• Journal of Biomedical and Translational Research
• /
• v.19 no.4
• /
• pp.65-72
• /
• 2018

The purpose of this study was to investigate probiotic characteristics and immune enhancing effects of Bifidobacterium (B.) longum KBB1-26 and BIF-4, B. breve KBB5-22 isolated from human intestine for probiotic use in humans and animals. We measured acid, bile and heat tolerance, antimicrobial activity against pathogenic bacteria, Escherichia (E.) coli, Salmonella (S.) Enteritidis, Staphylococcus (S.) aureus, and Listeria (L.) monocytogenes. Immune enhancing effects of B. longum and B. breve were investigated by measuring nitric oxide (NO), nuclear factor ($NF-{\kappa}b$), $interleukin-1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6), interleukin-12 (IL-12) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) in RAW 264.7 cells or RAW BLUE cells. B. longum KBB1-26 was survived at pH 2.0. B. longum KBB1-26 and BIF-4, B. breve KBB5-22 also showed tolerance to 0.3% of oxgall bile salt. B. longum KBB1-26 was able to survive at $70^{\circ}C$ and $80^{\circ}C$ for 20 min. KBB1-26 showed the antimicrobial inhibition zone to pathogenic bacteria such as E. coli (12 mm), S. Enteritidis (14 mm), S. aureus (14 mm) and L. monocytogenes (41 mm). The production of NO ($4.5{\pm}0.00{\mu}M/mL$) and $IL-1{\beta}$ ($39.7{\pm}0.55pg/mL$) of KBB1-26 significantly higher than BIF-4 and KBB5-22, respectively. In addition, KBB1-26 and KBB5-22 induce the production of high level of $TNF-{\alpha}$ and IL-6 in macrophages. Collectively, B. longum KBB1-26 have acid, bile, heat tolerance, antimicrobial activity and immune enhancing effects. These results suggest that KBB1-26 can be used as probiotics for humans and animals.

• Horticultural Science & Technology
• /
• v.32 no.2
• /
• pp.142-148
• /
• 2014

Effect of silicon (Si) nutrition on growth and temperature stress tolerance of Nephrolepis exaltata 'Corditas' grown in a soilless substrate was examined. In vitro-grown acclimatized plantlets were transplanted into the pots containing a coir-based substrate. A nutrient solution containing 0, 50, or $100mg{\cdot}L^{-1}$ Si was supplied through a drip-irrigation system. After 5 months of cultiv ation, S i-treated and -untreated p lants were grown at 10, 25, or $40{\pm}1^{\circ}C$ under a 12 h photoperiod with $530{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and 60% RH. After 7 days, chlorophyll content and chlorophyll fluorescence parameters were measured. Silicon nutrition had a negative effect on growth characteristics of N. exaltata 'Corditas'. However, Si-treated plants had more tolerance to temperature stress than the control plants. The Fv/Fm value was not significantly different when the plants were exposed to $25^{\circ}C$. However, significant difference in Fv/Fm was recorded when plants were exposed to 10 or $40^{\circ}C$. Thus, Fv/Fm could be used as an indicator of low and high temperature tolerance in ferns. The present study also suggests that application of Si may be used to enhance temperature tolerance of ferns.

• Journal of Oral Medicine and Pain
• /
• v.32 no.4
• /
• pp.413-419
• /
• 2007

The aims of this study were to investigate the relationships among several types of thermal pain thresholds, and pressure pain thresholds. This study was designed to examine whether there were associations among different types of pain thresholds, and among different recording sites for each pain threshold measurement. Pain sensitivity thresholds including cold pain threshold (CPT), heat pain threshold (HPT), heat pain tolerance threshold (PTT), and pressure pain threshold (PPT) of 56 subjects with symptoms of temporomandibular disorders were measured on temporal muscle, masseter muscle, TMJ, and tibial areas. Thermal pain thresholds including CPT, HPT, and PTT did not show any gender differences. However, women showed significantly lower PPTs than men on all recording sites. Three thermal pain thresholds including CPT, HPT, and PTT showed weak to high correlations on all the recording sites (r= 0.324 to 0.754, p<0.05). PPTs did not show any significant correlations between each thermal pain threshold. The pain threshold of each recording site showed weak to high correlations in all pain threshold measures (r= 0.284 to 0.878, p<0.05). Our study demonstrated that thermal pain thresholds, and pain tolerance thresholds were significantly correlated, but did not show any correlation between thermal pain thresholds and pressure pain thresholds. There were relatively high correlations among the pain thresholds of different recording sites.

