• Journal of Microbiology
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• 제40권3호
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• pp.183-192
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• 2002

Cells infected With equine herpesvirus type-1 (EHV-1) Produced both infectious and non-infectious Virus-related particles. Compared to the whole virion, non-infectious particles termed L-particles were deter-mined to lack 150 kDa protein, commonly known as nucleocapsid protein. The potential of L-particles to induce immune responses was studied in mice and foals. Intranasal immunization with L-particles or whole virions induced poor IgG antibody responses in mice. Interestingly, despite the poor antibody response, the conferred immunity protected the host from challenge infections. This was indicated by a significant reduction in virus titers in line with recovery towards normal body weight. Subsequently, the test on the usefulness of L-particles as immunizing agents was extended to foals. Immunization of specific-pathogen-free (SPF) foals resulted in similar results. As determined by a complement-fixing-antibody test (CFT), foals seroconverted when they were immunized either with inactivated L-particles or whole virions via intramuscular (i.m.) injections. The presence of the antibody correlated with the degree of protection. Beyond day 1 post challenge infection (p.i.), there was no virus shedding in the nasal mucus of foals immunized with whole EHV-1 virions. Virus shedding was observed in foals Immunized with L-particles but limited to days 6 to 8 p.i. only. In contrast, extended vim shedding was observed in non-immunized foals and it was well beyond day 14 p.i. Viremia was not detected for more than four days except in non-immunized foals. Immunization in mice via intranasal (i.n.) conferred good protection. However, compared to the i.n. route, a greater degree of protection was obtained in foals following immunization via i.m. route. Despite variation in the degree of protection due to different routes of immunization in the two animal species, our results have established significant evidence that immunization with L-particles confers protection in the natural host. It is suggested that non-infectious L-particles should be used as immunizing agents for vaccination of horses against EHV-1 infection.

• 대한수의학회지
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• 제43권3호
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• pp.471-476
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• 2003

Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by ovine herpesvirus 2 (OvHV-2). OvHV-2 is a gamma herpesvirus, which induces frequent latent infection and often difficult to detect its antigens and even specific nucleic acids because of its low viral copies in the infected tissues. Histopathology, serology and polymerase chain reaction (PCR) were compared for the diagnosis of MCF using 10 bison infected with OvHV-2. Histopathological diagnosis was performed using the criteria which was based upon the pathognomic lesions. Serological diagnosis was conducted using its serum with competitive ELISA for the detection of antibodies of OvHV-2. Also, the nest PCR was performed with peripheral blood leukocytes for the detection of OvHV-2-specific DNAs. Primers 556 and 775 were used for the primary amplification, and primers 556 and 555 were used for the secondary amplification. As the results, positive cases were 6 by histopahology, 9 by serology and 10 by PCR. As comparing with other diagnostic methods, PCR was found to be more sensitive than histopathology and serology. The recent development of molecular diagnostic assays has provided powerful tools for investigating how viruses survive in nature. Development of PCR specific for viruses has dramatically improved the accuracy of diagnosis of viruses in clinically infected animals. Furthermore, amplification of viral genomic material by nest PCR represents the most sensitive method for the detection of viruses and might be detected successfully even though very low viral DNA copies. So, it could be used as the first choice for the detection of viral DNAs with low copies such as the status of latent infection. However, it has also some limitation of application like as false negative results by PCR inhibitors and false positive results by contamination. The results of this study suggest that the use of molecular biological methods like PCR may increase the accuracy for the diagnosis of infectious diseases. However, in diagnostic laboratory, it is recommended that PCR assay must be conducted with other diagnostic methods for more reliable diagnosis.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.112-112
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• 1993

바이러스 치료제 개발을 위하여 합성된 핵산유도체 11개에 대한 in vitro 항바이러스 약효검색을 수행하였다. 검색대상 바이러스로서 외피보유 DNA 바이러스인 human herpesvirus에 속하는 herpes simplex virus type 1과 type 2에 대해서는 Vero 세포체계에서 3일 후 CPE 저해정도를 MTT 검색법으로 cytomegalovirus에 대해서는 HEL 세포체계에서 7일 후 Giemsa 염색법으로 약효를 측정하였다. 외피비보유 RNA 바이러스인 picornavirus에 속하는 poliovirus type 1과 type 3과 coxsackie B virus type 3에 대한 약효를 HeLa 세포체계에서 2일 후 CPE 저해정도를 MTT 검색법으로 측정하였다. 아울러 selectivity index를 구하기 위하여 Vero와 HeLa 세포에 대한 약물자체의 독성인 cytocidal effect를 MIT 검색법으로 측정하였다. 그 결과 항 herpesvirus 약효는 어떤 물질에서도 발견되지 않았으나 한 물질이 poliovirus type 1과 3에 대하여 selectivity index 10정도 (CC$_{60}$ 38 ug/ml, EC$_{50}$ 1-4 ug/ml)를 나타내었고 자세한 기작은 좀 더 조사할 필요가 있다.

