• Journal of the Korean Society of Manufacturing Process Engineers
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• 제10권2호
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• pp.84-89
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• 2011

This paper studied the carburizing of chromium molybdenum steel which the heat treatment effect of gear geometric tolerance, OPD, Runout, the surface hardness, the maximum hardness, the core hardness and the bending fatigue strength were investigated. Firstly, the deformation is observed, and the results of circularity, squareness, OPD and Runout of SCM822, SCM425, and SCM415 are obtained in order. Secondly, in order to investigate the gear hardness, the surface hardness, the maximum hardness and the core hardness of SCM822, SCM425, and SCM415 are obtained; and the surface hardness of SCM822 is about 10% higher than SCM415's, and about 3% higher than SCM425's. Thirdly, the fatigue strength of SCM822 is about 10% higher than SCM415's, and about 7% higher than SCM425's in the fatigue test results. At last, for the purpose of the minimum deformation of heat treatment, and also the improvement of fatigue strength, the best gear material is SCM822 in this test.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1119-1125
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• 2024

Phytase increases the availability of phosphate and trace elements by hydrolyzing the phosphomonoester bond in phytate present in animal feed. It is also an important enzyme from an environmental perspective because it not only promotes the growth of livestocks but also prevents phosphorus contamination released into the environment. Here we present a novel phytase derived from Turicimonas muris, TmPhy, which has distinctive structure and properties compared to other previously known phytases. TmPhy gene expressed in the Pichia system was confirmed to be 41 kDa in size and was used in purified form to evaluate optimal conditions for maximum activity. TmPhy has a dual optimum pH at pH3 and pH6.8 and exhibited the highest activity at 70℃. However, the heat tolerance of the wildtype was not satisfactory for feed application. Therefore, random mutation, disulfide bond introduction, and N-terminal mutation were performed to improve the thermostability of the TmPhy. Random mutation resulted in TmPhyM with about 45% improvement in stability at 60℃. Through further improvements, a total of three mutants were screened and their heat tolerance was evaluated. As a result, we obtained TmPhyMD1 with 46.5% residual activity, TmPhyMD2 with 74.1%, and TmPhyMD3 with 66.8% at 80℃ heat treatment without significant loss of or with increased activity.

• Journal of the Korean Society of Clothing and Textiles
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• 제36권3호
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• pp.259-268
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• 2012

This study suggests quantitative guidelines for inside clothing temperatures to improve the heat tolerance of 20's females in summer. First, the inside clothing temperatures ($T_{cl}$) of each subject was measured in daily use. The subjects were asked to record subjective thermal sensations, clothing items worn, clothing weight, and activities during an experiment designed to determine the comfort zone of $T_{cl}$. In a thermally neutral state, the comfort zone of $T_{cl}$ was decided on a mean value $T_{cl}{\pm}1{\sigma}$. Second, the subjects were asked to wear clothing that would enable them to feel 'slightly warm but still comfortable'. The rest of the processes were the same as previous steps that were designed to understand the way and degree of clothing control. The comfort zone of $T_{cl}$ was decided in the same manner as the previous step. The two comfort zones were combined and named the combined comfort zone of the definitive comfort zone. The results were as follows: 1. Thermally comfortable $T_{cl}$, Hcl were $34.0{\pm}1.1^{\circ}C$, $40{\pm}9%%RH$ and the thermally comfortable ambient climate was $25.0{\pm}1.6^{\circ}C$, $53{\pm}7%$RH. 2. When subjects were asked to wear 'slightly warm but still comfortable', there were difference in thermally comfortable $T_{cl}$, clothing weight and clothing layer by subject. 3. In this study, the optimal $T_{cl}$ was decided on the mid-point of the definitive comfort zone of $T_{cl}$.

