• Journal of Biomedical and Translational Research
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• 제19권4호
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• pp.65-72
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• 2018

The purpose of this study was to investigate probiotic characteristics and immune enhancing effects of Bifidobacterium (B.) longum KBB1-26 and BIF-4, B. breve KBB5-22 isolated from human intestine for probiotic use in humans and animals. We measured acid, bile and heat tolerance, antimicrobial activity against pathogenic bacteria, Escherichia (E.) coli, Salmonella (S.) Enteritidis, Staphylococcus (S.) aureus, and Listeria (L.) monocytogenes. Immune enhancing effects of B. longum and B. breve were investigated by measuring nitric oxide (NO), nuclear factor ($NF-{\kappa}b$), $interleukin-1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6), interleukin-12 (IL-12) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) in RAW 264.7 cells or RAW BLUE cells. B. longum KBB1-26 was survived at pH 2.0. B. longum KBB1-26 and BIF-4, B. breve KBB5-22 also showed tolerance to 0.3% of oxgall bile salt. B. longum KBB1-26 was able to survive at $70^{\circ}C$ and $80^{\circ}C$ for 20 min. KBB1-26 showed the antimicrobial inhibition zone to pathogenic bacteria such as E. coli (12 mm), S. Enteritidis (14 mm), S. aureus (14 mm) and L. monocytogenes (41 mm). The production of NO ($4.5{\pm}0.00{\mu}M/mL$) and $IL-1{\beta}$ ($39.7{\pm}0.55pg/mL$) of KBB1-26 significantly higher than BIF-4 and KBB5-22, respectively. In addition, KBB1-26 and KBB5-22 induce the production of high level of $TNF-{\alpha}$ and IL-6 in macrophages. Collectively, B. longum KBB1-26 have acid, bile, heat tolerance, antimicrobial activity and immune enhancing effects. These results suggest that KBB1-26 can be used as probiotics for humans and animals.

• 원예과학기술지
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• 제32권2호
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• pp.142-148
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• 2014

Effect of silicon (Si) nutrition on growth and temperature stress tolerance of Nephrolepis exaltata 'Corditas' grown in a soilless substrate was examined. In vitro-grown acclimatized plantlets were transplanted into the pots containing a coir-based substrate. A nutrient solution containing 0, 50, or $100mg{\cdot}L^{-1}$ Si was supplied through a drip-irrigation system. After 5 months of cultiv ation, S i-treated and -untreated p lants were grown at 10, 25, or $40{\pm}1^{\circ}C$ under a 12 h photoperiod with $530{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and 60% RH. After 7 days, chlorophyll content and chlorophyll fluorescence parameters were measured. Silicon nutrition had a negative effect on growth characteristics of N. exaltata 'Corditas'. However, Si-treated plants had more tolerance to temperature stress than the control plants. The Fv/Fm value was not significantly different when the plants were exposed to $25^{\circ}C$. However, significant difference in Fv/Fm was recorded when plants were exposed to 10 or $40^{\circ}C$. Thus, Fv/Fm could be used as an indicator of low and high temperature tolerance in ferns. The present study also suggests that application of Si may be used to enhance temperature tolerance of ferns.

• Journal of Oral Medicine and Pain
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• 제32권4호
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• pp.413-419
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• 2007

다양한 종류의 정량적 통각검사들의 연관성을 알아보기 위하여 56 명의 측두하악장애 환자를 대상으로 측두근, 교근, 측두하악관절 부위, 그리고 경골근의 냉통각역치 (Cold Pain Threshold, CPT), 열통각역치 (Heat Pain Threshold, HPT), 열통증인내역치 (Heat Pain Tolerance Threshold, PTT), 압력통각역치 (Pressure Pain Threshold, PPT)를 측정하였으며, 각기 다른 통각 역치 간의 상관관계와 측정 부위 별 통각 역치 간의 상관 관계를 분석하였다. CPT, HPT, PTT를 포함한 온도통각역치의 성별간 차이는 나타나지 않았다. 그러나 PPT는 여성이 남성에 비하여 모든 부위에서 유의하게 낮은 역치를 나타내었다. CPT, HPT, PTT를 포함한 세 가지의 온도통각역치들은 모든 측정 부위에서 약정도에서 강정도 (mild to high)의 상관관계를 나타내었다 (r= 0.324-0.754, p<0.05). PPT 값은 각각의 온도통각역치와 통계적으로 유의한 상관관계를 나타내지 않았다. 모든 측정 부위의 통각역치값들은 서로간에 약정도에서 강정도 (mild to high)의 상관관계를 나타내었다 (r= 0.284-0.878, p<0.05). 측두하악장애 환자의 온도통각역치와 열통각인내역치 사이에는 유의한 상관관계가 존재하나 온도통각역치와 압력통각역치 간에는 상관관계가 나타나지 않는 것이 관찰되었으며, 각기 다른 부위에서 측정된 통각역치 간에는 비교적 높은 상관관계가 나타났다.

