• Journal of Environmental Health Sciences
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• v.33 no.4
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• pp.306-316
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• 2007

This study have a object to found out the effects of oriental herb medicine, Antaeeum, to dams of rats and their offsprings. The Antaeeum was savaged to female Sprague-Dawley rats at a dose of 5 mg/kg/day for 3 weeks during gestation periods. Dams of rat were sacrificed at 20th day of gestation, and were observed major internal and reproductive organs. Approximately live fetuses in the 20th days of gestation were selected randomly and examined with stereo microscopes. Others offsprings were fixed with 95% ethanol for skeletal examinations. The fixed fetuses were stained with alcian blue and alizarin red S to observe skeletal variations or malformations. Maternal body weight of Antaeeum treated dams have a tendency of increasing compared with control dams. There were no significant difference in internal and reproductive organs of weight or findings. The spleenic organ relative weight of treated dams were decreased compared with the control significaltly (p<0.05). There were no significant changes between two groups in blood chemistry and hematological values. There were no significant changes in number of corpus luteum, implantation, live fetuses and implantation rate, delivery rate, late resorption rate and sex ratio. But in the Antaeeum treated group showed lower early resorption rate than that of the control dams. Fetal body weight and number of fetus a dam at Antaeeum treated group were higher than that of control group. The fetuses of dams treated with Antaeeum didn't induced external malformations. Vertebral and sternal variations were observed in Antaeeum group, but compared with the control, those variations were not significant. The ossification numbers of rib, cervical, thoracic, and lumber were normal. Fetuses treated with Antaeeum to the dams showed no significant difference in the number of caudal vertebra (P>0.01). From these results, it can be concluded that Antaeeum showed no toxicity effects on maternal side especially on body weight, early resorption rate, and number of live fetuses. Also there were no significant changes on maternal organ weights except spleen, hematological data, reproductive organs. Although skeletal variations were examined at vertebra and sternum, this Antaeeum could not induced significant choses in bone malformation.

• Journal of Applied Biological Chemistry
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• v.52 no.1
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• pp.8-14
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• 2009

This study was conducted to assess the effects of diet food containing Jerusalem artichoke's inulin, lotus leaf, and herb on weight and body fat. Participants in this study were selected based on the following criteria: BMI over $25kg/m^2$, body fat percentage higher than 25%, abdominal obesity level of 0.85 measured by body composition measurement unit (ZEUS 9.9 PLUS, Korea) total 24 female and male university students over 20 of age were assigned to two different groups: control group and diet group and the study was carried out for 30 days. When we measured what the tester's body weight and height, we used an automatic measure machin which is called IMI-1000 from Immanuel company and when we measured what the hip and wist circumference, we used a tape measure. When we measured body mss index (BMI), body Int, body mass, lean mass, waist/hip ratio (WHB), obesity index, we used a ZEUS9.9 PLUS-Korea which is based on bioelectrical impedance analysis, The food intake was checked by means of diet record method to be input into CAN program in order to analyze nutrient intake. Our findings indicated that the diet group, as compared to the control group, lost weight of approximately 2.5 kg and showed statistically significant difference. In addition the level of body Int, muscle, abdominal obesity, obesity, waist and bottom measurement all showed significant decrease after study period. However, there was no big difference in body fat percentage because both body fat level and muscle level dropped together, Putting all these together, diet food in this study containing Jerusalem artichoke's inulin, lotus leaf powder, and herbs powder including nettle, eucalyptus was found to be effective in significant reduction of weight and body fat and obesity-related body indicators. Also, it is considered that this diet food has potential to prevent and improve effectively obesity from abnormal fat accumulation.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.343-352
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• 2010

In order to understand the plant responses to salt stress (0, 50, and 100 mM NaCl), Chinese cabbage seedlings grown up to two leaf stages by hydroponic culture were used. Fresh and dry weight, chlorophyll (Chl), antioxidant materials, polyamine content, antioxidant enzyme activities, and inorganic ion level were evaluated. Fresh and dry weights of Chinese cabbage increased with the increase in salinity while the optimal growth occurred at 50 mM NaCl. The Chl a, total Chl, carotenoid content, and Chl a/b ratio increased by the 6 days after treatment with 100 mM NaCI; however, the Chl b content decreased. Glutathione increased in the root of Chinese cabbage for 6 days. Dehydroascorbate increased remarkably by day 6 caused by the salt stress in both leaf and the root. While ascorbate peroxidase increased, the activity of catalase and glutathione reductase decreased gradually in the first leaf for 6 days. The $Na^+$ content increased by 12.5-fold in the 3 days after treatment with 100 mM NaCI in the shoot, whereas the $Ca^{2+}$, $K^+$, and $Mg^{2+}$ content measured in the same treatment decreased by 43 to 57%. Spermidine content decreased as salinity increased, but spermine content increased. The growth promotion, glutathione and ascorbic acid content in Chinese cabbage were increased by low salt stress, and shortening of the cultivation period for growth increase of Chinese cabbage is expected.

