• The Korean Journal of Physiology and Pharmacology
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• v.13 no.6
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• pp.483-489
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• 2009

Despite the potential importance of the human regulator of calcineurin 1 (RCAN-1) gene in the modulation of cell survival under stress, little is known about its role in death-inducing signal pathways. In this study, we addressed the effects of RCAN1.4 knockdown on cellular susceptibility to apoptosis and the activation of death pathway proteins. Transfection of siRNAs against RCAN1.4 resulted in enhanced Fas- and etoposide-induced apoptosis, which was associated with increased expression and translocation of Bax to mitochondria. Our results suggest that enhanced expression and activation of p53 was responsible for the upregulation of Bax and the increased sensitivity to apoptosis, which could be reversed by p53 knockdown. To explain the observed upregulation of p53, we propose a downregulation of the ubiquitin ligase HDM2, probably translationally. These findings show the importance of appropriate RCAN1.4 expression in the modulation of cell survival and reveal a link between RCAN1.4 and p53.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.10a
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• pp.259-261
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• 2021

In this paper, VR smart factory XR content tailored to process execution scenarios was produced to reflect the characteristics of XR content, which is highly dependent on HDM, unlike general-purpose content software for UX/UI design and usability evaluation of digital twin-based XR content in manufacturing. HMD equipment called Qculus Quest2 was used to perform the process execution task scenario for XR content, and content production was made through Unity Engine and SteamVR Plugin.

• Allergy, Asthma & Immunology Research
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• v.10 no.6
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• pp.662-674
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• 2018

Purpose: Group 2 innate lymphoid cells (ILC2s) have been implicated in the pathogenesis of allergic disease. However, the effect of allergen-specific immunotherapy (AIT) on ILCs remains to be clarified. The aim of this study was to evaluate the levels of ILC subsets in allergic rhinitis (AR) patients in response to house dust mite (HDM)-specific immunotherapy. Methods: We enrolled 37 AR patients undergoing AIT (16 responders and 11 non-responders) for 2 years, 35 HDM AR patients and 28 healthy subjects. Peripheral blood mononuclear cells (PBMCs) were analyzed by flow cytometry to identify ILC subsets. Stimulation of ILC2s with recombinant allergen-specific protein was used to determine ILC2's activation (CD69 expression). Results: Responder AIT patients and healthy subjects had a decreased frequency of circulating ILC2s compared to non-responder AIT and AR patients. Conversely, ILC1s from responder AIT patients and healthy subjects showed increased frequency compared to non-responder AIT and AR patients. The frequency of ILC3s natural cytotoxicity receptor $(NCR)^+$ and $NCR^-$ in responder AIT patients was significantly lower compared to AR patients and healthy subjects. The ILC1: ILC2 proportion in responder AIT patients was similar to that of healthy subjects. PBMCs from patients who were responders to AIT had a significantly lower expression of the activation marker CD69 on ILC2s in response to allergen re-stimulation compared to AR patients, but no difference compared to non-responder AIT patients and healthy subjects. Conclusions: We propose that AIT might affect ILC responses. The activation of ILC2s was reduced in AR patients treated with AIT. Our results indicate that a relative ILC1/ILC2 skewed response is a possible key to successful AIT.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.68-68
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• 2016

하천의 사행 및 합류 형상은 복잡한 나선 흐름을 발생시킨다. 그 중 이차류는 사행에 의한 편수위 형성과 횡단 압력의 불균형, 그리고 수심에 따라서 변화하는 원심력에 의하여 형성된다. 이러한 흐름은 주로 3차원 모형으로 재현할 수 있으며 이에 관련된 연구는 계속 이루어졌으나 3차원 모형의 구성과 사용에는 상당한 시간 및 노력이 요구된다. 본 연구에서는 이러한 이차류의 영향을 수심적분된 2차원 모형으로 구현하고자 하였으며 이를 위해 이차류 연직 분포에 대한 기존 연구를 확인하고, 이러한 연직 분포를 표현할 수 있는 경험식을 3차원 모형인 FLOW3D를 통해 모의하고 개발하였다. 3차원 모형을 다양한 사행반경을 갖는 실험 사행수로에 적용하여 사행도에 따른 민감도를 분석하고 경험식을 개발하였다. 개발된 식은 2차원 수리해석 모형인 RAMS(River Analysis and Modeling System)에 적용되었다. RAMS는 수심 적분된 2차원 천수 방정식을 지배방정식으로 사용하는 수리해석모형인 HDM-2D를 해석엔진으로 사용하며, 이차류의 영향을 반영하기 위하여 개발한 수직분포 경험식을 분산 응력항 형태로 적용하였다. 모형의 적용성 확인을 위하여 사행 실험수로 및 합류수로에 모의를 수행하였다. 사행수로 및 합류수로는 실험 경계조건과 동일한 상류 입력 유량과 하류 경계조건을 사용하여 결과를 비교하였다. 사행수로 모의 결과 유속분포의 거동이 실험수로의 사행으로 인하여 2차류 효과로 주 흐름이 바깥쪽으로 기울어짐을 일부 재현하였다. 합류수로의 경우 기존 모형에 비하여 분산 응력항이 포함된 본 모형이 실측값에 근접하였는데 이는 이차류가 발생하는 영향으로 하류에 유속편차가 줄어드는 영향을 더 정확히 묘사한 것으로 판단된다.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.37 no.5
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• pp.879-890
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• 2017

