• 한국수정란이식학회지
• /
• 제15권1호
• /
• pp.47-56
• /
• 2000

This study was carried out to improve a technique of embryo transfer for twin calves production in Hanwoo cattle. Blastocysts for the donor of embryo transfer were classified into three criteria by accessment of morphology; early blastocyst, blastocyst and expanded blastocyst. Tow embryos were introduced transcervically into utrerine horn either of Hanwoo or Holstein by ipsilaterally or contralaterally to the corpus luteum. Thiry-six out of 57 recipients cows were inseminated by artificially on the next day of estrus, and followed by transfer of embryos into contralaterally. The pregnance rates of recipients following transfer of bovine embryos of day 7, 8 and 9 was 43.5, 18.2 and 8.3%, respectively. These results appeared that these was a significant (P<0.05) difference between on day-7 embryos and day-9 embryos, but not between on day-8 and day-9 embryos. Although there was not significant(P<0.05) difference in the pregnancy rates between the blastocysts(11/25, 44%) and expanded blastocysts(2/19, 10.5%) and between the blastocysts and early blastocysts(2/13, 15.4%), the embryos at blastocyst stage are more suitable than others for obtaining higher rate of pregnancy. There was no significant difference on pregnancy of the embryos transferred prior to presence(6/21, 29%) or absence (9/36, 25%) of artificial insemination. On pregnancy of Holstein, 2(15.4%) out of 13 recipients were pregnant in heifer. Similar Pregnancy rates were obtained between 1∼2 parities and 3∼4 parities by 30% (6/20) and 27.3%(3/11), respectively. Taken together, there was not significant difference in pregnancy rate due to small number of recipients used for this experiment. Both of Hanwoo and Holstein introduced the embryos by contralsterally to the corpus luteum were slightly higher pregnancy rate compare to by ipsilaterally (12/41, 29.3% vs, 3/16, 18.8%). The ratio of production of twin and single calves in Holstein was 20% (9/45) and 2.2% (1/45), respectively. However, in Hanwoo cows both of production of twin and single were similar as 8%. This result suggests that Holstein as recipients was superior to Hanwoo cows for production of twin calves. Out of all 15 pregnant, 12(80%) were produced a total of 22 normal calves in which the others composed of abnormal, as judging as 2(13.3%) for abortion and 1(6.6%) for stillbirth during the pregnant period.

• 대한생식의학회:학술대회논문집
• /
• 대한불임학회 1999년도 제38차 추계 학술대회
• /
• pp.47.1-47.1
• /
• 1999

• 한국동물번식학회:학술대회논문집
• /
• 한국동물번식학회 2001년도 춘계학술발표대회
• /
• pp.73-73
• /
• 2001

This study was to examine whether the vitrified, one-step diluted and direct transferred Hanwoo IVM/IVF/IVC blastocysts can be successfully survived in vivo and they were succeeded into the live birth. For vitrification, blastocysts were serially exposed in glycerol (G) or/and ethylene glycol (EG) mixtures [10% (v/v) G for 5 min, 10% G plus 20% EG (v/v) for 5 min, and 25% G plus 25% EG (v/v) for 30 sect] which is diluted in 10% FBS added D-PBS. Thawing of straw was carried out in air for 10 sec and then in water bath of $25^{\circ}C$ for 20 sec. One-step dilution within the straw was done in water bath of $25^{\circ}C$ for 1 min. Vitrified and one-step diluted embryos were directly transferred into 36 (natural or hormone induced synchronized) recipient cows in 6 areas of Kyungsang Buk-Do. Pregnancies were confirmed at first when recipient cows did not return to the subsequent estrus cycle, and later by manual palpation per rectum on day 45, 90 and then living calves were derived into parturition. Overall pregnancy was 33.3%(12/36), However, higher pregnancy was obtained when the recipients exhibited estrus one day earlier than the age of transferred embryos (53.3 vs 25.0-27.3%), irrespective of synchronization methods. Also, parous recipients became pregnant higher than nulliparous heifers, And, there were not different in pregnancy rates by the aspect of corpus luteum (CL) quality of recipients (good, 29.4; fair, 37.5; poor, 33.3%). One hundred eight of frozen-thawed Hanwoo blastocysts were directly transferred into 36 recipient cows. In 12 of pregnant cows, 3 cows were aborted and 9 cows were calved [single, 66.7% (6/9): twin, 33.3% (3/9)]. Total embryo implantation rate was 11.1% (12/108). However, 9 Hanwoo calves were lived. Therefore, these results demonstrate that direct transfer technique of vitrified and one-step diluted bovine blastocysts can be applied easily and effectively with the higher pregnancy rate on field trial without the equipment and embryological skills.