• 한국어병학회지
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• 제24권3호
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• pp.237-245
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• 2011

2000~2010년까지 감염성질병 발생에 의한 것으로 의심되는 폐사가 발생한 23개소 양식장의 병든 뱀장어를 대상으로 총 29건의 병원체 검사를 실시하였다. 총 7종의 병원체 감염이 확인되었고, 병원체의 감염률은 Pseudodactylogyrus sp. (65.5%, 19/29 시료), aquabirnavirus (ABV, 62.5%, 15/24 시료), Edwardsiella sp. (44%, 11/25 시료), anguillid herpesvirus 1 (AngHV1, 16.7%, 2/12 시료), Heterosporis sp. (10.3%, 3/29 시료), Vibrio sp. (8%, 2/25 시료), Trichodina sp. (3.4%, 1/29 시료) 순으로 높게 나타났다. 병원체의 감염은 단독감염 (44.8%, 13/29 시료) 보다 혼합감염(62.1%, 18/29 시료) 이 높게 나타났으며, 혼합감염의 경우 2~5종의 병원체가 복합적으로 감염되어 있었다. 혼합감염률은 Pseudodactylogyrus sp.-Edwardsiella sp. (10.3%, 3/29 시료), Pseudodactylogyrus sp.-ABV (10.3%, 3/29 시료), Edwardsiella sp.-ABV (6.9%, 2/29 시료) 순으로 높게 나타났다. 기생충, 세균 및 바이러스에 의한 감염은 조사된 대부분의 지역에서 나타났으며, 수온 $25{\sim}30^{\circ}C$ 범위에 사육되고 있는 뱀장어 치어를 비롯한 성어에 이르기까지 무차별적으로 발생하는 것으로 확인되었다.

• 대한수의학회지
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• 제63권1호
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• pp.4.1-4.5
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• 2023

The prevalence of some swine reproductive and zoonotic diseases in Colombia is unknown, making their management difficult. This study assessed the prevalence of porcine circovirus type 3 (PCV3), Leptospira interrogans, pseudorabies virus, and porcine gamma-herpesvirus by polymerase chain reaction in sows in the productive stage, from farms with a history of reproductive failures, at the department of Tolima. The prevalence of PCV3 was 2.6% and 12.6% for L. interrogans, with some samples co-infected with PCV2. Owing to the coinfections with PCV2, it is necessary to establish whether the interactions between these pathogens were related to the presence of reproductive problems.

• 한국임상수의학회지
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• 제28권4호
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• pp.369-374
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• 2011

Coital exanthema of Thoroughbred horses had occurred firstly in Republic of Korea. This study was performed to evaluate clinical features of coital exanthema, and provide veterinary knowledge to equine practitioner as well as horse farms. The method was clinical, clinical pathology and sexual hormone, electron microscope and libido examination. Five stallions of 27, 45 broodmares of 416 showed clinical symptoms but 124 foals and a teaser did not show any abnormal appearance. Five stallion had papule, pustule, crust around a glans, body of penis, prepuce and remain decolorized spot after treatment. One laceration of glans and 3 gait disorder had been observed but it did not affect libido. Forty-five broodmares localized had papule, pustule, crust, and ulceration around perineal region, vulva, mucocutaneous junction remain decolorized spot after treatment.

• The Korean Journal of Pain
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• 제36권1호
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• pp.4-10
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• 2023

Herpes zoster (HZ) is a common disease in the aging population and immunocompromised individuals, with a lifetime risk of 20%-30% that increases with age. HZ is caused by reactivation of the varicella-zoster virus (VZV), which remains latent in the spinal dorsal root ganglia and cranial sensory ganglia after resolution of the primary VZV infection. The main focus of HZ management is rapid recovery from VZV infection as well as the reduction and prevention of zoster-associated pain (ZAP) and postherpetic neuralgia (PHN). The use of antivirals against VZV is essential in the treatment of HZ. However, limited antivirals are only licensed clinically for the treatment of HZ, including acyclovir, valacyclovir, famciclovir, brivudine, and amenamevir. Fortunately, some new antivirals against different types of Herpesviridae have been investigated and suggested as novel drugs against VZV. Therefore, this review focuses on discussing the difference in efficacy and safety in the currently licensed antivirals for the treatment of HZ, the applicability of future novel antivirals against VZV, and the preventive or therapeutic effects of these antivirals on ZAP or PHN.

