• Title/Summary/Keyword: Harmonia axyridis

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Selection of the Acaricides Selective to Harmonia axyridis and Effect of their Application on Phytophagous Mites and Natural Enemies (무당벌레에 선택적인 살비제 선발과 이들 처리가 사과원 식식성 응애류와 천적류에 미치는 영향)

  • Cho, Jum-Rae;Hong, Ki-Jeong;Lee, Gwen-Seok;Park, Byung-Ryul
    • Korean journal of applied entomology
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    • v.35 no.3
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    • pp.243-248
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    • 1996
  • This study was conducted to select the acaricides selective to H. axyridis and examine the effects on phytophagous mites and natural enemies after application of selected acaricides in the apple orchard. All the acaricides tested were more toxic to T. urticae and P. ulmi than to H. axyridis, and also were more toxic to egg than to the larva and adult of H. myridis. Azocyclotin and fenpropathrin were the highest and lowest selective acaricides to H. axyridk, respectively. The density of phytophagous mites was high affected by frequency of acaricide application. Significant difference in the changes of the density of natural enemies was not be observed according to frequency of acaricide application and kinds of acaricide. Acaricide treatment showed high effect on the population densities of A. womersleyi and Oriur spp., while less effect on Chrysopa spp.

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Isolation and Characterization of Acid Protease Produced by Staphylococcus sp. CB2-3 from Digestive Organ of Harmonia axyridis (무당벌레 소화기관으로부터 산성 단백질 분해효소를 생산하는 Staphylococcus sp. CB2-3의 분리 및 특성)

  • Kim, Se-Jong;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.47 no.3
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    • pp.255-262
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    • 2011
  • Six protein-degrading bacteria were isolated from digestive organ of Harmonia axyridis. These isolates were categorized as Staphylococcus sciuri subsp. sciuri (3 strains), Bacillus subtilis (1 strain), and Bacillus thuringiensis (2 strains) by 16S rRNA gene sequence analysis. The Staphylococcus sp. CB2-3 was selected as a protease-producing bacterium which showed the highest protease activity of 58.5 U/ml at the pH 5.0 medium. The optimal pH and temperature of protease activity were pH 5.0 and $40^{\circ}C$, respectively. This acid protease had a relatively high stability of 80% between $30-50^{\circ}C$ at broad temperature range. The opimal medium compositions of carbon, nitrogen and mineral source for cell growth and protease activity were investigated. When sorbitol (0.5%) was used as carbon source, enzyme activity was increased about 2 times than that of the basal medium. When skim milk (0.5%) was used as nitrogen source, activity was increased about 2.5 times than that of the control. Cell growth and enzyme activity were increased by mineral source such as KCl, $K_2HPO_4$, $FeSO_4$, but was completely inhibited by divalent ions such as $Co^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Cu^{2+}$.

Effects of Gamma Irradiation on the Survival and Development of the Multicolored Asian Ladybird Beetle, Harmonia axyridis (Coccinellidae; Coleoptera) (감마선이 무당벌레의 생존과 발육에 미치는 영향)

  • Seo, Mi-Ja;Lee, Hee-Jin;Kang, Eun-Jin;Kang, Si-Yong;Yu, Yong-Man;Youn, Young-Nam
    • Korean journal of applied entomology
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    • v.50 no.4
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    • pp.335-342
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    • 2011
  • The effects of gamma irradiation on the survival, development, fecundity, and sex ratio of the multicolored Asian ladybird beetle (Harmonia axyridis) were investigated. A dose of more than 50 Gy dose of irradiation on eggs completely inhibited hatch, and 1st and 3rd instar larva irradiated with over 40 Gy almost died. Adverse effects of gamma irradiation over 70 Gy were also observed in the survival and development of pupae and adults. Although irradiated individuals usually survived, they had longer periods of larval and pupal development than untreated individuals. Slight female-biased sex ratios were observed in some treatments. However, sex ratio differences soon equilibrated. Generally, inherited harmful effects of gamma irradiation were observed in F1 and F2 generations. Comet tail indicating DNA damage was showed in survival individuals exposed to gamma irradiation.

Effects of Environment Friendly Agricultural Materials to Each Developmental Stages of Harmonia axyridis(Coleoptera: Coccinellidae) in the Laboratory (실내조건에서 무당벌레(Harmonia axyridis : Coleoptera: Coccinellidae)의 각 발육단계에 친환경농자재가 미치는 영향)

  • Kang, Eun-Jin;Kang, Myong-Ki;Lee, Hee-Jin;Lee, Dae-Hong;Seok, Hee-Bong;Kim, Da-A;Gil, Mi-La;Seo, Mi-Ja;Yu, Yong-Man;Youn, Young-Nam
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.97-107
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    • 2007
  • The multicolored Asian ladybird beetle (Harmonia axyridis) has been commonly used with biological control agents for control of several kinds of aphids in agroecosystems. Also, environment friendly agricultural materials have been commonly applied in crop fields because the government held down pesticide application and environment friendly agricultures are gradually increased with consumer's desires. The multicolored Asian ladybird beetles may be directly or indirectly under the influence of environment friendly agricultural materials In crop fields. The insecticidal environment friendly agricultural materials (IEFAMs) might be saff against each developmental stage of multicolored Asian ladybird beetle. Fungicidal environment friendly agricultural materials (FEFAMs) had a miner effect to each developmental stage of multicolored Asian ladybird beetle with the exception of FEFAM A. Environment friendly agricultural materials contained useful soil microorganisms (EFAMSMs) C and H might be down the hatching rate of eggs, and EFAMSM A and F had a killing effect to 1st instar of lady beetles. Environment friendly agricultural materials contained plant extracts (EFAMPEs) A and D might be suffered effect a deathblow of egg hatching with lady beetles. Otherwise, there was a miner effect to lady beetles with the rest of tested environment friendly agricultural materials.