• 대한한의학방제학회지
• /
• 제16권2호
• /
• pp.243-254
• /
• 2008

This study is a clinical report for hepatitic C patient and alcoholic hepatitis patient who were improved by treatment of a herbal viscous extracts(Chunggan plus). We checked up Aspartate-aminotransferase(AST), Alamine-aminotransferase(ALT) and ${\gamma}$-Glutamyl transpeptidase (${\gamma}$-GTP) and compared the level of AST, ALT, ${\gamma}$-GTP after treatment. After medication the level of AST, ALT, ${\gamma}$-GTP was significantly normalized. So we suggested that herbal viscous extracts(Chunggan plus) has effects on hepatitis.

• 대한의용생체공학회:학술대회논문집
• /
• 대한의용생체공학회 1991년도 추계학술대회
• /
• pp.57-60
• /
• 1991

A new approach to texture classification for ultrasound liver diagnosis using run difference matrix was developed. The run difference matrix consists of the gray level difference along with distance. From this run difference matrix, we defined several parameters such as LDE, LDEL, NUF, SMO, SMG, SHP etc. and three vectors namely DOD, DGD and DAD. Each parameter value calculated in fatty cirrhotic, chronic hepatitic and normal liver mage was plotted in two dimensional plane. We compared our results with run length method. There are several advantages of run difference matrix method over the run lengths. 1) It is more sensitive to small difference of gray level distribution. 2) The parameters provide more statistically significant value. Images were classified with the extracted parameters to each diseases using neural networks. In preliminary clinical exprements, this approach showed satisfying results.

• Bulletin of the Korean Chemical Society
• /
• 제26권2호
• /
• pp.285-291
• /
• 2005

The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is the viral RNA-dependent RNA polymerase (RdRp), which is the essential catalytic enzyme for the viral replication and is an appealing target for the development of new therapeutic agents against HCV infection. A small amount of serum from a single patient with hepatitis C was used to get the genome of a Korean HCV isolate. Sequence analysis of NS5B 1701 nucleotides showed the genotype of a Korean isolate to be subtype 1b. The soluble recombinant HCV NS5B polymerase lacking the C-terminal 24 amino acids was expressed and purified to homogeneity. With the highly purified NS5B protein, we established in vitro systems for RdRp activity to identify potential polymerase inhibitors. The rhodanine family compounds were found to be potent and specific inhibitors of NS5B from high throughput screening (HTS) assay utilizing the scintillation proximity assay (SPA) system. The binding mode of an inhibitor was analyzed by measuring various kinetic parameters. Lineweaver-Burk plots of the inhibitor suggested it binds not to the active site of NS5B polymerase, but to an allosteric site of the enzyme. The activity of NS5B in in vitro polymerase reactions with homopolymeric RNA requires interaction with multiple substrates that include a template/primer and ribonucleotide triphosphate. Steady-state kinetic parameter, such as Km, was determined for the ribonucleotide triphosphate. One of compounds found interacts directly with the viral polymerase and inhibits RNA synthesis in a manner noncompetitively with respect to UTP. Furthermore, we also investigated the ability of the compound to inhibit NS5B-directed viral RNA replication using the Huh7 cell-based HCV replicon system. The investigation is potentially very useful for the utility of such compounds as anti-hepatitic agents.