• 융합신호처리학회논문지
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• 제24권1호
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• pp.76-81
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• 2023

암세포와 정상세포를 구분하기 위해서는 H&E(Hematoxylin&Eosin) 염색이 필요하다. 병리 염색은 많은 비용과 시간이 필요하다. 최근 이러한 비용과 시간을 줄이고자 디지털 염색 방법이 소개되고 있다. 본 연구에서는 병리 H&E 염색의 디지털 변환 방법에 대한 새로운 알고리즘을 제안한다. 첫 번째 알고리즘은 Pair방법이다. 본 방법은 FPM(Fourier Ptychographic Microscopy)으로 촬영된 염색된 Phase 영상과 염색되지 않은 Amplitude 영상을 학습하여 염색된 Amplitude 영상으로 변환한다. 두 번째 알고리즘은 Unpair방법이다. 본 방법은 염색된 형광현미경 영상과 염색되지 않은 형광현미경 영상을 학습하여 모델링하여 디지털 염색을 수행한다. 본 연구에서는 GAN(generative Adversarial Network)를 활용하여 디지털 염색을 진행하였다. 연구 결과 Pair방법과 Unpair방법 모두 우수한 성능의 디지털 염색 결과를 확보하였다.

• 대한치과의사협회지
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• 제15권8호
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• pp.611-615
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• 1977

The author observed histochemically the nature of the calcified tissue in the rabbit dental pulp, induced by pulpal injection of potassium permanganate. The pulp of rabbit mandibular incisors were exposed and enlarged by a dental hand reamer. The exposed pulps were injected with 0.05ml of 20mM solution of potassium permanganate dissolved in Ringer's solution in experimental tooth. Also the control tooth received a pulpal injection of 0.05ml of Ringer's solution. After pulpal injection, the tooth was plugged with a gutta-percha root canal point. The staining techniques were hematoxylin-eosin stain, van Gieson stain, PAS reaction, toluidine blue stain, alcian blue-hematoxylin stain and colloidal iron-picric acid stain. The results were as follows: 1. The pulp on experimental tooth showed osteodentin-like calcified tissue. Also, in some areas, false denticle-like substance were observed. 2. The central portion of the calcified matrix showed metachromasia in toluidine blue stain had strong staining capacity in alcian blue stain. 3. The peripheral portion of the calcified tissue revealed marked van Gieson positive reaction for collagen. But their staining ability in alcian blue was slight and metachromasia was not appeared.

• 대한치과의사협회지
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• 제18권8호통권137호
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• pp.657-667
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• 1980

This study was undertaken to observe the salivary gland of the white rat when exposed to single and fractionated doses of Cobalt-60 irradiation. One hundred fifty white rats of the experiment were devided into control and 3 experimental groups. In experimental groups, group I receivcd 1200 rads everyweek untill 4800 rads reached, group II received 1500 rads and group III received 2000 rads with single dose. irradiation was carried out using a RAC-120 Cobalt-60 Teletherapy Unit with a dose rate 84.3 r/min, field size 4×5 cm measured at 80 from source. Rats were serially sacrificed at the following postirradiation time intervals: 1, 3, 5, 7, 10, 14, 21, 28, 35, and 42days. At sacrifice, the parotid and submandibular glands were dissected out in toto, and stained with: 1) hematoxylin and eosin; 2) periodic acid Shiff; 3) toludine blue.

• 대한치과의사협회지
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• 제11권12호
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• pp.783-786
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• 1973

The healing response may very with the tissue, the site and the degree of wound. the author observed histochemically the epithelial regeneration in the har palate wound of healthy male albino rats, varying in age from 120 to 150 days, and weighing about 100 gm. The deep wounds were made antero-posterior linealy by surgical knife to the depth of bone level. They were sacrified by ether anesthesia on 1, 2, 4, 7, 10 and 14 days after wounding. the staining methods used were Mcmanus' PAS reaction, Mowry's modification of the Hale reaction employing Muller's colloidal iron reagent, alloxan-Schiff reaction and hematoxylin-eosin stain. The results were as follows : 1. In the wound healing of hard palate, the epithelium had marked PAS positive reaction in the granular and the prickle cell layers on the from 2nd to 7th day. 2. Alloxan-Schiff reactions of regenerated epithelium were slightly increased on 7th day.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제42권2호
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• pp.115-119
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• 2016

Leiomyoma is a type of benign smooth muscle neoplasm that is a common neoplasm of the uterus and gastrointestinal tract but rarely affects the head and neck region and is especially unlikely to affect the oral cavity. The diagnosis of leiomyoma is mainly determined by histopathological studies due to variation in its clinical appearance and symptoms. In the present paper we report two rare cases of gingival angioleiomyoma in the posterior maxilla and mandible. After total excision, hematoxylin-eosin and smooth muscle actin staining confirmed the diagnosis of angioleimyoma.

