• 대한기계학회논문집A
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• 제29권6호
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• pp.904-912
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• 2005

The flow in a microchannel is usually characterized as a low Reynolds number (Re) so that good mixing is quite difficult to be achieved. In this regard, we developed a novel chaotic micromixer, named Barrier Embedded Kenics Micromixer (BEKM). In the BEKM, the higher level of chaotic mixing can be achieved by combining two general chaotic mixing mechanisms: (i) splitting/reorientation by helical elements inside the microchannel and (ii) stretching/folding via periodically located barriers on the channel wall. The fully three-dimensional geometry of BEKM was realized by a micro-stereolithography technology, in this study, along with a Kenics micromixer and a circular T-pipe. Mixing performances of three micromixers were experimentally characterized in terms of an average mixing color intensity of phenolphthalein. Experimental results show that BEKM has better mixing performance than other two micromixers. Chaotic mixing mechanism, proposed in this study, could be integrated as a mixing component with Micro-Total-Analysis-System, Lab-on-a-chip and so on.

• 한국자기공명학회논문지
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• 제11권2호
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• pp.85-94
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• 2007

Vanilloid receptor I [transient receptor potential vanilloid subfamily member 1 (TRPV1), also known as VR1] is a non-selective cationic channel activated by noxious heat, vanilloids, and acid, thereby causing pain. VR1 possesses six transmembrane domain and N-and C-terminus cytosolic domains, and appears to be a homotetramer. We studied the structural properties of Cterminus of VR1 (VR1C) using CD and NMR spectroscopy. DPC micelles, with a zwitterionic surface, and SDS micelles, with a negatively charged surface, were used as a membrane mimetic model system. Both SDS and DPC micelles could increase the stability of helical structures and/or reduce the aggregation form of the VR1C. However, the structural changing mode of the VR1C induced by the SDS and DPC micelles was different. The changes according to the various pHs were also different in two micelles conditions. Because the net charges of the SDS and DPC micelles are negative and neutral, respectively, we anticipate that this difference might affect the structure of the VR1C by electrostatic interaction between the surface of the VR1C and phospholipids of the detergent micelles. Based on these similarity and dissimilarity of changing aspects of the VR1C, it is supposed that the VR1C probably has the real pI value near the pH 7. Generally, mild extracellular acidic pH ($6.5{\sim}6.8$) potentiates VRI channel activation by noxious heat and vanilloids, whereas acidic conditions directly activate the channel. The channel activation of the VRI might be related to the structural change of VR1C caused by pH (electrostatic interactions), especially near the pH 7. By measuring the $^1-^{15}N$ TROSY spectra of the VR1C, we could get more resolved and dispersed spectra at the low pH and/or detergent micelles conditions. We will try to do further NMR experiments in low pH with micelles conditions in order to get more information about the structure of VR1C.

• 한국정보처리학회논문지
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• 제7권8호
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• pp.2473-2483
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• 2000

본 논문에서는 영상 통신 채널 상에서 발생하는 잡음을 효과적으로 제거하기 위해 터보코드를 사용하였다. 터보코드는 복호 성능이 우수하지만 시스템의 복잡도와 복호 과정의 시간지연 때문에 실시간 통신에는 부적합하다는 단점이 있다. 이 문제를 극복하기 위해, 본 논문에서는 터보코드의 부·복호기에 사용되는 인터리버의 크기를 감소시켜 영상 데이터를 전송 할 때 소요되는 시간지연을 줄이는 새로운 세미 랜덤(Semi-Random)인터리버 알고리즘을 제안하였다. 세미 랜덤 인터리버 알고리즘은 입력 프레임의 길이를 1/2 크기만큼 인터리버를 구성하고, 인터리버 내에 데이터를 입력할 때는 블록 인터리버 처럼 행으로 입력하며, 데이터를 읽을 때는 랜덤하게 읽음과 동시에 다음 데이터가 그 주소 번지에 위치하게 된다. 그러므로, 기존의 블록, 대각, 랜덤 인터리버와 알고리즘의 복잡도를 비교할 시 그 복잡도가 1/2로 감소되어 세미 랜덤 인터리버를 터보코드에 적용할 때 영상 데이터를 실시간 처리할 수 있다.