• 한국임상수의학회지
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• 제30권3호
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• pp.210-213
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• 2013

고양이 헤르페스 바이러스-1 감염과 관련된 고양이의 괴사성 안면 피부염의 증례를 보고하고자 한다. 3살된, 미거세 수컷 도메스틱 숏헤어 고양이가 2년간 지속된 소양성 피부염을 주증으로 내원하였다. 환자는 생후 약 2개월령부터 지속적인 눈꼽과 결막염을 가지고 있었으며, 눈 주위부터 안면 전체로 확대된 피부염의 병력을 가지고 있었다. 내원 당시에는 가피로 덮여있는 미란과 궤양성의 피부병변이 안면과 회음부에서 주로 관찰되었다. 피부 병변부위의 조직학적 검사 결과, 경계면 피부염을 동반한 기저층의 수포변성과 각질세포의 단세포 괴사 소견이 관찰되었으며, 호산구 침윤을 동반한 표피와 진피의 괴사와 각질세포의 핵내 봉입체도 관찰되었다. 이에 가피 부위의 가검물을 이용하여 PCR검사를 수행하였고, 그 결과 고양이 헤르페스 바이러스-1의 유전자가 검출되었다. 위의 소견으로부터, 본 증례는 고양이 헤르페스 바이러스-1 감염과 관련된 궤양성 피부염으로 진단되었고, 아시클로버의 경구투여와 고양이 인터페론 오메가의 국소투여로 피부 및 점막 병변의 뚜렷한 개선을 볼 수 있었다.

• 한국동물위생학회지
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• 제36권3호
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• pp.187-192
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• 2013

For the monitoring of six viral disease (CIV: canine influenzavirus, CPIV: canine parainfluenzavirus, CHV: canine herpesvirus, CPV2: canine parvovirus type 2, CCoV: canine coronavirus, CNV: canine norovirus) inspections, a total of 300 samples were collected nasal or feces from the companion dogs of animal hospital (n=98) and the abandoned dogs of animal shelters (n=202) in Gwangju, Korea. Using PCR and RT-PCR, CPV2, CPIV and CHV were detected in 55 (18.3%), 11 (3.7%), 1 (0.3%), respectively. CPV2 was highly detected in May, October and November. and CPIV was highly detected in November. But those agents were not detected the virus in March and July. Based on the results of the investigation continuous monitoring for companion and abandoned dogs will be required.

• 한국어병학회지
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• 제23권1호
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• pp.85-98
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• 2010

농림수산식품부와 국립수산과학원에서는 2009년도 방류 품종 (해면 품종 22종과 내수면 품종 11품종)을 대상으로 수산동물전염병의 감염 여부를 검사하였다. 총 12개 지방자치단체에 서 방류를 실시한 것으로 나타났으며, 이 중에서 경상남도, 전라남도, 제주도 및 충청남도는 해면 품종을 주로 방류하였으며, 경기도, 전라북도 및 충청북도는 내수면 품종을 많이 방류하는 것으로 나타났다. 검사품종중에서 해산품종으로는 전복이 24.5%로 가장 많았으며, 그 다음 해삼(15.2%), 넙치 (11.5%), 감성돔과 조피볼락(6.8%), 꽃게 (5.6%), 돌돔 (5.1%), 볼락 (4.6%), 붉은쏨뱅이 (4.5%)로 나타났다. 내수면 품종 중에서는 붕어가 19.4%로 가장 많았으며, 그 다음으로 뱀장어 (17.0%), 동자개 (12.3%), 다슬기 (12.0%), 메기 (8.4)의 순으로 검사 실적이 많았다. 총 33종의 품종을 대상으로 1,080회의 검사가 의뢰되었으며, 검사항목별로 2,066건의 검사를 실시한 결과 19건에서 red sea bream iridovirus (RSIV), koi herpesvirus (KHV) 또는 white spot syndrome virus (WSSV)와 같은 병원체가 검출되어 불합격 처리되었다.