• Journal of Mechanical Science and Technology
• /
• v.18 no.10
• /
• pp.1809-1818
• /
• 2004

The dependence of the ignition timing in an HCCI engine on intake temperature and pressure, equivalence ratio, and fuel species is investigated with a zero-dimensional model combined with a detailed chemical kinetics. The accuracy of the model is evaluated by comparing measured and computed results in a propane-fueled HCCI engine. It is shown that the peak pressure values are reproduced within 10% and ignition timing within 5$^{\circ}$ CA. The heat loss through the walls is found to affect significantly on the ignition timing for different inlet conditions. It is also shown that for the propane-fueled engine, the tolerance in intake temperatures is 20-25K and the tolerance in intake pressure is about 1 bar for stable operation without misfire or too early ignition. Comparison of propane and heptane fuels indicates that the tendency to misfire when heptane is employed as the fuel is less than that when propane is employed with the same wall temperature conditions. However, the heptane-fueled engine may have a lower compression ratio to avoid too early ignition and hence lower efficiency. For the selected set of engine parameters, stable operations might be achieved for the heptane-fueled engine with twice as much tolerance in intake temperatures as for the propane-fueled engine.

• Journal of Plant Biotechnology
• /
• v.37 no.4
• /
• pp.400-407
• /
• 2010

Zoysiagrass (Zoysia japonica Steud.), also called Korean or Japanese lawngrass, is the most popular warm-season turfgrass in Korea and is widely used for home lawns, parks, roadsides, golf courses and athletic fields. Its use is rapidly expanding in Korea and the other countries, due to its excellent characteristics which include tolerance to heat, drought and salinity. As the utilization area of this turfgrass increases, there is an increase in the demand for improved cultivars with disease and insect tolerance or with herbicide-tolerance or with extended greening periods. Conventional breeding methods have been used to improve the traits described above with limited success. However, with the advances in biotechnology, genetic transformation can be utilized for turfgrass improvement. In this paper, we review recent progress in biotechnological improvement of zoysiagrass and discuss future molecular breeding of this species.

• KSBB Journal
• /
• v.29 no.2
• /
• pp.81-86
• /
• 2014

Lactobacilli, the dominant species of microorganisms in the vaginal flora of healthy women, play important roles to prevent bacterial vaginosis and other sexually transmitted diseases. In this study, we carried out studies on stress adaptation prior to various stress treatment. We found that heat or salt adapted KLB46 showed higher cell viability than non adapted upon heat stress at $60^{\circ}C$ for 20 min. When chloramphenicol was added during the adaptation process, heat tolerance was abolished. This result suggested that de novo protein synthesis was essential during adaptation.

• Journal of Sericultural and Entomological Science
• /
• v.42 no.1
• /
• pp.1-5
• /
• 2000

To investigate the possibility of crypreservation of mulberry seeds in liquid nitrogen(LN$_2$), characteristics of the seeds were examined after picking mulberry syncarps and drying-heat treatment. Storage in LN$_2$has the potential of providing indifinite preservation of valuable seed germplasm. Determining the tolerance of seeds among given cultivars to LN$_2$cooling and subsequent rewarming is the first step to establishing the feasibility of LN$_2$storage. Seeds of 4 mulberry varities were treated to LN$_2$(-196$\^{C}$) for 24 hours after drying heat treatment. Seed moisture content of Daeryukppong was the highest. As moisture content of mulberry seed was below 1%, storage in LN$_2$was safe. And drying heat treatment for 60 minutes was suitable to prevent decreased germination rate and germination vigor of seeds. The seeds of Cheongilppong were unsuibable to cryopreserve in LN$_2$for longterm storage.

• Journal of Welding and Joining
• /
• v.22 no.6
• /
• pp.30-35
• /
• 2004

Laser welding is obviously an attractive method to join small, thin parts such as small stainless steel tubes, but it is very sensitive to the joint clearance and tolerance, and this makes laser welding difficult to obtain consistent welding qualities over time. Recently, Plasma Augmented Laser Welding(PALW) is being developed to solve these problems. In this study, plasma arc welding(PAW) was introduced to join conventional V-grooved butt joint of thin stainless steel strips using single laser heat source in manufacturing small stainless steel tubes. The effect of the welding speed enhancement is investigated by the experiments. Effects of welding directions, distance between the heat sources and intensity of arc heat source on the optimal welding speed was investigated. Through this research, it was confirmed that PALW process has higher welding speed and robustness than laser welding process.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.38 no.3
• /
• pp.190-195
• /
• 2018

Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold ($4^{\circ}C$), salt (NaCl 200 mM) and heat ($42^{\circ}C$) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.31-31
• /
• 2022

Rice contains a wealth of genetic diversity, both within Oryza sativa and in related A-genome species. Decades of genetic research into this diversity have identified dozens of major genes contributing to a wide variety of important traits, including disease resistance, abiotic stress tolerance (drought, salinity, submergence, heat, cold etc.), grain quality, flowering date and maturity and plant architecture. Yet despite these opportunities, very few of the major genes and QTLs known have been successfully applied through rice breeding programs to produce sustained changes in farmer's fields. This presentation will briefly examine some of the factors limiting application of major genes in the mainstream breeding programs, and steps that have been taken to alleviate those limitations. As a result of these interventions, dozens of major genes that were previously unavailable to breeders are now being used confidently in the variety development process. Case studies will be discussed of genes critical for blast resistance worldwide, rice yellow mottle virus for Africa, and new validated QTLs for salinity tolerance.