Hiroyuki Kokawa;Masayuki Shimada;Wang, Zhan-Jie;Yutaka S. Sato
Proceedings of the KWS Conference
/
2002.10a
/
pp.250-254
/
2002
Intergranular corrosion of austenitic stainless steels is a conventional and momentous problem during welding and high temperature use. One of the major reasons for such intergranular corrosion is so-called sensitization, i.e., chromium depletion due to chromium carbide precipitation at grain boundaries. Conventional methods for preventing sensitization of austenitic stainless steels include reduction of carbon content in the material, stabilization of carbon atoms as non-chromium carbides by the addition of titanium, niobium or zirconium, local solution-heat-treatment by laser beam, etc. These methods, however, are not without drawbacks. Recent grain boundary structure studies have demonstrated that grain boundary phenomena strongly depend on the crystallographic nature and atomic structure of the grain boundary, and that grain boundaries with coincidence site lattices are immune to intergranular corrosion. The concept of "grain boundary design and control", which involves a desirable grain boundary character distribution, has been developed as grain boundary engineering. The feasibility of grain boundary engineering has been demonstrated mainly by thermomechanical treatments. In the present study, a thermomechanical treatment was tried to improve the resistance to the sensitization by grain boundary engineering. A type 304 austenitic stainless steel was pre-strained and heat-treated, and then sensitized, varying the parameters (pre-strain, temperature, time, etc.) during the thermomechanical treatment. The grain boundary character distribution was examined by orientation imaging microscopy. The intergranular corrosion resistance was evaluated by electrochemical potentiokinetic reactivation and ferric sulfate-sulfuric acid tests. The sensitivity to intergranular corrosion was reduced by the thermomechanical treatment and indicated a minimum at a small roll-reduction. The frequency of coincidence-site-lattice boundaries indicated a maximum at a small strain. The ferric sulfate-sulfuric acid test showed much smaller corrosion rate in the thermomechanically-treated specimen than in the base material. An excellent intergranular corrosion resistance was obtained by a small strain annealing at a relatively low temperature for long time. The optimum parameters created a uniform distribution of a high frequency of coincidence site lattice boundaries in the specimen where corrosive random boundaries were isolated. The results suggest that the thermomechanical treatment can introduce low energy segments in the grain boundary network by annealing twins and can arrest the percolation of intergranular corrosion from the surface.
Park, Jong-Ok;Yoon, Mi-Sun;Cho, Eun-Jung;Kim, Hee-Sook;Ryu, Byung-Ho
Korean Journal of Food Science and Technology
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v.31
no.5
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pp.1378-1385
/
1999
The antioxidative activity of Fermented Anchovy on linoleic acid autooxidation was investigated in an aqueous system at pH 7.0. All solvent fractions from Fermented Anchovy were exhibited the strong antioxidative activity. Especially, BuOH and aqueous fractions were gained large amounts with strong antioxidative activity. Ultrafiltration, dialysis, heat treatment of aqueous fraction indicated that water-soluble antioxidants of Fermented Anchovy were heat-resistant, amino acid related compounds with smaller molecular weights than 1,000. Unbound fractions from DE-52 anion exchange chromatography were exhibited antioxidative activity with or without $15\;{\mu}M\;Fe^{+++}\;ion$. We were able to purify one methionine derivative from lots of antioxidative substances in Fermented Anchovy aqueous fraction by gel filtration, anion-exchange chromatography, TLC and HPLC, successfully. These data suggest that Fermented Anchovy aqueous fraction is a mixture of fermented small molecules with strong antioxidative activities.
