• BMB Reports
• /
• 제40권1호
• /
• pp.113-118
• /
• 2007

Tolaasin, a pore-forming peptide toxin, is produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. P. tolaasii 6264 was isolated from the oyster mushroom damaged by the disease in Korean. In order to isolate tolaasin molecules, the supernatant of bacterial culture was harvested at the stationary phase of growth. Tolaasin was prepared by ammonium sulfate precipitation and three steps of chromatograpies, including a gel permeation and two ion exchange chromatographies. Specific hemolytic activity of tolaasin was increased from 1.7 to 162.0 HU $mg^{-1}$ protein, a 98-fold increase, and the purification yield was 16.3%. Tolaasin preparation obtained at each purification step was analyzed by HPLC and SDS-PAGE. Two major peptides were detected from all chromatographic preparations. Their molecular masses were analyzed by MALDI-TOF mass spectrometry and they were identified as tolaasin I and tolaasin II. These results demonstrate that the method used in this study is simple, time-saving, and successful for the preparation of tolaasin.

• Journal of Applied Biological Chemistry
• /
• 제61권3호
• /
• pp.213-217
• /
• 2018

Pseudomonas tolaasii에 의해 분비되는 톨라신은 펩티드 독소로서, 버섯 자실체 구조와 세포를 파괴하여 갈반병을 일으킨다. 톨라신의 독성은 용혈활성을 측정함으로서 평가하며, 이는 톨라신 분자가 적혈구 막에 pore를 형성하여 세포 구조를 파괴하기 때문이다. 이전 연구에서, $Zn^{2+}$ 뿐만 아니라 $Ni^{2+}$이 톨라신의 세포독성에 억제효과를 가짐을 확인하였다. $Ni^{2+}$은 농도가 증가함에 따라 톨라신에 의한 용혈작용을 저해하였으며, 이의 $K_i$ 값은 1.8 mM이었다. 용혈활성은 10 mM 이상의 농도에서 완전히 저해되었다. $Ni^{2+}$의 효과는 pH에 따라 크게 변하지 않았으나, $Zn^{2+}$의 톨라신 세포독성 억제 효과는 염기성 pH에서 크게 증가하였다. 완충액의 pH를 7에서 9로 증가시키면, 50% 용혈작용이 일어나는 시간인 $T_{50}$은 1 mM $Ni^{2+}$에 의해 조금 증가하였으나 $100{\mu}M$ $Zn^{2+}$에서는 크게 증가하였다. $Zn^{2+}$와 $Ni^{2+}$을 반응용액에 동시에 처리하였을 때, 두 양이온의 상승효과는 모든 pH에서 나타났다. 서로 다른 pH 의존성을 보이는 두 금속이온의 분자적 설명은 톨라신의 pore 형성과 세포 독성에 관한 기작의 이해에 기여할 것이다.

• 환경위생공학
• /
• 제6권1호
• /
• pp.31-46
• /
• 1991

Aeromonas hydrophila which bacause various diseases in human also infects fresh water fish, severly damaging the fishing industries. To prevent disease in humans and reduce damaging on the fishing industries, We have examined several characteristics of Aeromonas hydrophila and obtained the following results. All of the strains gave a posive voges-proskauer, methyl-red, salicin and esculin reaction. Seventeen(94.4%) A. hydrophila strains presented the phenotype SP-PAB- in autoagglut-ination test, but only strain AH 997 showed $SP^{+}PAB^{+}$. in autoagglut ination test, but only strain AH 997 $SP^{+}PAB^{+}$ All of the strains took up the censored to various degrees. Three of 18 strains showed positive reaction in crystal violet binding test. Hemolytic activity ranged from titers of 0 to 1/256. Seven of the 17(38.8%) A. hydrophila strains were positive in sucking mouse assay. Cytotoxin activity on vero and RK cells was displayed various titers.(1/2-1/1024)

• 대한의생명과학회지
• /
• 제12권1호
• /
• pp.35-42
• /
• 2006

Alloimmunization to red blood cell (RBC) antigens may cause a delayed hemolytic transfusion reactions (DHTR) and a delayed serologic transfusion reactions (DSTR). In the present study, the frequency of alloimmunization and its clinical significance were evaluated. Also, transfusions were correlated with antibody formation. Alloimmunization rate was 0.63%. Alloimmunization rate in multiple transfused patients was 24.5%. The most common clinically significant alloantibodies of alloimmunized patients were found to be Rh antibodies (52.6%). Nine patients out of 38 (23.7%) became undetectable after the first detection. To be positive at antibody screening test after RBC transfusion was mean transfused numbers: 3.7 units, mean transfused periods: 56 days, mean transfused frequencies: 1.7 times. The results from antibody specificity and RBC transfusions were comparatively analyzed and it shows that Rh system antibodies were longer than other antibodies (P<0.05). In case of disease group, malignant diseases was longer than other diseases (P<0.05). In order to prevent the formation of RBC alloimmunization, irregular antibody screening tests were performed at propriety intervals in multiple transfused patients.

• 보험의학회지
• /
• 제2권1호
• /
• pp.75-81
• /
• 1985

Urine contains protein and nucleic acid(urea, uric acid, creatinine, ammonia, amino acids), various organic and inorganic materials, vitamin, hormone, enzyme etc. The examination of gualitative or quantitative change of the above mentioned materials and picking up the abnormal materials are useful to diagnose diseases. The test strip for examination of urine is applied to the routine test, monitoring of medical therapy and recurrence, self monitoring, and screeing in preventive medicine. We have been using multitest strip for checking the bacterial infection(nitrite), PH, protein, glucose, ketone body, urobilinogen, bilirubin and occult blood. So it is possible to diagnose three groups of diseases as follows, abnormal metabolism of the carbohydrate, diseases of kidney and other urogenital system, diseases of hepatobiliary system and hemolytic disease causing abnormal metabolism of bile juice.

