Kim, Jong-Gu;Lee, Kyeong-Bo;Lee, Sang-Bok;Lee, Deog-Bae;Kim, Seong-Jo
Korean Journal of Soil Science and Fertilizer
/
v.33
no.6
/
pp.416-431
/
2000
The objective of this study was to determine the effects of various kinds of composts on the change of soil chemical properties in upland soils. Field experiments were conducted in the loam and sandy loam soils. Various kinds of composts such as poultry manure compost(PMC), cow manure compost(CMC), human excrement sludge(HES), and food industrial sludge compost(FISC) were applied annually at rates of 0, 40, and $80Mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : The continuous application of human excrement sludge decreased soil pH up to 4.4~5.0, while other compost treatments increased soil pH compared with control plot. The EC increased initially and showed their maximum values at 20days after compost application, and then decreased up to 40 days, thereafter kept a certain level. The available phosphorous accumulated at 0~20cm depth in loam soil, and 0~50cm in sandy loam soil. Annual accumulation rates were 17% higher in sandy loam soil than loam soil. The more compost application rates and times, the higher base saturation percentage increased in upland soils. Four year's application at a rate of $80Mg\;ha^{-1}$ per year increased the base saturation percentage to 87~97% compared with 45% at control plot in the loam soil. While in sandy loam soil only three year's application of same rate increased the base saturation percentage to 81~92% compared with 30.4% at control plot. The average annual increasing rate of base saturation percentage at the same application rates of composts were higher in sandy loam soil by 2.0~3.7 times than in loam soil. The application of compost increased the exchangeable Ca, Mg, and K contents of soils by 2, 2~3, and 3~5 times, respectively, compared with the control. The contents of exchangeable cations were high in surface soil, and decreased with increase of soil depths. In the case of heavy metal content, there were no difference at the application of PMC and CMC but Ni, Fe, Zn, Cu was increased a little when the HES applied, and Ni and Cr was increased application with FISC.
Park, Joo-Hung;Lee, Jeong-Min;Lee, Eun-Jin;Hwang, Won-Bhin;Kim, Da-Jeong
Molecules and Cells
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v.41
no.4
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pp.290-300
/
2018
Using an in vitro model of intestinal organoids derived from intestinal crypts, we examined effects of indole-3-carbinol (I3C), a phytochemical that has anticancer and aryl hydrocarbon receptor (AhR)-activating abilities and thus is sold as a dietary supplement, on the development of intestinal organoids and investigated the underlying mechanisms. I3C inhibited the in vitro development of mouse intestinal organoids. Addition of ${\alpha}$-naphthoflavone, an AhR antagonist or AhR siRNA transfection, suppressed I3C function, suggesting that I3C-mediated interference with organoid development is AhR-dependent. I3C increased the expression of Muc2 and lysozyme, lineage-specific genes for goblet cells and Paneth cells, respectively, but inhibits the expression of IAP, a marker gene for enterocytes. In the intestines of mice treated with I3C, the number of goblet cells was reduced, but the number of Paneth cells and the depth and length of crypts and villi were not changed. I3C increased the level of active nonphosphorylated ${\beta}$-catenin, but suppressed the Notch signal. As a result, expression of Hes1, a Notch target gene and a transcriptional repressor that plays a key role in enterocyte differentiation, was reduced, whereas expression of Math1, involved in the differentiation of secretory lineages, was increased. These results provide direct evidence for the role of AhR in the regulation of the development of intestinal stem cells and indicate that such regulation is likely mediated by regulation of Wnt and Notch signals.