A total of 108 strains of C jejuni isolated from pigs and chickens were examined for the susceptibility to 10 antimicrobial agents and normal sera of cattle, sheep, guinea pigs and chickens. Minimal inhibitory concentration(MIC) ranges of antimicrobial agents to C jejuni isolates were $${\leq_-}1.56$$ to $${\geq_-}100{\mu}g/ml$$ for erythromycin, rifampin, streptomycin and tetracycline, 50 to $${\geq_-}100{\mu}g$$ for cephalothin, $${\leq_-}1.56$$ to $50{\mu}g$ for ampicillin, $${\leq_-}1.56$$ to $25{\mu}g$ for kanamycin and nalidixic acid, $${\leq_-}1.56$$ to $12.5{\mu}g$ for chloramphenicol and gentamicin. Resistance rates of C jejuni were showed to in order of rifampin(84.7%), tetracycline(56.2 %), erythromycin(17.1%) and ampicillin(3.8%), all of the strains were sensitive to chloramphenicol, gentamicin and kanamycin, and the incidence rates of resistant C jejuni were highly frequent in pig isolates than chicken isolates. The drug resistance patterns of 87 chicken isolates C jejuni to 9 antimicrobial drugs were showed 12 patterns, and Sm Ra Tc(24.1%), Sm Ra(21.8%) and Ra Tc(14.9%) were relatively common, and also 21 pig isolates were showed 6 patterns and Em Sm Ra Tc(57.1%) were most frequent. The majority of the isolates showed multiple drug resistance. Bactericidal activity of 10% normal sera from healthy animals were examined for 60min at $37^{\circ}C$. C jejuni were decreased from 0.4 to 1.0 ${\log}_{10}$(p<0.01), and serum susceptibility were high in order of guinea pig, sheep, chicken and cattle sera. Serum sensitivity of C jejuni Ch-39 strain in increased serum concentation up to 10, 20, 40 and 80% were highly significant. In the normal animal serum, the number of Ch-39 strain were decreased from $1.8{\times}10^4/ml$ to $2.7{\times}10^3/ml$ after 60 min incubation(p<0.01), but the numbers were decreased to $6.6{\times}10^3/ml$ in the heat inactivated normal serum for 30 min at $56^{\circ}C$. Bactericidal activity was restored in the heat inactivated normal serum after the serum of complement source was added.
Rubber chemicals such as accelerators, antidegradants, vulcanizing agents, processing agents and retarders are very important to the production and protection of tires and rubber goods. The use of accelerators and antidegradants are evaluated in various tire components. This paper will focus on how to vulcanize tires economically and maintain the physical properties of each tire component without severe degradation due to oxygen, heat and ozone. Also, new non-nitrosoamine accelerators and non-staining antiozonants will be discussed. Lastly, the future requirements of antidegradants and accelerators in the tire industry will be reviewed. Tires have been vulcanized with Sulfenamides as primary accelerators and either Guamdine's or Thiurams as secondary accelerators to achieve proper properties at service conditions. However, interior components such as the carcass can be vulcanized with Thiazoles as a primary accelerator to cure faster than the external components. Using the combination of Sulfenamide with secondary accelerators in a tire tread compound and the combination of a Thiazole and Guanidine in a carcass compound will be presented with performance data. Uniroyal Chemical and another Rubber Chemical Manufacturer have developed, "Tetrabenzyl Thiuram Disulfide," (TBzTD) as a non-Nitrosoamine accelerator, which could replace Nitrosoamine generating Thiurams. This new accelerator has been evaluated in a tread compound as a secondary accelerator. Also, Flexsys has developed N-t-butyl-2-benzothiazole Sulfenamide (TBSI) as a non-Nitrosoamine accelerator which could replace 2-(Morpholinothio) -benzothiazole (MBS), a scorch delayed Sulfendamide accelerator. TBSI has been evaluated in a Natural Rubber (NR) belt skim compound vs. MBS. An optimum low rolling resistant cure system has been developed in a NR tread with Dithiomorpholine (DTDM). Also, future requirements for developing accelerators will be discussed such as the replacement of DTDM and other stable crosslink systems. Antidegradants are divided into two different types for use in tire compounds. Internal tire compounds such as apex, carcass, liner, wire breaker, cushion, base tread and bead compounds are protected by antioxidants against degradation from oxygen and heat due to mechanical shear. The external components such as sidewall, chafer and cap tread com-pounds are protected from ozone by antiozonants and waxes. Various kinds of staining and non-staining antioxidants have been evaluated in a tire carcass compound. Also, various para-phenylene diamine antiozonants have been evaluated in a tire sidewall compound to achieve the improved lifetime of the tire. New non-staining antiozonants such as 2, 4, 6-tris-(N-1, 4-dimethylpentyl-p-phenylene diamine) 1, 3, 5 Trizine (D-37) and un-saturated Acetal (AFS) will be discussed in the tire sidewall to achieve better appearance. The future requirements of antidegradants will be presented to improve tire performance such as durability, better appearance and longer lasting tires.
