• Journal of Nutrition and Health
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• v.48 no.2
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• pp.157-166
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• 2015

Purpose: The effects of water extract and distillate from the mixture of black goat meat and medicinal herb on MG-63 osteoblast proliferation and mouse bone marrow derived osteoclast formation were investigated. Methods: Proximate composition, volatile basic nitrogen (VBN), mineral content, free amino acid composition and free fatty acid composition in black goat meat were determined. Water extract and distillate were prepared with three groups; goat meat only (BG-E, BG-D), six herbs added group (BG-E6, BG-D6), and eight herbs added group (BG-E8, BG-D8). Osteoblast proliferation, mineralization and calcium uptake activity of MG-63 cells were measured and tartrate resistant acid phosphatase activity of osteoclasts was analyzed. Results: Black goat meat had remarkably low fat and high level of calcium. Glutamic acid was the most abundant amino acid. Herbs added extract groups (BG-E6 and BG-E8) showed increased MG-63 cell proliferation in a concentration dependent manner, while all the distillates did not show the effect. All extracts and distillates showed significantly increased osteoblast mineralization depending on the concentration. In particular, herb added extract, BG-E6, increased 170.3% of control and the distillate of BG-D and BG-D6 increased up to 168.5% and 159.8%, respectively. Calcium uptake activities of all water extracts showed remarkable increase of BG-E6 and BG-E8 up to 615.5% and 628.1% of control, respectively. Ditillates had no effect except BG-D6. All water extracts significantly reduced the activity of tartrate-resistant acid phosphatase (TRAP) in osteoclasts derived from mouse bone marrow. Conclusion: Combination of black goat meat and medicinal herb increased the MG-63 cell proliferation and effectively inhibited osteoclast differentiation in both water extracts and distillate of them, which implies that they could be used as potent functional food materials for bone health.

• Journal of Ginseng Research
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• v.23 no.1 s.53
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• pp.21-37
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• 1999

For the purpose of wide application of Korea Ginseng Radix (KGR) as a tonic and medicinal drug, bibliographical study on Ginseng Radix (GR) was done about its herbal and pharmacological effects known so far. Herbal effect of GR was studied in 28 classical books including Shennongbencaojing(신농본초경), the oldest herbal book in oriental medicine and also its pharmacological effects was compared with its herbal effect. In bibliographical study 38 kinds of efficacies of GR were referred in those books, for example, in fields of gastrointestinal tract, heart, psychology, body fluid, hormone and respiratory tract in order from statistical analysis. GR was chiefly used for supplementing Qi, vital energy in oriental medicine. However, experimental study on respiratory diseases was not reported except lung cancer yet, while GR was used for the treatment of asthma mostly mixed with other herb in oriental medicine. So far research with GR was performed chiefly for isolation of constituents as saponins and evaluation of its efficacy experimentally by KGTR (Korea Ginseng Tobacco Research Institute). From the above studies, to make KGR used widely than before, I suggest study with KGR should be done according to oriental principles such as Qi and tasters and combination theory between herbs excepting typical analytical research with saponins.

• Toxicological Research
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• v.17
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• pp.257-261
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• 2001

Recently, a traditional medicine called Gouteng-san, which consists of eleven herbs, was reported to be effective in treating vascular dementia with a double-blind, placebo-controlled study. Gout-eng-san is also used for patients with vascular dementia in combination with Si-Wu-Tang. The effect of Gouteng-san and Si-Wu-Tang on deficit of learning behavior was investigated using step-down passive avoidance task in mice. Hot-water extract of Gouteng-san (1.5 and 6 g/kg, p.o.) significantly prolonged the step-down latency shortened by scopolamine. The extract of Uncaria hook (150 mg/kg, p.o.), one of the component herb of Gouteng-san, significantly prevented the decrease in the latency after scopolamine. Hot-water extract of Si-Wu-Tang (1.5 and 6 g/kg of dried herbs, p.o.) prevented dose-dependently scopola-mine-induced disruption qf learning behavior. Si-Wu-Tang also prevented the ischemia-induced deficit of learning behavior. Both hot water extract of peony and angelica (1.5 g/kg, p.o.), which are component herbs qf Si-Wu-Tang, prevented the scopolamine-induced learning behavior deficit. Scopolamine (10 uM) suppressed long-term potentiation (LTP) of population spike in the CA1 region of the rat hippocampal slices. Peoniflorin (0.1~ 1uM) extracted from paeony root significantly ameliorated scopolamine-induced inhibition of LTR These results suggest that improvement of deficit of learning behavior by Gouteng-san and Si-Wu-Tang is mediated by direct and/or indirect activation of the cholinergic system in the brain.

