• Title/Summary/Keyword: Helmholtz

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Antioxidant capacity in seedling of colored-grain wheat under water deficit condition

  • Kim, Dae Yeon;Hong, Min Jeong;Jung, Woo Joo;Seo, Yong Weon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.140-140
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    • 2017
  • Nutritious and functional foods from crop have received great attention in recent years. Colored-grain wheat contains high phenolic compound and a large number of flavonoid. The anthocyanin and polyphenolic synthesis and accumulation is generally stimulated in response to biotic or abiotic stresses. Here, we analyzed genome wide transcripts in seedling of colored-grain wheat response to ABA and PEG treatment. About 900 and 1500 transcripts (p-value < 0.05) from ABA and PEG treatment were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. NR protein sequences of Poaceae from NCBI and protein sequence of transcription factors originated from 83 species in plant transcription factor database v3.0 were used for annotation of putative transcripts. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. DroughtDB (http://pgsb.helmholtz-muenchen.de/droughtdb/) was used for detection of DEGs to explain that physiological and molecular drought avoidance by drought tolerance mechanisms. Drought response pathway, such as ABA signaling, water and ion channels, detoxification signaling, enzymes of osmolyte biosynthesis, phospholipid metabolism, signal transduction, and transcription factors related DEGs were selected to explain response mechanism under water deficit condition. Anthocyanin, phenol compound, and DPPH radical scavenging activity were measured and antioxidant activity enzyme assays were conducted to show biochemical adaptation under water deficit condition. Several MYB and bHLH transcription factors were up-regulated in both ABA and PEG treated condition, which means highly expressed MYB and bHLH transcription factors enhanced the expression of genes related in the biosynthesis pathways of flavonoids, such as anthocyanin and dihydroflavonols in colored wheat seedlings. Subsequently, the accumulation of total anthocyanin and phenol contents were observed in colored wheat seedlings, and antioxidant capacity was promoted by upregulation of genes involved in maintaining redox state and activation of antioxidant scavengers, such as CAT, APX, POD, and SOD in colored wheat seedlings under water deficit condition. This work may provide valuable and basic information for further investigation of the molecular responses of colored-grain wheat to water deficit stress and for further gene-based studies.

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Magnetic Resonance Elastography (자기 공명 탄성법)

  • Kim, Dong-Hyun;Yang, Jae-Won;Kim, Myeong-Jin
    • Investigative Magnetic Resonance Imaging
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    • v.11 no.1
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    • pp.10-19
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    • 2007
  • Conventional MRI methods using T1-, T2-, diffusion-, perfusion-weighting, and functional imaging rely on characterizing the physical and functional properties of the tissue. In this review, we introduce an imaging modality based on measured the mechanical properties of soft tissue, namely magnetic resonance elastography (MRE). The use of palpation to identify the stiffness of tissue remains a fundamental diagnostic tool. MRE can quantify the stiffness of the tissue thereby providing a objective means to measure the mechanical properties. To accomplish a successful clinical setting using MRE, hardware and software techniques in the area of transducer, pulse sequence, and imaging processing algorithm need to be developed. Transducer, a mechanical vibrator, is the core of MRE application to make wave propagate invivo. For this reason, considerations of the frame of human body, pressure and friction of the interface, and high magnetic field of a MRI system needs to be taken into account when designing a transducer. Given that the wave propagates through human body effectively, developing an appropriate pulse sequence is another important issue in obtaining an optimal image. In this review paper, we introduce the technical aspects needed for MRE experiments and introduce several applications of this new field.

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Biofilm Differentiation and Dispersal

  • Kjelleberg, Staffan;Barraud, Nicolas;Egan, Suhelen;Ho, Wing Ka;Huynh, Trieu Tran;Klebensberger, Janosch;Koh, Kai Shyang;Lucas-Elio, Patricia;Mai-Prochnow, Anne;Marshall, Dustin J.;Matz, Carsten;McDougald, Diane;Rice, Scott A.;Sanchez-Amat, Antonio;Schleheck, David;Shahbazi, Jeyran;Steinberg, Peter D.;Tan, Chuan Hao;Thomas, Torsten;Webb, Jermy S.;Woo, Jerry K.K.
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2008.05a
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    • pp.42-44
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    • 2008
  • Bacterial biofilms are analogous to multi-cellular organisms or to clonal communities of higher organisms. In this respect, it can be demonstrated that biofilms display the type of genetic variation associated with macroorganisms. The formation of genetic variants from biofilms is the result of internally produced and regulated signals and the appearance of these variants coincides with dispersal from the biofilm. Moreover, the generation of such variation, has similar outcomes for the bacterial community, where diversification of phenotypic traits ensures that the bacterial community optimizes its chances of success when dispersing or surviving when challenged with environmental stress. These observations increase the complexity with which we view bacteria and also suggest that microbial systems can serve as models for the testing of eukaryotic ecological theories.

