• Biomolecules & Therapeutics
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• 제4권1호
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• pp.46-50
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• 1996

Inhibition of the growth of human cancer cells by proteins secreted from 373-L1 cells was investigated in the present study. The growth of human cancer cells was inhibited by co-culture with 373-L1 cells under 10% FBS and DME, DME, GIT and serumless medium, respectively. The conditioned medium of cultured 373-L1 cells under serumless medium was concentrated 100-fold through an ultrafiltration cell with a 10,000 molecular weight cutoff at 4$^{\circ}C$ under positive pressure using nitrogen(373-L1 EM). 373-L1 EM inhibited the growth of HeLa, Hep G 2, KHOS-Np, A43l and MCF-7 cells. 3T3-L1 EM was purified with FPLC, DEAE-ion exchange chromatography and phenyl-sepharose chromatography. The major protein of 373-L1 EM has a molecular weight of 66,000-68,000 in SDS-PAGE analysis. The results suggest that the inhibitory activity of 373-L1 EM appears to be due to some protein(m.w.66,000-68,000) secreted by 373-L1 cells.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.61-66
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• 2016

It has been known that Arak, Salvadora persica, has a number of medicinal properties. We tried to investigate in vitro scolicidal effect of root extracts of this plant against protoscolices from hydatid cysts of Echinococcus granulosus. Protoscolices were aseptically collected from sheep livers containing hydatid cysts. S. persica root extract was used in 10, 30, and 50 mg/ml concentration for 10, 20, and 30 min. The viability of protoscolices was ascertained by 0.1% eosin staining. Scolicidal activity of S. persica extract at a concentration of 10 mg/ml was 36.3%, 50.3%, and 70.8% after 10, 20, and 30 min of exposure, respectively. The scolicidal effect of this extract at a concentration of 30 mg/ml was 52.9%, 86.7%, and 100% after 10, 20, and 30 min of exposure, respectively. S. persica extract at a concentration of 50 mg/ml, meanwhile, killed 81.4%, 100%, and 100% of protoscolices after 10, 20, and 30 min, respectively. Also, the cytotoxic potential of S. persica was assessed on human liver cells (HepG2) using trypan blue exclusion test. No cytotoxic effect was observed on HepG2 cell line. The present study confirmed for the first time that the ethanolic extract of S. persica has high scolicidal power in vitro. However, in vivo effect of this material remains to be studied for treatment of echinococcosis in humans and herbivorous animals.

• 대한융합한의학회지
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• 제5권1호
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• pp.45-54
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• 2023

Objectives : Alcohol-induced liver disease advances as to reactive oxygen species (ROS) and cellular lipid peroxidation increase. We examined the hepatoprotective effects of Zingiber officinale Roscoe rhizome extract (ZR), Pueraria lobata Ohwi flower extracts (PF), and a newly developed herbal juice (HJ), which was a combination of ZR and PF extracts, against ethanol-induced hepatotoxicity. Methods: The study utilized the human hepatoma cell line HepG2 cells to validate the hepatoprotective effect of HJ (50~200 ㎍/mL) against ethanol (EtOH, 700 mM)-induced liver damage. Results: HJ effectively reduced the protein expression of sterol regulatory element-binding transcription factor 1, adiponectin, and AMP-activated protein kinase in EtOH-induced HepG2 cells. The levels of ROS, total cholesterol, and triglycerides, which are the result of various synthesis and lipogenesis processes induced by EtOH in the liver, were reduced by HJ. Furthermore, the activities of alcohol dehydrogenase and aldehyde dehydrogenase, enzymes linked to alcohol degradation, were more effectively downregulated by HJ treatment compared to treatment with ZR and PF alone, all without causing cytotoxic effects. Conclusions: HJ protects the liver by inhibiting EtOH-induced lipogenesis, lowering ROS generation, and improving alcohol degradation, which is more effective than ZR and PF alone. Further, in vivo experiments can offer additional evidence regarding the effectiveness, safety, and underlying mechanism of action of HJ.