The US Pavement Design method (AASHTO) and HDM-4, a road pavement maintenance decision system, are not suitable for domestic pavement design, construction and maintenance. KPRP(Korea Pavement Research Program) has been developed to reflect Korea's environmental conditions and vehicle characteristics, thereby, extending pavement life. The main objective of this study is to select the best alternative through Life Cycle Cost $CO_2$ (LCCC) calculations among three representative maintenance strategies using KPRP design software since the environment cost resulting from the extended pavement life will also differ. The analysis of this study illustrates that cumulative carbon emissions for 40 years in alternative 2 (Cutting and Overlaying at Year 30) is the lowest option among them, and the basic cost of $CO_2$ emission by various road maintenance and repair work can be used for suggesting an optimal maintenance strategy for highway agency.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.300-300
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• 2020

최근 전체 산업재해율은 계속적으로 감소 추세를 보이는 반면, 건설업의 재해율은 증가하는 추세를 내타내고 있다. 이러한 건설업 공종별 재해유형은 임시구조물과 관련된 가설공, 굴착공, 철근콘크리트공의 재해율이 타 공종에 비해 높게 나타난다. 이는 공종 진행에 따라 변화되는 건설현장의 위험요소를 사전에 인지하여 제어·관리할 수 있는 사고예측 기술 및 대응 기술의 부재로 안전사고가 높게 발생한 것으로 판단된다. 이러한 상황 속에서 건설현장에서 빈번하게 발생하는 다양한 재해 상황에 대하여 공사별, 규모별, 공정별 대응 체계를 마련하고, 굴착공사 중 침수 발생 시 피해를 최소화할 수 있는 실용적인 긴급대응 방안을 확보함으로써 경제적인 손실뿐만 아니라 인명피해를 최소화하는 방안 마련이 필요하다. 공사 현장 재해 상황 중 침수는 태풍 또는 집중호우 시 공사구간이 침수되어 근로자 수몰 등의 피해가 발생하고, 절토면이 붕괴되는 등 2차적인 피해로 이어진다. 이러한 침수의 원인은 공사 현장의 저지대 위치, 집중호우 시 최대 강우량에 대한 대비 소홀, 양수기 부족 및 고장, 인접 배수로 배수기능 불량 등의 복합적인 원인들로 인하여 발생한다. 또한 굴착공사 현장의 침수는 공사기간 동안 개별 공종에 따라 침수발생 양상이 달라질 수 있다. 따라서 각 공종별 발생 가능한 침수 양상에 대한 예방/대응 기술이 필요하다. 본 연구에서 사용되는 HDM-2D 수리 동역학 모델은 2차원 흐름해석 프로그램으로 복잡한 하천지형이나 공사현장과 같은 불연속면에서 유속 및 수심 등을 계산한다. 또한 마름/젖음 현상 등 복잡한 수리현상에 대한 정교한 모의 기능 및 침수해석 등 어떠한 계산영역이나 흐름조건에서도 정확하고 안정적인 모의를 할 수 있다. 본 연구에서는 공사현장과 같은 불연속면에서 각 공종에 따라 발생할 수 있는 침수양상에 대한 모의를 진행하고 각 공종별 침수양상을 비교분석 하였다. 이를 통해서 굴착공사 현장 침수피해 예측 모델을 개발하여 개발된 기술을 사용하여 기후변화에 따른 강우량 증가와 이에 따른 수재해로 인한 건설현장 침수 발생 및 인명, 재산피해를 최소화하고자 한다.

