• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.369-374
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• 2018

The purpose of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shoot and rooting for the in vitro mass production of Haworthia maughanii. To determine suitable parts of the plant for callus induction, the leaves, flower bloom and flower stalks were cultured in MS medium at different concentrations of $0{\sim}2mgL^{-1}$ NAA and $0{\sim}2mgL^{-1}$ TDZ, respectively. All of the parts showed 100% callus formation rate at $NAA\;1mgL^{-1}$ and $TDZ\;1mgL^{-1}$ treatment, $NAA\;2mgL^{-1}$ and $TDZ\;2mgL^{-1}$ treatment and NAA 1 to $2mgL^{-1}$, respectively. While the rate of callus formation was high in all parts, the leaves were the most efficient to obtain most culture parts. $NAA\;0.1mg\;L^{-1}$ and $BA\;0.1mg\;L^{-1}$ treatments were the most effective in shoot formation with 22.0 shoots. In addition, multiple shoot propagation showed 16.3 shoots, the highest, with $NAA\;0.1mg\;L^{-1}$ and $BA\;0.1mg\;L^{-1}$ treatments. These results led us to speculate that the optimization of culture conditions was responsible for the mass propagation for in vitro cultures of Haworthia maughanii.

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.127-135
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• 2019

The purpose of this study was to investigate suitable parts for callus induction and optimal concentrations of growth regulators, contained in the medium affecting shoot and rooting for in vitro mass production of Haworthia truncata. Leaves and flower bud showed 100% callus formation rate at NAA $1{\sim}2mgL^{-1}$ treatment, and NAA $1mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment. The flower stalk showed 75% callus formation rate, at NAA $2mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment in H. truncata. While the rate of callus formation was high in leaves and flower bud, leaves were the most efficient in obtaining most culture parts. Shoot induction rate from callus was highest, at NAA $0.1mgL^{-1}$ treatment in H. truncata. Additionally, the number of shoots formation was 66.3 shoots high, in NAA $1mgL^{-1}$ + BA $0.1mgL^{-1}$ treatment in H. truncata. In the case of acclimatization of regenerated plant, growth characteristics did not show significant difference (95%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate, considering plant height and appearance preference of H. truncata. It was established that optimization of culture condition, was responsible for mass propagation in vitro cultures of H. truncata.