• Asian Pacific Journal of Cancer Prevention
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• 제17권11호
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• pp.4929-4934
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• 2016

Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the commercially available chemotherapies, natural products-loaded nano-composites are recommended to improve cancer targetability and decrease the harmful impact on normal cells. This study aimed at exploring the anti-cancer impacts of Moringa oleifera seed oil in its free- (MO) and nano-formulations (MOn) through studying whether it mechanistically promotes mitochondrial apoptosis-mediating cell death. Mitochondrial-based cytotoxicity and flow cytometric-based apoptosis analyses were performed on cancer HepG2, MCF7, HCT 116, and Caco-2 cell lines against normal kidney BHK-21 cell line. The present study resulted that MOn triggered colorectal cancer Caco-2 and HCT 116 cytotoxicity via mitochondrial dysfunction more powerful than its free counterpart (MO). On the other side, MOn and MO remarkably induces HCT 116 mitochondrial apoptosis, while sparing normal BHK-21 cells with minimal cytotoxic effect. The present results concluded that nano-micelle of Moringa oleifera seed oil (MOn) can provide a novel therapeutic approach for colorectal and breast cancers via mitochondrial-mediated apoptosis, while sparing normal and even liver cancer cells a bit healthy or with minimal harmful effect. Intriguingly, MOn induced breast cancer not hepatocellular carcinoma cell death.

• 한국식품저장유통학회지
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• 제9권1호
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• pp.114-119
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• 2002

본 연구에서는 건강기능성 버섯으로 알려진 A. blazei 에 대하여 재배시 농가에서 사용하는 배지의 종류에 따라 생산된 자실체의 면역 기능성 차이를 규명 할 목적으로 in viro 상태에서 추출다당체의 세포에 대한 영향을 살펴보았다. 기능성 약용 버섯인 A. balzei 에 대하여 재배시 농가에서 사용하는 배지에 따른 in viro 상태에서 세포에 대한 다당체의 영향을 검토하였다. 추출용매에 따른 항종양 물질의 저해효과는 약 25∼30% 수율범위의 methanol 추출물이 비교적 높은 효과를 나타내었다. 이 추출물을 이용하여 human colon carcinoma cell line인 HT29와 human heptoma cell line HepG2에 대한 항종양실험 결과 결장암 세포에 대한 효과는 고농도로 처리시 사용된 생산배지에 따라 1.5∼3.5배 까지의 차이를 나타내었다. 간암세포에 대해서도 고농도로 처리시 사용된 생산배지에 따라 1.3∼2.4배 까지의 저해효과를 볼 수 있었으며 이는 배지상의 조성과 발효에 따른 차이로 추측된다. 국내산 신령버섯간 지역별로 다르게 사용되는 생산배지 사이에 나타나는 항종양효과에서 일반적으로 많이 사용되는 볏짚과 사탕수수 혼합배지보다는 볏짚대신에 칡을 사탕수수에 일부 혼합하여 발효시킨 배지에서 생산된 버섯에서 상당히 높은 종양 저해효과를 나타내었다. 즉 결장암에 대한 처리시 저 농도에서는 약 1.5배, 고농도에서 약 1.6배의 저해효과를 나타내었으며, 간암세포에서는 저 농도에서 약 2.1배, 고농도에서 약 2.8배의 더욱 높은 저해효과를 나타내었다. 건조 분말시료에서 지용성 성분을 제거한 후 열수 및 냉·온 알칼리성 조건으로 다당체를 분리하여 Ab-l, Ab-2, Ab-3 분획을 얻었으며, 이들 시료로 Salmonella typhymurium TA100을 이용한 항돌연변이 실험을 수행한 결과 재배지역과 사용배지에 따른 큰 차이는 나타나지 않았지만 대체로 높은 항돌연변이 효과를 나타내었다. 면역세포의 활성정도를 측정하기 위하여 macrophage Raw cell에 상기의 fraction을 적용해본 결과 칡 사용 생산버섯의 알칼리성 추출 Ab-3 fraction이 사용한 다른 배지에 비하여 약 45∼58% NO 생성증가를 나타내었으며, 기타 열수추출 fraction에서는 유의적인 큰 증가는 없었으나 알칼리 추출조건에서는 대체로 증가하는 경향이 뚜렷하게 나타났다.