• The Korean Journal of Physiology
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• 제13권1_2호
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• pp.41-50
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• 1979

The activation mechanism of K-induced contracture was studied in renal vascular muscle which does not generate an action potential readily and in taenia coli which generates a spike potential spontaneously. Helical strips of arterial muscle from rabbit renal arteries and longitudinal strips of taenia coli from guinea-pig's colons, respectively, were prepared. All experiments were performed in Tris-buffered Tyrode solution which was aerated with 100% $O_2$ and kept $35^{\circ}C$. Renal arterial muscles developed the contracture rapidly, which was composed of a small phasic and a large tonic components, when exposed to a 40 mM K-Tyrode solution. In the absence of external $Ca^{++}$, however, no K-contracture appeared. The contracture induced by K-depolarization was abolished by the treatment with verapamil, which is known to be a selective $Ca^{++}-blocker$ through potential-sensitive $Ca^{++}-channel$. K-contracture of taenia coli showed the contracture composed of a large phasic and a small tonic components. In the $Ca^{++}-free$ Tyrode solution, only the tonic component was abolished and almost no change in the phasic component was observed. The amplitude of tonic component was dependent on the external $Ca^{++}$; The tonic component increased dose-dependently by a stepwise increase of the external $Ca^{++}$, and this component decreased in parallel with the increase of verapamil in the external medium. The results of this experiment suggest that K-contracture of rabbit renal artery is the direct result of the influx of the external $Ca^{++}$, while that of taenia coli is the result of both $Ca^{++}$ influx and the release of sequestered $Ca^{++}$.

• The Korean Journal of Physiology
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• 제24권2호
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• pp.293-303
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• 1990

The contractile action of barium $(Ba^{2+})$ was investigated in the arterial strip of rabbit renal artery. The helical strip of isolated renal artery was immersed in the Tris-buffered Tyrode's solution equilibrated with 100% $O_2$ at $37^{\circ}C$ and its isometric tension was measured. $Ba^{2+}-induced$ contraction of arterial strip was dose-dependent and its maximal tension corresponded to $92.1{\pm}4.5%$ of tension by $K^+(100\;mM)$. $Ba^{2+}-induced$ contraction did not show the tachyphylactic phenomenon in the normal Tyrode's solution. $Ba^{2+}$ induced the tonic contraction in the $Ca^{2+}-free$ tyrode's solution and that was increased by the extracellula addition of $Ca^{2+}$. During the repeated exposure of the same dose of $Ba^{2+}\;(10\;mM)$ in the $Ca^{2+}-free$ Tyrode's solution, $Ba^{2+}-induced$ contraction was progressively decreased. Even though the intracellular NE-and caffeine-sensitive $Ca^{2+}$ was depleted, $Ba^{2+}$ induced the tonic contraction. After the pretreatment of lanthnum or verapamil, $Ba^{2+}$ did not induce contraction. $Ba^{2+}-induced$contraction was suppressed by extracellular $K^+$ in the normal Tyrode's solution and that was dependent on $K^+$ concentration. Suppressive effect of $K^+\;(14\;mM)$ on the $Ba^{2+}-induced$ contraction was also dependent on the intracellular $Ca^{2+}$ concentration. From the above resuts, it is suggested that $Ba^{2+}$ activate indirectly the contractile process by promoting the mobilization of intracellular $Ca^{2+}$ and the influx of extracellular $Ca^{2+}$. It is also suggested that action of $Ba^{2+}$ on the $Ca^{2+}-activated$ $K^+$ channel can result in the depolarization of cell membrane in the rabbit renal artery.

• 폴리머
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• 제33권6호
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• pp.561-568
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• 2009

소형 플라스틱 부품들은 대부분 다수 캐비티 빼기 사출금형에서 성형된다. 이러한 다수 빼기 캐비티 금형에서의 사출성형은 캐비티간의 흐름 균형이 중요하다. 캐비티간 흐름의 불균형은 성형품의 캐비티간 물성 및 품질의 편차를 초래한다. 캐비티간의 흐름균형은 런너와 게이트에서의 흐름균형을 통하여 이루어지게 되는데 런너와 게이트에서 기하학적인 균형을 이루어도 열적 불균형으로 캐비티간 흐름의 불균형을 초래한다. 본 연구에서는 캐비티간 흐름의 균형을 위해 고안한 스크류 타입의 런너을 이용하여 캐비티간 충전균형을 고찰하였다. 여러 형태의 스크류 런너에서 결정성 수지와 비결정성 수지, 그리고 점도가 높은 수지와 점도가 낮은 수지를 이용하여 캐비티간 흐름을 관찰하였다. 흐름의 균형은 성형조건 중 사출속도에 따라 다르게 나타나기 때문에 사출속도를 변화해 가며 관찰하였다. 실험 결과를 성형해석과 비교 검토하였으며 서로 잘 일치함을 확인할 수 있었다. 스크류 런너에서 수지가 흐르면서 스크류 채널을 따라 회전운동을 하여 스크류 단면에서 온도가 균일하게 됨을 확인하였다. 그리구 이러한 균일한 온도 때문에 캐비티간 흐름 균형이 이루어지고 있음을 확인하였다. 결론적으로 실험에 사용된 새롭게 고안된 스크류 타입의 런너는 캐비티간 충전균형을 이루는데 매우 효과적임을 실험과 해석을 통해 검증할 수 있었다.