• The Korean Journal of Physiology and Pharmacology
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• 제21권5호
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• pp.531-546
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• 2017

Activation of Toll-like receptor-4 (TLR-4) in articular chondrocytes increases the catabolic compartment and leads to matrix degradation during the development of osteoarthritis. In this study, we determined the proteomic and genomic alterations in human chondrocytes during lipopolysaccharide (LPS)-induced inflammation to elucidate the underlying mechanisms and consequences of TLR-4 activation. Human chondrocytes were cultured with LPS for 12, 24, and 36 h to induce TLR-4 activation. The TLR-4-induced inflammatory response was confirmed by real-time PCR analysis of increased interleukin-1 beta ($IL-1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor alpha ($TNF-{\alpha}$) expression levels. In TLR-4-activated chondrocytes, proteomic changes were determined by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-mass spectroscopy analysis, and genomic changes were determined by microarray and gene ontology analyses. Proteomics analysis identified 26 proteins with significantly altered expression levels; these proteins were related to the cytoskeleton and oxidative stress responses. Gene ontology analysis indicated that LPS treatment altered specific functional pathways including 'chemotaxis', 'hematopoietic organ development', 'positive regulation of cell proliferation', and 'regulation of cytokine biosynthetic process'. Nine of the 26 identified proteins displayed the same increased expression patterns in both proteomics and genomics analyses. Western blot analysis confirmed the LPS-induced increases in expression levels of lamin A/C and annexins 4/5/6. In conclusion, this study identified the time-dependent genomic, proteomic, and functional pathway alterations that occur in chondrocytes during LPS-induced TLR-4 activation. These results provide valuable new insights into the underlying mechanisms that control the development and progression of osteoarthritis.

• Perspectives in Nursing Science
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• 제2권1호
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• pp.37-47
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• 2005

Biobehavioral nursing research is focused on generating knowledge that examines relations among biological, behavioral, and social dimensions of health to improve outcomes. In this paper we review the findings of a biobehavioral nursing study of individuals with fibromyalgia (FM) that was framed from the perspective of an individual human response model, the FM literature, and our previous studies in midlife women. We were particularly interested in the studying the role of 'arousal' secondary to pain or to dysregulated hypothalamic-pituitary-adrenal (HPA) axis hormones during sleep and the impact on symptom expression. Unexpectedly, we did not find evidence of, arousal' or abnormal amounts of HPA axis hormones but we did find reduced amounts of growth hormone (GH) and prolactin (PRL) and of sleep spindle activity, a biomarker of sleep maintenance. We discuss these new findings and how our thinking was re-shaped to better understand the role that disturbed sleep plays in symptom expression in FM. It is argued that disturbed sleep maintenance mechanisms coupled with dysregulated somatotrophic-growth hormone axis and sleep-related PRL render individuals vulnerable to the development of or exacerbations of FM symptoms.

• Child Health Nursing Research
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• 제7권1호
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• pp.5-20
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• 2001

The purpose of this study was to analyze and identify the higher-grade primary school children's needs for sexual education. We got the data with open question from 481 children from March to July 1999. We analyzed them according to the framework suggested by the Sex Information and Education Council of USA. The framework involves the six domains such as human development, relationship, personal skills, sexual behavior, sexual health, and society & culture. In the analysis of need for sexual education according to domain, need for human development was highest (79.2 ％), after that followed society & culture (8.3 ％), sexual health (5.8％), sexual behavior (3.6％), and relationships (3.1％). There was no need for personal skills. By topics, there were needs for puberty (38.0％), reproduction (32.5％), gender roles (6.0％), body image (4.9％), sexual abuse (2.4％) and reproductive health (2.4％). Girls and boys all mostly wanted to know human development 41.0 ％ and 38.2 ％ each. Society & culture (3.4 ％), sexual behaviour (2.7％), sexual health (2.6％), relationship (2.4％) were domains selected by boys. Girls wanted to know society & culture (4.9％) and 'sexual health (3.2％). Comparing by grade, fourth, fifth and sixth grade children are commonly interested in development, society & culture. Fourth grade children scarcely show attention on the relationship, sexual behaviour and sexual health; meanwhile sixth grade children would like to know sexual behaviour and sexual health. When their grade became higher, they would like to know more precise, concrete and deep information. We suggest that the textbook/guidebook for sexual health for the higher-grade primary school children should be developed considering the sex and grade.

• Safety and Health at Work
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• 제6권3호
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• pp.184-191
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• 2015

Chemical mutagenicity is a major hazard that is important to workers' health. Despite the use of large amounts of allyl chloride, the available mutagenicity data for this chemical remains controversial. To clarify the mutagenicity of allyl chloride and because a micronucleus (MN) test had not yet been conducted, we screened for MN induction by using male ICR mice bone marrow cells. The test results indicated that this chemical is not mutagenic under the test conditions. In this paper, the regulatory test battery and several assay combinations used to determine the genotoxic potential of chemicals in the workplace have been described. Further application of these assays may prove useful in future development strategies of hazard evaluations of industrial chemicals. This study also should help to improve the testing of this chemical by commonly used mutagenicity testing methods and investigations on the underlying mechanisms and could be applicable for workers' health.

