• Korean Journal of Animal Reproduction
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• v.17 no.3
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• pp.183-191
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• 1993

These experiments were carried out to investigate whether the enzyme is involved in zona hardening during normal activatin of the oocytes by sperm, and demonstrate peroxidase activity during in vitro fertilization of oocytes treated with peroxidase inhibitors(250 $\mu$M phenylhydrazine, 28mM sodium sulfite, 350mM glycine ethyl ester and 50mM sodium azide) and tyrosine analogue(12.5mM tyramine). Also, zona soluble properties of the ovarian oocytes incubated for 0, 5, 10 and 15 hr in the presence of pheylhydrazine or tyramine were studied by using $\alpha$-chymotrypsin. The results obtained from these experiments were summarized as follows ; 1. The rates of fertilizatin in control oocytes and oocytes treated with phenylhydrazine or tyramine were 69.8%, 62.3% and 88.2%, respectively. However in vitro fertilization in oocytes treated with three different peroxidase inhibitors, sodium sulfite, glycine ethyl ester and sodium azide, were not induced. The oocytes treated with phenylhydrazine had no significant effect on in vitro fertilization rate as compared to control. However there was a significantly different in fertilization between tyramine treated group and control group(P<0.01). 2. The zona solubility(t50) of control and fertilized oocytes in culture treated with phenylhydrazine or tyramine were 30.7, 26.0 and 16.3 min., respectively. Phenylhydrazine treated group and tyramine treated group had effect on inhibition of zona hardening as compared to control group. These results suggest that ovoperoxidase is involved in zona hardening during normal activation of the oocytes by sperm. 3. t50 of control oocytes and ovarian oocytes treated with phenylhydrazine or tyramine for 5, 10 and 15 hr in vitro were 14.0, 26.2 and 32.0 min., 14.5, 26.9 and 30.2 min., and 14.0, 24.3 and 31.2 min., respectively. These results suggest that zona hardening in ovarian oocytes matured for various times in vitro cannot be inhibited by peroxidase inhibitors and tyrosine analogue, that the spontaneous zona hardening incultured ovarian oocytes is not caused by the secretory products of cortical granules released during the cortical reaction, ovoperoxidase.

• Korean Journal of Animal Reproduction
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• v.17 no.3
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• pp.173-181
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• 1993

In order to demonstrate whether ovoperoxidase hardens the zona of oocytes activated by incubating in M-S buffer supplemented with 20$\mu$M of Ca-ionophore A 23187, the effect of peroxidase inhibitors(250mM pheylhydrazine, 28mM sodilum sulfite, 350mM glycine ethyl ester and 50mM sodium azide), tyrosine analogue(12.5mM tyramine) and exogeneous peroxidase(50$\mu\textrm{g}$/ml horseradishperoxidase ; HRP) on zona hardening in ionorphore-treated oocytes were investigated. The results obtained from thses experiments were summarized as follows : 1. The zona solubility (t50) of ionophore-activated and DMSO-treated oocytes at 1, 2 and 3 hr of culture were 25.0, 31.6 and 40.6min., and 9.7, 10.8 and 15.5 min., respectively. The longest time required for zona lysis of ionophore activated oocytes at 1 hr after onset of ionophore treatment. The diferences int50 for zona was significantly greater as compared to DMSO-treated controls(P<0.01). 2. The inhibition rates of hardening in the oocytes treated with the phenylhydrazine, sodium sulfite, glycine ethyl ester and sodium azide, were 23.8, 61.9, 95.2 and 23.8%l, respectively, and the tyramine, was 14.3%. Several known peroxidase inhibitors and tyrosine analogue were blocked zona hardening in ionophore activated oocytes. 3. The treatment of exogeneous peroxidase promoted the zona hardening of activated oocytes but not unactivated oocytes. These resuls indicate that the ovoperoxidase apparently catalyzes the hardening of the zona following ionophore activation of mouse oocytes.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.2C
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• pp.75-81
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• 2008

In this study, a series of laboratory column test on Suction Drain Method which is one of the way to make an soft ground improvement were conducted in order to investigate the effect of the Hardening Zones and the ratio of improvements depending on periods of the improvements and various applied suction pressures. On this occasion, the experimental conditions are followings; in the case of the periods of effectiveness, 4 days, 8 days, 12 days, 16 days, 20 days and in the case of the applied pressures of the Suction are -20 kPa, -40 kPa, -60 kPa and -80 kPa were carried out. As a result of test, settlement increased with suction pressure and duration increase, and gradually converged. Also, as comparing permeability decrease ratio with which calculated back from water content and numerically predicted using Hansbo's radial consolidation theory, measured value was almost coincide with predicted value when permeability decrease ratio was assumed as 2~3. Furthermore, the hardening zone was appeared within 7~8 cm of whole radial (25 cm).

