품 6종류, 151점의 시료를 수집하여 LC-MS/MS를 이용해 PFCs 잔류 수준을 측정하였다. 총 12종의 PFCs를 분석하였으며, PFBS를 제외한 PFCs의 회수율은 82.2-119.4%였으며, 정밀도는 2.2-28.9%였다. PFCs물질별로는 PFPeA (84.1%)와 PFOA (82.8%) 검출률이 높았고, PFOS의 검출률은 29.1%였으며, 대부분의 시료에서 PFOA가 PFOS보다 잔류 수준이 높은 경향을 보였다. 시료의 총 PFCs의 평균 농도는 $1.68{\pm}1.79ng/g$이었으며, PFPeA와 PFOA의 비중이 높았다.

• Nutrition Research and Practice
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• 제17권5호
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• pp.827-843
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• 2023

BACKGROUND/OBJECTIVES: Mitochondrial DNA leakage leads to inflammatory responses via endosome activation. This study aims to evaluate whether the perennial grass water extract (Pogonatherum panicum) ameliorate mitochondrial DNA (mtDNA) leakage. MATERIALS/METHODS: The major bioactive constituents of P. paniceum (PPW) were investigated by high-performance liquid chromatography, after which their antioxidant activities were assessed. In addition, RAW 264.7 macrophages were stimulated with lipopolysaccharide, resulting in mitochondrial damage. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to examine the gene expression and cytokines. RESULTS: Our results showed that PPW extract-treated activated cells significantly decrease reactive oxygen species and nitric oxide levels by reducing the p2^phox and iNOS expression and lowering cytokine-encoding genes, including IL-6, TNF-α, IL-1β, PG-E2 and IFN-γ relative to the lipopolysaccharide (LPS)-activated macrophages. Furthermore, we observed that LPS enhanced the mtDNA leaked into the cytoplasm, increasing the transcription of Tlr9 and signaling both MyD88/Irf7-dependent interferon and MyD88/NF-κb p65-dependent inflammatory cytokine mRNA expression but which was alleviated in the presence of PPW extract. CONCLUSIONS: Our data show that PPW extract has antioxidant and anti-inflammatory activities by facilitating mtDNA leakage and lowering the Tlr9 expression and signaling activation.

• 한국지역사회생활과학회지
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• 제19권3호
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• pp.335-351
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• 2008

Adequate nutrients intake and regular eating habit are so important for the students to develop physical and mental health, and they contribute significantly for the development of intellectual capacity. The intake of simple sugar contained in the processed foods is known to have been related not only with such physical problems as obesity and diabetes but also with hyperactivity during growing period. This study analysed the effects of carbohydrate processed snacks on the middle school students' emotional intelligence and intended to demonstrate the correlation and the influence of the foods. The subjects of this study were 476 the 1st grade middle school students sampled from two public middle schools of Gyeonggi Province, Korea. Questionnaire contained 47 questions including the kinds of sugar containing processed foods, the frequency of intake, the frequency of normal diet, and the emotional test. All data obtained in this study were processed by SAS 8.2 program. 77.9% of the students had snacks more than once a day and the favored foods were in the order of bread, cookies, fruit, milk and dairy products, flour foods, ramen and ice cream. Less of the sugar contained processed snacks consumed high emotional intelligence showed(p<0.001). And low intake of carbohydrate processed snacks linked with having more regular meals and the higher levels of emotional intelligence. There was no gender difference in consuming simple sugar containing processed foods, however, out of these foods, carbonated drinks, ramen and pizza affected significantly the emotional intelligence of the male students. It was found that sugar contained processed snacks affect the eating habits like meal skipping and the emotional intelligence. It is determined that the effects of carbohydrate processed snacks on emotional intelligence can not be denied.

• 한국가정과교육학회지
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• 제32권4호
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• pp.81-101
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• 2020

이 연구는 중학교 가정교과 주생활 영역에서 학교 공간 디자인을 주제로 하여 과정중심평가 프로그램을 개발하고 실행하는 데 목적이 있다. 이에 따라 ADDIE 교수설계 모형에 따라 관련 문헌 및 선행연구 분석을 통해 학교 공간 디자인을 주제로 한 과정중심평가 프로그램을 개발하고, 총 93명의 학생을 대상으로 실행한 후 학생들에게 프로그램 실행 평가를 실시하였다. 이 연구의 결과는 다음과 같다. 첫째, 2015 개정 가정과 교육과정 분석을 통해 학교 공간 디자인 프로그램 평가 계획을 수립하고, 평가 과제를 개발하였다. 이에 따라 채점기준을 마련하고 학생용 평가 자료 8개, 교사용 평가 자료 2개, 총 9차시의 수업·평가 과정안을 개발하였다. 전문가 타당도 검증을 실시한 결과, 모든 문항이 평균 3.6점~4점(4점 만점)으로 내용타당도와 내용구성이 적절하다고 검증받았다. 둘째, 학교 공간 디자인 프로그램을 실행하고 학생들에게 설문조사를 실시한 결과 학교 공간 디자인 수업, 과정중심평가, 관심 및 흥미 3가지 영역 모두 평균 4.12~4.27점(5점 만점)으로 높게 나타났다. 설문 조사와 심층면접 결과를 종합해볼 때 프로그램이 학교 공간 디자인에 대한 새로운 학습기회를 제공하고, 학생들은 제시한 성취기준에 도달할 수 있었으며 자기평가, 동료평가 및 교사의 피드백은 학습 과정 중에 학생들에게 긍정적인 영향을 주어 학습 활동에 적극적으로 참여할 수 있게 된다는 것을 확인하였다. 연구 대상이 일부 중학생들으로 연구가 이루어져서 연구 결과를 일반화하기에는 한계가 있으며 후속 연구로 연구 대상 및 평가의 영역, 학교 공간 디자인 수업의 확대를 제언하였다. 이 연구는 가정 교과에서 활용할 수 있는 과정중심평가 프로그램을 개발하여 이론과 실천의 토대를 마련하였으며, 가정과 교육의 주생활 내용 요소를 중심으로 교사와 학생이 학교 공간 디자인에 주도적으로 참여하게 하는 가능성을 제시한 것에 의의가 있다.