• 한국보건간호학회지
• /
• 제13권1호
• /
• pp.25-40
• /
• 1999

This study was to identify various personal characteristics between those having a helper and those not having a helper for ADL and IADL performance in noninstitutionalized elders. Secondary analysis was done with the data collected in 1994 national survey of aged householders. Of the elders with ADL limitations $47.8\%$ had no helper. while $26.2\%$ of those with IADL limitations had no helper. Those elders with a helper experienced more limitations in ADL and in IADL than those with no helper. Most of the helpers were family members. whereas very few had a formal helper. Age period and subjective economic status were significantly different between the groups with a helper and with no helper for both ADL and IADL. Compared to the elders with a helper. those elders with no helper had fewer children living together. whereas they had more grandchildren. They also had more contacts with friends or neighbors. Health status. in general. was worse in those elders with a helper than those with no helper. Logistic regression analysis revealed that ADL and IADL limitations had largest influence on having an ADL helper. The odds ratios for low levels of subjective economic status were very high. As for having an IADL helper. IADL score was the most important predictor. In particular. ADL limitations had a negative effect on having an IADL helper. This finding may indicate the urgency of ADL needs. which leads to the lack of an IADL helper when both ADL and IADL limitations are present. Considering the nature of ADL. those disabled elders with low economic status need public support for their daily activities and for home visiting nursing services. There should be studies of examining the adequacy of help in satisfying basic needs for performing ADL and IADL in elders.

• BMB Reports
• /
• 제53권11호
• /
• pp.565-575
• /
• 2020

Gene therapy is emerging as a treatment option for inherited genetic diseases. The success of this treatment approach greatly depends upon gene delivery vectors. Researchers have attempted to harness the potential of viral vectors for gene therapy applications over many decades. Among the viral vectors available, gutless adenovirus (GLAd) has been recognized as one of the most promising vectors for in vivo gene delivery. GLAd is constructed by deleting all the viral genes from an adenovirus. Owing to this structural feature, the production of GLAd requires a helper that supplies viral proteins in trans. Conventionally, the helper is an adenovirus. Although the helper adenovirus efficiently provides helper functions, it remains as an unavoidable contaminant and also generates replication-competent adenovirus (RCA) during the production of GLAd. These two undesirable contaminants have raised safety concerns and hindered the clinical applications of GLAd. Recently, we developed helper virus-free gutless adenovirus (HF-GLAd), a new version of GLAd, which is produced by a helper plasmid instead of a helper adenovirus. Utilization of this helper plasmid eliminated the helper adenovirus and RCA contamination in the production of GLAd. HF-GLAd, devoid of helper adenovirus and RCA contaminants, will facilitate its clinical applications. In this review, we discuss the characteristics of adenoviruses, the evolution and production of adenoviral vectors, and the unique features of HF-GLAd as a new platform for gene therapy. Furthermore, we highlight the potential applications of HF-GLAd as a gene delivery vector for the treatment of various inherited genetic diseases.

• 대한수의학회지
• /
• 제20권1호
• /
• pp.11-15
• /
• 1980

순수하게 분리 정제된 AAAV를 "helper"인 가금 adenovirus와 공동으로 11일령의 계태아에 감염시켰으며 토끼 유래 특히 항혈청을 사용하여 AAAV의 증식성을 추시하였다. AAAV의 증식은 "helper"와의 공동감염인 경우에만 인정되었으며 "helper"의 양과 AAAV의 증식은 서로 일치하였으나, AAAV와의 공동감염인 경우 "helper"의 증식은 억제되는 경향을 보였다. 이 시험에 공여된 7주의 가금 adenovirus중 6주는 AAAV의 증식을 위한 "helper"능을 부여하였으며 "helper"로써의 기능은 계태아에서의 증식성과 일치하였다. 계태아에 순화된 (CELO) 바이러스를 "helper"로 하여 계태아에서의 AAAV의 증식을 조사한바, $10^5$ PFU의 CELO 바이러스를 "helper"로 하였을 경우 접종후 5일에 최고에 달하였다.

• 농촌지도와개발
• /
• 제12권2호
• /
• pp.219-230
• /
• 2005

The objectives of this study were 1) to evaluate level of satisfaction of the farm helper system which has been executed by Ministry of Agriculture & Forestry from the year 2000 as one of the welfare policies for assisting production activities of woman farmers. and 2) to suggest some measures for improving farm helper system. The data were collected from selected 729 women farmers who utilized the farm helper system. The major findings and suggestions from this study were as follows: 1) Women farmers responded that they had positive attitudes toward utilizing farm helper system by answering 'obtaining mental stability'(34.6%). 'reducing a burden of child birth'(29.2%), 'continuing to work on farm activities'(24.4%) and 'trust in national policies'(10.7%). 2) In addition, women farmers wanted to use the helpers for two months, before and after having a child birth. 3) They suggested that farm helpers system should be extended to other areas such as farming(21.5%), nursing(18.6%), and child-caring(16.1%).