• The Korean Journal of Food And Nutrition
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• v.16 no.4
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• pp.340-346
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• 2003

The diet food used in this test was composed of the total 16 kinds, such as refined water, herb extract, pomegranate extract, polydextrose and isomalto-oligosaccharide etc. Edible fiber as a major component in dietary food was contained to 2.60%(w/w) level. But the calorie was 104.57kca1/100g, contrast of control domestic saengshick product was 368.06kca1. As a result, calorie of dietary food sample was very low level. Vitamin and mineral as a minor component were determined 5, 6 kinds, respectively. On the other hand, dietary effect of body weight loss was determined about 14%(w/w) at animal test result used for rats during 4 weeks. The reason of dietary effect could confirmed from change of plasma neutral lipids, total cholesterol and HDL-cholesterol concentration. Increasing ratio of these in the general diet and dietary diet feeding groups during 4 weeks were 10.55, 6.90, 9.82% and 6.11, 3.85, 6.11%, respectively. from these results, decrease reason of body weight could confirmed the relative relationship with plasma lipids and cholesterol concentration. But plasma plasma glucose and insulin concentration have not positive relativity.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.3 no.3
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• pp.189-196
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• 1999

The community structure, phytomass, and primary productivity in Thuja orientalis stands on a limestone area located in Maepo-up, Chungbuk province in Korea were estimated quantitatively. Seven species including a small proportion of Quercus dentata were identified in the tree layer, 26 species including Ulmus macrocarpa in the shrub layer, and 79 species including Carex lnceolata in the herb layer of the Thuja stands. The vertical distribution of the fine root phytomass exhibited a power functional decrease relative to the soil depth. The seasonal changes in the fine root phytomass at a soil depth of 5 cm were closely related to the pecipitation in the study area. The productivity of the stand of stems, branches, leaves, and roots were 10.72, 0.82, 0.45 and 6.46 ton DM. $ha^{-1}$ .$yr^{-1}$, respectively. The Thuja stand had a high foliage(25%) and low rate of production per unit of foliage. The annual turnover rate of the fine roots int he Thuja stand was 6.71 $yr^{-1}$. The net primary production of the overstory including the understory was estimated at 19.48 ton DM.$ha^{-1}$.$yr^{-1}$ including an underground section of 6.46 ton DM.$ha^{-1}$.$yr^{-1}$(33%). The allocation ratio of net production to root was lower in the limestone Thuja communities than at the nearby non-limestone ones, whereas the production efficiency to leaf weight was higher in the limestone communities. These results would seem to indicate that the limited production capacity is due to the calcium toxicity and low availability of iron and phosphorus in a limestone soil with a high pH, calcium, and bicarbonate content with a strategy for survival in a hostile habitat.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.1
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• pp.96-99
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• 2008

Toxoplasma gondiiis a widespread apicomplexan parasite which is able to infect virtually all warm-blooded vertebrates. Twenty-two percent of the U.S. population is infected, but severe disease in adults is mainly limited to immunosuppressed patients. In patients with acquired immunodeficiency syndrome(AIDS), T. gondii causes a life-threatening opportunistic infection, with Toxoplasma encephalitis as its most severe manifestations. T. gondii is also known to cause congenital infection and is among the pathogens with the highest incidence of complications in pregnancies. Despite its clinical importance, only very few therapeutic drugs against T. gondii are available, all of which target the rapidly dividing tachyzoites, leaving the dormant encysted bradyzoite stage unaffected. We searched 15 traditional medicines that have anti-inflammatory effect from dongyibogam and Traditional Chinese medicine. In vitro studies were performed with HeLa cell cultures, with quantification of Toxoplasma growth by a cell proliferation assay. The result of experiment shows the selectivity of Citrus unshiu Markovich is 6.0. This is higher than sulfadiazine (selectivity was 1.63). For in vivo studies, mice were acutely infected intraperitoneally with $10^5$ tachyzoites of the virulent RH strain and then treated per orally for 4 days from 6 hours postinfection. Efficacy was assessed by sequential determination of parasite burdens in peritoneal cavity. In vivo, Citrus unshiu Markoviche inhibited Toxoplasma growth at a concentration of 10㎎/㎏ of body weight per day, the inhibition ratio was estimated to be 64.01%.