Park, Eun-Hee;Kim, Joung-A;Choi, Seung-Hwa;Bin, Jae-Hun;Cheigh, Hong-Sik;Suk, Dong-Hee;Lee, Su-Chul;Kim, Young-Hui
Journal of Life Science
/
v.17
no.6
s.86
/
pp.811-815
/
2007
ln this study we isolated 27 isolates of Campylobacter jejuni from stool samples of 882 diarrheal patients. The seasonal distribution of patients was highest at July (11.7%). All the isolates of C. jejuni hydrolyzing sodium hippurate were serotyped on basis of heat-stable antigens, and identified with the use of passive hemagglutination assay. A total of 59.3% among 27 C. jejuni isolates were identified into 6 different serotypes, which serotype HS2, HSl/44, and HS2l were dominant. Antibiotics resistant rates of C. jejuni isolates were shown to be 100%, 63.0%, 51.9%, 37.0%, 33.3%, 25.9% and 7.4% to cephalothin, trimethoprim- sulfamethoxazole, tetracycline, ciprofloxacin, ampicillin, gentamycin and clindamycin, respectively. All isolates were sensitive to the erythromycin and imipenem.
Isoflavone (daidzein and genistein) contents, and antioxidative and fibrinolytic activities of seven commercial Korean cooking-with-rice soybeans, including Seonbikong, Chungtae, Gangnamkong, Whangtae, Geodoo, Seolitae, and Wooltalikong were investigated. Daidzein and genistein were not found in Selitae, nor was didzein in Gangnamkong and Geodoo. Total daidzein and genistein levels in Chungtae and Whangtae were 500 and 1550 mg per kg, respectively. Wooltalikong, Whangtae, and Gangnamkong had very high electron donation abilities, over 90%, but Seonbikong and Chungtae showed significantly lower activities. SOD-like activities were also the highest in Gangnamkong and Wooltalikong. Fibrinolytic activities in Seonbikong, Whangtae, and Gangnamkong were similarly strong. Fibrinolytic substances purified from protease inhibitors or activated under various pH or heat treatment conditions, were different among the soybean varieties. This study revealed that, although several cooking-with-rice soybeans were poor in isoflavones, Wooltalikong and Gangnamkong could be good sources for functional products due to their strong antioxidative activities, and heat- and acid-resistant proteolytic abilities.
Journal of the Korean Crystal Growth and Crystal Technology
/
v.29
no.2
/
pp.61-70
/
2019
Geopolymer is an eco-friendly construction material that has various advantages such as reduced $CO_2$ emission, fire resistance and low thermal conductivity compared to cement. However, it has not been many studies on the thermal behavior of the surface of the geopolymer panel when flame is applied to the surface. In this study, surface characteristics of hardened geopolymer on flame exposure was investigated to observe its characteristics as heat-resistant architectural materials. External structure changes and crack due to the heat shock were not observed during the exposure on flame. According to the residue of calcite and halo pattern of aluminosilicate gel, decarboxylation and dehydration were extremely limited to the surface and, therefore, it is thought that durability of hardened geopolymer was sustained. Gehlenite and calcium silicate portion was inversely proportional to quartz and calcite and significantly directly proportional to BFS replacement ratio. Microstructure changes due to the thermal shock caused decarboxylation and dehydration of crystallization and it was developed the pore and new crystalline phase like calcium silicate and gehlenite. It is thought that those crystalline phase worked as a densification and strengthening mechanism on geopolymer panel surface.
Present studies were carried out for breeding cold resistant clones of Citrus, improving overwintering techniques of Citrus in Jeju island as well as other southern region, Result obtained were as follows 1. In the vinyl house covered with two sheets of straw mat, 12 indivuals were found as non-injury and 15 were slightly injured by leaf freeging test at $-9^{\circ}C$ for 2 hours treatment. 2. In the condition of vinyl house covered with straw mat and viny film mulching and heat-in by sun-light, the inside temperature of vinyl house were not lowered below $-3^{\circ}C$ and the ground temperatura in vinyl house keeps above $0^{\circ}C$ during winter though outdoor temperature were lower by $-15^{\circ}C$(Daejeon area). 3. The vinyl tunnel inside the vinyl house and vinyl film mulching on ground position showed greater effectiveness for preventing heat loss from house but there were no significant difference between the color of vinyl film covered the tunnel. 4. In the vertical distribution of maximum temperature in vinyl house, the upper space was slightly higher than the lower position at high temperature condition, while minimum temperature was distributed as higher as the middle position, ground surface and upper position in order at low temperature condition 5. In the horizontal distribution of temperature in vinyl house, ground and surface-temperature of north side was lower than the other sides, and citrus planted within 30cm from north side wall died by cold injury and in the other side near wall appeared slight symptom of cold injury. 6. The insulating trench ($30{\times}30{\times}30cm$) packed with straw bundle installed under north wall might be effective to prevent heat loss of ground temperature. 7. In cloudy and snowy day, the temperature difference between indoor and outdoor were less, and the indoor temperature were maintained highly during night due to the effect of prevention of heat loss. 8. The highest temperature in a day was observed at about P.M. 3 both inside and outside of vinyl house and the lowest temperature was observed at about A.M. 6. The difference between the highest and lowest temperature of indoor in a sunny day was $19^{\circ}C$, compared with $9^{\circ}C$ on a cloudy or snowy day in late November. Especially, lowering of temperature in a snowy day was so less that the curve of temperature change was comparatively constant, 9. If the effective methods of citrus cultivation in vinyl house with improved clone such as hardiness. semi-dwarf and spur type are applied, it might be possible to cultivate the citrus tree safely in Jeju island as well as other southern rejion and to magnity the cultivation of citrus tree.