• Journal of Pharmaceutical Investigation
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• v.26 no.3
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• pp.215-219
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• 1996

Active component was sought among three herb medicines, which are used in combination as a traditional medicine prescribed for patients with hyperlipidemia related diseases. Antihyperlipidemic effect of this remedy has previously been shown model by the authors on the animal model. Hyperlipidemia was induced on male Wistar rats by keeping them on high lipid diet for one week, as previously described by the authors. Blood lipid profile was verified on these rats by measuring total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL). Then, the diet was changed to normal. At the same time, methanol extracts of Scutellaris baicalensis Georg.(radix), Coptidis japonica Makino(rhizoma) or Rhei koreanum Nakai(rhizoma) were given on daily basis, and changes in the blood lipid profile were monitored for 4 weeks. Methanol extract of Scutellaris baicalensis Georg. significantly reduced the TC value, implying the in vivo antihyperlipidemic effect.

• Journal of Korean society of Dental Hygiene
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• v.13 no.3
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• pp.517-523
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• 2013

Objectives : The aim of the study is to investigate the antimicrobial activity of jaborandi and sorbitol to various oral microbes. Methods : Jaborandi leaves contain pilocarpine. The conditions for extraction were optimized on the basis of substances, temperatures and pHs. Total pilocarpine content after extraction was analyzed by HPLC. The herbal antimicrobial activity of jaborandi and sorbitol were evaluated for oral microbes containing ATCC 25175 S. mutans, ATCC 13419 S. salivarius, ATCC 6249 S. mitis, ATCC 33398 S. equi, ATCC 29213 S. aureus, ATCC 14053 C. albicans. Results : The optimum conditions for highest yielding extraction were pilocarpine content after boiling at $100^{\circ}C$ for 1 hour at pH 3. The level of total pilocarpine content was analyzed at 833 mg/kg by HPLC. The most effective antimicrobial activity was obtained by combination of pilocarpine and sorbitol rather than pilocarpine, menthol and sorbitol, respectively. Conclusions : This results supported the preventive oral health care using safe and convenient jaborandi herb.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.953-958
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• 2013

Selaginella tamariscina is a traditional herb used in medicine. Phytochemical amentoflavone, a biflavonoid class of flavonoids, was isolated from the plant of Selaginella tamariscina. In this study, the antibacterial effects and combination effects of amentoflavone and conventional antibiotics such as ampicillin, cefotaxime, and chloramphenicol were investigated. These results showed that amentoflavone had a considerable antibacterial effect and synergistic interaction with antibiotics against various bacterial strains (fractional inhibitory concentration index ${\leq}$ 0.5), except for Streptococcus mutans. To study the mechanism(s) involved in the synergistic activities between amentoflavone and antibiotics, we detected hydroxyl radical formation using 3'-(p-hydroxyphenyl) fluorescein and measured the $NAD^+/NADH$ ratio by $NAD^+$ cycling assay. The results indicated that the formation of hydroxyl radical would be a cause of the synergistic effect and that this oxidative stress originated from a transient NADH depletion. This study suggests that amentoflavone synergizes with antibiotics and has potential as a therapeutic agent for antimicrobial chemotherapy.