• Molecules and Cells
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• 제39권6호
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• pp.477-483
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• 2016

Heat shock factors (Hsfs) are central regulators of abiotic stress responses, especially heat stress responses, in plants. In the current study, we characterized the activity of the Hsf gene HsfA3 in Arabidopsis under oxidative stress conditions. HsfA3 transcription in seedlings was induced by reactive oxygen species (ROS), exogenous hydrogen peroxide ($H_2O_2$), and an endogenous $H_2O_2$ propagator, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB). HsfA3-overexpressing transgenic plants exhibited increased oxidative stress tolerance compared to untransformed wild-type plants (WT), as revealed by changes in fresh weight, chlorophyll fluorescence, and ion leakage under light conditions. The expression of several genes encoding galactinol synthase (GolS), a key enzyme in the biosynthesis of raffinose family oligosaccharides (RFOs), which function as antioxidants in plant cells, was induced in HsfA3 overexpressors. In addition, galactinol levels were higher in HsfA3 overexpressors than in WT under unstressed conditions. In transient transactivation assays using Arabidopsis leaf protoplasts, HsfA3 activated the transcription of a reporter gene driven by the GolS1 or GolS2 promoter. Electrophoretic mobility shift assays showed that GolS1 and GolS2 are directly regulated by HsfA3. Taken together, these findings provide evidence that GolS1 and GolS2 are directly regulated by HsfA3 and that GolS enzymes play an important role in improving oxidative stress tolerance by increasing galactinol biosynthesis in Arabidopsis.

• KIEAE Journal
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• 제8권5호
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• pp.49-54
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• 2008

The experimental of temperature, humidity and velocity was taken from the underground pit which utilized the system of ground heat source quite similar to the cool-pit system. Also, through CFD analysis, one could review the effectiveness of analysis of future alternatives. Furthermore, the temperature range of mock up cool-pit system was analyzed by inputting the weather data of annual average soil temperature provided by KMA(Korea Meteorological Administration) into the fluid simulation of anticipated heat distribution. Firstly, the difference between the temperature of air exhaust of the pit or the temperature of air supply of the compressor room and the experimental data for the month of May from the CFD analysis came out to be $0.6^{\circ}C$ and $0.9^{\circ}C$ respectively with tolerance of 3.1% and 4.7%. Secondly, the difference between the temperature of air exhaust of the Pit or the temperature of air supply of the compressor room and the experimental data for the month of July from the CFD analysis came out to be $0.8^{\circ}C$ and $1.1^{\circ}C$ respectively with tolerance of 3.3% and 4.5%. Thirdly, for the month of May, the difference between the experimental data taken for the air exhaust of the Pit or the air supply of the compressor room and soil temperature provided by KMA for monthly and yearly average temperature of Jeonju region came out be $1.9^{\circ}C$ and $1.8^{\circ}C$ respectively with tolerance of 10.7% and 9.8%. Fourthly, for the month of July, the difference between the experimental data taken for the air exhaust of the Pit or the air supply of the compressor room and soil temperature provided by KMA for monthly and yearly average temperature of Jeonju region came out be $1.1^{\circ}C$ and $1.4^{\circ}C$ respectively with tolerance of 4.5% and 5.8%. The result of above experiments allowed us to establish CFD model set up as a verification tool that is based on experimental data collected within the Pit area. Also, one could confirm the possibility to apply weather data of soil temperature provided by KMA in order to anticipate proper value for CFD analysis.

• Food Science of Animal Resources
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• 제30권3호
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• pp.479-486
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• 2010

Tolerance against heat, sanitizers, and antibiotics of 112 Cronobacter isolates classified by desiccation was determined to permit effective biocontrol in powdered foods. The isolates were classified into three groups: dry-tolerant (n=37), dry-sensitive (n=7), and dry-intermediate (n=68). The strains that were highly tolerant to drying also showed high heat tolerance that they seemed to have high tolerance to heat after dry stress in powdered foods like infant formula. Sodium hypochlorite and benzalkonium chloride concentrations necessary to achieve a 5-log reduction in viable counts (CFU/mL) were 15-25 ppm and 5-15 ppm, respectively. However, there was little difference of the efficacy of these sanitizers between dry-sensitive and -tolerant strains for planktonic cells suspended in 3% albumin. The minimal inhibition concentration (MIC) of $\beta$-lactam ampicillin was 64-128 ppm for 90% of the strains. The isolates were consistently sensitive to kanamycin and naldixic acid (MIC=4 ppm). Dry-tolerant strains displayed more antibiotic resistance than dry-sensitive strains. The results indicate that dry-tolerant Cronobacter isolates often possess heat and antibiotic resistance, indicated the need for potent sterilization treatments of powdered foods.