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Identification of the Sectional Distribution of Sound Source in a Wide Duct (넓은 덕트 단면내의 음원 분포 규명)

  • Heo, Yong-Ho;Ih, Jeong-Guon
    • The Journal of the Acoustical Society of Korea
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    • v.33 no.2
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    • pp.87-93
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    • 2014
  • If one identifies the detailed distribution of pressure and axial velocity at a source plane, the position and strength of major noise sources can be known, and the propagation characteristics in axial direction can be well understood to be used for the low noise design. Conventional techniques are usually limited in considering the constant source characteristics specified on the whole source surface; then, the source activity cannot be known in detail. In this work, a method to estimate the pressure and velocity field distribution on the source surface with high spatial resolution is studied. The matrix formulation including the evanescent modes is given, and the nearfield measurement method is proposed. Validation experiment is conducted on a wide duct system, at which a part of the source plane is excited by an acoustic driver in the absence of airflow. Increasing the number of evanescent modes, the prediction of pressure spectrum becomes further precise, and it has less than -25 dB error with 26 converged evanescent modes within the Helmholtz number range of interest. By using the converged modal amplitudes, the source parameter distribution is restored, and the position of the driver is clearly identified at kR = 1. By applying the regularization technique to the restored result, the unphysical minor peaks at the source plane can be effectively suppressed with the filtering of the over-estimated pure radial modes.

A study on in-flight acoustic load reduction in launch vehicle fairing by FE-SEA hybrid method (FE-SEA 하이브리드 기법을 이용한 비행 중 발사체 페어링 내부 음향하중 저감에 관한 연구)

  • Choi, Injeong;Park, Seoryong;Lee, Soogab
    • The Journal of the Acoustical Society of Korea
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    • v.39 no.4
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    • pp.351-363
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    • 2020
  • Launch vehicles are subject to airborne acoustic loads during atmospheric flight and these effects become pronounced especially in transonic region. As the vibration due to the acoustic loads can cause malfunction of payloads, it is essential to predict and reduce the acoustic loads. In this study, a complete process has been developed for predicting airborne vibro-acoustic environment inside the payload pairing and subsequent noise reduction procedure employing acoustic blankets and Helmholtz resonators. Acoustic loads were predicted by Reynolds-Averaged Navier-Stokes (RANS) analysis and a semi-empirical model for pressure fluctuation inside turbulent boundary layer. Coupled vibro-acoustic analysis was performed using VA One SEA's Finite Element Statistical Energy Analysis (FE-SEA) hybrid module and ANSYS APDL. The process has been applied to a hammerhead launch vehicle to evaluate the effect of acoustic load reduction and accordingly to verify the effectiveness of the process. The presently developed process enables to obtain quick analysis result with reasonable accuracy and thus is expected to be useful in the initial design phase of a launch vehicle.