• Journal of Bio-Environment Control
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• v.15 no.1
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• pp.84-90
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• 2006

This study was conducted to anticipate nitrate reduction state in tree through measurement of nitrate reductase activity (NRA) and investigate the effect of nitrogen concentrations (100, 200, 400, and 600 $mg\;L^{-1}$) on growth, the nitrogen content of various tissue, and NRA of pear (Pyrus pyrifolia cv. Niitaka) seedlings in sand culture. Nutrient solutions used in this experiment were adjusted to pH 6.5 and fixed the ratio of ammonium and nitrate to 1:3 and trickle-irrigated 3 times a day. Tree height and dry weight of various organs in seedlings were higher in low nitrogen concentration (100 and 200 $mg\;L^{-1}$) than in high nitrogen concentration (400 and 600 $mg\;L^{-1}$). The shoot growth in 600 $mg\;L^{-1}$ was extremely poor by nitrogen over supply. Increasing the nitrogen concentration, the concentration of nitrate-N in leaves and roots were insignificantly changed but that of stems increased. The accumulation of total and reduced nitrogen in all organs with increasing concentrations of nitrogen supply were increased at 30 days after treatment but those of all organs at 60 and 90 days after treatment were highest in 600 $mg\;L^{-1}$, whereas there were no significant changes among other nitrogen concentration. The in vivo (${+NO_3}^-$) NRA of all organs did not relate to nitrogen concentration but the in vivo (${-NO_3}^-$) NRA of leaves except roots increased with increasing the nitrogen concentration. Therefore, the proper nitrogen concentration to promote growth and nitrate reduction of pear tree was 200 $mg\;L^{-1}$.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.151-161
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• 2019

Ashwagandha (Withania sominifera Duanal) is an important medicinal herb with increased demand after discovering its anti-stress and sex stimulating properties that are attributed to the presence of biologically active alkaloid compounds. The aim of this study was to elucidate a proper agro technological package that ensures the optimum growth of Ashwagandha to obtain the finest quality without degrading the pharmacologically active constituents. Mixtures of organic and inorganic fertilizers were combined with direct seeding and transplanted as four different treatments in this study. The fresh and dry weights of the tubers were recorded up to 12 months starting from two months after sowing (MAS) while the shoot height, root length, number of leaves, fresh and dry weights of the shoot and the root with a shoot ratio of up to 6 MAS were determined. The results revealed that the growth of Ashwagandha was not affected significantly by the method of planting, type of fertilizer or their combinations during most of the harvests. However, tubers harvested at 6 MAS had the highest recorded dry tuber weight per plant in all four treatments compared to the early harvests where two direct seeded treatments had the best results. Comparison of the phytochemical compounds showed that direct seeding with organic fertilizer had the highest recorded values for alkaloid and withaferine A contents with a lower percentage of fiber compared to the treatments with inorganic fertilizer. In conclusion, direct seeding with organic fertilizer and tubers harvested at 6 MAS are recommended as the best cultivation conditions and harvesting stage to obtain high quality tubers of Ashwagandha, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.10
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• pp.1586-1592
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• 2005

In order to develop Houenia dulcis with natural food application the effect of Hovenia dulcis fruits powder content the physical, textural and sensory properties of noodles were examined. The analysis of the contents of free amino acids showed that 17 kinds of amino acids were detected and highest in 3$\%$ Hovenia dulcis fruits powder contents. The weight and the volume of cooked noodle were highest in control the more added of Houenia dulcis fruits powder contorts lessen the weight and the volume (p < 0.01, p < 0.001), the water absorption ratio was highest in control group. Hunter color value of noodle showed that L value was 7.62 in control, a and b values were highest in 5$\%$ added group. More added Houenia dulcis fruits powder contents increased a and b values (p < 0.001). Hardness, cohesiveness (p < 0.05), gumminess and brittleness (p < 0.001) were highest in control, springiness was highest in 1$\%$ added group (p < 0.001). Scanning electron micrographs of noodle showed that more added of Houenia dulcis fruits powder contents made the particle uniform, so softer and larger. Sensory properties showed that color of noodle and herb flavor were thicker in more added of Houenia duzcis fruits powder contents (p < 0.001). The taste was highest in 3$\%$ added Houenia dulcis fruits powder contents. Overall acceptability was also highest in 3% added group (p < 0.01).

• The Korea Journal of Herbology
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• v.27 no.6
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• pp.29-35
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• 2012

Objectives : To observe the effect of 4 kinds of Korean medicine and complex extracts on the anti-stress of chronic mild stress Rat model, so as to find out the machanism of its pharmacological effects. Methods : 64 healthy SD Rats were randomly divided into 8 groups. Model Design by chronic stress(CSM), and carried out the forced swimming test. We investigated the body weight, feed and water intake, feed conversion ratio and measured parameters to changes in swimming time, immobility time, 2 and 24 hours of feces, corticosterone hormone and blood glucose level. Results : In this experiments, body weight gain and feed intake were higher in all korean medicinal herb group. Swimming time were increased by Zizyphus jujuba and Gagamsanzointang(SZT) groups and immobility time was significantly lower by Zizyphus jujuba and Fluoxetine(FLX) groups. The result of corticosterone hormone level were significantly lower in Fluoxetine(FLX) and Gagamsanzointang(SZT) but blood glucose was not significantly difference. In 2 hours after fecal frequency result were significantly lower in Zizyphus jujuba group. but SZT group were increased. This result caused by acute stress but 24 hours of feces were restored to normal. Conclusions : 5 kind of Korean Medicinal Herbs extracts has good anti-stress effects, and also to clear the circulatory system and regulate blood glucose and improve bowel function. We suggest that use a single Korean medicine rather than the combination to relieve stress.