• 한국동물위생학회지
• /
• 제43권3호
• /
• pp.161-165
• /
• 2020

In order to effective treats a sick calf, it is necessary to obtain accurate information about the patient's condition. However, the standard references for Korean cattle claves are not well known. Therefore, this study aims to present useful clinical values by analyzing normal blood and diarrhea blood of Hanwoo calf. Recently, with the advent of a portable blood analyzer, it is possible to immediately analyze the patient's condition and severity in the field, not in the laboratory, and to calculate a suitable dosage for supporting fluid therapy. Therefore, in this study, the distribution of red blood cells (RBC) and white blood cells (WBC) were analyzed in normal and diarrhea Hanwoo calves. As a result, Hematocrit (HCT) levels increase significantly between 1 and 20 days in diarrhea positive calves. Changes in leukocyte composition had similar growth patterns in normal and diarrhea calves. As it grew, the proportion of neutrophils decreased and lymphocytes increased. However, the number of WBCs increased from 1 to 10 and 21 to 30 days in diarrhea positive calves, which is closely related to the increase in neutrophils. Therefore, those data can be used for diagnosis and treatment of diarrhea calf.

• Journal of Animal Science and Technology
• /
• 제55권5호
• /
• pp.381-388
• /
• 2013

친환경 축산을 통한 고품질의 축산물 생산을 목표로 세계적인 트랜드에 부합하는 축산선진국으로 나아가기 위하여 본 시험은 한우의 군집두수에 따른 우방내 낮은 사육밀도의 정도가 증체, 행동형태 및 스트레스 민감도에 미치는 영향을 비교 분석해 보았다. 강원대학교 부속 동물사육장의 한우 육성우 54두를 공시하여 성별에 따라 군집두수별 4두, 3두 및 2두로 처리하여 각각 3반복을 실시하였으며, 한우 사양관리 프로그램에 따라 사료급여를 실시하였다. 본 시험의 결과로 사양성적 (BW, ADG 및 FCR)의 경우 암소 육성우는 10개월령을 제외한 모든 개월령에서 군집두수가 증가할수록 유의적으로 향상되었으며, 거세우의 경우도 유사한 결과를 나타냈다. 행동관찰에 따른 육성우의 행동시간 발현에서 암소의 경우, 횡와시간은 3두 처리구에서, 거세우의 경우 기립시간은 3두 처리구, 횡와시간은 2두 처리구, 걷기시간 4두 처리구에서 각각 유의적 (p<0.05)으로 길게 나타났다. 행동빈도는 암소에서 음수, 자기 몸핥기, 핥아주기, 투쟁행동에 있어 각각 2두 처리구가 유의적 (p<0.05)으로 낮게 나타났고, 거세우는 음수, 몸 비비기 및 투쟁행동에서 군집두수가 증가함에 따라 유의적 (p<0.05)으로 빈도수가 높아지는 것으로 나타났다. 혈액성상에 있어서 육성암소는 2두 처리구에서 백혈구와 콜티졸 수치가 유의적 (p<0.05)으로 높았고, 글로블린은 유의적 (p<0.05)으로 낮게 나타났다. 이상의 결과를 종합하면 사양성적, 행동특성 및 혈액성상 등의 전체적인 내용을 토대로 표준우사의 우방크기를 고려할 때 한우 육성우의 군집두수는 2두 및 3두 대비 4두로 수용하는 것이 적절하다고 판단되며, 본 시험결과는 추후 친환경 복지형 한우 육성우 사양관리의 기초자료로 활용할 수 있을 것으로 판단된다.

