• The Korean Journal of Nuclear Medicine Technology
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• v.13 no.3
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• pp.143-146
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• 2009

Purpose: We use the centrifugation of refrigeration state in separation of blood serum, Anti-ds-DNA, Vitamin $B_{12}$/Folate and GAD-Ab assay. However, Cortisol urine and 25-Hydroxyvitamin $D_3$ ($25OHD_3$) are conducted centrifuge at room temperature. This is troublesome that change centrifugation temperature into room temperature due to using of most assays at cold temperature. Therefore when using centrifuge after extraction of Cortisol urine and $25OHD_3$, we conducted researches on effect of the centrifugation temperature in assay results. Materials and Methods: In Cortisol urine, add dichloromethane 1.0 mL in urine $500\;{\mu}L$, mix for 15 minutes, and then centrifuge for 8 minutes at 2600rpm. In $25OHD_3$ add acetonitrile 0.5 mL in serum $200\;{\mu}L$, and then centrifuge for 8 minutes at 2600rpm. Those experiments were conducted centrifuge at room temperature and $4^{\circ}C$. And experiments conducted immediately after centrifugation at $4^{\circ}C$ and standing for 20 minutes after centrifugation $4^{\circ}C$. Results: In Cortisol urine, room temperature result in 1.93, 2.18, 2.43, 9.45, 14.2 (${\mu}g/dL$). Experiments of performing immediately after centrifuge at $4^{\circ}C$ result in 1.8, 2.0, 2.3, 8.1, 13.7 (${\mu}g/dL$). Experiments of performing after 20 minutes result in 2.1, 2.1, 2.7, 9.95, 14.35 (${\mu}g/dL$). On the other hand, the $25OHD_3$ tests conducted at room temperature result in 7.13, 26.6, 35.8, 48.2, 74.8 (ng/dL). Experiments were conducted immediately by pipetting after $4^{\circ}C$ centrifugation result in 7.53, 30.9, 40.3, 61.5, 89.1 (ng/dL) as results are higher than experiments at room temperature. The experiments that conducted centrifuge at $4^{\circ}C$ and then left at room temperature for 20 minutes result in 7.40, 32.4, 41.3, 51.6, 85.6 (ng/dL). Conclusions: Experiments were conducted by using centrifuge at $4^{\circ}C$ are higher or lower than room temperature. The differences between results of standing for 20 minutes after centrifuge at $4^{\circ}C$ and those of centrifuge at room temperature are less than conducting immediately. It is concerned that experiments conducted immediately after centrifuge at $4^{\circ}C$ are incorrect, because tubes become dim due to temperature differences between $4^{\circ}C$ and room temperature. Therefore, it is desirable to centrifuge at room temperature as manual and we should pipet promptly without stopping.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.11
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• pp.259-272
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• 2019

Steel-concrete single-leaf blast-resistant doors are protective structures consisting of a steel box and reinforced concrete slab. By the domestic blast-resistant doors, the structure is not designed efficiently because few studies have examined the effects of variables, such as the blast pressure, rebar ratio, and steel plate thickness on the structural behavior. In this study, the structural behavior of the doors was analyzed using the FE method, and the support rotation and ductility ratio used to classify the structural performance were reviewed. The results showed that the deflection changes more significantly when the plate thickness increases than when the rebar spacing is a variable. This is because the strain energy absorbed by the door is reduced considerably when the plate thickness increases, and as a result, the maximum deflection becomes smaller. According to a comparison of the calculated values of the support rotation and the ductility ratio, the structural performance of the doors could be classified based on the support rotation of one degree and ductility ratio of three. On the other hand, more explosion tests and analytical studies will be needed to classify the damage level.

