• Asian-Australasian Journal of Animal Sciences
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• v.22 no.11
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• pp.1487-1494
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• 2009

The major histocompatibility complex (MHC) plays well-defined roles in eliciting immune responses and combating infectious diseases. The major histocompatibility complex of cattle is referred to as BoLA (Bovine Lymphocyte Antigen). This genetic system is among the most polymorphic. In the present study, polymorphism of the BoLA- DRB3.2 locus in three Bos indicus breeds viz., Sahiwal, Rathi and Hariana was studied by polymerase chain reaction restriction fragment length polymorphism technique using the enzymes RsaI, Bst Y1 and Hae III. Both Sahiwal and Rathi are good Indian dairy breeds and survive under tough tropical conditions, while Hariana is a prominent dual-purpose breed reared both as a dairy animal and for bullock production. A total of 30 different BoLADRB3.2 alleles were observed to be present in the 3 Bos indicus breeds. Certain alleles were common amongst the three breeds while there were others that were unique to each breed. Allelic distribution amongst the three breeds showed that each breed had a unique allelic distribution pattern that was different from each other and also different from the earlier breeds studied so far for the existence of allelic variation at this locus. A dendogram was constructed based on the frequencies of the BoLA-DRB3 alleles using the UPGMA method. The Rathi and Hariana animals were genetically the most apart. The Hariana animals clustered on a different branch from the other two breeds viz. the Rathi and the Sahiwal. The smallest genetic distances for the DRB3 alleles were those between Sahiwal and Rathi (0.5461) while genetic distance between Hariana and Sahiwal was 0.6123. A comparison of the allelic frequencies of the BoLADRB3.2 locus in these 3 breeds viz. Sahiwal, Hariana and Rathi with the allelic frequencies present in the previously characterized Bos indicus Kankrej breed, which is a dual purpose breed reared both as a draught and a dairy animal, showed that the Bos indicus Sahiwal and Rathi breeds clustered into one group while the Hariana and Kankrej breeds formed another group. The Rathi and Sahiwal showed the least genetic distance of 0.5461 amongst the breeds whereas the Rathi and Kankrej, with a Nei''s genetic distance of 1.1622, were genetically the most distant apart.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.8
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• pp.1062-1066
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• 2001

Studies were carried out on forty young bulls of the Hariana breed (around 18 months of age) selected on the basis of their dam's milk yield at the animal farm of CCS HAU, Hisar during the period 1993-1999. Since animals showed variation in capacity to work over prolonged periods, they were subjected to regimes of carting without load and with 8 qtls. of load for three hours. The study revealed that maximum change took place in the pulse rate, followed by respiration rate and rectal temperature. The mean fatigue score for pulling an empty cart ranged between 1.66 to 2.20 after two hours work, and 2.36 to 2.73 after three hours work. For a cart loaded with 8 qtls., the corresponding ranges were 2.90 to 3.36 and 3.40 to 4.10, respectively. These results indicated that the animals under experiment had the capacity to pull moderate load (8 qtls.) for about two hours without showing any serious effect on the ability to recover and soon to work again.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.8
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• pp.1067-1072
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• 2001

An experiment was conducted on eight bullocks of Hariana cattle study draft efficiency using different appliances, changes in their physiological, biochemical and haematological parameters, and how the body tries to maintain these within normal physiological range. Blood collection was done at pre-exercise, three hours after exercise and two hours post exercise in summer and winter seasons of the year 1990-91. Average pulse rate and respiration rate per minute were found to be $63.09{\pm}0.78,\;25.60{\pm}0.54,\;97.21{\pm}2.51,\;63.37{\pm}2.70,\;64.05{\pm}1.90,\;25.33{\pm}1.20$, respectively, at pre-exercise, three hours exercise and two hours post exercise rest. The seasonal differences were found to significant and the values were higher in summer than in winter. After two hours post exercise rest Hariana bullocks regained their normal values for all the parameters, except that pulse rate, respiration rate and serum sodium during summer did not reach the pre - exercise levels.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.466-469
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• 2002