• Proceedings of the KWS Conference
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• 1992.10a
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• pp.156-161
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• 1992

• Journal of Korean Society of Steel Construction
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• v.29 no.1
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• pp.1-12
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• 2017

This paper proposes nominal flexural strength considering strain-hardening effect of HSB600 high performance steel for compact composite I-girders in positive bending. Unlike conventional steels, HSB600 undergoes strain-hardening just after yielding without going through yield plateau. However, because the nominal flexural strength specified in domestic and foreign bridge design specifications has been developed for the conventional steel composite girders, the nominal flexural strength does not appropriately consider the strain-hardening of HSB600. Therefore, plastic moment considering a strain-hardening is proposed so as to consider effect of the strain-hardening of HSB600 on flexural strength and then moment-curvature analysis is performed to a wide range of cross-sections. From results of the analysis, a parameter representing the effect of the strain-hardening on the flexural strength of HSB600 composite girders is proposed. Furthermore, by using this parameter, the nominal flexural strength considering the strain-hardening effect for HSB600 composite I-girders in positive bending is proposed and then evaluated by comparing with the current AASHTO LRFD bridge design specifications.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.3
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• pp.529-539
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• 1994

In laser heat treatment process of steels, the thin layer of substrate is rapidly heated to the austenitizing temperature and subsequently cooled at a very fast rate due to the self-quenching effect. Consequently, it is transformed to martensitic structure which has low magnetic permeability. This observation facilitates the use of a sensor measuring the change of electromagnetic field induced by the hardening layer. In this paper, the eddy-current electromagnetic field is analyzed by a finite element method. The purpose of this analysis is to investigate how the electrical impedance of the sensor's sensing coil varies with the change in permeability. To achieve this, a numerical model is formulated, taking into consideration the hardening depth, distance of the sensor from the hardened surface and the frequency driving the sensor. The results obtained by numerical simulation show that the eddy-current measurement method can feasibly be used to measure the changing hardening depth within the frequency range from 10 kHz to 50 kHz.

• Journal of Ocean Engineering and Technology
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• v.23 no.2
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• pp.74-80
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• 2009

Surface hardening treatments, such as using the high-frequency induction hardening method, are widely used to increase the fatigue life and prevent the failure of materials by locally increasing the surface hardness. This method, in particular, brings an improvement in static strength by compressive residual surface stress due to the hardening. In this study, the mechanical properties of high-frequency induction hardened SCM440 steel were investigated. These results were also compared with those for base metal and a Q/T (tempering after quenching) treatment specimen. The test results showed that partially high-frequency induction hardened SCM440 steel specimens were more improved in static strength, surface hardness, fatigue limit, and anti-wear than the base metal and Q/T treatment specimens. In particular, the fatigue limit of the high-frequency induction hardened SCM440 steel increased by more than about 52% compared to that of base metal and by about 25% compared to that of the Q/T specimen.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2012.11a
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• pp.255-257
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• 2012

Epoxy resin without any hardener can harden in the presence of hydroxide ions in cement mortars and concretes at ambient temperature. The purpose of present study is to examine the hardening properties of hardener-free epoxy-modified mortars by curing conditions. The hardener-free epoxy-modified mortars using diglycidyl ether of A epoxy resin are prepared with various polymer-cement ratios, and subjected to initial moist/dry curing, initial steam(90℃) curing, initial steam/heat(80℃, 100℃) curing.As a result, degree of hardening of epoxy resin in initial moist/dry cured, initial steam cured and initial steam/heat(80℃) cured hardener-free epoxy-modified mortars is decreased with increasing polymer-cement ratio. However, it is markedly improved with additional dry-curing periods. On the other hand, regardless of the polymer-cement ratio and dry curing periods, degree of hardening of hardener-free epoxy-modified mortars with initial steam/heat(100℃) cure is over 95%.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.2
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• pp.108-111
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• 2000

Osmoconditioning and hardening effects for the seed germination of normal and naturally aged rice seeds were studied through analyzing the total sugars and $\alpha$-amylase activity. The normal seeds which used to have high germination rate accelerated germination with the osmoconditioning at the suboptimal temperature of 17$^{\circ}C$. On the other hand, the aged seeds did not affect germination rate at $25^{\circ}C$, while increased germination rate and accelerated germination a little at 17$^{\circ}C$, Hardening of aged seeds increased germination rate by 10-15% compared with control seeds at both 17 and $25^{\circ}C$ and accelerated germination. Total sugar content and $\alpha$-amylase activity of normal seeds were higher than did aged seeds. The aged seeds with treatment of osmoconditioning and hardening increased total sugar content and $\alpha$--amylase activity, but hardening was more effective than osmoconditioning. The $\alpha$--amylase activity was positively correlated with the total sugar content and germination rate.

• The KIPS Transactions:PartA
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• v.11A no.4
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• pp.227-234
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• 2004

A panic state is often caused by careless computer control. It could be also caused by a kernel programmer's mistake. When panic is occurred, the process of the panic state has to be checked, then if it can be restored, operating system restores it, but if not, operating system runs the panic function to stop the system in the kernel hardening O.S. To decide recovery of the process, the type of the panic for the present process should be checked. The value type and the address type have to restore the process. If the system process has a panic state, the system should be designed to shutdown hardening function in the Linux operating system.