• Preventive Nutrition and Food Science
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• 제11권3호
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• pp.191-197
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• 2006

Matrix metalloproteinase-2 (MMP-2) is a key enzyme involved in tumor invasiveness. The plant of Magnolia officinalis Rehd. et Wils. is often included as an ingredient in various herbal remedies recommended for cancer theraphies in Korea. Various extracts prepared from stems of M. officinalis were tested for cytotoxic activity on human hepatocellular carcinoma cell line, SK-Hep cells using the XTT assay method. Then, the inhibitory effect was examined on MMP-2 activity using gelatin zymography. Methanol (MeOH) extract of M. officinalis caused the strongest inhibition of the MMP-2 activity, as measured by gelatin zymography method for enzyme activity. $IC_{50}$ values of fractions on MMP-2 activity were in a range of $4.9{\sim}11.3\;{\mu}g/mL$. Among each fraction, butanol and ethylacetate (EtOAc) fractions showed the strong inhibitory activities ($IC_{50}=10.7\;and\;4.9\;{\mu}g/mL$, respectively). When the M. officinalis's constituents such as magnolol, honokiol, (-)-epigallocatechin gallate (EGCG) and ovovatol were examined for inhibitory effects on MMP-2 activity, EGCG showed strong inhibitory activity. However, MeOH extract of M. officinalis was dose-dependently inhibited to MMP-2 activity. The MeOH extract, hexane and EtOAc fractions $(IC_{50}\;of\;>200\;{\mu}g/mL)$ exhibited weak cytotoxicity activity, while butanol $(IC_{50}=80\;{\mu}g/mL)$ and chloroform fractions $(IC_{50}=90\;{\mu}g/mL)$ exhibited relatively strong cytotoxic activity. From these results, M. officinalis could be suitable for cancer treatment and chemopreventive drugs.

• 한국미생물·생명공학회지
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• 제30권1호
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• pp.51-56
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• 2002

국내 토양에서 분리한 식물성 병원균인 Alternaria brassicicola SW-3리 항암활성 물질 생산능을 조사하고, 활성물질을 분리 정제하여 구조를 확인하였다. A. brassicicola SW-3는potato dextrose broth를 이용하여 15$^{\circ}C$에서 2주간 진탕 배양한 다음, MTT assay를 실시하여 항암활성을 확인하였으며, 배양여액 중의 항암물질은 ethyl acetate로 추출하고, silica gel column chromatography로 정제하여 무색의 oily product를 얻었다(수율 22mg/m1). 분리된 물질은 물이나 hexane에는 녹지 않고, chloroform, ethyl acetate, ethanol 등에는 잘 녹는 특징을 보였으며, , $IR^{1}$H-NMR, $^{13}$C-NMR 등을 통해 구조를 분석한 결과, 최근에 일본에서 분리되어 항암효과가 있는 것으로 알려진 depudecin과 동일한 물질로 추정되었다. 본 실험에서 분리된 depudecin은 인체간암세포와 mouse 피부암세포에 대한 세포독성을 나타내었으며, 각각의$ IC_50$ 는 $57\mu$g/ml, $69\mu$g/ml로 나타났다. Alternaria brassicicola SW-3에 의해 생산된 물질이 기지의 물질이지만, depudecin은 아직 작용 기작이나 적용범위 등이 밝혀지지 않은 초기 연구 단계에 있는 물질로서, 새로운 항암제로서의 가능성이 매우 높아 이의 유도체를 합성하거나, 다른 항암제와의 혼용에 의해 부작용이 적은 강력한 항암제를 개발하기 위한 선도물질로 활용될 수 있을 것이다.

