• BMB Reports
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• v.39 no.5
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• pp.636-641
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• 2006

Our previous studies indicated that native carbonic anhydrase does not interact with hydrophobic adsorbents and that it acquires this ability upon denaturation. In the present study, an apo form of the enzyme was prepared by removal of zinc and a comparative study was performed on some characteristic features of the apo and native forms by far- and near-UV circular dichroism (CD), intrinsic fluorescent spectroscopy, 1-anilino naphthalene-8-sulfonate (ANS) binding, fluorescence quenching by acrylamide, and Tm measurement. Results indicate that protein flexibility is enhanced and the hydrophobic sites become more exposed upon conversion to the apo form. Accordingly, the apo structure showed a greater affinity for interaction with hydrophobic adsorbents as compared with the native structure. As observed for the native enzyme, heat denaturation of the apo form promoted interaction with alkyl residues present on the adsorbents and, by cooling followed by addition of zinc, catalytically-active immobilized preparations were obtained.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.430.1-430.1
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• 2014

According to advanced nanotechnology in the field of biomedical engineering, many studies of the interaction between topography of surfaces and cellular responses have been focused on nanostructure. In order to investigate this interaction, it is essential to make well-controlled nanostructures. Electron beam lithography (EBL) have been considered the most typical processes to fabricate and control nano-scale patterns. In this work, $TiO_2$ nanowire array was fabricated with hybrid process (top-down and bottom-up processes). Nanodot arrays were patterned on the substrate by EBL process (top-down). In order to control the spacing between nanodots, we optimized the EBL process using Poly(methyl methacrylate) (PMMA) as an electron beam resist. Metal lift-off was used to transfer the spacing-controlled nanodots as a seed pattern of $TiO_2$ nanowire array. Au or Sn nanodots which play an important role for catalyst using Vapor-Liquid-Solid (VLS) method were patterned on the substrate through the lift-off process. Then, the sample was placed in the tube furnace and heated at the synthesis temperature. After heat treatment, $TiO_2$ nanowire array was fabricated from the nanodots through VLS method (bottom-up). These results of spacing-controlled nanowire arrays will be used to study the interaction between nanostructures and cellular responses in our next steps.

• Journal of the Korean GEO-environmental Society
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• v.10 no.6
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• pp.125-133
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• 2009

Rock fracturing technique through fluid injection into the wellbore has been widely used to extract geothermal heat and to enhance oil and gas production. Single fracture formation is ideal for the production. However, it is very difficult to form single fracture formation. Instead, the formation of segmented fracture is a common phenomenon. Therefore, design parameters are expected to be different from those of single fracture because of mechanical interaction between segmented fractures. In this paper, design parameters such as length, aperture, and net pressure are evaluated by using model of segmented fracture in which numerical technique is incorporated to consider mechanical interaction between segments. Results show that the existence of fracture segmentation affects design parameters in fracturing treatment in rock by fluid injection.

• Nuclear Engineering and Technology
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• v.45 no.7
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• pp.839-846
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• 2013

This paper describes a finite element model of the microstructure of dispersion type nuclear fuels, which can be used to determine the effective thermal conductivity of the fuels during irradiation. The model simulates a representative region of the fuel as a prism shaped unit cell made of brick elements. The elements within the unit cell are assigned material properties of either the fuel or the matrix depending on position, in such a way as to represent randomly distributed fuel particles with a size distribution similar to that of the as manufactured fuel. By applying an appropriate heat flux across the unit cell it is possible to determine the effective thermal conductivity of the unit cell as a function of the volume fraction of the fuel particles. The presence of a fuel/matrix interaction layer is simulated by the addition of a third set of material properties that are assigned to the finite elements that surround each fuel particle. In this way the effective thermal conductivity of the material may also be determined as a function of the volume fraction of the interaction layer. Work is on going to add fission gas bubbles in the fuel as a fourth phase to the model.