• 한국임상수의학회지
• /
• 제8권1호
• /
• pp.1-10
• /
• 1991

The B-lymphocyte differentiation from committed B-cell progenitors to antibody-secreting cells was discussed. B-cell progenitors derived from hematopoietic stem cells undergo the rearrangement of immunoglobulin(Ig) gene. The earliest cells as B-cell precursors have cytoplasmic Is(${\mu}$ chain). The entire Is molecule is expressed on the surface after synthesis of L chain. The resting B cells(Go stage) stimulated by binding antigen via Ig-receptors are activated(G$_1$ stage) and followed by proliferation(S stage), coupled with further selection(affinity maturation. class switch). The production of antibody against a particular antigen depends on the activation of B cells with surface Is capable of reacting with that antigen. This process does not occur in isolation but is controlled by helper and suppressor T cells and antigen presenting cells(APC). The mechanism of T cell-dependent B-cell response for production of antibody is largely explained by the cell to cell cooperation and soluble helper factors of T cells. 1) The antigen specific B cells and helper T cells are linked by Is-receptors, leading to the delivery of helper signals to the B cells. 2) Helper T cells recognize the processed antigen-derived peptides with the MHC class II molecules(la antigen) and is stimulated to secrete B-cell proliferation and differentiation factors which activate B cells of different antigenic specificity. The two models are shown currently 1) At low antigen concentration, only the antigen-specific B cell binds antigen and presents antigen-derived peptides with la molecules to helper T cells, which are stimulated to secrete cytokines(IL-4, IL-5, etc.) and 2) At high antigen concentration, antigen-derived peptides are presented by specific B cells, by B cells that endocytose the antigens, as well as by APC Cytokines secreted from helper T cells also lead to the activation of B cells and even bystander B cells in the on- vironmment and differentiate them into antibody-secreting plasma cells.

• 한국인터넷방송통신학회논문지
• /
• 제16권5호
• /
• pp.111-116
• /
• 2016

미래창조과학부의 정보격차 실태조사에 따르면 장애인의 스마트폰 보유율은 일반인의 1/3 수준에 머물러 있어 장애인의 정보접근성은 비장애인에 비해 현저히 떨어지는 실정이다. 본 논문은 청각장애인의 모바일 음성 통화를 보다 편리하게 사용할 수 있도록 도와주는 어플리케이션인 CallHelper를 개발하였다. CallHelper는 전화가 오면 자동으로 구동되어 상대방의 전화음성을 텍스트로 실시간 번역하여 모바일 화면에 출력하고, 상대방의 음성에서 감정을 추론하여 이모티콘으로 시각화해서 표시하며, 음성과 번역된 텍스트, 감정을 동시에 저장하여 추후 번역결과를 확인해 볼 수 있도록 하는 어플리케이션이다.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2003년도 춘계학술발표논문집 (중)
• /
• pp.1035-1038
• /
• 2003

지식 객체를 이용하여 멀티미디어 문서를 모델링하고, 학습과 추론 설비를 사용하여 사용자의 검색을 도와주는 Localized 에이전트(SiteHelper)를 비롯한 여러 가지 에이전트들을 이용하여 진보된 서비스를 제공할 수 있는 디지털 라이브러리 시스템을 디자인한다.

• Journal of information and communication convergence engineering
• /
• 제12권4호
• /
• pp.199-207
• /
• 2014

A new cooperative MAC protocol called the busy tone cooperative medium access control (BT-COMAC) protocol is proposed to overcome the drawbacks and maximize the advantages of existing schemes. This scheme uses a new metric called decibel power to decide an appropriate helper node. Using received power strength is more efficient in selecting an appropriate helper node, especially in a densely populated network, than the effective transmission rates used in conventional schemes. All communication nodes in a communication service area are assumed to move independently. Two performance metrics are used: System throughput and channel access delay. A performance evaluation of the BT-COMAC protocol is conducted using a computer simulation over a slow fading wireless channel, and its performance results are compared with those of four existing schemes. The numerical results show that the BT-COMAC protocol improves the system throughput by approximately 15% as compared to the best existing scheme.

• Journal of Microbiology and Biotechnology
• /
• 제15권6호
• /
• pp.1346-1352
• /
• 2005

We have developed a modified gene replacement method using PCR products containing short homologous sequences of 40- to 50-nt. The method required $\lambda$-Red recombination functions provided under the control of a temperature-sensitive CI857 repressor expressed from the $P_{lac}$ promoter in the presence of IPTG on an easily curable helper plasmid. The method promoted the targeted gene replacements in the Escherichia coli chromosome after shifting cultures of the recombinogenic host, which carries the helper plasmid, to $42^{\circ}C$ for 15 min. Since this method employs $\lambda$-Red recombination functions expressed from the easily curable helper plasmid, multiple rounds of gene replacements in the E. coli chromosome would be possible. The procedures described herein are expected to be widely used for metabolic engineering of E. coli and other bacteria.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2013년도 추계학술발표대회
• /
• pp.1683-1685
• /
• 2013

이 논문에서는 환경적 장애 극복을 위한 소프트웨어 접근성 향상을 위한 시스템인 PC Helper을 제안한다. P.C Helper는 [Speech Mode]와 [Motion Mode] 두 가지의 모드로 구성된다. [Speech Mode]에서는 UI Speaking Engine을 활용하여 음성으로 응용프로그램을 제어하고, [Motion Mode]에서는 Gesture Engine을 활용하여 모션을 이용한 가상의 키보드와 마우스를 제어할 수 있다. 이 두 가지 기능을 제공하여 환경적 장애가 있는 사람들에게 용이한 인터페이스를 제공하고자 하였다. UI Speaking Mode로 Microsoft Office 응용 프로그램 약 70%정도 제어하는 것이 가능하였다.