• Journal of Haehwa Medicine
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• v.15 no.2
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• pp.121-134
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• 2006

This study saw the anti-allergy effect by the immunity regulation action of Yanghyelyoonbotang (YHYBT) consists 12 kinds of herbal medicine agents. Consequently, YHYBT controlled the amount of secretion of various infla- mmatory cytokines, chemokine, monocyte chemotactic protein and histamine from cells (HMC-1, THP-1, EoL-1) stimulated by PMA, A23187 or HDM. 1. YHYBT did not show cytotoxicity on cultured human fibroblast cells under 250 ${\mu}g/m\ell$ concentration. 2. YHYBT suppressed IL-8, TNF-$\alpha$, IL-6 mRNA expression in the HMC-1 cell stimulated with PMA and A23187. 3. YHYBT significantly suppressed IL-6 release in the THP-1 and EoL-1 cell stimulated with HDM. 4. YHYBT significantly suppressed histamine release in the HMC-1 cell stimulated with PMA and A23187 in a dose-dependent. 5. YHYBT significantly suppressed $\beta$-Hexosaminidase release in the HMC-1 cell stimulated with A23187 in a dose-dependent. 6. YHYBT suppressed NF-$\kappa$B gene expression in the RBL-2H3 cell stimulated with PMA in a dose-dependent. These results suggested that YHYBT has suppressive effects on allergic reaction and pro-inflammatory cytokines in various cell lines through the regulation of immune system. YHYBT has potential to use as an antiallergic agents.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.43 no.2
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• pp.231-238
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• 2023

Since there is a limit to the physically visible horizon that sensors for autonomous driving can perceive, complementary utilization of digital map data such as a Local Dynamic Map (LDM) along the probable route of an Autonomous Vehicle (AV) is proposed for safe and efficient driving. Although the amount of digital map data may be insignificant compared to the amount of information collected from the sensors of an AV, efficient management of map data is inevitable for the efficient information processing of AVs. The objective of this study is to analyze the efficiency of information use and information processing time of AV according to the expansion of the active section of LDM-based static road and traffic information. To carry out this objective, a microscopic simulator model, VISSIM and VISSIM COM, was employed, and an area of about 9 km × 13 km was selected in the Busan Metropolitan Area, which includes heterogeneous traffic flows (i.e., uninterrupted and interrupted flows) as well as various road geometries. In addition, the LDM information used in AVs refers to the real high-definition map (HDM) built on the basis of ISO 22726-1. As a result of the analysis, as the electronic horizon area increases, while short links are intensively recognized on interrupted urban roads and the sum of link lengths increases as well, the number of recognized links is relatively small on uninterrupted traffic road but the sum of link lengths is large due to a small number of long links. Therefore, this study showed that an efficient range of electronic horizon for HDM data collection, processing, and management are set as 600 m on interrupted urban roads considering the 12 links corresponding to three downstream intersections and 700 m on uninterrupted traffic road associated with the 10 km sum of link lengths, respectively.

• BMB Reports
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• v.42 no.5
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• pp.299-303
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• 2009

In this study, we use promoter analysis to show that interaction between Jab1 and p53 induces suppression of p53 activation in U2OS and H1299 cells. Interaction between p53 and Jab1 was further confirmed by immunoprecipitation and immunofluorescent analyses. In particular, Jab1 was able to induce nuclear export of p53 as previously reported. When Jab1 was overexpressed in U2OS cells followed by etoposide or hydrogen peroxide ($H_2O_2$), cell death induced by such stresses was protected against. On the contrary, when the level of Jab1 was suppressed in U2OS cells, cytotoxicity imposed by etoposide and $H_2O_2$ was dramatically increased, suggesting a cell protective role for Jab1. These results indicate that Jab1 is a negative regulator of p53 and a plausible oncogene.

• Journal of Korea Water Resources Association
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• v.54 no.7
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• pp.495-507
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• 2021

With the recent industrial development, accidental pollution in riverine environments has frequently occurred. It is thus necessary to simulate pollutant transport and dispersion using water quality models for predicting pollutant residence times. In this study, we conducted a field experiment in a meandering reach of the Sum River, South Korea, to validate the field applicability and prediction accuracy of RAMS+ (River Analysis and Modeling System+), which is a two-dimensional (2D) stream flow/water quality analysis program. As a result of the simulation, the flow analysis model HDM-2Di and the water quality analysis model CTM-2D-TX accurately simulated the 2D flow characteristics, and transport and mixing behaviors of the pollutant tracer, respectively. In particular, CTM-2D-TX adequately reproduced the elongation of the pollutant cloud, caused by the storage effect associated with local low-velocity zones. Furthermore, the transport model effectively simulated the secondary flow-driven lateral mixing at the meander bend via 2D dispersion coefficients. We calculated the residence time for the critical concentration, and it was elucidated that the calculated residence times are spatially heterogeneous, even in the channel-width direction. The findings of this study suggest that the 2D water quality model could be the accidental pollution analysis tool more efficient and accurate than one-dimensional models, which cannot produce the 2D information such as the 2D residence time distribution.