• Journal of Animal Science and Technology
• /
• 제65권1호
• /
• pp.149-159
• /
• 2023

The purpose of this study was to evaluate the effects of castration method and short-term fattening periods on Hanwoo cattle. Thirty-two Hanwoo calves (average body weight: 148.4 ± 19.8 kg) were used. The calves were randomly assigned in a 2 × 2 factor according to two castration methods (castration or hemi-castration) and two short-term finishing periods (24 months or 26 months). The final body weight increased significantly as the fattening period increased (p < 0.05), and the average daily weight gain was higher in the hemi-castration groups than in the castration groups (p < 0.05). After surgical castration, testosterone concentration was higher in the hemi-castration group than in the castration group during the entire experimental period (p < 0.05). The rib eye area was wider in the hemi-castration group than in the castration group (p < 0.01).An interaction effect was observed between the castration method and short-term fattening period (p < 0.05). Marbling and auction price scores differed between castration methods and were higher in the castration group than in the hemi-castration group (p < 0.01). Gross receipts and net income increased in the castration group compared to the hemi-castration group (p < 0.01) and showed a tendency to increase with the short-term fattening period, but there was no significant difference. Thus, castration to remove both testicles is essential for economic profit through producing high-quality Korean beef meat, and short-term fattening for 26 months, rather than 24 months, can benefit more by increasing carcass weight and meat quality.

• 한국동물위생학회지
• /
• 제45권4호
• /
• pp.293-304
• /
• 2022

Among several diseases of calves, diarrhea is the most prevalent disease and has been a major cause of economic loss to the cattle industry. The main etiologic agents of diarrhea in calves are bacteria, viruses, and protozoa, but non-infectious factors including foreign bodies obstruction in the digestive system also focused as the cause of calf diarrhea in the recent days. Because there is still limited information for foreign body-related diarrhea in calves, especially in Hanwoo, the present retrospective study reviewed the medical records for diarrheic calves with foreign body in the digestive system (n=32). The morbidity was determined as 3.03% and more than half of them presented the acidosis, hyponatremia, and azotemia. The mortality in laparotomy-operated calves to remove foreign bodies or in non-operated ones was 28% or 85.7%, respectively, implied the importance of aggressive decision for laparotomy when the foreign bodies were determined in the digestive system in diarrheic calves. During laparotomy, trichobezoars (hair balls) and hays were the main foreign bodies and prevalently placed in the abomasum. In the trials to predict prognosis by several clinical factors, the time for laparotomy over 2 days after first diagnosis, acidosis, and foreign body in the abomasum were highly associated with mortality. Therefore, we believe that prompt surgical procedure (laparotomy) is necessary upon obstruction in the digestive system by foreign bodies is tentatively diagnosed in the diarrheic calf. In addition, when differential diagnosis list is made, foreign body-related diarrhea is necessary to be included in case of diarrheic calf.

• Journal of Animal Science and Technology
• /
• 제49권1호
• /
• pp.59-66
• /
• 2007

