• Clinical and Experimental Reproductive Medicine
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• v.24 no.1
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• pp.1-11
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• 1997

It is well known that the zona pellucidae of mouse oocytes become "hardened" when they are allowed to mature in vitro in the absence of serum components. To see if oocytes already undergone meiotic resumption in vivo exhibit similar zona hardening, hardening of ZP of cumulus-enclosed oocytes(CEOs) was examined after culture in vitro since their release from follicles various hours after hCG injection. When CEOs matured in vivo for 3h or longer were subjected to culture in vitro for 14h with BSA alone, zona hardening was significantly reduced compared to those cultured in vitro from the begining of maturation. However, when CEOs matured in vivo for 5h were freed from cumulus cells and then cultured in vitro with BSA alone, little reduction of zona hardening was observed. Preincubation of CEOs for 5h with fetuin, one of the well known inhibitor of in vitro zone hardening, did not prevent zona hardening during its subsequent culture of CEOs for 14h without fetuin. However, when CEOs precultured with both fetuin and PMSG for 5h and then further cultured with BSA alone for 14h, zona hardening was dramatically reduced. Under these conditions, the expansion of cumulus cell was observed. In addition, CEOs cultured with both BSA and dbcAMP to prevent their meiotic resumption showed a significant increase of zona hardening. Whether the observed zona hardening was correlated with the conversion of ZP2 to $ZP2_{f}$ was examined. Zona pellucida, isolated from CEOs matured for 5h in vivo and then further cultured with BSA alone was subjected to SDS-PAGE. Most of ZP2 molecules from these CEOs did not undergo conversion from ZP2 to $ZP2_{f}$. From these results, it is concluded that CEOs undergone meiotic resumption in vivo do not exhibit zona hardening when they were subsequently cultured in vitro without serum components. It appears that cumulus cells play an important role in this phenomenon.

• Journal of Korean Society of Forest Science
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• v.58 no.1
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• pp.34-40
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• 1982

Main consideration of this trial is to know whether the planting work should be possible to do not only in the early spring but also in the summer or autumn, for giving the guide to get the work plan and to broaden the employing season of the skilled forest worker. Seedling of Pinus koraiensis, Larix leptolepsis, Pinus rigida, Pinus rigida${\times}$ P. taeda(wind) and Chamaecyparia obtusa as the test species had been planted in 15 days interval from the middle of March to the end of November. The seedling survival was investigated in the spring time of coming year because the winter damage could be problems. At the same time the climate data was measured daily and the shoot growth of test species were also measured in other near plantation at 15 days interval to know the influence to survival. From these results the spring and autumn planting is showing the good survival and the summer planting seems to give the difficulties. The spring planting in the southern temperate zone could be stared earlier as the end of February or beginning of March because the soil temperature are increasing up more $5^{\circ}C$ from this time. But the summer planting from the beginning of May until the end of August in better to avoid with excluding specially the good season of rainfall distribution because of the shoot growth of green confer seedling and the leave sprouting of Larix leptolepsis are so vigorously growing up from the begining of May and its wood structure is too weak to compensate the water loss. But among the test species Pinus koraiensis and Chamaecyparis obtusa have more possibility to plant in the summer season. The autumn planting seems to be very reasonable to accept newly in the trial region. This may be the reasons of still high soil temperature to grow the seedling root and of hardened school to resist from the dry winter wind. But it will be carefully that the strongly exposured site could be to avoid for the autumn planting in case of specially Pinus rigida${\times}$P. taeda and Chamaecyparis obtusa. From these discussion the guide table 1 for planting season with the test species is proposed and can be used for planing and employing in the trial zone.