Objectives: Hydrogen peroxide ($H_2O_2$) surface treatment of fiber posts has been reported to increase bond strength of fiber posts to resin cements. However, residual oxygen radicals might jeopardize the bonding procedure. This study examined the effect of three antioxidant agents on the bond strength of fiber posts to conventional and self-adhesive resin cements. Materials and Methods: Post spaces were prepared in forty human maxillary second premolars. Posts were divided into five groups of 8 each: G1 (control), no pre-treatment; G2, 10% $H_2O_2$ pre-treatment; G3, G4 and G5. After $H_2O_2$ application, Hesperidin (HES), Sodium Ascorbate (SA) or Rosmarinic acid (RA) was applied on each group respectively. In each group four posts were cemented with Duo-Link conventional resin cement and the others with self-adhesive BisCem cement. Push-out test was performed and data were analyzed using 2-way ANOVA and tukey's post-hoc test (${\alpha}=0.05$). Results: There was a statistically significant interaction between the cement type and post surface treatment on push-out bond strength of fiber posts (p < 0.001, F = 16). Also it was shown that different posts' surface treatments significantly affect the push-out bond strength of fiber posts (p = 0.001). $H_2O_2$ treated posts (G2) and control posts (G1) cemented with Duo-link showed the highest ($15.96{\pm}5.07MPa$) and lowest bond strengths ($6.79{\pm}3.94$) respectively. Conclusions: It was concluded that $H_2O_2$ surface treatment might enhance the bond strength of fiber posts cemented with conventional resin cements. The effect of antioxidants as post's surface treatment agents depends on the characteristics of resin cements used for bonding procedure.
Using the spectral data in the 3700 to $10050{\AA}$ wavelength range secured with the Hamilton Echelle Spectrograph (HES) at the Lick observatory, we have investigated the expansion velocities and the physical conditions of the elliptical planetary nebula NGC 6803. Various forbidden and permitted lines, e.g. HI, HeI, HeII, [OIII], [NII], [ArIII], and [SII], indicate complicated but systematic physical conditions variation: electron temperatures $T_{\varepsilon}\;{\sim}\;9000$ - 11000 K and electron number densities $N_{\varepsilon}\;{\sim}\;2000$ - $9000\;cm^{-3}$. The line profile analysis of these ions also indicates the systematic change or the acceleration of the expansion velocities in the range of 10 - $22\;km\;s^{-1}$. We show that the velocity gradient and physical condition found in various ions are closely related to the prolate ellipsoidal structure of NGC 6803. The expansion velocity and the ionic abundance of $O^{2+}$ were derived based on the OII and [OIII] lines. In spite of the discrepancy of ionic abundances derived by the two cases and their line profiles, the expansion velocities of them agree well. We find that the ratios of the red to blue line component of the HeII & OII lines are different from those of the [OIII] or other forbidden lines that indicates a possible involvement of emission of HeII & OII lines. This subtle difference and the different physical condition of the lines are likely to be caused by the elongated geometry and the latitude dependence of the emission zone.
The objective of this study was to determine the effects of various kinds of composts on the amount of maximum composts in upland soils. Field experiments were conducted in the loam and sandy loam soils. Various kinds of composts such as poultry manure compost(PMC), cow manure compost(CMC), human excrement sludge(HES), and food industrial sludge compost(FISC) were applied annually at rates of 0, 40, and $80Mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : Maize dry matter production was related linearly positively with application amount of compost, but soybean dry matter production was showed an apex at $60Mg\;ha^{-1}$, maize produced 4.7 times dry matter as compared to soybean. Rate of nitrogen utilization of maize were 21~31% and that of soybean were 8~19%, rate of phosphorous utilization of maize were 5~7% and that of soybean were l~2%. On the basis of biomass maximum application rates of poultry manure compost were $39{\sim}47Mg\;ha^{-1}$ for maize cultivation and $8{\sim}13Mg\;ha^{-1}$ for soybean cultivation. On the other hand those amounts estimated by nitrogen requirement of crops were 39~47 and $8{\sim}13Mg\;ha^{-1}$ for maize and soybean cultivation, respectively. Based on phosphorous requirement of crops those were 29 and $14Mg\;ha^{-1}$ for maize and soybean cultivation, respectively. Considering the upper limit of the base saturation percentage to be 80%, maximum application rates were 63~93 and $49{\sim}69Mg\;ha^{-1}$ in loam and sandy loam soil, respectively.