본 연구는 분만계절이 포유기 및 이유후 한우 송아지의 음수량, 영양소 섭취능력, 발육 및 질병발생에 미치는 영향을 조사하기 위하여 수행되었다. 시험축은 송아지 67두를 공시하여 분만계절에 따라 봄 분만 송아지 27두와 가을 분만 송아지 40두를 배치하였다. 포유기 건물섭취량은 가을분만 송아지의 경우 생후 3～4개월령에는 동절기 저온 환경의 영향으로 봄 분만 송아지에 비해 감소하였다. 가을 분만 송아지의 생시～2개월령에서, 봄 분만 송아지의 경우 생후 2～4개월령에서 음수량이 증가하였다. 봄 분만 송아지와 가을 분만 송아지의 체중 차이는 없었으나, 생후 2개월령 이후 봄 분만 송아지의 일당증체량이 가을 분만 송아지에 비해 많았다. 생후 60일 이전에 설사 발생이 많았는데, 분만계절별로는 생후 30일 이전에 가을 분만 송아지에서 설사 발생이 많았다. 따라서 한우 포유우사의 분만계절별 사육환경 온도는 송아지의 음수량, 보조사료 섭취량 및 성장발육에 영향을 미치므로 육성률 향상을 위해 포유기 송아지 관리의 차별화가 필요한 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제30권10호
• /
• pp.1425-1434
• /
• 2017

Objective: The main objective of this study was to determine the effect of different diets for early-weaned (EW) calves on rumen development, and how this affects fat deposition in the longissimus dorsi of adult Korean Hanwoo beef cattle. Methods: Three EW groups were established (each n = 12) in which two- week-old Hanwoo calves were fed for ten weeks with milk replacer+concentrate (T1), milk replacer+concentrate+roughage (T2), or milk replacer+concentrate+30% starch (T3); a control group (n = 12) was weaned as normal. At six months, 5 calves of each group were slaughtered and their organs were assessed and rumen papillae growth rates were measured. The remaining calves (n = 7 in each group) were raised to 20 months for further analysis. Results: Twenty-month-old EW calves had a higher body weight (BW), backfat thickness (BF), longissimus dorsi muscle area (LMA) and intramuscular fat (IMF) than the control (p<0.05). Organ growth, rumen histology, and gene expression patterns in the 6-month-old calves were positively related to the development of marbling in the loin, as assessed by ultrasound analysis (p<0.05). In the group fed the starch-enriched diet (T3), higher BW, BF, LMA, and IMF were present. The IMF beef quality score of 20-month-old cattle was 1+ for the T2 and T3 diets and 1 for the T1 diet (p<0.05). Conclusion: Papillae development was significantly greater in calves fed on high-concentrate diets and this may have resulted in the improved beef quality in the EW dietary groups compared to the control.

• 농업생명과학연구
• /
• 제50권2호
• /
• pp.117-124
• /
• 2016

본 연구에는 경남의 혈통등록우 경매시장 A, B 지역에서 2014년도부터 2015년도에 경매된 한우송아지 중에서 경매가격이 있는 19,592두의 경매가격자료를 이용하였고 경매가격에 미치는 환경요인 및 요인별 기여도를 추정하였다. 연구의 결과를 살펴보면 송아지경매가격에 대한 모든 효과에서 고도의 유의적인 차이(p<0.01)를 나타내었고, 준부분 상관제곱(semi-partial correlation)값을 이용한 요인별 기여도 분석에서 회귀식의 수정모형 적합도(Adj R-Square)가 0.701로 나타났으며, 성, 경매체중, 출품개월령, 경매년도, 경매계절 및 경매지역의 준부분상관값은 각각 0.11563, 0.20013, 0.02823, 0.10727, 0.00330 및 0.02963으로 나타났다. 요인별 기여도에서 송아지 체중의 기여도가 가장 높은 것으로 나타났으며, 성, 경매년도, 경매지역 및 출품개월령 순으로 영향을 미치는 것으로 나타났다. 송아지 생산농가에서 경매가격을 잘 받기 위해서는 송아지의 발육정도를 측정할 수 있는 체중의 개량 및 적절한 사양 관리가 매우 중요할 것으로 사료된다. 또한 향후에는 개량방향과 맞는 우수한 유전자의 생산 및 확보가 농가 경쟁력 및 소득증대에 이바지할 것으로 사료된다. 송아지경매가격 및 요인별 기여도에 대한 연구는 아직 많이 부족한 상황으로 더 많은 연구가 이루어져야 할 것으로 사료된다.