• The Journal of Korean Obstetrics and Gynecology
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• v.30 no.3
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• pp.136-145
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• 2017

Objectives: The aim of this study was to report a case of 43 year-old patient, diagnosed with missed abortion who previously failed to do spontaneous gestational sac expulsion in spite of 2 week-expectant-method, eventually succeeded with Traditional Korean Herb Medicine (TKHM) treatment only. Methods: In transabdominal sonography, gestational sac was not present at week 8. Due to the possibility of effect of medication on future pregnancy and post-surgery complications, D&C (Dilation & Curettage) as well as any other treatment was not done. Instead, she presented to our clinic. A combination of 2 main TKHMs was prescribed with 120-150 cc/dose (2 times per day for a week) : Gunggi-tang for helping expulsion of the dead fetus and placenta, and Wooseul-tang for treating retained placenta. Results: On day 6 of TKHM treatment, initiation of placenta expulsion was done. From day 8, hemorrhage started, lasting for 6 days. On day 10, placenta was expelled. On day 13, gestational sac and placenta were completely expelled. No infection, delayed bleeding, or pain was observed. After bleeding stopped, menorrhea started from week 7. Conclusions: This report implies a potential of TKHM as an optional and safe medication for the natural expulsion of conceptus in missed abortion.

• Journal of Acupuncture Research
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• v.25 no.3
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• pp.19-28
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• 2008

Objectives : The purpose of this study is to investigate the therapeutic effects of oriental medical treatment and oriental-western medical treatment on Bell's palsy. Methods : From 1st June, 2006 to 31th August, 2007, 30 Bell's palsy patients who visited the Facial palsy center at East-West Neo Medical Center within 7days of onset and treated for more than 4weeks were randomly divided into two groups. Group A was treated with oriental medical therapy(Acupuncture, Herb, Physical therapy) and Group B with both oriental and western medical therapy(Acupuncture, Herb, Physical therapy, Administration of prednisone). We evaluated the treatment effect of each group with the House-Brackmann Gross Grading System before treatment, after 1week, 2weeks, 3weeks and 4weeks of treatment. Results : After 1week of treatment, Group A showed better performance compared with Group B. After 3weeks and 4 weeks of treatment, Group B showed better performance than Group A. Conclusions : These results proved that combination treatment of oriental and western medicine was more effective than just oriental medical treatment in long term treatment of Bell's palsy. But we discovered that there were no significant differences between the two groups.

• Food Science and Preservation
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• v.18 no.1
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• pp.87-90
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• 2011

The methanol extracts of 19 commercial herb medicines was analyzed to antibacterial activities against Edwardsiella tarda, causing several fish diseases. Rhus javanica showed most strong antibacterial activity against E. tarda and Escherichia coli. Methanol extract of R. javanica was further extracted using several organic solvents having different polarity. Extract from ethyl acetate fraction showed strong activity against E. tarda as well as E. coli. Minimal inhibitory concentration, MIC of R. javanica extract was measured and resulted showing $64\;{\mu}g/m{\ell}$ for E. tarda and $256\;{\mu}g/m{\ell}$ for E. coli. It is needed that, from these results, further purification and isolation of reposible compound of these activities and further study on the synergy effect using combination with antibiotics against pathogenic bacteria.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.2
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• pp.102-116
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• 2020

The purpose of this study was to analyze the research trends of randomized controlled trials on herbal medicine treatment for atopic dermatitis in China for the last 5 years. We searched for randomized controlled trials with the intervention of herbal medicine for the treatment of atopic dermatitis in the CNKI (China National Knowledge Infrastructure) from January 2014 to December 2018. For the screening of the paper, we used '特应性皮炎' and '异位性皮炎' which mean atopic dermatitis and search was limited to three areas within Medicine & Public Health: Traditional Chinese Medicine, Traditional Chinese Medicinal Herbs, Combination of Traditional Chinese Medicine with Western Medicine. Among the 136 searched studies, we selected a total of 34 studies and analyzed a year of publication, subject characteristics, study design and intervention, prescribed herbal medicine and herbs, pattern identification, evaluation criteria, and outcomes. Longmu decoction (龍牡湯) and Polia Sclerotium (茯笭) was the most frequently prescribed medicine and herb. The most commonly used pattern identification was Blood deficiency and Wind-dryness (血虛風燥), and among them, the most frequently prescribed herb is Rehmanniae Radix (生地黃). In most studies using the total effectiveness and SCORAD index as an outcome measure, the herbal medicine treatment group showed statistically better results than the control group. As a result of the safety assessment, the herbal medicine treatment group was reported having significantly fewer side effects compared to the control group. Hence, it was confirmed that the intervention including herbal medicine had a significant effect on atopic dermatitis. This study would be able to provide the basis of clinical research on atopic dermatitis and applied to the treatment of atopic dermatitis.