Cho, Eun Jung;Park, Ji Ae;Choi, Eun Sik;Sohn, Sea Hwan
Korean Journal of Poultry Science
/
v.43
no.2
/
pp.77-88
/
2016
To establish a new synthetic Korean meat chicken breed, we tested $5{\times}5$ diallel cross mating experiment with domestic chicken breeds. Comparing stress responses among diallel crossed chicken breeds, we analyzed telomere length, DNA damage and expressions of heat shock protein genes (HSPs) as the markers of the stress response. The telomere length was measured by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes. The expression levels of HSP-70, $HSP-90{\alpha}$ and $HSP-90{\beta}$ genes were analyzed by quantitative real-time polymerase chain reaction in lymphocytes. The DNA damage rate of lymphocytes was quantified by the comet assay known as the single cell gel electrophoresis. In results, there were significant differences in the values of the stress markers such as telomere length, HSPs and DNA damage rate, and also were significant differences in viabilities and body weights among the $5{\times}5$ diallel crossed chicken breeds. The telomere shortening rate, expression values of HSPs and DNA damage rate were significant low in W and Y crossed chickens compare to the others, but GG pure breed showed the highest values in the 25 crossed chickens. Estimating correlation coefficient, the survival rate positively correlated to telomere length, but negatively correlated to the expression levels of HSP-70, $HSP-90{\alpha}$, $HSP-90{\beta}$ genes and to the value of % DNA in tail as DNA damage rate. The expression levels of HSP-70, $HSP-90{\alpha}$ and $HSP-90{\beta}$ genes of dead chickens had significantly higher than those of survival chickens. According to the results on the stress marker analysis, it would be considered that the crossed breeds had more stress resistant than the pure breeds, and the crossed chickens with a light strain such as W or Y were relatively resistant to stress, but the crossed chickens with a heavy strain such as G, H, F were susceptible to stress.
Melatonin is one of the functional hormones in the milk and found in all mammalian species. The primary motivation for the use of melatonin as a supplement is as a natural aid to better sleep. Melatonin contents in milk vary according to the naturally occurring factors such as seasons and milking times. As a number of studies indicate melatonin supplementation helps to reduce the age-related decline in hormone production and overall enhancement of health, the more detailed studies are required to know the nature of milk derived hormones and to clarify them as a functional value adaptation. The objectives of this study were to examine the change of melatonin content in bovine milk during different milking times at the two dairy farms and during dairy process including homogenization and pasteurization. Commercial dairy products including night time milking products(night milk) marketed in Japan were also investigated. Melatonin content was determined by radioimmunoassay using 125I. Individual milk was collected from lactating Holstein cows at Kyong-Ki province in Korea. At farm A, the melatonin contents milking at 4 a.m. was higher than those of 7 p.m. and 12 p.m.($6.90{\pm}3.55,\;2.01{\pm}1.47$ and $0.16{\pm}0.04pg/mL$, respectively). At farm B, the mean melatonin contents milked from 24:00 AM to 05:00 AM have shown the highest contents ($4.65{\pm}$0.72pg/mL), and milking samples of the rest time had very little or non-detection of melatonin contents. Melatonin contents of commercial night milk samples were about 5 times higher than those of other common milk products. These results suggested that melatonin might be heat-resistant and commercial dairy process might not affect its contents in the final products.
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