자장 강화된 유도결합형 플라즈마를 이용한 TFT-LCD용 Al-Nd 박막의 식각 특성 개선에 관한 연구

  • 한혜리;이영준;오경희;홍문표;염근영
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.195-195
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    • 2000
  • TFT-LCD의 제조공정은 박막층의 식각 공정에 대해 기존의 습식 공정을 대치하는 건식식각이 선호되고 있다. 건식 식각 공정은 반도체 공저에 응용되면서 소자의 최소 선폰(CD)이 감소함에 따라 유도결합셩 프라즈마를 비롯한 고밀도 플라즈마 이용한 플라즈마 장비 사용이 증가하는 추세이다. 여기에 평판디스플레이의 공정을 위해서는 대면적과 사각형 기판에 대한 균일도를 보장할 수 있는 고밀도의 균일한 플라즈마 유지가 중요하다. 본 실험에서는 자장강화된 유도결합형 플라즈마의 플라즈마 밀도 및 균일도를 살펴보고 TFT-LCD에 gate 전극으로 사용되는 Al-Nd 박막의 식각을 통하여 식각균일도와 식각속도 및 식각 선택도 등의 건식 식각 특성을 보고자 한다. 영구자석 및 전자석의 설치는 사각형의 유도결합형 플라즈마는 소형 영구자석을 배열하여 부착하였으며, 외부에는 chamber와 같이 사각형태의 전자석을 500mm$\times$500mm의 크기를 갖는 z축 방향의 Helmholtz형으로 제작하였다. 더. 영구자석 배열에 대해서는 자석간의 거리와 세기 변화를 조합하여 magnetic cusping의 변화를 주었으며 전자석의 세기는 전류값을 기준으로 변화시켜 보았다. 실험을 통하여 플라즈마 균일도를 5% 이하로 개선하고 이러한 균일도를 유지하며 플라즈마 밀도를 높일 수 있는 조건을 찾을 수 있었다. 이러한 적합화된 조건에서 저장강화된 유도결합형 프라즈마를 Al-Nd 박막 식각에 응용한 결과, Al-Nd의 식각속도 및 식각 선택도는 유도결합형 프라즈마에 비해 크게 증가하였으며, 식각균일도가 개선되는 것을 관찰하였다. 또한 electrostatic probe(Hiden, Analytical)를 이용하여 Al-Nd 식각에 사용된 반응성 식각가스에 대한 저장강화된 유도결합형 플라즈마의 특성 분석을 수행하였다.c recoil detection, Rutherford backscattering spectroscopy, X-ray diffraction, secondary electron microscopy, atomic force microscoy, $\alpha$-step, Raman scattering spectroscopu, Fourier transform infrared spectroscopy 및 micro hardness tester를 이용하여 기판 bias 전압이 DLC 박막의 특성에 미치는 영향을 조사하였다. 분석결과 본 연구에서 제작된 DLC 박막은 탄소와 수소만으로 구성되어 있으며, 비정질 상태임을 알 수 있었다. 기판 bias 전압의 증가에 따라 박막의 두께가 감소됨을 알 수 있었고, -150V에서는 박막이 거의 만들어지지 않았으며, -200V에서는 기판 표면이 식각되었다. 이것은 기판 bias 전압과 ECR 플라즈마에 의한 이온충돌 효과 때문으로 판단되며, 150V 이하에서는 증착되는 양보다 re-sputtering 되는 양이 더 많을 것으로 생각된다. 기판 bias 전압을 증가시킬수록 플라즈마에 의한 이온충돌 현상이 두드러져 탄소와 결합하고 있던 수소원자들이 떨어져 나가는 탈수소화 (dehydrogenation) 현상을 확인할 수 있었으며, 이것은 C-H 결합에너지가 C-C 결합이나 C=C 결합보다 약하여 수소 원자가 비교적 해리가 잘되므로 이러한 현상이 일어난다고 판단된다. 결합이 끊어진 탄소 원자들은 다른 탄소원자들과 결합하여 3차원적 cross-link를 형성시켜 나가면서 내부 압축응력을 증가시키는 것으로 알려져 있으며, hardness 시험 결과로 이것을 확인할 수 있었다. 그리고 표면거칠기는 기판 bias 전압을 증가시킬수록 더 smooth 해짐을 확인하였다.인하였다.을 알 수 있

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An Empirical Study of Soundproof wall with Reduced Wind Load (풍하중 저감형 방음판의 실증 연구)

  • Choi, Jin-Gyu;Lee, Chan-Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.12
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    • pp.272-278
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    • 2018
  • Traffic volume has been greatly increasing due to urban development and the improvement of living standards, and many complaints are being raised due to the increasing road noise. As a countermeasure against these problems, highly soundproof walls are installed on the sides of roads. However, the ability to bear wind loads is a major design requirement for soundproof walls, which contributes to the exponential increases in construction costs and restricts the height of the walls. The aim of this study is to improve the performance of soundproof walls and to dramatically reduce wind loads while maintaining excellent price competitiveness. Based on Helmholz's resonator theory, a new concept is proposed for a ventilation-type soundproofing plate that can pass through a fluid like air and reduce noise. A full-scale metal soundproofing plate was produced to satisfy the quality standards of highways by conducting a sound-pressure transmission-loss test, wind tunnel test, and material quality test. To verify the reliability, the wall was manufactured and installed, and the sound insulation effect was examined by measuring the noise over time. In the future, ventilated soundproof walls on roads could create a pleasant living environment due to the high noise-insulation effect.

A method of wall absorption treatment for enhancing the speech intelligibility at a directional microphone array in a room (실내 공간 내 지향성 마이크 어레이에서의 음성 명료도 개선을 위한 벽면 흡음 처리 방법)

  • Ko, Byeong-Yun;Ih, Jeong-Guon;Cho, Wan-Ho
    • The Journal of the Acoustical Society of Korea
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    • v.40 no.6
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    • pp.649-659
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    • 2021
  • Wall absorption treatment effectively reduces reverberation, but requires a large area for a live room and each wall absorption affects speech intelligibility differently. In this study, we try to find the most effective wall for the absorption treatment using the beamforming array microphone in terms of speech intelligibility. The absorption importance factor is defined by using the collision number of reflected sounds on each wall. It allows estimating how much the speech signal will be enhanced by the absorption treatment. A cuboid room with a size of 107 m3 and a reverberation time of 1.1 s is selected for the simulation. When a Helmholtz-type absorption is treated on the wall with the most significant importance factor, the modified clarity for 500 and 1k Hz is improved by 5.1 dB and 4.8 dB respectively, and the speech transmission index is enhanced by 0.06. The difference in results between the proposed method and commercial simulation code is less than a Just-Noticeable Difference (JND). The absorption treatment on the wall with the most significant importance factor shows improvement greater than the wall with the largest area, and its difference is larger than a JND value.