• Korean journal of applied entomology
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• 제55권1호
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• pp.17-26
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• 2016

Climate change has been regarded as one of main factors to change Korean insect pest fauna. Especially, a global warming model predicts to expand habitat for insect pests originated from tropical or subtropical regions. Two insect pests, the beet armyworm (Spodoptera exigua) and the diamondback moth (Plutella xylostella), are known to overwinter in some greenhouse conditions without diapause induction in Korea. There was a clear difference between these two insects in seasonal occurrence. P. xylostella occurred only at early spring and fall seasons, but did not occur during summer. In contrast, S. exigua maintained their occurrence from late spring to fall seasons. This study set up a hypothesis that the difference in the seasonal occurrence may be resulted from variation in susceptibility to high temperature. To test the hypothesis, heat tolerance was compared between these two insects. Exposure to $42^{\circ}C$ for 40 min killed 100% individuals of P. xylostella larvae. However, most larvae of S. exigua survived in response to $42^{\circ}C$ even for 80 min. Heat tolerance varied among developmental stages in both insects. Highest tolerant stages were $4^{th}$ instar larvae and adults for P. xylostella, but $1^{st}$ instar larvae for S. exigua. Pre-exposure to $37^{\circ}C$ for 30 min significantly increased heat tolerance in both insects. Induction of heat tolerance accompanied with significant increase of glycerol contents in the hemolymph in both insects and up-regulation of three heat shock protein expressions in S. exigua. These results suggest that the differential susceptibility to high temperature explains the disappearance of P. xylostella during summer, at which S. exigua maintains its occurrence.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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• pp.131-131
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• 2005

• Journal of the Korean Society for Precision Engineering
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• 제27권11호
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• pp.84-91
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• 2010

Heat treatment with carburized quenching process is widely used for automotive helical gear to improve its surface properties of hardness and strength. However, the gear can be deformed with the process over the allowable tolerance, which possibly makes noise, vibration and heat problems in operation. In this study, deformation of helical gear during heat treatment of carburized quenching was analyzed with a numerical method, incorporating coupled calculations of thermal conduction, carbon diffusion, phase transformation and thermal stresses. With the analysis, the effect of coolant temperature in quenching on the deformation was investigated. The result of the analysis revealed that the higher the coolant temperature became, the more change of helix angle and the more compressive stresses in the surface generated, because of delayed generation of martensite in the part.

• Journal of Life Science
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• 제29권8호
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• pp.916-921
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• 2019

In this study, we constructed a biological system that exhibited thermotolerance, ethanol tolerance, and increased ethanol productivity using a random mutagenesis method. We attempted to isolate a thermotolerant mutant using proofreading-deficient DNA polymerase ${\delta}$ and ${\varepsilon}$ encoded by the pol3 and pol2 genes, respectively, in Saccharomyces cerevisiae. To obtain mutants that could grow at high temperatures ($38^{\circ}C$ and $40^{\circ}C$), random mutagenesis of AMY410 (pol2-4) and AMY126 (pol3-01) strains was induced. The parental strains (AMY410 and AMY126) grew poorly at temperatures higher than $38^{\circ}C$. By stepwise elevation of the incubation temperature, AMY410-Ht (heat tolerance) and AMY126-Ht strains that proliferated at $40^{\circ}C$ were obtained. These strains were further incubated in medium containing 6% and 8% ethanol and then AMY410-HEt (heat and ethanol tolerance) and AMY126-HEt strain with ethanol tolerance at an 8% ethanol concentration was obtained. The AMY126-HEt strain grew even at an ethanol concentration of 10%. Furthermore, following the addition of high concentrations of glucose (5% and 10%), an AMY126-HEt3 strain with increased ethanol productivity was isolated. This strain produced 24.7 g/l of ethanol (95% theoretical conversion yield) from 50 g/l of glucose. The findings demonstrate that a new biological system (yeast strain) showing various phenotypes can be easily and efficiently bred by random mutagenesis of a proofreading- deficient mutant.