• International Journal of Contents
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• v.10 no.1
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• pp.54-61
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• 2014

In this paper, we propose a pioneering concept of DPD(Disaster Prevention Design) to realize a securable society in the future. Features of danger in the future society are expected to be diverse, abrupt occurring, large scale, and complicated ways. Due to increment of dangers with their features of uncertainty, interactivity, complexity, and accumulation, human-oriented design concept naturally participates in activities to prevent our society against disasters effectively. We presented DPD is an essential design activity in order to cope with dangers expected in the future societies as well as realize securable environments. DPD is also an integrated design aids including preemptive protections, rapid preparing, recovery, and interactive cooperation. We also expect these activities of DPD is effective for generation of new values in the market, satisfaction of social needs, expansion of design industry, and a novel chance for development in the future society. Throughout this paper, we submit various aspects of DPD concepts including definition, classification, scope, necessity, strategy, influencing elements, process, and its principle. We expect these concepts will be the seed and/or basement of DPD research for the future works. For the direction of study for DPD in the future, we emphasize alarm system for preemptive protection rather than recovery strategy for the damage occurred. We also need to research about progressive prevention techniques and convergence with other areas of design. In order to transfer the concept of product design from facility-oriented mechanism to human-oriented one, we should develop new kinds of city basis facilities, public-sense design concepts referred to social weak-party, e-Learning content design preparing disasters, and virtual simulation design etc. On the other hand, we have to establish laws and regulations to force central and/or provincial governments to have these DPD strategies applying their regional properties. Modern design activities are expanding to UI(user interface) content design area overcoming the conventional design concept of product and/or service. In addition, designers are recognized as art directors or life stylists who will change the human life and create the social value. DPD can be divided into prevention design, preparedness design, response design, and recovery design. Five strategies for successful DPD are Precaution-oriented, Human-oriented, Sense-oriented, Legislation, and Environment Friendly Strategies.

• Korean Journal of Poultry Science
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• v.36 no.3
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• pp.239-246
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• 2009

Animal welfare is of considerable importance to European consumers and citizens, this being most recently confirmed in EU barometer studies. Researchers and others have long proposed that animal-based measures (measures taken on animals, e.g. their health and behaviour) can provide a valid indicator of animal welfare; since welfare is a characteristic of the individual animal. Therefore, a welfare assessment can be essentially based on animal-based measures, but with use of resource measures to provide the capacity to assess 'risk factors'. The first goal of this project was to develop a welfare monitoring system that enables assessment of welfare status through standardised conversion of welfare measures into accessible and understandable information. The acquired information on one hand provides feedback to animal unit managers about the welfare status of their animals, and on the other, information on the welfare status of animal-related products for consumers and retailers. The second goal of Welfare $Quality^{(R)}$ was to improve animal welfare by minimising the occurrence of harmful behavioural and physiological states, improving human-animal relationships, and providing animals with safe and stimulating environments. The different measurable aspects of welfare to be covered are turned into welfare criteria. The criteria reflect what is meaningful to animals as understood by animal welfare science. Once all the measures have been performed on an animal unit, a bottom-up approach is followed to produce an overall assessment of animal welfare on that particular unit: first the data collected (i.e. values obtained for the different measures on the animal unit) are combined to calculate criterion-scores; then criterion-scores are combined to calculate principle-scores; and finally the animal unit is assigned to a welfare category according to the principle-scores it obtained.

• Journal of the Korean Society of Groundwater Environment
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• v.5 no.1
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• pp.1-9
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• 1998

Schlumberger soundings and dipole-dipole electrical surveys were carried out in and around the Nanjido waste landfill in August and December, 1995. Survey points were set to be identical as those in August, 1994 and February, 1995 as possible. To elucidate the annual variation of resistivity structure in and around the landfill, 50 electrical soundings and 2 lines of dipole-dipole surveys were conducted. Interpretations of these data show that mean resistivity values become lower and thicknesses of contaminated layers by leachate thicker than those of the previous year in and around the landfill. Especially, mean thicknesses of saturated layers with leachate increased by about 3∼6 m and resistivities of bedrock decreased. Considering actual hydraulic conductivity, such increments of mean thicknesses are somewhat large. But resistivity variations in and around the Nanjido landfill clearly indicates contamination of layers by leachate is in progress even though some errors in measurements and interpretations are considered. On the other hand, sounding data in the back area of the landfill are almost identical to those of the previous year. From these results, it appears that contamination of weathered zone and bed rock is in progress mainly inside and in the front area of the landfill.

