• KSBB Journal
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• v.13 no.1
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• pp.83-89
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• 1998

Using a cellulose macroporous microcarrier, HeLa cells were cultivated in 100mL spinner flask(Bellco Co., USA) and confluent cell laden microcarriers were subcultured by bead-to-bead cell transfer method. In macroporous mcirocarrier-HeLa system viable suspended cells played an important role in bead-to-bead cell transfer and that could be increased by use of RPMI-1640, a calcium-ion-reduced-media and high speed agitation. Successful bead-to-bead cell transfers were performed continuously three time in spinner flask. We applied this technique to produce recombinant Vaccinia virus which express $\beta$-galactosidase. Recombinant protein yield of bead-to-bead transferred culture was comparable to conventional microcarrier cultures that were inoculated by cells detached from T-flask. Although trypsinization is a useful method for subculturing microcarriers in some cases, that process adds quality control problem and handling steps to large scale cell production. There fore, bead-to-bead cell transfer technique offers another convenient and efficient scale-up method for continuous microcarrier cultures.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2006.11a
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• pp.216-220
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• 2006

In this study, it was investigated that the characteristics of startup and compatibility using several type hot and cold gases. The characteristics of starting LRE by pyro starter was compared with that by a He spinner. The compatibility of pyre gas, a gaseous He, H2+N2 mixture gas, and air was investigated by a simple 1D turbine analysis considered the properties of each gases and turbine efficiency. Most of them were compatible to start up the LRE but air was properly used only when the turbine was low power mode.

• Aerospace Engineering and Technology
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• v.7 no.2
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• pp.170-175
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• 2008

In this study, it was investigated that the characteristics of startup and compatibility using several type hot and cold gases. The characteristics of starting LRE by pyro starter was compared with that by a Helium spinner. The compatibility of pyro gas, a gaseous Helium, Hydrogen+Nitrogen mixture gas, and air was investigated by a simple 1D turbine analysis considered the properties of each gas and turbine efficiency. Most of them were compatible to start up the LRE however air was properly used only for low power mode of turbine.