• 통합자연과학논문집
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• 제7권4호
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• pp.241-252
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• 2014

A series of N-benzoylated ligands incorporating three different benzoyl groups 2,2'-(benzoyliminodiethylene)-4-substituted phenols ($L^{1,4,7}$), 2,2'-(4-nitrobenzoyliminodiethylene)-4-substituted phenols ($L^{2,5,8}$) and 2,2'-(3,5-dinitrobenzoyliminodiethylene)-4-substituted phenols ($L^{3,6,9}$) were synthesized and characterized by IR, $^1H$ NMR, $^{13}C$ NMR and mass spectroscopy. The In vitro antibacterial activity of investigated ligands were tested against human pathogenic bacteria such as four Gram (-) Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio cholera, Vibrio harveyi and two Gram (+) Staphylococcus aureus, Streptococcus mutans. Furthermore, docking studies were undertaken to gain insight into the possible binding mode of these compounds with the binding site of the topoisomerase II (PDB: 4FM9) enzyme which is involved in DNA superhelicity and chromosome seggregation. The N-benzoylated derivatives $L^{5,7,8}$ have significant anticancer activity as Topoisomerase inhibitors. The ligands $L^5$ and $L^8$ were tested for their anticancer activity against human liver adenocarcinoma (HepG2) cell line with the MTT assay.

• 한국작물학회지
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• 제57권4호
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• pp.353-358
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• 2012

본 연구에서는 검정찰옥수수 종실을 일반분쇄와 저온미세분쇄를 하여 입자크기에 따른 항산화 활성과 cytotoxicity를 평가하여 이용성 증진을 하고자 연구를 수행하였다. 본 연구의 결과를 요약하면 다음과 같다. 1. 분쇄 정도에 따른 평균값은 일반분쇄가 $473.7{\mu}m$, 저온미세분쇄 $17.2{\mu}m$이었으며 중간값은 $336.9{\mu}m$와 $13.4{\mu}m$를 나타내었다. 2. TEAC 활성 측정 결과 일반분쇄는 $3.87{\mu}mol$ TE/g로서 저온미세분쇄 $5.15{\mu}mol$ TE/g보다 낮은 활성을 보였다. FRAP 활성 측정에서는 저온미세분쇄가 $10.08{\mu}mol$ Fe(II)/g로 일반분쇄 $8.86{\mu}mol$ Fe(II)/g보다 높은 활성을 보였다 3. 간암 세포주(Hep-G2) 성장억제에 미치는 영향은 1 mg/ml 농도에서 검정찰옥수수 종실을 일반분쇄(31.48%)한 것보다 저온미세분쇄(27.41%)를 한 경우가 암세포 생장 억제 효과를 큰 것으로 나타났다. 4. 대장암 세포주인 HCT-116에서는 1 mg/ml에서 분쇄정도에 따라 큰 차이를 보이지 않았으며, 유방암 세포주(MCF-7)에서는 일반분쇄가 미세분쇄보다 높은 생장억제를 보였다.

• Molecules and Cells
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• 제45권12호
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• pp.935-949
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• 2022

Liver cancer has a high prevalence, with majority of the cases presenting as hepatocellular carcinoma (HCC). The prognosis of metastatic HCC has hardly improved over the past decade, highlighting the necessity for liver cancer research. Studies have reported the ability of the KiSS1 gene to inhibit the growth or metastasis of liver cancer, but contradictory research results are also emerging. We, therefore, sought to investigate the effects of KiSS1 on growth and migration in human HCC cells. HepG2 human HCC cells were infected with lentivirus particles containing KiSS1. The overexpression of KiSS1 resulted in an increased proliferation rate of HCC cells. Quantitative polymerase chain reaction and immunoblotting revealed increased Akt activity, and downregulation of the G1/S phase cell cycle inhibitors. A significant increase in tumor spheroid formation with upregulation of β-catenin and CD133 was also observed. KiSS1 overexpression promoted the migratory, invasive ability, and metastatic capacity of the hepatocarcinoma cell line, and these effects were associated with changes in the expressions of epithelial mesenchymal transition (EMT)- related genes such as E-cadherin, N-cadherin, and slug. KiSS1 overexpression also resulted in dramatically increased tumor growth in the xenograft mouse model, and upregulation of proliferating cell nuclear antigen (PCNA) and Ki-67 in the HCC tumors. Furthermore, KiSS1 increased the angiogenic capacity by upregulation of the vascular endothelial growth factor A (VEGF-A) and CD31. Based on these observations, we infer that KiSS1 not only induces HCC proliferation, but also increases the metastatic potential by increasing the migratory ability and angiogenic capacity.