The data on 512 Hariana cows, progeny of 20 sires calved during period from 1974 to 1993 maintained at Government Livestock Farm, Hisar were considered for the estimation of genetic parameters. The means for first lactation milk yield (FLY), wet average (WA), first lactation peak yield (FPY), first lactation milk yield per day of first calving interval (MCI) and first lactation milk yield per day of age at second calving (MSC) were 1,141.58 kg, 4.19 kg/day, 6.24 kg/day, 2.38 kg/day and 0.601 kg/day, respectively. The effect of period of calving was significant (p<0.05) on WA, FPY and MCI while the effect of season of calving was significant only on WA. Monsoon calvers excelled in performance for all the production efficiency traits. The effect of age at first calving (linear) was significant on all the traits except on MCI. Estimates of heritabilty for all the traits were moderate and ranged from 0.255 to 0.333 except for WA (0.161). All the genetic and phenotypic correlations among different production efficiency traits were high and positive. It may be inferred that selection on the basis of peak yield will be more effective as the trait is expressed early in life and had reasonably moderate estimate of heritability.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.5
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• pp.470-474
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• 1998

Data on the first lactation performance traits of $F_1$, $F_2$ and $F_3$ crosses covering the period from 1972 to 1995 of a total of 803 dairy cows of three genetic grades maintained at Livestock Production Research Farm, Indian Veterinary Research Institute, Izatnagar were analysed. Three genetic grades were 1/2 Friesian + 1/2 Hariana (FH), 1/2 Friesian + 1/4 Brown Swiss and 1/4 Hariana (FBH) and 1/2 Friesian+ 1/4 Jersey + 1/4 Hariana (FJH). Age at first calving increased by 7% and 8% in $F_2$ and $F_3$, respectively, over the $F_1$ in FH. The reduction in age at first calving at $F_2$ and $F_3$ levels by 2-7% over the $F_1$ was observed in FBH and FJH. The lactation milk yield of $F_1$, $F_2$ and $F_3$ crosses was $1,943{\pm}100.3$, $2202{\pm}120.5$ and $1,925{\pm}123.2kg$ in FH; $2,014{\pm}76.7$, $2,264{\pm}91.5$ and $2,096{\pm}123.9kg$ in FBH and $2,005{\pm}87.0$, $2,414{\pm}94.4$ and $2,093{\pm}121.1kg$ in FJH, respectively. The lactation milk yield improved by 12-20% in $F_2$ crosses in various genetic grades. The performance of $F_1$ was, however, maintained in FH $F_3$ crosses, it improved by 4% in FBH and FJH $F_3$ crosses. The lactation lengths and calving intervals were nearly the same for $F_1$, $F_2$ and $F_3$ crosses in FH while lactation lengths and calving intervals were reduced by 3-11% in $F_2$ and $F_3$ crosses in FBH and FJH genetic grades. The milk yield/day of lactation length and milk yield/day of calving interval increased by 16-35% in $F_2$ and 2-14% in $F_3$ over the $F_1$ in various genetic grades. It is recommended that a sufficiently large effective population size of these three genetic grades be maintained by inter se matings and rigorous selection of sires so for developing a genetic base population for new breed development.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.7
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• pp.898-904
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• 2011

The PCR- restriction fragment length polymorphism (RFLP) in and around TM4 of SLC11A1 gene and its association with the incidences of brucellosis in Hariana breed (Bos indicus) and Holstein Friesian crossbred (Bos indicus${\times}$Bos taurus) cattle was examined. A fragment of 954 bp encoding the TM4 was amplified, and RFLP was identified by digestion of the amplicon independently with AluI and TaqI. The amplicon (GenBank Acc. No. AY338470 and AY338471) comprised of a part of exon V (<59 bp) and VII (62>), and entire intron 5 (423 bp), exon VI (71 bp) and intron 6 (339 bp). Digestion with AluI revealed the presence of two alleles viz, A (281, 255, 79 and 51 bp) and B (541, 255, 79 and 51 bp). The frequency of A allele was estimated as 0.80 and 0.73 in Hariana and crossbred cattle, respectively. Due to presence of a polymorphic TaqI site at intron 5, two alleles: T (552 and 402 bp) and Q (231, 321 and 402 bp) were identified. The frequency of T allele was estimated as 0.96 and 0.97, respectively. For association study, on the basis of serological tests and history of abortion, the animals were grouped into "affected" and "non-affected". However, no association could be established with the observed RFLPs.