• Journal of Mechanical Science and Technology
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• 제18권10호
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• pp.1809-1818
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• 2004

The dependence of the ignition timing in an HCCI engine on intake temperature and pressure, equivalence ratio, and fuel species is investigated with a zero-dimensional model combined with a detailed chemical kinetics. The accuracy of the model is evaluated by comparing measured and computed results in a propane-fueled HCCI engine. It is shown that the peak pressure values are reproduced within 10% and ignition timing within 5$^{\circ}$ CA. The heat loss through the walls is found to affect significantly on the ignition timing for different inlet conditions. It is also shown that for the propane-fueled engine, the tolerance in intake temperatures is 20-25K and the tolerance in intake pressure is about 1 bar for stable operation without misfire or too early ignition. Comparison of propane and heptane fuels indicates that the tendency to misfire when heptane is employed as the fuel is less than that when propane is employed with the same wall temperature conditions. However, the heptane-fueled engine may have a lower compression ratio to avoid too early ignition and hence lower efficiency. For the selected set of engine parameters, stable operations might be achieved for the heptane-fueled engine with twice as much tolerance in intake temperatures as for the propane-fueled engine.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.400-407
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• 2010

Zoysiagrass (Zoysia japonica Steud.), also called Korean or Japanese lawngrass, is the most popular warm-season turfgrass in Korea and is widely used for home lawns, parks, roadsides, golf courses and athletic fields. Its use is rapidly expanding in Korea and the other countries, due to its excellent characteristics which include tolerance to heat, drought and salinity. As the utilization area of this turfgrass increases, there is an increase in the demand for improved cultivars with disease and insect tolerance or with herbicide-tolerance or with extended greening periods. Conventional breeding methods have been used to improve the traits described above with limited success. However, with the advances in biotechnology, genetic transformation can be utilized for turfgrass improvement. In this paper, we review recent progress in biotechnological improvement of zoysiagrass and discuss future molecular breeding of this species.

• KSBB Journal
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• 제29권2호
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• pp.81-86
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• 2014

Lactobacilli, the dominant species of microorganisms in the vaginal flora of healthy women, play important roles to prevent bacterial vaginosis and other sexually transmitted diseases. In this study, we carried out studies on stress adaptation prior to various stress treatment. We found that heat or salt adapted KLB46 showed higher cell viability than non adapted upon heat stress at $60^{\circ}C$ for 20 min. When chloramphenicol was added during the adaptation process, heat tolerance was abolished. This result suggested that de novo protein synthesis was essential during adaptation.

• 한국잠사곤충학회지
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• 제42권1호
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• pp.1-5
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• 2000

To investigate the possibility of crypreservation of mulberry seeds in liquid nitrogen(LN$_2$), characteristics of the seeds were examined after picking mulberry syncarps and drying-heat treatment. Storage in LN$_2$has the potential of providing indifinite preservation of valuable seed germplasm. Determining the tolerance of seeds among given cultivars to LN$_2$cooling and subsequent rewarming is the first step to establishing the feasibility of LN$_2$storage. Seeds of 4 mulberry varities were treated to LN$_2$(-196$\^{C}$) for 24 hours after drying heat treatment. Seed moisture content of Daeryukppong was the highest. As moisture content of mulberry seed was below 1%, storage in LN$_2$was safe. And drying heat treatment for 60 minutes was suitable to prevent decreased germination rate and germination vigor of seeds. The seeds of Cheongilppong were unsuibable to cryopreserve in LN$_2$for longterm storage.

• Journal of Welding and Joining
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• 제22권6호
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• pp.30-35
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• 2004

Laser welding is obviously an attractive method to join small, thin parts such as small stainless steel tubes, but it is very sensitive to the joint clearance and tolerance, and this makes laser welding difficult to obtain consistent welding qualities over time. Recently, Plasma Augmented Laser Welding(PALW) is being developed to solve these problems. In this study, plasma arc welding(PAW) was introduced to join conventional V-grooved butt joint of thin stainless steel strips using single laser heat source in manufacturing small stainless steel tubes. The effect of the welding speed enhancement is investigated by the experiments. Effects of welding directions, distance between the heat sources and intensity of arc heat source on the optimal welding speed was investigated. Through this research, it was confirmed that PALW process has higher welding speed and robustness than laser welding process.

• 한국초지조사료학회지
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• 제38권3호
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• pp.190-195
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• 2018

Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold ($4^{\circ}C$), salt (NaCl 200 mM) and heat ($42^{\circ}C$) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.31-31
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• 2022

Rice contains a wealth of genetic diversity, both within Oryza sativa and in related A-genome species. Decades of genetic research into this diversity have identified dozens of major genes contributing to a wide variety of important traits, including disease resistance, abiotic stress tolerance (drought, salinity, submergence, heat, cold etc.), grain quality, flowering date and maturity and plant architecture. Yet despite these opportunities, very few of the major genes and QTLs known have been successfully applied through rice breeding programs to produce sustained changes in farmer's fields. This presentation will briefly examine some of the factors limiting application of major genes in the mainstream breeding programs, and steps that have been taken to alleviate those limitations. As a result of these interventions, dozens of major genes that were previously unavailable to breeders are now being used confidently in the variety development process. Case studies will be discussed of genes critical for blast resistance worldwide, rice yellow mottle virus for Africa, and new validated QTLs for salinity tolerance.