• Journal of Trauma and Injury
• /
• 제33권2호
• /
• pp.112-118
• /
• 2020

In trauma patients, coagulopathy and abnormal increases or decreases in cell counts are frequently observed, and are associated with high mortality and morbidity in the acute phase of trauma. Because major trauma is often life-threatening, and hematologic abnormalities are multi-factorial and transient, major blood loss is usually suspected to be the primary cause of these abnormalities, and much time and cost may be spent attempting to identify a focus of hemorrhage that might or might not actually exist. Persistent abnormalities in the complete blood count, however, require clinical suspicion of other hematologic diseases to minimize improper transfusions and to improve outcomes, including mortality. Physicians at trauma centers should be familiar with the clinical characteristics of hematologic diseases and should consider these diseases in trauma patients. In this report, we present cases of two hematologic disorders found in trauma patients: autoimmune hemolytic anemia induced by systemic lupus erythematosus and myelodysplastic syndrome.

• Childhood Kidney Diseases
• /
• 제20권1호
• /
• pp.6-10
• /
• 2016

Poststreptococcal glomerulonephritis (PSGN) is one of the most recognized diseases in pediatric nephrology. Typical clinical features include rapid onset of gross hematuria, edema, and hypertension, and cases are typically preceded by an episode of group A ${\beta}$-hemolytic streptococcus pharyngitis or pyoderma. The most common presenting symptoms of PSGN are the classic triad of glomerulonephritis: gross hematuria, edema, and hypertension. However, patients with PSGN sometimes present with unusual or atypical clinical symptoms that often lead to delayed diagnosis or misdiagnosis of the disease and increased morbidity. Additionally, the epidemiology of postinfectious glomerulonephritis (PIGN), including PSGN, has changed over the past few decades. This paper reviews atypical clinical manifestations of PSGN and discusses the changing demographics of PIGN with a focus on PSGN.

• Journal of Yeungnam Medical Science
• /
• 제35권2호
• /
• pp.227-231
• /
• 2018

Left main coronary artery (LMCA) thrombosis is rare and the cause should be determined. A previously healthy young man presented with severe chest pain and dyspnea. The electrocardiogram showed typical ST-segment elevation myocardial infarction with clinical instability. Emergency coronary angiography revealed complete LMCA occlusion by thrombosis. After reperfusion, the patient was admitted to the cardiac care unit. He was diagnosed with hemolytic anemia and tested positive for antinuclear antibodies. Systemic lupus erythematosus (SLE) and LMCA disease due to systemic thrombosis were diagnosed. Steroids were started and the patient was discharged without complications. We report this rare case of LMCA thrombosis as an initial presentation of SLE.

• Childhood Kidney Diseases
• /
• 제18권1호
• /
• pp.42-46
• /
• 2014

가와사끼병은 전신성 혈관염을 일으키는 질환중의 하나로 여러 장기들을 침범할 수 있다. 신장증세로는 농뇨, 혈뇨, 단백뇨, 간질성 신염, 급성 신부전증, 용혈성 요독 증후군, 신반흔 등이 있다. 가와사끼병의 신장침범에 대한 병리기전은 아직 알려져 있지 않지만, 자가면역질환으로 인한 것으로 사려된다. 가와사끼병이 요로감염 이 후에 발병한다는 몇몇 보고들이 있었다. 하지만, 이미 보고된 논문들에 포함된 많은 요로감염 환자들은 신장방광 초음파, DMSA 스캔이나 배뇨중 요도방광조영술 등을 모두 받은 경우는 없었다. 이에 저자들은 급성 신우신염이 재발한 후 불완전 가와사끼병이 발생한 고도의 방광요관역류가 있는 8개월 남아를 보고하는 바이다. 급성 신우신염은 가와사끼병의 초기 증세일 수 있다. 그런 경우, 환아가 가와사끼병으로 확진되더라도 요로감염 진료지침에 따라 요로기형에 대한 이미지 검사를 시행할 필요가 있다고 생각한다.

• 한국미생물·생명공학회지
• /
• 제17권6호
• /
• pp.634-644
• /
• 1989

Listeria monocytogenes, one of five species in the genus Listeria and the only one currently believed to be pathogenic for humans, is a small gram-positive, nonsporeforming, aerobic, motile and hemolytic rod-shaped bacterium. The bacterium is widespread in the environment, having been isolated from soil, dust, animal feed, water, sewage, almost every type of animal that has been cultured, and asymptomatic humans. L. monocytogenes causes listeriosis, a disease which most often affects humans with a compromised immune system. Included are pregnant woman, infants and adults suffering from such diseases as cancer, cirrhosis of liver or AIDS or are being treated with drugs such as corticosteroids. Listeriosis is manifested by such syndromes as pregnancy infections, granulomatosis infantiseptica, sepsis, meningoencephalitis, and focal infections. Infections, can be treated successfully with penicillin, ampicillin, or erythromycin. However, a mortality rate of about 30％ has occurred in outbreaks of listeriosis. Food-associated outbreaks of listeriosis have been attributed to coleslaw (Canada, 1981), pasteurized milk (U.S., 1983), and soft cheese (U.S., 1985). Presence of L. monocytogenes in various dairy foods has prompted recall of such products from the U.S. market-place. L. monocytogenes also has been found in raw meats and seafood.