• The Korean Journal of Pharmacology
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• v.31 no.3
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• pp.291-297
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• 1995

The objectives of this study is to compare the inhibitory mechanism of sodium nitroprusside and forskolin on the phorbol ester, activator of protein kinase C (PKC), -induced contractions in rat aorta. $0.1\;{\mu}M$ phorbol dibutyrate (PDBu) induced sustained contractions and increased phosphorylations of myosin light chain (MLC) time-dependently. At 30 min, the contractions and phosphorylations of MLC by PDBu were augmented maximally and remained constant. Moreover, $^{45}Ca^{2+}$ uptake was increased 30 min after PDBu stimulation from resting values. Sodium nitroprusside which activates guanylyl cyclase followed by increasing cGMP, inhibited the PDBu-induced contractions concentration-dependently. On the other hand, forskolin which activates adenylyl cyclase followed by increasing cAMP, also inhibited the PDBu-induced contractions concentration-dependently. However, sodium nitroprusside was more potent to inhibition of the PDBu-induced contractions than forskolin. Sodium nitroprusside inhibited $^{45}Ca^{2+}$ uptake by PDBu stimulation. Forskolin also inhibited $^{45}Ca^{2+}$ uptake by PDBu stimulation. Sodium nitroprusside and forskolin inhibited the phosphorylations of MLC by PDBu, respectively. However, sodium nitroprusside was more potent to inhibition of phosphorylations of MLC by PDBu than forskolin. From these results, Sodium nitroprusside via cGMP or forskilin via cAMP may reduce myoplasmic $Ca^{2+}$ followed by suppression of phosphorylations of MLC of PKC-mediated contractions, which results in vasodilation. However, cGMP may play a role more importantly than cAMP on the regulation of protein kinase C-mediated contraction in vascular smooth muscle.

• The Korean Journal of Pharmacology
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• v.18 no.1
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• pp.55-63
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• 1982

Lipid peroxidation is the reaction of oxidative deterioration of polyunsaturated lipids and this peroxidation involves the direct reaction of oxygen and lipid to form free radical intermediates, which can lead to autocatalysis. As results of the extensive studies on the lipid peroxidation by many authors, the relationship between lipid peroxidation and the drug metabolizing system as well as the actions of free radicals on the peroxidation was reasonably well known. For a long time, the mechanism of hepatotoxicity of $CCl_4$ was not clearly understood. However, it is now quite well established that $CCl_4$ is activated in vivo to a free radical which is a highly reactive molecule. Therefore, lipid peroxidation which induces the reduction of cytochrome P-450 and aminopyrine demethylase activity is known as decisive event of $CCl_4$ hepatotoxicity. On the other hand, it was also reported that singlet molecular oxygen produces lipid peroxidation in liver microsomes. In this study the effects of benzoyl peroxide on the lipid peroxidation and drug-metabolizing enzyme were examined. Benzoyl peroxide mixed with starch and phosphates etc. is usually used as a food additive for flour bleaching and maturing purpose because of its oxidative property. Albino rats were used for the experimental animals. Benzoyl peroxide was suspended in soybean oil and sesame oil and administered intraperitoneally or orally. TBA value and aminopyrine demethylase activity were determined in liver microsomal fraction and serum. The results were summerized as following. 1) Body weights of animals administered benzoyl peroxide suspension were decreased while that of oil administered group were increased. 2) The activity of aminopyrine demethylase was generally decreased in animals administered oil suspension of benzoyl peroxide. Furthermore, the marked reduction of the enzyme activity was observed in animals administered benzoyl peroxide intraperitoneally. 3) Generally, microsomal TBA values as well as serum TBA were significantly elevated in benzoyl peroxide group in comparison with the control group. However, the more remarkable increase of serum TBA than microsomal TBA was observed in animals administered orally for 6 days. 4) Specifically, the changing pattern of TBA value was notable in serum rather than in liver microsome by intraperitoneal administration of benzoyl peroxide.