• Journal of Preventive Medicine and Public Health
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• 제50권2호
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• pp.91-99
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• 2017

Objectives: As in many low-income and middle-income countries, out-of-pocket (OOP) payments by patients or their families are a key healthcare financing mechanism in Bangladesh that leads to economic burdens for households. The objective of this study was to identify whether and to what extent socioeconomic, demographic, and behavioral factors of the population had an impact on OOP expenditures in Bangladesh. Methods: A total of 12 400 patients who had paid to receive any type of healthcare services within the previous 30 days were analyzed from the Bangladesh Household Income and Expenditure Survey data, 2010. We employed regression analysis for identify factors influencing OOP health expenditures using the ordinary least square method. Results: The mean total OOP healthcare expenditures was US dollar (USD) 27.66; while, the cost of medicines (USD 16.98) was the highest cost driver (61% of total OOP healthcare expenditure). In addition, this study identified age, sex, marital status, place of residence, and family wealth as significant factors associated with higher OOP healthcare expenditures. In contrary, unemployment and not receiving financial social benefits were inversely associated with OOP expenditures. Conclusions: The findings of this study can help decision-makers by clarifying the determinants of OOP, discussing the mechanisms driving these determinants, and there by underscoring the need to develop policy options for building stronger financial protection mechanisms. The government should consider devoting more resources to providing free or subsidized care. In parallel with government action, the development of other prudential and sustainable risk-pooling mechanisms may help attract enthusiastic subscribers to community-based health insurance schemes.

• Child Health Nursing Research
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• 제28권2호
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• pp.112-123
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• 2022

Purpose: This study aimed to develop and evaluate a mobile application focusing on developmental support care for infants born prematurely. Methods: An application was developed using the analysis, design, development, implementation, and evaluation model. In the analysis phase, previous research was evaluated through big data text-mining and a literature review. In the design phase, the preliminary content of the application was designed, and the content validity and comprehension were verified. A hybrid application was developed and used by eight experts and ten users, who evaluated the layout of the mobile application and their satisfaction with it. Results: The content of the designed application comprised a diary, customized information, developmental play, and community. The mean scores for layout were 3.73±0.47 and 3.43±0.68 out of 4 points among the experts and users, respectively. Users' mean satisfaction score was 3.70±0.70 out of 5 points. Conclusion: The information provided by the mobile application was evaluated as consistent and systematic. The application was also found to be satisfactory by infants' parents. The mobile application developed through this study is expected to be effective in supporting the development of children born prematurely.

• 부모자녀건강학회지
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• 제4권2호
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• pp.43-55
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• 2002

Relationship between parent and children is basic relation and initial relationship. It is essential research to explore relationship between parent's role satisfaction and children's health promotion in rapidly changing social situation influence parenting attitude. The purpose of this research to explore first mother's parenting belief, communication, filial piety by indepth interview and literature review. Method of research is literature review (books and articles) and Web site researching (home page) and contacted two mothers to do individual interview. Data was collected and analysed according to the objectives. The result of this study as follows. 1) It was problem that is child rearing and child education due to working parents. 2) It was discussed parent's belief regarding child rearing. The belief was influence by culture, social economic status, family friend and internet, advise of professionals. The subjects of this study was influenced by family, friend and internet Web site. 3) Parent-child communication was reviewed and discussed about influence of masmedia and early education system. Children's level of language development was rapidly improved by role play and read the book with mother. 4) It was discussed concept of filial piety. Parent was not expected filial piety from their children. Parent thought that they already received filial piety from their children, because children gave them pleasure and happiness during growing process. Parents want their children become mature person and adapt to informative changing society. Finally, it is needed further interdisciplinary study for parent-child health promotion and growth and development in rapidly changing society.

• 보건교육건강증진학회지
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• 제30권5호
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• pp.113-124
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• 2013

Objectives: The objective of this study was developing a community-based HIV prevention program to enhance the safe sexual behavior among rural Ethiopian female youth. Methods: A community-based HIV prevention program was developed using the Cox's Interaction Model of Client Health Behavior. The program development was carried out in four phases using a mixed research method: need-assessment which consisted of three steps (secondary-data analysis, key-informant interview, and focused-group interview); identification of preliminary program contents; expert's review of the program contents for the validity and cultural acceptability of the program; and refinement of the proposed program contents. Results: The HIV prevention program developed in this study consisted of three modules; the first module was for enhancing the youth's self-esteem, the second was for providing the youth with information regarding HIV/AIDS and safe sexual behavior, and the third was for improving the youth's communication skills and refusing skills. Conclusions: The need assessment and expert's review was very effective way to reflect sociocultural factors of rural Ethiopia for developing HIV prevention program for female youth. Further research is desirable for verifying the effectiveness of the developedprogram.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7011-7014
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• 2014

The polycystic ovary syndrome is the most common endocrinological disorder of reproductive age women with a prevalence of 5 to 8 %. The most common diagnostic criteria used for polycystic ovary syndrome are oligo- or an-ovulation, clinical and/ or biochemical signs of hyperandrogenism and polycystic ovaries. Hyperandrogenism results in increased estrogen levels and lack of cyclic progesterone due to anovulation and persistent stimulation of the endometrium may lead to endometrial hyperplasia or adenocarcinoma development. In this mini review, we aimed to evaluate the possible relationship between polycystic ovary syndrome and endometrial cancer.

• 보건교육건강증진학회지
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• 제13권1호
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• pp.45-51
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• 1996

The Korea Information Infrastructure aims at the construction of an advanced national information infrastructure, consisting of communication networks, computers, databases and multimedia terminals. In the emerging information society, a well-established national information network plays a crucial role in enhancing economic efficiency and creating national wealth. Till 2015, government plans to construct an Information Superhighway Network and to provide a telecommunication service for speedy transmission of multi-media typed information and development of various applied programs, which help government's commitment to establish nationwide infrastructure to perform a leading role as a high level information society in the 21st century. In the field of health education, the research monograph contains three main parts: health education and information, acceptability of health education; development of health education management information system. In the most remote areas, it can bring high-quality health care where none is now available. In global health care, it can enhance and standardize the quality of medical care throughout the world. Before enlarging the establishment of the health education network system, the issues from this study should be considered to improve the health status through the introduction of information technology and applications in health care.