• Journal of the Korean Society of Propulsion Engineers
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• v.17 no.2
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• pp.1-8
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• 2013

This study presents relations between the time lag and interaction index of the impinging-jet injectors using the time lag model in a model chamber. The response of the flame is analyzed to artificial perturbation with 5% amplitude of oxidizer speed at a resonance frequency. At the mixing point of fuel and oxidizer, which determines the characteristic length, the relationship between velocity perturbation and heat release rate is quantified by combustion parameters of interaction index and time lag. In this method, time lag or delay is calculated by the characteristic length and the average velocity obtained from numerical results. The tendency that the time delay decreases with axial jet velocity has been observed.

• Nuclear Engineering and Technology
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• v.51 no.4
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• pp.1008-1016
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• 2019

In the SG (steam generator) of PWR (pressurized water reactor) for a nuclear plant, hundreds of U-shaped tubes are used for the heat exchanger system. They interact with primary pressurized cooling water flow, generating flow-induced vibration in the secondary flow region. A simplified U-tube model is proposed in this study to apply for experiment and its counterpart computation. Using the commercial code, ANSYS-CFX, we first verified the Moody chart, comparing the straight pipe theory with the results derived from CFD (computational fluid dynamics) analysis. Considering the virtual mass of fluid, we computed the major modes with the low natural frequencies through the comparison with impact hammer test, and then investigated the effect of pump flow in the frequency domain using FFT (fast Fourier transform) analysis of the experimental data. Using two-way fluid-structure interaction module in the CFD code, we studied the influence on mean flow rate to generate the displacement data. A feasible CFD method has been setup in this research that could be applied potentially in the field of nuclear thermal-hydraulics.

• Nuclear Engineering and Technology
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• v.53 no.3
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• pp.1020-1028
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• 2021

The nuclear reactor coolant pump transferring heat energy inherently brings with it the unsteady flow and inevitably threatens to the safe operation of the pump unit, especially with the pressure pulsation induced by the rotor-stator interaction. In this paper, the characteristics of pressure pulsation of the diffuser in a nuclear reactor coolant pump were investigated by the numerical simulation with experimental validation. Pressure pulsation signals measured synchronously from sensors mounted on the radial diffuser of a model pump were analyzed via Welch's method. Frequency components induced by the rotor-stator interaction can be revealed by the diameter mode analysis method. The pressure pulsation of the diffuser is dominated by the blade passing frequency and its harmonics, which are free from the effect of flow rate and rotational speed while the corresponding amplitudes are easily affected by different operational conditions and measuring positions. The non-uniformity is much more affected by the rotational speed than the flow rate. This research is helpful for further work to reduce the pressure pulsation for the reactor coolant pump.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.2
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• pp.143-149
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• 2008

The present study is mainly motivated to investigate the vaporization, auto-ignition and spray combustion processes in DI diesel engine using DME and n-heptane. In order to realistically simulate the dimethyl ether (DME) spray dynamics and vaporization characteristics in high-pressure and high-temperature environment, the high-pressure vaporization model has been utilized. The interaction between chemistry and turbulence is treated by employing the Representative Interaction Flamelet (RIF) model. The detailed chemistry of 336 elementary steps and 78 chemical species is used for the DME/air reaction. Based on numerical results, the detailed discussion has been made for the distinctly different combustion characteristics of DME diesel engine in term of vaporization, ignition delay, pollutant formation, and heat release rate.

• 한국연소학회:학술대회논문집
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• 2012.11a
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• pp.5-7
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• 2012

This study presents relations between the time lag and interaction index of the impinging-jet injectors using time lag model in a model chamber. To analyze the response of the flame, 5% amplitude of oxidizer velocity is artificially perturbed at a resonance frequency. At the mixing point of fuel and oxidizer, which determines the characteristic length, the relationship between velocity perturbation and heat release rate is quantified by combustion parameters of interaction index and time lag. As the improved method to apply the time-lag, the method using the average velocity obtained from numerical results is suggested.

• 한국전산유체공학회:학술대회논문집
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• 2010.05a
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• pp.362-364
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• 2010

Shock wave interaction with droplet-gas medium is investigated in this paper. In the present computation, the shock wave is initially started in a pure gas and reflected from the wedge to interact with the droplet-ridden gas flows. We used the compressible two-fluid two-phase model that is solved by the two-fluid version of the HLL scheme. The interfacial drag force and heat transfer were included to model the interaction between continuous and dispersed phases. The parametric effect of void fraction on the shock wave reflection in the two-phase media was investigated.