• The Journal of the Korea Contents Association
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• v.18 no.2
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• pp.553-568
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• 2018

Popular music is a spce where intrinsic desires and values of young people are well exposed in its process of production and consumption. Previous discussions of Korean youth and youth culture have been proceeding mainly in the sense of their resistances to older generation. However, this is limited in that it neglects the inherent microscopic characteristics of youth culture. The purpose of this study is to investigate what the signs and discourses of youth generation make and share in popular music, and how it relates to social context through the 'Mimesis' theory. As a result, the songs of the 1990s remarkably showed the resistance to the old generation in its narrative structure and have signs which expresses repression and uniformity. On the other hand, the songs of the 2010s have many fragmented signs which has been reconstructed into individualized discourse structure. It is assumed that the songs have been able to gain a lot of empathy and popularity from the young generation by visualizing emotions and desires experienced by themselves in the social context. Through this study, it suggests to pay attention to the sensitivity, desire and creativity of young people in music in the social and cultural context of the time.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.47 no.4
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• pp.281-289
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• 2011

The purpose of this study is to identify the flow resistance of the bottom trawl net. The bottom trawl net being used in the training ship of Chonnam National University was selected as a full-scale net, and model nets such as 1/10, 1/25 and 1/50 of the actual net were made. Total resistance of the net part, the height of the net mouth and the flow resistance of components of the net such as wing, bag and cod-end part was measured, converted into full-scale and compared. Additionally, the model rule of Tauti (1934), which has been most frequently used in fishing net modeling experiments, was applied to interpret flow resistance and scale effect of model experiment was investigated. Presumed that the flow resistance R is $R=kS{\upsilon}^2$ against the flow velocity of each net ${\upsilon}$, resistance coefficient k was calculated by substituting R, ${\upsilon}$ and S of the net. From the result, it was found that k decreases exponentially when u increases which makes $k=c{\upsilon}^{-m}$. Whereas m of each net is ranged between 0.13-0.16 and there was not significant difference between nets. c does not show big difference in 1/10 and 1/25 model and the value itself was relatively bigger than in 1/50 model. The height of the net mouth of 1/25 and 1/50 model net h decreases exponentially according as ${\upsilon}$ increases to make $h=d{\upsilon}^{-n}$. Whereas d and n values were almost same in two nets. Additionally, when resistance of cod-end, wing and bag part in 1/25 and 1/50 model nets, both nets showed big resistance in bag part when flow is 1m/s as more than 60%. Wing and cod-end part showed almost same value or wing part had little bit larger value. On the other hand, when reviewing the reasons why both models showed difference in 1/50 model while c value against the resistance coefficient k did not show big difference in 1/10 and 1/25 model, it is inferred that the difference occurred not from material difference but from the difference in net size according to scale. It was judged that they are the scale effects concomitant to the model experiments.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.2
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• pp.163-168
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• 2007

Ecological risk assessment(ERA) to 5 abandoned mine drainage was investigated by using chemical measurement and bioassay experiment. From the results of chemical analysis, the high concentration of heavy metals are detected in most area. The Arsenite were mostly detected in Songcheon, Nakdong, and Dukum abandoned mine area, and various heavy metals were highly dispersed in Nakdong area. The study area have also high biological toxicity, resulted from the bioassay based on WET(Whole Effluent Toxicity) test by using Vibrio fisheri, Selenastrum copricornutum, and Daphnia magna. The maximum toxicity was shown in the point where the mine waters start to flow. The sensitivity of toxicity by S. capricornutum was relatively high considering the values of toxicity in all samples, from 1.3 to 32.0 TU. The different sensitivities of toxicity recommends the use of battery system, resulted from at least two test species for bioassay or ecological risk assessment of mine drainage. Besides, the results showed high hazard quotient(i.e., greater than 1 HQ value indicating potentially significant toxic risks) with regard to abandoned mine drainage area in this study. On the other hand, the biological toxicity results were sharply decreased by attenuation along further distance from discharging of mine waters. Therefore, environmental parameters including the dilution factor, dissolved organic matter, and hardness should be considered when the remediation and ERA of abandoned mine drainage is planned.