Notch1 has been reported to be highly expressed in triple-negative and other subtypes of breast cancer. Mutant p53 (R280K) is overexpressed in MDA-MB-231 triple-negative human breast cancer cells. The present study aimed to determine whether the mutant p53 can be a potent transcriptional activator of the Notch1 in MDA-MB-231 cells, and explore the role of this mutant p53-Notch1 axis in curcumin-induced apoptosis. We found that curcumin treatment resulted in an induction of apoptosis in MDA-MB-231 cells, together with downregulation of Notch1 and its downstream target, Hes1. This reduction in Notch1 expression was determined to be due to the decreased activity of endogenous mutant p53. We confirmed the suppressive effect of curcumin on Notch1 transcription by performing a Notch1 promoter-driven reporter assay and identified a putative p53-binding site in the Notch1 promoter by EMSA and chromatin immunoprecipitation analysis. Overexpression of mutant p53 increased Notch1 promoter activity, whereas knockdown of mutant p53 by small interfering RNA suppressed Notch1 expression, leading to the induction of cellular apoptosis. Moreover, curcumin-induced apoptosis was further enhanced by the knockdown of Notch1 or mutant p53, but it was decreased by the overexpression of active Notch1. Taken together, our results demonstrate, for the first time, that Notch1 is a transcriptional target of mutant p53 in breast cancer cells and suggest that the targeting of mutant p53 and/or Notch1 may be combined with a chemotherapeutic strategy to improve the response of breast cancer cells to curcumin.
The objective of this study was to determine the effects of various kinds of composts on the change of soil physical properties and microorganism in upland soils. Field experiments were conducted in the loam and sandy loam soils, while the clay loam and sandy loam soils were used for laboratory experiments. Various kinds of composts such as poultry manure compost(PMC). cow manure compost(CMC). human excrement sludge(HES), and food industrial sludge compost(FISE) were applied annually at rates of 0, 40, and $80Mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : Bulk density of loam soil decreased with compost application to $1.07{\sim}1.32Mg\;m^{-3}$ compared with $1.49Mg\;m^{-3}$ of control plot, while in sandy loam soil it decreased to $1.00{\sim}1.20Mg\;m^{-3}$ compared with $1.25Mg\;m^{-3}$ of control plot. Bulk density of soil was decreased according to maize cultivation compared with bare control, but soybean cultivation was similar. Population of organic material decomposing microorganisms was increased rapidly at the initial incubation stage at $25^{\circ}C$, and increased more sensitively at the loam soil than sandy loam soil. In the case of the change of microorganisms associated with nitrogen circulation, ammonia oxidizing bacteria was more at the initial incubation stage, and denitrifying bacteria was more at the initial incubation stage, and denitrifying bacteria increased until 1~4 weeks after incubation and increased more at the loam soil than sandy loam soil.
Longitudinal distribution of the fish fauna of the 20 main channel and tributary sites in the Nakdong River was investigated from July 1999 to December 2000. A total of 4,791 individuals of fisHes were collected from three to seven sampling programs of all study sites and 48 species of 17 families were collected. Dominant family was Cyprinidae (25 species, relative abundance (RA): 91.8%). Zacco platypus (RA: 18.0%) was found to be the most abundant inhabitant. Subdominant species were Opsariichthys bidens (17,1%), Squalidus chankaensis tsuchigae (13.2%), Hemibarbus labeo (9.9%), and Z. temmincki (8.2%). Among the total species, 12 species were identified as endemic species (4 families, 24.5% of 1,172 individuals). Four exotic fishes were collected (Carassius cuvieri, 25 indv.; Micropterus salmoides, 214 indv.; Lipomis macrochirus, 14 indv.; Oreochromis niloticus, 6 indv.). M. salmoides was distributed in mid to lower part of the Nakdong River. As the result of cluster analysis, sampling sites were divided into three groups (upper, middle and lowerpart). Site below Gumi (St. 10) were considered as the lower part of the Nakdong River. Combined with previous surveys, about 80 species are present in the main channel and tributaries of the Nakdong River. Since the middle of 1990s, population density of the M. salmoides rapidly increased in the stagnant lower part of the river(RA 8.8% in lower 5 sites). The feeding strategy of M. salmoides was "generalized" on Daphnia sp. (<100 mm), and "specialization" about Cladocera (<150 mm) and Insecta(<200 mm).