• 대한통합의학회지
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• 제5권4호
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• pp.49-55
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• 2017

Purpose : In this study we tested the hypothesis that eucommia ulmoides (EU) extract would improve disuse-induced muscle atrophy following hindlimb immobilization. Method : The hindlimb immobilization was performed with casting tape to keep the left ankle joint in a fully extended position. The rats in EU treated group were orally administrated with eucommia ulmoides water extract. After 2 weeks of immobilization, all animals were sacrificed, and the whole gastrocnemius muscles were dissected from both legs. The morphology of right and left gastrocnemius muscles in both EU and control groups were assessed by hematoxylin and eosin staining. Results : Eucommia ulmoides extract represented the significant protective effects against the reductions of the gastrocnemius muscles weight and average cross section area to compared with Control group. Conclusion : Eucommia ulmoides has protective effects against immobilization induced muscle atrophy.

• 한국수정란이식학회지
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• 제6권1호
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• pp.41-45
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• 1991

소와 견에 있어서 Unopette가 정자의 형태학적 검사 및 정자농도의 검사를 위하여 사용될 수 있는가를 알아 보기 위하여본 연구를 수행하였다. 소정액 및 견정액을 Unopette에 희석한 후 3-5$^{\circ}C$에 보존하면서 시간경과에 따라서 위상차현미경하에서 관찰하여 다음과 같은 결과를 얻었다. 1. Unopette를 사용하여 관찰한 정자는 48시간까지는 hematoxylin-eosin을 사용하여 정자보다는 높은 정상정자율을 나타내넜다. 2. Unopette를 사용한 정자는 정자농도에 있어서 대조군 정자에 비하여 24시간까지는 큰 차이를 나타내지 않았다. 3. 따라서 Unopette는 정자의 형태학적 검사 및 정자농도의 검사를 위하여 유용하게 사용될 수 있다는 사실을 알 수 있었다.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1998년도 춘계학술발표회논문집(2)
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• pp.697-702
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• 1998

방사선에 의해 유발되는 난소내 난포의 폐쇄가 apoptosis를 매개로 하여 일어나는지 조사하고자 면역조직화학적 방법을 이용하여 실험을 수행하였다. 미성숙 생쥐 (3주, ICR)에 감마선을 조사하였으며, 조직절편을 제작한 후 TUNEL 방법에 의한 in situ 3'end labelling 면역조직화학 염색을 실시하였다. 전반적인 난소의 상태를 파악하고자, 일반적인 hematoxylin-eosin(HE) 염색을 실시하여 대조하였다. 면역조직화학 염색은 apoptosis가 일어난 난포를 시각적으로 구분해낼 수 있는 효과적인 방법임이 실험적으로 확인되었다. ApopTeg/HE 비율을 볼 때, R군은 6h에서 12h 에 높은 값을 보였고, 8d군에서 다시 증가하는 양상을 보였으며 이같은 결과는 실험동물의 난소내 과립세포의 apoptosis가 방사선 조사후 6시간부터 일어나기 시작하여 점차 증가된다는 사실을 나타낸 것이다. 결론적으로 방사선에 의한 난포의 폐쇄는 과립세포의 apoptosis를 매개로 하여 일어남을 알았다.

• 대한치과의사협회지
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• 제9권11호
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• pp.673-677
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• 1971

The buccal mucosa was observed histochemically in male rats which were injected with methionine. The staining methods were proceed by means of periodicacid-Schiff reaction, toluidine blue stain, alloxan-Schiff reaction, Mallory's aniline blue stain, Lillie's modification of Bielschowsky method and hematoxylin-eosin stain. The results of the experiment were summarized as follows: 1) PAS reactions of basement membrane and lamina propria increased after 3 and 5 days of methionine administration. 2) Metachromasia of stratum spinosum increased after 3 days of methionine administration. 3) Alloxan-Schiff reactions of stratum granulosum and stratum spinosum increased after 7 days of methionine administration. 4) In the lamina propria, aniline blue staining of collagenous fibers increased after 7, 10 and 14 days of methionine administration.

• Environmental Analysis Health and Toxicology
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• 제11권3_4호
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• pp.45-51
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• 1996

Histopathological changes of gills and caudal fins isolated from fishes, Cyprinus carpio, Carassius auratus, and Hernibarbus labeo, which were killed by oxygen deficiency or toxic chemicals, were studied. The toxic chemicals were HCl, NaOH, chloroform, benzene, heavy metals(Cu, Cr, Zn, Pb, Hg), and o-dichlorobenzene. The exposure level was enough to kill the fishes within 30 minutes. Oxygen deficient water was prepared by aeration of nitrogen gas and the oxygen concentration was less than l ppm. Cryocutting was used for the rapid preparation of tissue slides and the tissues were stained by hematoxylin/eosin. In the fishes killed by hazardous chemicals, congestion and/or hyperplasia of secondary lamella and erosion of fin were found as the major histopathological changes. Whereas, these characteristics were not observed in gills or caudal fins of fishes killed by oxygen deficiency. These different bioindications appeared in the fishes killed by toxic substances or natural causes, can be used for the rapid identification of the causes of fish kills.