Inflammation scan using radiolabelled leukocytes has high sensitivity and specificity. Several methods for labelling leukocytes have been evaluated using P-32 diisopropyl fluorophosphate (DFP-32), H-3 thymidine, Cr-51 chromate, Ga-67 citrate and Tc-99m-sulfur colloid. In-111-oxine has proved so far to be the most reliable agent for labelling leukocytes. In-111-oxine is, however, expensive, not easily available when needed, and its radiation dose to leukocytes is relatively high. Moreover, resolution of the resultant image is relatively poor. Tc-99m is still the agent of choice because of, as compared with the indium, its favorable physical characteristics, lower cost and availability. Now the technique for labelling the leukocytes with technetium is successfully obtained using the lipophilic HAPAO with higher efficiency for granulocytes than for other cells. With this technique it is possible to label leukocytes in plasma to improve the viability of the leukocytes. Inflammation scan using Tc-99m-HMPAO has been evaluated in several laboratories, and difference in methods for separation and labelling accounts for difference in efficiency, viability and biodistribution of the labelled leukocytes. We performed inflammation scan using leukocytes labelled with Tc-99m-HMPAO in three dogs 24 hours after inoculation of live E. Coli and A. Aureus in their right abdominal wall. We separated mixed leukocytes by simple sedimentation using 6% hetastarch (HES) and labelled the leukocytes with Tc-99m-HMPAO in 20% cell free plama diluted with phosphate buffer solution(Fig. 1). Uptake was high in the liver and spleen but is was minimal in the lungs on whole body scan. Kidneys and intestine showed minimal activity although it was high in the urinary bladder(Fig. 2). Uptake of labelled leukocytes in the inflammation site was do(mite on 2 hour-postinjection scan and abscess was clearly delineated on 24 hour-delayed scan with high target-to-nontarget ratio(Fig. 3, 4). Inflammation scan using mixed leukocytes labelled with Tc-99m-HMPAO is very sensitive and specific in early detection of inflammation.
Kim, Jong-Gu;Lee, Kyeong-Bo;Kim, Jae-Duk;Han, Sang-Su;Kim, Seong-Jo
Korean Journal of Soil Science and Fertilizer
/
v.33
no.6
/
pp.432-445
/
2000
The objective of this study was to determine the effects of various kinds of composts on the change of nutrition loss in upland soils. Field experiments were conducted in the loam and sandy loam soils, while the clay loam and sandy loam soils were used for laboratory experiments. Various kinds of composts such as poultry manure compost(PMC), cow manure compost(CMC), human excrement sludge(HES), and food industrial sludge compost(FISC) were applied annually at rates of 0, 40, and $80mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : The loss of nutrients in the form of cation and anion by run-off water increased with the increase of compost application rate. Compared with bare soils, maize cultivation decreased the nutrient loss by run-off from soils by 43% in anionic form and 32% in cationic form. Amount of cation loss were ordered $K^+$ > $Ca^{2+}$ > $Na^+$ > $Mg^{2+}$ > $NH_4{^+} $ and that of anion loss were ordered $SO_4{^{2-}}$ > $NO_3{^-}$ > $Cl^-$ > $PO_4{^{3-}}$. Nutrient loss of sand loam soil in the cation and anion by percolation water increased 1.7 times compared with loam soil. $NO_3{^-}-N$ contents in percolated water were high at the initial stage after compost application, and the amounts were higher in sandy loam soil than loam soil. The maize cultivation also decreased the $NO_3{^-}-N$ contents in percolated water by 82% in loam soil, and 58% in sand loam soil. Soil pH of composts determined by laboratory incubation test increased pH 6.1~6.8 application with poultry and cow manure compost but application with human excrement sludge decreased pH 4.5~4.7. Soil EC were increased initially composts application and decreased up to 2 weeks, thereafter kept a certain level. Nitrogen mineralization rates of composts determined by laboratory incubation test at $25^{\circ}C$ were 39~76% in sandy loam soil, and 16~48% in clay loam soil.
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